NaCl胁迫对3种饲草种子萌发的影响

doi:10.6048/j.issn.1001-4330.2023.02.023

摘要/Abstract

摘要：

【目的】 研究新引进饲草品种的耐盐能力,分析其耐盐浓度范围,筛选耐盐能力强的品种,提高饲草产量和盐碱地利用率。【方法】 以引进的饲料油菜、饲用甜高粱、紫花苜蓿新品种为研究对象,分别用不同浓度NaCl溶液0%、0.4%、0.8%、1.2%、1.6%胁迫处理,测定7 d内的发芽率、发芽势、相对盐害率、发芽势盐害率、萌发指数。【结果】 与对照0%处理相比,在NaCl浓度为0~0.4%时,3种饲草种子的发芽率、发芽势均无显著变化。饲用甜高粱在低盐胁迫时较敏感,NaCl浓度为0.4%时,相对盐害率为53.41%,萌发指数为28;NaCl浓度为0.8%,紫花苜蓿的发芽率最高,为96.67%。饲料油菜的发芽率次之,为93.33%。饲用甜高粱的发芽率最低,为75.33%,发芽势盐害率最高,为18.57%,盐胁迫抑制较明显,萌发指数最低,为20.89;NaCl浓度为1.2%时,饲料油菜的的发芽率最高,为 83.33%,相对盐害率达100%,饲用甜高粱和紫花苜蓿的相对盐害率分别为93.4%和67.68%。NaCl浓度为1.6%时,紫花苜蓿的发芽率最高,为74.67%,盐害率最低,为81.61%。【结论】 饲用甜高粱对低盐胁迫较敏感,饲料油菜在中、高浓度盐胁迫时的萌发抑制明显,紫花苜蓿对盐害的耐受最强,高盐胁迫下仍能保持最高的发芽率、发芽势。3种饲草对NaCl盐胁迫的耐受程度为紫花苜蓿>饲料油菜>饲用甜高粱。

关键词: 饲草; 盐胁迫; 种子萌发

Abstract:

【Objective】 To study the salt tolerance of newly introduced forage varieties, the range of salt tolerance concentration, and screen out the varieties with strong salt tolerance, which is very important to improve salinized soil and land efficiency. 【Methods】 The new varieties of forage rape, sweet sorghum and alfalfa were taken as the research objects, different concentrations of NaCl solution (0%,0.4%,0.8%,1.2%,1.6%)were used for stress treatment to determine the germination rate, germination potential, relative salt damage rate,salt damage rate of germination potential and germination index within 7 days. 【Result】 Compared with the control 0% treatment,when the concentration of NaCl was 0-0.4%, there was no significant difference in germination rate and germination potential among the three forages. Sweet sorghum was more sensitive to low salt stress, when the concentration of NaCl was 0.4%, the relative salt damage rate was 53.41%, and the germination index was 28; When the concentration of NaCl was 0.8%, the germination rate of alfalfa was the highest, which was 96.67%. The germination rate of forage rape was 93.33%. The germination rate of forage sweet sorghum was the lowest, which was 75.33%. Otherwise, the salt damage rate of germination potential was the highest, which was 18.57%. The inhibition of salt stress was obvious, the germination index was the lowest (20.89); When the concentration of NaCl was 1.2%, the germination rate of forage rape was the highest, which was 83.33% and the relative salt damage rate was 100%. The relative salt damage rates of sweet sorghum and alfalfa were 93.4% and 67.68% respectively. When the concentration of NaCl was 1.6%, the germination rate of alfalfa was the highest (74.67%), and the salt damage rate was the lowest (81.61%). 【Conclusion】 In general, forage sweet sorghum is sensitive to low salt stress. The germination of feed rape is obviously inhibited under medium and high concentration salt stress. Alfalfa has the strongest tolerance to salt stress, and maintained the highest germination rate and germination potential under high salt stress. The tolerance order of the three forages to NaCl stress is alfalfa > forage rape > forage sweet sorghum.

Key words: forage; salt stress; seed germination

中图分类号:

杨金钰, 乔小燕, 王西和, 孙九胜. NaCl胁迫对3种饲草种子萌发的影响[J]. 新疆农业科学, 2023, 60(2): 448-453.

YANG Jinyu, QIAO Xiaoyan, WANG Xihe, SUN Jiusheng. Effects of NaCl Stress on Seed Germination of Three Varieties of Forage[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 448-453.

