花后高温对小麦籽粒淀粉合成及相关酶活性的影响

doi:10.6048/j.issn.1001-4330.2018.02.002

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (2): 210-218.DOI: 10.6048/j.issn.1001-4330.2018.02.002

花后高温对小麦籽粒淀粉合成及相关酶活性的影响

赵云¹,李鹏兵¹,唐冬梅²,林静¹,冯宽¹,李卫华¹

1.石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子 832003;
2.石河子农业科学研究院粮油所,新疆石河子 832003

收稿日期:2017-11-25 出版日期:2018-02-20 发布日期:2018-06-30
通信作者: 李卫华(1968-),女,新疆伊犁人,教授,博士生导师,研究方向为小麦品质及分子机理,(E-mail)lwh_agr@shzu.edu.cn
作者简介:赵云(1992-),男,新疆奇台人,硕士研究生,研究方向为作物育种,(E-mail)1041912942@qq.com
基金资助:
新疆兵团科技局现代农业科技攻关与成果转化计划(2016AC027:2015AC015)

Effects of Post-Anthesis High Temperature Stress on Starch Synthesis and Activities of Related Enzymes in Wheat Grain

ZHAO Yun¹, LI Peng-bing¹, TANG Dong-mei², LIN Jing¹, FENG Kuan¹, LI Wei-hua¹

1.Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Corps, College of Agronomy, Shihezi University, Shihezi Xinjiang 832003, China ;
2.Crop and Oil Research Institute of Shihezi, Shihezi Xinjiang 832003,China

Received:2017-11-25 Online:2018-02-20 Published:2018-06-30
Correspondence author: LI Wei-hua(1968-), Professor, Doctoral tutor, mainly engaged in wheat quality and molecular mechanism.(E-mail)lwh_agr@shzu.edu.cn

摘要/Abstract

摘要： 【目的】研究小麦花后高温对籽粒淀粉合成及相关酶活性的影响,为小麦耐高温品种的选育及响应逆境胁迫提供理论依据。【方法】以新疆春小麦主栽品种新春11号为材料,于花后5~8 d进行高温处理,测定籽粒各时期淀粉含量及相关酶活性。【结果】花后高温处理显著降低了小麦籽粒各时期总淀粉、直链淀粉和支链淀粉含量及其积累速率;总淀粉和支链淀粉的积累速率呈单峰曲线变化,而直链淀粉的积累速率则呈现先下降后逐渐上升最后回落的变化趋势。花后高温处理下小麦籽粒各时期蔗糖合成酶(SS)活性、可溶性淀粉合成酶(SSS)活性和淀粉分支酶(SBE)活性显著低于对照,而焦磷酸化酶(ADPGase)活性虽然在各时期均低于对照,但差异不显著。在灌浆中后期SS、SSS、SBE活性与总淀粉含量、支链淀粉含量显著相关。【结论】高温胁迫显著降低了小麦籽粒淀粉合成相关酶的活性,进一步影响到小麦籽粒淀粉的积累。在小麦生产过程中,做好高温防范尤为重要。

关键词: 小麦; 花后高温; 淀粉; 酶活性

Abstract: 【Objective】 The purpose of this project aims to explore the effects of post-anthesis high temperature on starch synthesis and related enzyme activities in wheat grain and provide a theoretical basis for breeding wheat varieties with high temperature tolerance and response to adversity stress.【Method】Xinjiang main cultivar Xinchun 11 was used as the experimental material and the starch content and related enzyme activity in different grain stages were measured at 5-8 d after anthesis.【Result】Post-anthesis high temperature treatment significantly reduced the contents of total starch, amylose and amylopectin and their accumulation rate, and the accumulation rate of total starch and amylopectin showed a single peak curve, while the amylose accumulation rate showed a downward trend and a gradual increase and then a downward trend. The activities of sucrose synthase (SS), soluble starch synthase (SSS), and starch branching enzyme (SBE) in wheat grain in different grain stages were significantly lower than those in the control by post-anthesis high temperature treatment but the difference was not significant. Correlation analysis showed that SS, SSS and SBE activities were significantly correlated with total starch content and amylopectin content during the late filling stage.【Conclusion】High temperature stress significantly reduced the activity of enzymes related to starch synthesis in wheat grain and further affected the starch accumulation in wheat grain. Therefore, in the process of wheat production, it is particularly important to take precautions against high temperatures.

