新疆农业科学 ›› 2022, Vol. 59 ›› Issue (9): 2276-2287.DOI: 10.6048/j.issn.1001-4330.2022.09.023
• 畜牧兽医·农业装备工程与机械化·农业经济 • 上一篇 下一篇
李选文1(), 熊智2, 王金华1, 周艺萍1, 熊忠平3()
收稿日期:
2021-12-11
出版日期:
2022-09-20
发布日期:
2023-01-16
通信作者:
熊忠平
作者简介:
李选文(1995-),男,云南楚雄人,硕士研究生,研究方向为森林病虫害,(E-mail)1466215105@qq.com
基金资助:
LI Xuanwen1(), XIONG Zhi2, WANG Jinhua1, ZHOU Yiping1, XIONG Zhongping3()
Received:
2021-12-11
Online:
2022-09-20
Published:
2023-01-16
Correspondence author:
XIONG Zhongping
Supported by:
摘要:
【目的】 研究野外采集的思茅松毛虫(Dendrolimu kikuchii)成虫的肠道细菌多样性,为思茅松毛虫的防治和云南松(Pinus yunnanensis)等树木的保护奠定基础。【方法】 分别提取思茅松毛虫雄蛾和雌蛾的肠道总DNA,基于Illumina NovaSeq(PE250)平台运用高通量技术对肠道细菌16S rDNA基因V3~V4区进行测序和分析,分析雄虫和雌虫的肠道菌群的结构多样性。【结果】 从思茅松毛虫成虫中共获得1 278个操作分类单元(OTUs),涵盖28个门75个纲173个目296个科550个属。在门水平上,雄蛾和雌蛾的优势菌为变形菌门(Proteobacteria,雌81.27%/雄81.17%);在科水平上,雄蛾的优势菌科是鞘脂单胞菌科(Sphingomonadaceae,49.16%)和丛毛单胞菌科(Comamonadaceae,19.09%),雌蛾的优势菌科是欧文氏菌科(Erwiniaceae,34.09%)和丛毛单胞菌科(Comamonadaceae,16.68%);在属水平上,雄蛾的优势菌属是鞘氨醇单胞菌属(Sphingomonas,48.09%),雌蛾的优势菌属是泛生菌属(Pantoea,31.58%)。思茅松毛虫雌蛾肠道细菌的物种多样性比雄蛾丰富。雄蛾肠道中丰度差异显著性最高的菌群为α-变形菌纲(Alphaproteobacteria),雌蛾肠道中丰度差异显著性最高的菌群为γ-变形杆菌纲(Gammaproteobacteria)。【结论】 思茅松毛虫成虫肠道具有丰富的细菌资源。
中图分类号:
李选文, 熊智, 王金华, 周艺萍, 熊忠平. 思茅松毛虫成虫肠道细菌多样性[J]. 新疆农业科学, 2022, 59(9): 2276-2287.
LI Xuanwen, XIONG Zhi, WANG Jinhua, ZHOU Yiping, XIONG Zhongping. Study on the Diversity of Gut Bacteria from Adults of Dendrolimus kikuchii[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2276-2287.
