新疆农业科学 ›› 2023, Vol. 60 ›› Issue (10): 2532-2540.DOI: 10.6048/j.issn.1001-4330.2023.10.023
左筱筱1(), 颜安1(), 宁松瑞2, 杨利1, 孙萌1, 卢前成1
收稿日期:
2023-01-03
出版日期:
2023-10-20
发布日期:
2023-11-01
通信作者:
颜安(1983-),男,四川资阳人,教授,博士,硕士生导师,研究方向为农业资源与环境,(E-mail)作者简介:
左筱筱(1998-),女,四川内江人,硕士研究生,研究方向为土壤改良与培肥,(E-mail)zuoxiaoxiao710@163.com
基金资助:
ZUO Xiaoxiao1(), YAN An1(), NING Songrui2, YANG Li1, SUN Meng1, LU Qiancheng1
Received:
2023-01-03
Online:
2023-10-20
Published:
2023-11-01
Correspondence author:
YAN An (1983 -), Male, from Ziyang, Sichuan;Ph.D.supervisor, Mainly engaged in agricultural resources and environmental research,(E-mail)Supported by:
摘要:
【目的】 研究不同生物有机肥对盐碱胁迫春小麦生长和土壤改良培肥的效果,为盐碱地的生物有机肥施用提供理论基础。【方法】 采用盆栽试验,设置9个处理:不施肥处理(CK),4种不同菌种及配比的生物有机肥(A、B、C、D),每种生物有机肥设2个施量水平(1 125,2 250 kg/hm2)。【结果】 与CK相比,施用生物有机肥后春小麦LAI、SPAD、理论产量、土壤有机质和速效养分含量等均显著增加(P<0.05),其中春小麦理论产量比CK处理增加了41.85%~74.93%,0~10 cm土层土壤有机质、碱解氮、速效磷和速效钾含量分别比CK提高了19.57%~66.24%、20.73%~40.12%、37.70%~75.72%和11.98%~31.12%,10~20 cm土层土壤有机质、碱解氮、速效磷和速效钾含量分别比CK提高了19.13%~74.63%、17.38%~29.93%、21.76%~59.52%和11.98%~31.12%,且随着生物有机肥施量的增加呈上升趋势;各施肥处理与不施肥CK相比显著降低了土壤pH和电导率(P<0.05),0~10 cm土层土壤pH、电导率分别比CK降低了2.55%~5.02%、10.33%~40.51%,10~20 cm土层土壤pH、电导率分别比CK降低了1.69%~4.20%、21.3%~43.60%。各施肥处理春小麦根际土壤细菌和放线菌数量显著高于CK(P<0.05),真菌数量显著低于CK(P<0.05),根际土壤细菌和放线菌数量随生物有机肥施量的增加呈上升趋势,真菌数量呈现下降趋势。【结论】 D生物有机肥(沼泽红假单胞菌∶肉桂褐链霉菌∶胶冻样芽孢杆菌∶枯草芽孢杆菌=1∶2∶2∶2)施用量为2 250 kg/hm2时,对盐碱土壤改良培肥和调节微生物数量结构作用效果较好,且促进了小麦生产量的提高。
中图分类号:
左筱筱, 颜安, 宁松瑞, 杨利, 孙萌, 卢前成. 盐碱麦田生物有机肥促生增产培肥效果[J]. 新疆农业科学, 2023, 60(10): 2532-2540.
ZUO Xiaoxiao, YAN An, NING Songrui, YANG Li, SUN Meng, LU Qiancheng. Study on the effect of Bio-Organic fertilizer on promoting growth and increasing yield in saline alkali wheat field[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2532-2540.