Key words: wheat; post-anthesis high temperature; starch; enzyme activity

中图分类号:

S512

赵云,李鹏兵,唐冬梅,林静,冯宽,李卫华. 花后高温对小麦籽粒淀粉合成及相关酶活性的影响[J]. 新疆农业科学, 2018, 55(2): 210-218.

ZHAO Yun, LI Peng-bing, TANG Dong-mei, LIN Jing, FENG Kuan, LI Wei-hua. Effects of Post-Anthesis High Temperature Stress on Starch Synthesis and Activities of Related Enzymes in Wheat Grain[J]. Xinjiang Agricultural Sciences, 2018, 55(2): 210-218.

参考文献 22

[1]	辛明明, 彭惠茹, 倪中福, 等. 小麦耐热性的生理遗传研究进展[J]. 中国农业科学, 2017,(5):783-791.XIN Ming-ming, PENG Hui-ru, NI Zhong-fu, et al. (2017). Progresses in Research of Physiological and Genetic Mechanisms of Wheat Heat Tolerance [J]. Scientia Agricultura Sinica, 50(5): 783-791. (in Chinese)
[2]	李咏, 赵云, 张辉, 等. 花后高温对小麦农艺性状及面粉品质的影响[J]. 麦类作物学报, 2016,36(11):1 482-1 488.LI Yong, ZHAO Yun, ZHANG Hui, et al. (2016). Effect of High Temperature after Anthesis on Agronomic Characteristics and Flour Quality of Wheat [J]. Microphyllus Crops, 36(11): 1,482-1,488. (in Chinese)
[3]	岳鹏莉, 王晨阳, 卢红芳, 等. 灌浆期高温干旱胁迫对小麦籽粒淀粉积累的影响[J]. 麦类作物学报, 2016,(11):1 489-1 496.YUE Peng-li, WANG Chen-yang, LU Hong-fang, et al. (2016). Effect of Heat and Drought Stress on Starch Accumulation During Grain Filling Stage [J]. Journal of Wheat Crops, 11(36): 1,489-1,496. (in Chinese)
[4]	Kang, G. Z., Xu, W., Liu, G. Q., Peng, X. Q., & Guo, T. C. (2013). Comprehensive analysis of the transcription of starch synthesis genes and the transcription factor rsr1 in wheat (triticum aestivum) endosperm. Genome / National Research Council Canada = Génome / Conseil national de recherches Canada, 56(2):115.
[5]	Jing, F. U., Yun-Ji, X. U., Lu, C., Yuan, L. M., Wang, Z. Q., & Yang, J. C. (2013). Changes in enzyme activities involved in starch synthesis and hormone concentrations in superior and inferior spikelets and their association with grain filling of super rice. Chinese Journal of Rice Science, 20(2): 120-128.
[6]	李淑益. 花后高温、干旱及其复合胁迫对小麦籽粒淀粉合成酶基因表达的影响[D]. 郑州:河南农业大学硕士学位论文, 2013.LI Shu-yi. (2013). Effects of Post-Anthesis High Temperature, Drought and Combined Stress on Starch Synthetase Gene Expression in Wheat Grain [D]. Master Dissertation. Hebei Agricultural University, Zhengzhou.
[7]	姚珊. 花后高温干旱对春小麦淀粉形成的影响[D]. 银川:宁夏大学硕士学位论文, 2013.YAO Shan. (2013)Effects of high temperature and drought after anthesis on starch formation in spring wheat [D]. Master Dissertation. Ningxia University, Yinchuan. (in Chinese)
[8]	Xie, Z., Jiang, D., Cao, W., Dai, T., & Jing, Q. (2003). Effects of post-anthesis soil water status on the activities of kiy regulatory enzymes of starch and protein accumulation in wheat grains. Acta Photophysiologica Sinica, 29(4): 309-316.
[9]	Yan, S. H., Yin, Y. P., Li, W. Y., Li, Y., Liang, T. B., & Wu, Y. H., et al. (2008). Effect of high temperature after anthesis on starch formation of two wheat cultivars differing in heat tolerance. Acta Ecologica Sinica.
[10]	Jenner, CF. (1991). Effects of exposure of wheat ears to high temperature on dry matter accumulation and carbohydrate metabolism in the grain of two cultivars. ii. carry-over effects. Functional Plant Biology, 18(2):179-190.