样本 Sample | 原始序列数 Number of raw reads (条) | 有效序列数 Number of valid reads (条) | OUT数 Number of OTUs (个) | 不同分类阶元分类单元数(个) Number of different taxonomic categories | |||||
---|---|---|---|---|---|---|---|---|---|
门 Phylum | 纲 Class | 目 Order | 科 Family | 属 Genus | |||||
雄蛾 Male moths | CX1 | 130 311 | 128 634 | 288 | 15 | 27 | 77 | 117 | 180 |
CX2 | 129 994 | 128 057 | 501 | 23 | 46 | 105 | 176 | 273 | |
CX3 | 134 192 | 132 357 | 262 | 16 | 23 | 61 | 94 | 156 | |
Total | 394 497 | 389 048 | 724 | 24 | 53 | 131 | 218 | 366 | |
雌蛾 Femalemoths | CC1 | 284 173 | 276 887 | 701 | 24 | 52 | 121 | 202 | 359 |
CC2 | 275 621 | 269 167 | 229 | 14 | 19 | 50 | 83 | 138 | |
CC3 | 243 167 | 237 303 | 545 | 23 | 45 | 113 | 185 | 309 | |
Total | 802 961 | 783 357 | 994 | 26 | 60 | 147 | 248 | 460 | |
Total | 1 197 458 | 1 172 405 | 1 278 | 28 | 75 | 173 | 296 | 550 |
表 1 思茅松毛虫成虫肠道细菌测序的基本信息
Table 1 Sequencing information gut bacteria in adults of Dendrolimus kikuchii
样本 Sample | 原始序列数 Number of raw reads (条) | 有效序列数 Number of valid reads (条) | OUT数 Number of OTUs (个) | 不同分类阶元分类单元数(个) Number of different taxonomic categories | |||||
---|---|---|---|---|---|---|---|---|---|
门 Phylum | 纲 Class | 目 Order | 科 Family | 属 Genus | |||||
雄蛾 Male moths | CX1 | 130 311 | 128 634 | 288 | 15 | 27 | 77 | 117 | 180 |
CX2 | 129 994 | 128 057 | 501 | 23 | 46 | 105 | 176 | 273 | |
CX3 | 134 192 | 132 357 | 262 | 16 | 23 | 61 | 94 | 156 | |
Total | 394 497 | 389 048 | 724 | 24 | 53 | 131 | 218 | 366 | |
雌蛾 Femalemoths | CC1 | 284 173 | 276 887 | 701 | 24 | 52 | 121 | 202 | 359 |
CC2 | 275 621 | 269 167 | 229 | 14 | 19 | 50 | 83 | 138 | |
CC3 | 243 167 | 237 303 | 545 | 23 | 45 | 113 | 185 | 309 | |
Total | 802 961 | 783 357 | 994 | 26 | 60 | 147 | 248 | 460 | |
Total | 1 197 458 | 1 172 405 | 1 278 | 28 | 75 | 173 | 296 | 550 |
门Phylum | 科 Family | 属 Genus | |||
---|---|---|---|---|---|
雌成虫 Female adult | 雄成虫 Male adult | 雌成虫 Female adult | 雄成虫 Male adult | 雌成虫 Female adult | 雄成虫 Male adult |
变形菌门 Proteobacteria (88.27%) | 变形菌门 Proteobacteria (88.17%) | 欧文氏菌科 Erwiniaceae (34.09%) | 鞘脂单胞菌科 Sphingomonadaceae (49.16%) | 泛生菌属 Pantoea (31.58%) | 鞘氨醇单胞菌属 Sphingomonas (48.90%) |
Actinobacteriota(7.20%) | Bacteroidota (5.19%) | 丛毛单胞菌科 Comamonadaceae(16.68%) | 丛毛单胞菌科Comamonadaceae (19.09%) | 鞘氨醇单胞菌属Sphingomonas (10.03%) | 水瓶菌属 Aquabacteriaum (13.27%) |
厚壁菌门 Firmicutes (5.57%) | 厚壁菌门 Firmicutes (3.02%) | 鞘脂单胞菌Sphingomonadaceae (10.19%) | 柄杆菌科Caulobacteraceae (8.76%) | 假单胞菌 Pelomonas (7.71%) | 短波单胞菌属 Brevundimonas (5.06%) |
Bacteroidota (3.58%) | Actinobacteriota (1.65%) | 弧菌科 Vibrionaceae (7.08%) | 黄杆菌科Flavobacteriaceae (3.43%) | 弧菌属 Vibrio (7.08%) | 黄杆菌 Flavobacterium (3.42%) |