处理 Treatments | 施量1 Application rate 1 (1 125 kg/hm2) | 施量2 Application rate 2 (2 250 kg/hm2) |
---|---|---|
CK | / | / |
A | A1 | A2 |
B | B1 | B2 |
C | C1 | C2 |
D | D1 | D2 |
表1 施肥处理设计
Tab.1 Fertilization treatment design
处理 Treatments | 施量1 Application rate 1 (1 125 kg/hm2) | 施量2 Application rate 2 (2 250 kg/hm2) |
---|---|---|
CK | / | / |
A | A1 | A2 |
B | B1 | B2 |
C | C1 | C2 |
D | D1 | D2 |
图1 施加不同生物有机肥下春小麦LAI变化 注:不同小写字母表示各处理间有显著差异(P<0.05),下同
Fig.1 Effects of different bio organic fertilizers on LAI value of Spring Wheat Note: different lowercase letters indicate significant differences among treatments (P < 0.05),the same as below
处理 Treatments | 穗长 Spike length (cm) | 穗数 Panicle number (104 /hm2) | 穗粒数 Grains per panicle (粒) | 千粒重 1000 grain weight (g) | 理论产量 Grain yield (kg/hm2) |
---|---|---|---|---|---|
CK | 8.03±0.10c | 487.28±18.99a | 18.30±0.58b | 31.33±1.00c | 2 969.33±111.25c |
A1 | 9.22±0.59b | 509.85±13.71a | 26.70±4.16a | 37.95±1.94ab | 4 541.14±299.60ab |
A2 | 9.75±0.24ab | 528.25±67.84a | 28.70±3.06a | 38.31±2.79ab | 4 870.87±172.79ab |
B1 | 9.11±0.15b | 510.93±48.23a | 24.00±1.00a | 35.93±2.00b | 4 212.25±214.39b |
B2 | 9.19±0.31b | 518.33±19.13a | 28.00±2.83a | 37.86±1.50ab | 4 565.46±234.36ab |
C1 | 9.22±0.21b | 559.15±72.48a | 24.30±1.53a | 36.10±1.43b | 4 517.22±380.55ab |
C2 | 9.55±0.02ab | 546.76±45.78a | 27.50±2.12a | 38.13±4.42ab | 4 798.89±514.37ab |
D1 | 9.51±0.14ab | 580.61±6.69a | 27.00±7.07a | 38.59±2.30ab | 4 774.79±232.06ab |
D2 | 9.84±0.10a | 541.05±26.29a | 28.00±2.83a | 40.14±2.88a | 5 194.13±224.64a |
表2 春小麦产量及其构成因素
Tab.2 Spring wheat yield and its components
处理 Treatments | 穗长 Spike length (cm) | 穗数 Panicle number (104 /hm2) | 穗粒数 Grains per panicle (粒) | 千粒重 1000 grain weight (g) | 理论产量 Grain yield (kg/hm2) |
---|---|---|---|---|---|
CK | 8.03±0.10c | 487.28±18.99a | 18.30±0.58b | 31.33±1.00c | 2 969.33±111.25c |
A1 | 9.22±0.59b | 509.85±13.71a | 26.70±4.16a | 37.95±1.94ab | 4 541.14±299.60ab |
A2 | 9.75±0.24ab | 528.25±67.84a | 28.70±3.06a | 38.31±2.79ab | 4 870.87±172.79ab |
B1 | 9.11±0.15b | 510.93±48.23a | 24.00±1.00a | 35.93±2.00b | 4 212.25±214.39b |
B2 | 9.19±0.31b | 518.33±19.13a | 28.00±2.83a | 37.86±1.50ab | 4 565.46±234.36ab |
C1 | 9.22±0.21b | 559.15±72.48a | 24.30±1.53a | 36.10±1.43b | 4 517.22±380.55ab |
C2 | 9.55±0.02ab | 546.76±45.78a | 27.50±2.12a | 38.13±4.42ab | 4 798.89±514.37ab |
D1 | 9.51±0.14ab | 580.61±6.69a | 27.00±7.07a | 38.59±2.30ab | 4 774.79±232.06ab |
D2 | 9.84±0.10a | 541.05±26.29a | 28.00±2.83a | 40.14±2.88a | 5 194.13±224.64a |
图4 施加不同生物有机肥下土壤有机质、碱解氮、速效磷、速效钾含量变化
Fig.4 Effects of applying different bio organic fertilizers on the contents of soil organic matter, alkali hydrolyzable nitrogen, available phosphorus and available potassium
处理 Treat- ments | 细菌 Bacteria ( 106 cfu/g) | 真菌 Fungus ( 104 cfu/g) | 放线菌 Actinomycetes ( 105 cfu/g) |
---|---|---|---|
CK | 1.15±0.07d | 4.95±0.49a | 2.40±0.14e |
A1 | 2.85±0.07bc | 3.35±1.48b | 5.25±0.49bc |
A2 | 3.55±0.01b | 1.45±0.49cde | 5.55±0.91b |
B1 | 2.30±0.04c | 0.76±0.21e | 3.75±0.49d |
B2 | 2.70±0.06bc | 0.61±0.21e | 4.80±0.28bcd |
C1 | 2.35±0.01c | 0.93±0.01de | 4.20±1.13cd |
C2 | 3.25±0.08bc | 0.89±0.01e | 4.80±0.28bcd |
D1 | 3.40±0.04b | 2.65±0.77bc | 5.35±0.21bc |
D2 | 5.150±0.06a | 2.30±0.14bcd | 7.00±0.42a |
表3 不同生物有机肥下土壤微生物数量变化
Tab.3 Effects of different bio organic fertilizers on the number of soil microorganisms
处理 Treat- ments | 细菌 Bacteria ( 106 cfu/g) | 真菌 Fungus ( 104 cfu/g) | 放线菌 Actinomycetes ( 105 cfu/g) |
