棉花陆海渐渗杂交群体纤维品质性状的QTL定位

doi:10.6048/j.issn.1001-4330.2023.01.002

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (1): 11-16.DOI: 10.6048/j.issn.1001-4330.2023.01.002

• 作物遗传育种 · 耕作栽培 · 种质资源 · 生理生化 • 上一篇下一篇

棉花陆海渐渗杂交群体纤维品质性状的QTL定位

马君¹^,²(), 杨延龙¹, 师维军¹, 汪鹏龙², 郑巨云¹, 郭仁松¹, 胡文冉³, 杨洋³()

1.新疆农业科学院经济作物研究所, 乌鲁木齐 830091
2.新疆农业科学院库车陆地棉试验站,新疆库车 842000
3.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091

收稿日期:2022-05-16 出版日期:2023-01-20 发布日期:2023-03-07
通信作者: 杨洋 (1983-),男,甘肃陇西人,副研究员,博士,研究方向为棉花株型分子育种,(E-mail)yangyang8361@163.com
作者简介:马君(1983-),男,甘肃武威人,副研究员,研究方向为棉花种质资源及陆地棉新品种选育,(E-mail)xj.majun@163.com
基金资助:
新疆维吾尔自治区重大专项“机采棉新品种培育及技术示范”(2021A02001-4);新疆农业科学院青年基金项目 “陆海渐渗杂交群体的纤维品质性状QTL定位”(xjnkq-2018002)

Analysis of QTL Mapping for Fiber Quality Traits in Introgression Lines Cotton

MA Jun¹^,²(), YANG Yanlong¹, SHI Weijun¹, WANG Penglong², ZHENG Juyun¹, GUO Rensong¹, HU Wenran³, YANG Yang³()

1. Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Xinjiang Academy of Agricultural Sciences Kuqa County Upland Cotton Test Station,Xinjiang Academy of Agricultural Sciences,Kuqa Xinjiang 842000,China
3. Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091,China

Received:2022-05-16 Online:2023-01-20 Published:2023-03-07
Correspondence author: YANG Yang (1983-),male, associate researcher, born in Longxi, Gansu province, doctor. Research area: molecular breeding for cotton plant architecture,(E-mail)yangyang8361@163.com
Supported by:
Xinjiang Uygur Autonomous Region "Cultivation and Technology Demonstration of New Varieties of Machine-picked Cotton"(2021A02001-4);The major special project of Youth Fund Project of Xinjiang Academy of Agricultural Sciences“QTL Mapping of Fiber Quality Traits in a Introgression Iines Hybrid Population”(xjnkq-2018002)

摘要/Abstract

摘要：

【目的】有效发掘利用海岛棉优异性状基因,拓宽陆地棉栽培种遗传基础。【方法】采用新疆主栽早中熟陆地棉品种新陆中60号为母本,与优质海岛棉品种新海41号为父本杂交,并以新陆中60号为轮回亲本构建出由151个BC₁F₁单株组成的回交群体,利用SSR分子标记和Join Map4.0软件构建遗传连锁图谱,采用复合区间作图法(CIM)对BC₁F₂纤维品质性状的进行QTL定位。【结果】构建的遗传连锁图谱包含52个多态性标记、14个连锁群,该图谱总长824 cM,覆盖棉花基因组的18.5%;最长的连锁群为150.3 cM,包含6个标记,最短的为0.3 cM,包含2个标记。检测到1个与纤维上半部平均长度相关的QTL,qFL-Chr14-1,定位在第14号染色体上,解释表型变异8.59%。【结论】筛选的与优质QTL位点相关SSR标记可应用于棉花优质分子标记辅助选择。

关键词: 棉花; 陆海渐渗系; 纤维品质; SSR 标记; QTL定位

Abstract:

【Objective】 The research laid a foundation for the function research of high quality gene of long-staple cotton and broad genetic basis of upland cotton. 【Methods】 In this study, Xinjiang’s main early-middle-maturing upland cotton variety Xinluzhong 60 was used as the female parent and the high-quality island cotton variety Xinhai 41 was used as the male parent. Then, the BC₁F₂ population composed of 151 individual plants combined with Xinluzhong 60 as the recurrent parent was used as the mapping population to construct a genetic linkage map. We excavated stable QTLs related to fiber quality to provide a basis for molecular marker-assisted selection breeding. The linkage map was constructed with SSR markers by Join Map 4.0 software. The QTLs for fiber quality properties in BC₁F₂ populations were mapped by the inclusive composite interval mapping method with Ici Mapping. 【Results】 The map included 52 SSR loci and 14 linkage groups, covering 824 cM and accounting for 18.5% of the cotton genome. And the longest linkage group is 150.3 cM, containing 6 markers, and the shortest is 0.3 cM, containing 2 markers.A QTL related to the average length of the upper half of the fiber was detected, located on chromosome 14. It can be used as a candidate gene for functional research of cotton fiber quality genes in land-sea introgression lines. 【Conclusion】 The polymorphic markers selected in this study can assist early breeding selection.