[11]	Keeling, P. L., Banisadr, R., Barone, L., Wasserman, B. P., & Singletary, G. W. (1994). Effect of temperature on enzymes in the pathway of starch biosynthesis in developing wheat and maize grain. Functional Plant Biology, 21(21): 807-827.
[12]	刘襄河, 郑丽璇, 郑丽勉, 等. 双波长法测定常用淀粉原料中直链淀粉、支链淀粉及总淀粉含量[J]. 广东农业科学, 2013,40(18):97-100.LIU Xiang-he, ZHENG Li-xuan, ZHENG Li-mian, et al. (2013). Determination of amylose and amylopectin in the commonly used starch materials by dual-wavelength spectrophotometry [J]. Guangdong Agricultural Sciences, 40(18): 97-100. (in Chinese)
[13]	陈婷婷, 许更文, 钱希旸, 等. 花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达[J]. 中国农业科学, 2015,(7):1 288-1 299.CHEN Ting-ting, XU Geng-wen, QIAN Xi-yun, et al. (2015). Post-Anthesis Alternate Wetting and Moderate Soil Drying Irrigation Enhance Gene Expressions of Enzymes Involved in Starch Synthesis in Rice Grains [J]. Scientia Agricultura Sinica, (7): 1,288-1,299. (in Chinese)
[14]	程方民, 蒋德安, 吴平, 等. 早籼稻籽粒灌浆过程中淀粉合成酶的变化及温度效应特征[J]. 作物学报, 2001,27(2):201-206.CHENG Fang-min, JIANG De-an, WU Ping, et al. (2001). The Dynamic Change of Starch Synthesis Enzymes during the Grain Filling Stage and Effects of Temperature upon It [J]. Acta Agronomica Sinica, 27(2): 201-206. (in Chinese)
[15]	Schaffer, A. A., & Petreikov, M. (1997). Sucrose-to-starch metabolism in tomato fruit undergoing transient starch accumulation. Plant Physiology,113(3): 739.
[16]	汤章城. 现代植物生理学实验指南[M]. 科学出版社, 1999.TANG Zhang-cheng. (1999). Modern Plant Physiology Experiment Guide [M]. Science Press. (in Chinese)
[17]	Zeng, M., Morris, C. F., Batey, I. L., & Wrigley, C. W. (1997). Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. Cereal Chemistry, 74(1): 63-71.
[18]	张玉峰. 高温胁迫对小麦籽粒蛋白质含量和淀粉含量影响的研究[D]. 北京:中国农业大学博士学位论文, 2016.ZHANG Yu-feng. (2016). Studies on the effect of high temperature stress on the grain protein content and starch content in wheat [D]. PhD Dissertation. China Agricultural University, Beijing. (in Chinese)
[19]	王珏. 花后高温对小麦淀粉结构和特性的影响[D]. 扬州: 扬州大学硕士学位论文, 2008.WANG Jue. (2008). Effect of Post-anthesis High Temperature on Starch Structure and Characteristics of wheat [D]. Master Dissertation. Yangzhou University, Yangzhou. (in Chinese)
[20]	张桂莲, 廖斌, 汤平, 等. 灌浆结实期高温对水稻剑叶生理特性和稻米品质的影响[J]. 中国农业气象, 2014,35(6):650-655.ZHANG Gui-lian, LIAO Bin, TANG Ping, et al. (2014). Effects of High Temperature Stress During Grain-filling Period on Physiological Characteristics in Flag Leaves and Grain Quality of Rice [J]. China Agricultural Meteorology, 35(6): 650-655. (in Chinese)
[21]	Stoddard, F. L. (1999). Survey of starch particle-size distribution in wheat and related species. Cereal Chemistry, 76(1):145-149.
[22]	李木英, 石庆华, 胡志红, 等. 高温胁迫对不同早稻品种胚乳淀粉合成酶类活性的影响[J]. 中国农业科学, 2007,(8):1 622-1 629.LI Mu-ying, SHI Qing-hua, HU Zhi-hong, et al. (2007). Effects of High Temperature Stress on Activity of Amylosynthease in Endosperm of Early Indica Rice Varieties [J]. Scientia Agricultura Sinica, 40(8): 1,622-1,629.

花后高温对小麦籽粒淀粉合成及相关酶活性的影响

Effects of Post-Anthesis High Temperature Stress on Starch Synthesis and Activities of Related Enzymes in Wheat Grain

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