---|---|---|---|
CK | 1.15±0.07d | 4.95±0.49a | 2.40±0.14e |
A1 | 2.85±0.07bc | 3.35±1.48b | 5.25±0.49bc |
A2 | 3.55±0.01b | 1.45±0.49cde | 5.55±0.91b |
B1 | 2.30±0.04c | 0.76±0.21e | 3.75±0.49d |
B2 | 2.70±0.06bc | 0.61±0.21e | 4.80±0.28bcd |
C1 | 2.35±0.01c | 0.93±0.01de | 4.20±1.13cd |
C2 | 3.25±0.08bc | 0.89±0.01e | 4.80±0.28bcd |
D1 | 3.40±0.04b | 2.65±0.77bc | 5.35±0.21bc |
D2 | 5.150±0.06a | 2.30±0.14bcd | 7.00±0.42a |
项目 Projects | 特征值 Characteristic value | 方差贡献率 Variance contribution rate (%) | 累积方差 贡献率 Cumulative variance contribution rate (%) |
---|---|---|---|
主成分1 Principal component 1 | 15.957 | 88.649 | 88.649 |
主成分2 Principal component 2 | 1.004 | 5.575 | 94.224 |
表4 主成分的特征值与方差贡献率
Tab.4 eigenvalues and variance contribution rate of principal component analysis
项目 Projects | 特征值 Characteristic value | 方差贡献率 Variance contribution rate (%) | 累积方差 贡献率 Cumulative variance contribution rate (%) |
---|---|---|---|
主成分1 Principal component 1 | 15.957 | 88.649 | 88.649 |
主成分2 Principal component 2 | 1.004 | 5.575 | 94.224 |
项目 Projects | 主成分1 Principal component 1 | 主成分2 Principal component 2 | ||
---|---|---|---|---|
载荷值 Load value | 特征向量 Feature vector | 载荷值 Load value | 特征向量 Feature vector | |
LAI(X1) | 0.946 | 0.237 | -0.133 | -0.133 |
SPAD(X2) | 0.946 | 0.237 | -0.104 | -0.104 |
产量(X3) | 0.978 | 0.245 | -0.127 | -0.126 |
0~10 cm pH(X4) | -0.987 | -0.247 | -0.067 | -0.067 |
10~20 cm pH(X5) | -0.962 | -0.241 | -0.160 | -0.160 |
0~10 cm电导率(X6) | -0.966 | -0.242 | 0.109 | 0.108 |
10~20 cm电导率(X7) | -0.973 | -0.244 | -0.070 | -0.069 |
0~10 cm有机质(X8) | 0.938 | 0.235 | 0.127 | 0.126 |
10~20 cm有机质(X9) | 0.894 | 0.224 | 0.077 | 0.077 |
0~10 cm碱解氮(X10) | 0.985 | 0.246 | -0.083 | -0.083 |
10~20 cm碱解氮(X11) | 0.967 | 0.242 | -0.144 | -0.144 |
0~10 cm速效钾(X12) | 0.981 | 0.246 | -0.054 | -0.054 |
10~20 cm速效钾(X13) | 0.956 | 0.239 | 0.122 | 0.122 |
0~10 cm速效磷(X15) | 0.985 | 0.246 | 0.022 | 0.022 |
10~20 cm速效磷(X16) | 0.970 | 0.243 | 0.023 | 0.023 |
细菌(X17) | 0.943 | 0.236 | 0.294 | 0.293 |
真菌(X18) | -0.514 | -0.129 | 0.832 | 0.831 |
放线菌(X19) | 0.954 | 0.239 | 0.254 | 0.253 |
表5 主成分分析的载荷值与特征向量
Tab.5 load value and eigenvector of principal component analysis
项目 Projects | 主成分1 Principal component 1 | 主成分2 Principal component 2 | ||
---|---|---|---|---|
载荷值 Load value | 特征向量 Feature vector | 载荷值 Load value | 特征向量 Feature vector | |
LAI(X1) | 0.946 | 0.237 | -0.133 | -0.133 |
SPAD(X2) | 0.946 | 0.237 | -0.104 | -0.104 |
产量(X3) | 0.978 | 0.245 | -0.127 | -0.126 |
0~10 cm pH(X4) | -0.987 | -0.247 | -0.067 | -0.067 |
10~20 cm pH(X5) | -0.962 | -0.241 | -0.160 | -0.160 |
0~10 cm电导率(X6) | -0.966 | -0.242 | 0.109 | 0.108 |
10~20 cm电导率(X7) | -0.973 | -0.244 | -0.070 | -0.069 |
0~10 cm有机质(X8) | 0.938 | 0.235 | 0.127 | 0.126 |
10~20 cm有机质(X9) | 0.894 | 0.224 | 0.077 | 0.077 |
0~10 cm碱解氮(X10) | 0.985 | 0.246 | -0.083 | -0.083 |
10~20 cm碱解氮(X11) | 0.967 | 0.242 | -0.144 | -0.144 |
0~10 cm速效钾(X12) | 0.981 | 0.246 | -0.054 | -0.054 |
10~20 cm速效钾(X13) | 0.956 | 0.239 | 0.122 | 0.122 |
0~10 cm速效磷(X15) | 0.985 | 0.246 | 0.022 | 0.022 |
10~20 cm速效磷(X16) | 0.970 | 0.243 | 0.023 | 0.023 |
细菌(X17) | 0.943 | 0.236 | 0.294 | 0.293 |
真菌(X18) | -0.514 | -0.129 | 0.832 | 0.831 |
放线菌(X19) | 0.954 | 0.239 | 0.254 | 0.253 |
图5 不同生物有机肥促生增产培肥效果综合得分
Fig.5 comprehensive score of theeffect of different bio organic fertilizers on promoting growth, increasing yield and fertilizing
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