图/表 3

参考文献 24

[1]	王遵亲. 中国盐渍土[J]. 北京: 科学出版社, 1993.
	WANG Zunqin. Chinese Saline Soil[J]. Beijing: Science Press, 1993.
[2]	樊自立, 马英杰, 马映军. 中国西部地区盐渍土及其改良利用[J]. 干旱区研究, 2001, 18(3):1-6.
	FAN Zili, MA Yingjie, MA Yingjun. Salinized Soils and Their Improvement and Utilization in West China[J]. Arid Zone Research, 2001, 18(3):1-6.
[3]	杨劲松. 中国盐渍土研究的发展历程与展望[J]. 土壤学报, 2008, 45(5):837-845.
	YANG Jinsong. Development and prospect of the research on salt-affected soils in China[J]. Acta Pedologica Scina, 2008, 45(5):837-845.
[4]	Liu G M, Li J B, Zhang X C, et al. GIS-mapping spatial distribution of soil salinity for Eco-restoring the Yellow River Delta in combination with Electromagnetic Induction[J]. Ecological Engineering, 2016, 94: 306-314. DOI URL
[5]	傅廷栋, 涂金星, 张毅, 等. 在我国西北部地区麦后复种饲料油菜的研究与利用[J]. 中国西部科技, 2004, 3(6):4-7.
	FU Tingdong, TU Jinxing, ZHANG Yi, et al. Research and utilization of wheat stubble multiple cropping rape in Northwest China[J]. Science and Technology of West China, 2004, 03(6):4-7.
[6]	刘杰淋, 王建丽, 申忠宝, 等. 18个引进紫花苜蓿品种生产性能比较研究[J]. 饲料研究, 2021,(1). https://kns.cnki.net/kcms/detail/11.2114.S.20210121.1701.040.html
	LIU Jielin, WANG Jianli, SHEN Zhongbao, et al. Production performance comparison of eighteen introduced alfalfa varieties[J]. Feed Research, 2021,(1). https://kns.cnki.net/kcms/detail/11.2114.S.20210121.1701.040.html
[7]	唐桃霞, 王致和, 张亚萍, 等. 刈割频率对饲用甜高粱产量和饲用价值的影响[J]. 中国糖料, 2018, 40(1):35-37,39
	TANG Taoxia, WANG Zhihe, ZHANG Yaping, et al. Effect of Mowing Frequency on Yield and Feeding Value of Forage Sweet Sorghum[J]. Chinese Sugar, 2018, 40(1):35-37,39
[8]	冯帆, 李昊, 杨洁, 等. 饲料油菜不同添加比例对滩羊瘤胃体外发酵的影响[J]. 草业科学, 2020, 37(21):2343-2350.
	FENG Fan, LI Hao, YANG Jie, et al. Effects of mixed silage containing corn straw and rape on the in vitro fermentation characteristics of rumen fluid from Tan sheep[J]. Pratacultural Science, 2020, 37(11): 2343-2350.
[9]	阴法庭, 张凤华. 饲料油菜与玉米秸秆混合青贮营养品质[J]. 草业科学, 2018, 35(7): 1790-1796.
	YIN Fating, ZHANG Fenghua. Nutritional quality of silage made with different ratios of forage rape and corn stalk[J]. Pratacultural Science, 2018, 35(7): 1790-1796.
[10]	段玉, 张君, 景宇鹏, 等. 河套灌区麦后复种饲料油菜养分吸收规律及施肥效应[J]. 中国油料作物学报, 2017, 39(6):848-854.
	DUAN Yu, ZHANG Jun, JING Yupeng, et al. NPK absorption,fertilizing effect of wheat /silage rape multiple cropping in Hetao irrigation area[J] .Chinese Journal of Oil Crop Science, 2017, 39(6):848-854.
[11]	Wang L, Xie J, Luo Z, et al. Forage yield, water use efficiency, and soil fertility response to alfalfa growing age in the semiarid Loess Plateau of China[J]. Agricultural Water Management, 10.1016/J.AGWAT.2020.106415. DOI
[12]	Achal Shambharkar G K,. Londhe V G. Atkare. Effect of feeding sweet sorghum fodder on milk yield and composition of crossbred (HF x Deoni) cows[J]. Indian Journal of Animal Research, 2018, 52(6):929-933.
[13]	李卫明, 许辉欣, 柴政, 等. 不同浓度盐胁迫对三种饲草作物种子萌发特性的影响[J]. 中国奶牛, 2019,(3):62-65.