Key words: cotton; land-sea progressive in filtration; fiber quality; SSR marker; QTL mapping

中图分类号:

S562

马君, 杨延龙, 师维军, 汪鹏龙, 郑巨云, 郭仁松, 胡文冉, 杨洋. 棉花陆海渐渗杂交群体纤维品质性状的QTL定位[J]. 新疆农业科学, 2023, 60(1): 11-16.

MA Jun, YANG Yanlong, SHI Weijun, WANG Penglong, ZHENG Juyun, GUO Rensong, HU Wenran, YANG Yang. Analysis of QTL Mapping for Fiber Quality Traits in Introgression Lines Cotton[J]. Xinjiang Agricultural Sciences, 2023, 60(1): 11-16.

图/表 3

参考文献 24

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性状 Traits	亲本Parent
性状 Traits	父本 Male parent	母本 Female parent	均值 Mean	方差 Variance	峰度 Kurtosis	偏度 Skewness	最小值 Maximum	最大值 Maximum
纤维长度 Fiber length (mm)	39.87	30.15	30.88	1.64	-0.025	-0.001	35.79	25.98
纤维强度 Fiber strength (cN/tex)	50.00	30.92	35.52	2.63	0.020	0.417	41.30	29.00
马克隆值 Micronaire	4.60	4.42	3.89	0.52	0.204	-0.297	5.22	2.39
纤维整齐度 Fiber uniformity (%)	94.32	85.63	85.44	1.70	-0.028	-0.098	90.50	79.90
伸长率 Elongation(%)	8.75	8.28	9.84	1.46	1.630	1.034	15.90	7.10

性状 Traits	亲本Parent
性状 Traits	父本 Male parent	母本 Female parent	均值 Mean	方差 Variance	峰度 Kurtosis	偏度 Skewness	最小值 Maximum	最大值 Maximum
纤维长度 Fiber length (mm)	39.87	30.15	30.88	1.64	-0.025	-0.001	35.79	25.98
纤维强度 Fiber strength (cN/tex)	50.00	30.92	35.52	2.63	0.020	0.417	41.30	29.00
马克隆值 Micronaire	4.60	4.42	3.89	0.52	0.204	-0.297	5.22	2.39
纤维整齐度 Fiber uniformity (%)	94.32	85.63	85.44	1.70	-0.028	-0.098	90.50	79.90
伸长率 Elongation(%)	8.75	8.28	9.84	1.46	1.630	1.034	15.90	7.10

性状 Traits	纤维长度 Fiber length (mm)	断裂比强度 Fiber strength (cN/tex)	马克隆值 Micronaire	纤维整齐度 Fiber uniformity (%)	伸长率 Elongation (%)
纤维长度 Fiber length(mm)	1
断裂比强度 Fiber strength (cN/tex)	0.068	1
马克隆值 Micronaire	-0.534^**	-0.008	1
纤维整齐度 Fiber uniformity (%)	0.522^**	0.872^**	-0.125	1
伸长率 Elongation (%)	0.117	0.983^**	0.116	0.908^**	1

性状 Traits	纤维长度 Fiber length (mm)	断裂比强度 Fiber strength (cN/tex)	马克隆值 Micronaire	纤维整齐度 Fiber uniformity (%)	伸长率 Elongation (%)
纤维长度 Fiber length(mm)	1
断裂比强度 Fiber strength (cN/tex)	0.068	1
马克隆值 Micronaire	-0.534^**	-0.008	1
纤维整齐度 Fiber uniformity (%)	0.522^**	0.872^**	-0.125	1
伸长率 Elongation (%)	0.117	0.983^**	0.116	0.908^**	1

棉花陆海渐渗杂交群体纤维品质性状的QTL定位

Analysis of QTL Mapping for Fiber Quality Traits in Introgression Lines Cotton

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