新疆农业科学 ›› 2021, Vol. 58 ›› Issue (10): 1929-1937.DOI: 10.6048/j.issn.1001-4330.2021.10.021
收稿日期:
2021-01-03
出版日期:
2021-10-20
发布日期:
2021-10-26
通信作者:
张一弓
作者简介:
柯梅(1986-),女,新疆巴里坤人,高级实验师,硕士,研究方向为牧草遗传与育种,(E-mail) km1108@163.com
基金资助:
Mei KE1(), Yurong HOU1, Yigong ZHANG2()
Received:
2021-01-03
Online:
2021-10-20
Published:
2021-10-26
Correspondence author:
Yigong ZHANG
Supported by:
摘要:
【目的】研究塔乌库姆冰草耐碱性机理,分析根系分泌的有机酸与pH值在调控植物抗碱性之间的关系。【方法】将2种碱性盐Na2CO3、NaHCO3按摩尔比9∶1混合,人工模拟10、30、60、90、120 mmol/L天然碱性生境条件对塔乌库姆冰草幼苗进行碱胁迫,测定pH值与有机酸种类及含量,分析其对胁迫的生理响应。【结果】碱胁迫导致塔乌库姆冰草胚根、胚芽生长缓慢,其中胚根生长率仅为对照的0.40倍,受到明显抑制;随处理液浓度的增加,幼苗根系分泌以苹果酸、柠檬酸为主的有机酸,使植物根系内pH保持在6.65~6.68。冰草根系分泌的有机酸在碱胁迫过程中虽然明显增加,但不能阻止高浓度的pH对植物幼苗的伤害。【结论】碱处理液浓度为60 mmol/L,即pH为9.8时,有机酸分泌量为调控冰草根系pH最适宜浓度。
中图分类号:
柯梅, 侯钰荣, 张一弓. 碱胁迫下冰草根系pH值与有机酸含量变化[J]. 新疆农业科学, 2021, 58(10): 1929-1937.
Mei KE, Yurong HOU, Yigong ZHANG. Study on the Relationship between pH and Organic Acid in the Root of Agropyron cristatum Tawukumu under Alkaline Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(10): 1929-1937.
分类 Classification | 浓度 Concentration (mmol/L) | 电导率 Electric conductivity (μS/cm) | 水势 Water potential (MPa) | pH值 | 相对含水量 Relative water content (%) |
---|---|---|---|---|---|
对照(CK) | 0 | 2.17 | -0.05 | 6.10 | 87.82 |
碱处理 Alkali treatment | 10 | 3.23 | -0.14 | 9.17 | 86.02 |
30 | 4.85 | -0.23 | 9.46 | 85.65 | |
60 | 5.38 | -0.30 | 9.76 | 84.75 | |
90 | 6.25 | -0.35 | 9.92 | 83.80 | |
120 | 7.14 | -0.39 | 10.00 | 82.24 | |
150 | 8.01 | -0.51 | 10.08 | 80.82 |
表1 处理液基本信息
Table 1 Basic information table of processing liquid
分类 Classification | 浓度 Concentration (mmol/L) | 电导率 Electric conductivity (μS/cm) | 水势 Water potential (MPa) | pH值 | 相对含水量 Relative water content (%) |
---|---|---|---|---|---|
对照(CK) | 0 | 2.17 | -0.05 | 6.10 | 87.82 |
碱处理 Alkali treatment | 10 | 3.23 | -0.14 | 9.17 | 86.02 |
30 | 4.85 | -0.23 | 9.46 | 85.65 | |
60 | 5.38 | -0.30 | 9.76 | 84.75 | |
90 | 6.25 | -0.35 | 9.92 | 83.80 | |
120 | 7.14 | -0.39 | 10.00 | 82.24 | |
150 | 8.01 | -0.51 | 10.08 | 80.82 |
名称 Tag | 浓度 Concentration(mmol/L) | 处理前 Treatment front 根系外pH值 Root environment pH | 处理后 After treatment | |
---|---|---|---|---|
根系外pH值 Root environment pH | 根系组织pH值 Root tissue pH | |||
对照(CK) | 0 | 6.097±0.032f | 6.497±0.020d | 6.610±0.017b |
碱处理 Alkali treatment | 10 | 9.167±0.038e | 8.487±0.097c | 6.647±0.009ab |
30 | 9.463±0.084d | 8.580±0.042c | 6.667±0.020ab | |
60 | 9.757±0.038c | 8.960±0.036b | 6.662±0.029ab | |
90 | 9.917±0.027b | 9.007±0.033b | 6.680±0.023a | |
120 | 10.000±0.015ab | 9.093±0.018ab | 6.653±0.009ab | |
150 | 10.077±0.023a | 9.160±0.025a | 6.669±0.010ab |
表2 根系pH值变化方差
Table 2 Variance analysis table of root pH variation
名称 Tag | 浓度 Concentration(mmol/L) | 处理前 Treatment front 根系外pH值 Root environment pH | 处理后 After treatment | |
---|---|---|---|---|
根系外pH值 Root environment pH | 根系组织pH值 Root tissue pH | |||
对照(CK) | 0 | 6.097±0.032f | 6.497±0.020d | 6.610±0.017b |
碱处理 Alkali treatment | 10 | 9.167±0.038e | 8.487±0.097c | 6.647±0.009ab |
30 | 9.463±0.084d | 8.580±0.042c | 6.667±0.020ab | |
60 | 9.757±0.038c | 8.960±0.036b | 6.662±0.029ab | |
90 | 9.917±0.027b | 9.007±0.033b | 6.680±0.023a | |
120 | 10.000±0.015ab | 9.093±0.018ab | 6.653±0.009ab | |
150 | 10.077±0.023a | 9.160±0.025a | 6.669±0.010ab |
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