	LI Weiming, XU Huixin, CHAI Zheng, et al. Influence of salt concentration on germination of three different forage crop seeds[J]. China Dairy, 2019,(3):62-65.
[14]	胡宗英, 张红香, 孙泽威. 盐碱胁迫对农牧作物种子萌发的影响研究进展[J]. 中国种业, 2014,(5):21-23.
	HU Zongying, ZHANG Hongxiang, SUN Zewei. Research progress on effects of saline alkali stress on seed germination of agricultural and animal husbandry crops[J]. Seed Industry of China, 2014,(5): 21-23
[15]	郝培彤, 李玉龙, 栾瑞涛, 等. 21份引进BMR饲草高粱萌发期苗期耐旱耐盐性评价[J]. 草业科学, 2018, 35(5):1199-1207.
	HAO Peitong, LI Yulong, LUAN Ruitao, et al. Evaluation of drought and salt stress during the germination and seedling stages of 21 introduced brown midrib forage sorghum species[J]. Pratacultural Science, 2018, 35(5):1199-1207.
[16]	李源, 朱倩倩, 许咏梅. NaCl胁迫对几种典型耐盐经济作物种子萌发的影响[J]. 中国土壤与肥料, 2020,(2):226-231.
	LI Yuan, ZHU Qianqian, XU Yongmei. Effects of salt stress on seed germination of different cash crops[J]. Soil and Fertilizer Sciences in China, 2020,(2):226-231.
[17]	王传旗, 德吉卓玛, 张佳佳, 等. 盐胁迫对西藏3种野生披碱草属牧草种子发芽影响[J]. 草业与畜牧, 2016,(6):17-22.
	WANG Chuanqi, DEJI Zhuoma, ZHANG Jiajia, et al. Salt stress on three wild elymus forages germination of Tibet[J]. Pratacultural & Animal Husbandry, 2016,(6):17-22.
[18]	申吴燕, 吐尔逊娜依, 雪热提江, 等. 12种植物萌发期耐盐性筛选[J]. 新疆农业科学, 2020, 57(10):1912-1920. DOI
	SHEN Wuyan, Tuerxunnayi, Xueretijiang, et al. Screening of salt tolerance of 12 plant species at germination stage[J]. Xinjiang Agricultural Sciences, 2020, 57(10):1912-1920. DOI
[19]	景鹏成, 王树林, 陈乙实, 等. 耐盐牧草对南疆地区盐渍土的适应和改良研究[J]. 草业学报, 2017, 26(10):56-63.
	JING Pengcheng, WANG Shulin, CHEN Yishi, et al. Adaptation of salt-tolerant forage grasses to saline soil and their ability to improve saline soil utilization in southern Xinjing region[J]. Aeta Prataculturae Sinica, 2017, 26(10):56-63.
[20]	高凤菊, 曹鹏鹏, 王乐政, 等. 盐度对不同类型甜高粱品种萌发的影响[J]. 山东农业科学, 2011(11):44-47.
	GAO fengju, CAO Pengpeng, WANG Lezheng, et al. Effects of salinity on Germination of different sweet sorghum varieties[J]. Shandong Agricultural Sciences, 2011(11):44-47.
[21]	张利霞, 常青山, 侯小改, 等. 不同钠盐胁迫对夏枯草种子萌发特性的影响[J]. 草业学报, 2015, 24(3):177-186.
	ZHANG Lixia, CHANG Qingshan, HOU Xiaogai, et al. Effects of sodium salt stress on seed germination of Prunella vulgaris[J]. Acta Prataculturae Sinica, 2015, 24(3):177-186.
[22]	季波, 徐金鹏, 时龙, 等. 10种禾本科牧草种子萌发期耐盐性[J]. 新疆农业科学, 2021, 58(2):342-351. DOI
	JI Bo, XU Jinpeng, SHI Long, et al. Salt resistance of 10 grasses at germination stage[J]. Xinjiang Agricultural Sciences, 2021, 58(2):342-351. DOI
[23]	武鹏雨, 马治浩, 张锐. 和田地区核桃林下饲料油菜间作模式探析[J]. 新疆农垦科技, 2020, 43(12):15-17.
	WU Pengyu, MA Zhihao, ZHANG Rui. Study on Intercropping Pattern of forage rape under walnut forest in Hotan Area[J]. Xinjiang Agricultural Reclamation Science and Technology, 2020, 43(12):15-17.
[24]	张妙娟, 吴琼, 张引弟. NaCl胁迫对油菜幼苗生长的影响研究[J]. 种子科技, 2020, 38(23):11-14.
	ZHANG Miaojuan, WU Qiong, ZHANG Yindi. Effects of NaCl stress on growth of rape seedlings[J]. Seed Science and Technology, 2020, 38(23):11-14.

Nacl处理 Nacl treatment (%)	饲料油菜Forage		饲用甜高粱Sweet sorghum		紫花苜蓿Alfalfa
Nacl处理 Nacl treatment (%)	发芽率 Germination rate	相对盐害率 Relative salt damage rate	发芽率 Germination rate	相对盐害率 Relative salt damage rate	发芽率 Germination rate	相对盐害率 Relative salt damage rate
0	97.33±1.15^a	0^c	92.67±3.06^a	0^d	100^a	0^e
0.4	91.33±6.43^ab	32.58±12.22^b	85.33±3.06^a	53.41±6.11^c	98.67±2.31^a	27.12±8.80^d
0.8	93.33±3.06^ab	96.3±6.41^a	75.33±3.06^b	77.12±14.43^b	96.67±4.16^a	46.36±2.73^c
1.2	83.33±2.31^b	100^a	70.00±2.00^b	93.40±5.77^a	77.33±2.31^b	67.68±5.20^b
1.6	70.67±3.06^c	100^a	58.67±9.02^c	98.96±1.80^a	74.67±4.16^b	81.61±1.02^a

Nacl处理 Nacl treatment (%)	饲料油菜Forage		饲用甜高粱Sweet sorghum		紫花苜蓿Alfalfa
Nacl处理 Nacl treatment (%)	发芽率 Germination rate	相对盐害率 Relative salt damage rate	发芽率 Germination rate	相对盐害率 Relative salt damage rate	发芽率 Germination rate	相对盐害率 Relative salt damage rate
0	97.33±1.15^a	0^c	92.67±3.06^a	0^d	100^a	0^e
0.4	91.33±6.43^ab	32.58±12.22^b	85.33±3.06^a	53.41±6.11^c	98.67±2.31^a	27.12±8.80^d
0.8	93.33±3.06^ab	96.3±6.41^a	75.33±3.06^b	77.12±14.43^b	96.67±4.16^a	46.36±2.73^c
1.2	83.33±2.31^b	100^a	70.00±2.00^b	93.40±5.77^a	77.33±2.31^b	67.68±5.20^b
1.6	70.67±3.06^c	100^a	58.67±9.02^c	98.96±1.80^a	74.67±4.16^b	81.61±1.02^a

Nacl处理 Nacl treatment (%)	饲料油菜Forage		饲用甜高粱Sweet sorghum		紫花苜蓿Alfalfa
Nacl处理 Nacl treatment (%)	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential
0	97.33±1.15^a	0^c	92.67±3.06^a	0^c	100^a	0^b
0.4	91.33±6.43^a	6.13±7.07^c	85.33±3.06^a	7.87±3.27^c	98.67±2.31^a	1.33±2.31^b
0.8	93.33±3.06^a	4.13±2.15^c	75.33±3.06^b	18.57±5.94^b	96.67±4.16^a	3.33±4.16^b
1.2	83.33±2.31^b	14.33±3.41^b	70.00±2.00^b	24.40±2.70^b	77.33±2.31^b	22.67±2.31^a
1.6	68.00±2.00^c	30.17±2.21^a	58.67±9.02^c	36.57±10.97^a	74.67±4.16^b	25.33±4.16^a

Nacl处理 Nacl treatment (%)	饲料油菜Forage		饲用甜高粱Sweet sorghum		紫花苜蓿Alfalfa
Nacl处理 Nacl treatment (%)	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential	发芽势 Germination potential	发芽势盐害率 Salt damage rate of germination potential
0	97.33±1.15^a	0^c	92.67±3.06^a	0^c	100^a	0^b
0.4	91.33±6.43^a	6.13±7.07^c	85.33±3.06^a	7.87±3.27^c	98.67±2.31^a	1.33±2.31^b
0.8	93.33±3.06^a	4.13±2.15^c	75.33±3.06^b	18.57±5.94^b	96.67±4.16^a	3.33±4.16^b
1.2	83.33±2.31^b	14.33±3.41^b	70.00±2.00^b	24.40±2.70^b	77.33±2.31^b	22.67±2.31^a
1.6	68.00±2.00^c	30.17±2.21^a	58.67±9.02^c	36.57±10.97^a	74.67±4.16^b	25.33±4.16^a