丙酸杆菌科Propionibacteriaceae (3.56%) | 莫拉氏菌科Moraxellaceae (2.42%) | 水瓶菌属 Aquabacteriaum (6.40%) | 不粘柄菌属 Asticcacaulis (3.21%) | ||
伯克氏菌科Burkholderiaceae (3.14%) | 伯克氏菌科Burkholderiaceae (1.24%) | Cutibacterium (3.53%) | 丛毛单胞菌科未鉴定到属unclassified_f__Comam- onadaceae(2.69%) | ||
柄杆菌科Caulobacteraceae (3.01%) | 红环菌科Rhodocyclaceae (1.22%) | 青枯菌属 Ralstonia (2.70%) | 不动杆菌属 Acinetobacter (2.38%) | ||
肠杆菌科Enterobacteriaceae (2.08%) | 草酸杆菌科Oxalobacteraceae (1.02%) | 欧文氏菌属Erwinia (2.51%) | 假单胞菌 Pelomonas (1.80%) | ||
微杆菌科Microbacteriaceae (1.36%) | 毛螺菌科Lachnospiraceae (0.56%) | 短波单胞菌属Brevundimonas (2.25%) | Methyloversatilis (1.20%) | ||
黄杆菌科Flavobacteriaceae (1.35%) | 乳酸细菌科Lactobacillaceae (0.51%) | 微囊藻毒素降解菌 Paucibacter (1.59%) | 微囊藻毒素降解菌 Paucibacter (1.05%) | ||
毛螺菌科Lachnospiraceae (1.23%) | 肠杆菌科Enterobacteriaceae (0.31%) | 黄杆菌 Flavobacterium (1.35%) | 乳酸菌属 Lactobacillus (0.51%) | ||
乳酸杆菌科 Lactobacillaceae (1.10%) | 丙酸杆菌科Propionibacteriaceae (0.18%) | Kosakonia (1.23%) | Cutibacterium (0.18%) | ||
莫拉氏菌科 Moraxellaceae (1.09%) | 弧菌科 Vibrionaceae (0.15%) | 乳酸菌属 Lactobacillus (1.10%) | 弧菌属 Vibrio (0.15%) | ||
红环菌科 Rhodocyclaceae (0.23%) | 欧文氏菌科Erwiniaceae (0.13%) | 不动杆菌属 Acinetobacter (1.00%) | 泛生菌属 Pantoea (0.13%) | ||
微杆菌科Microbacteriaceae (0.10%) | 丛毛单胞菌科未鉴定到属unclassified_f__ Comamonadaceae (0.74%) | ||||
不粘柄菌属Asticcacaulis (0.60%) | |||||
Methyloversatilis (0.22%) |
表 2 思茅松毛虫雌雄成虫体内在门、科和属分类等级上相对丰度
Table 2 Relative abundance at the levels of phylum, family and genus in female and male adults of Dendrolimus kikuchii(≥0.1%)
门Phylum | 科 Family | 属 Genus | |||
---|---|---|---|---|---|
雌成虫 Female adult | 雄成虫 Male adult | 雌成虫 Female adult | 雄成虫 Male adult | 雌成虫 Female adult | 雄成虫 Male adult |
变形菌门 Proteobacteria (88.27%) | 变形菌门 Proteobacteria (88.17%) | 欧文氏菌科 Erwiniaceae (34.09%) | 鞘脂单胞菌科 Sphingomonadaceae (49.16%) | 泛生菌属 Pantoea (31.58%) | 鞘氨醇单胞菌属 Sphingomonas (48.90%) |
Actinobacteriota(7.20%) | Bacteroidota (5.19%) | 丛毛单胞菌科 Comamonadaceae(16.68%) | 丛毛单胞菌科Comamonadaceae (19.09%) | 鞘氨醇单胞菌属Sphingomonas (10.03%) | 水瓶菌属 Aquabacteriaum (13.27%) |
厚壁菌门 Firmicutes (5.57%) | 厚壁菌门 Firmicutes (3.02%) | 鞘脂单胞菌Sphingomonadaceae (10.19%) | 柄杆菌科Caulobacteraceae (8.76%) | 假单胞菌 Pelomonas (7.71%) | 短波单胞菌属 Brevundimonas (5.06%) |
Bacteroidota (3.58%) | Actinobacteriota (1.65%) | 弧菌科 Vibrionaceae (7.08%) | 黄杆菌科Flavobacteriaceae (3.43%) | 弧菌属 Vibrio (7.08%) | 黄杆菌 Flavobacterium (3.42%) |
丙酸杆菌科Propionibacteriaceae (3.56%) | 莫拉氏菌科Moraxellaceae (2.42%) | 水瓶菌属 Aquabacteriaum (6.40%) | 不粘柄菌属 Asticcacaulis (3.21%) | ||
伯克氏菌科Burkholderiaceae (3.14%) | 伯克氏菌科Burkholderiaceae (1.24%) | Cutibacterium (3.53%) | 丛毛单胞菌科未鉴定到属unclassified_f__Comam- onadaceae(2.69%) | ||
柄杆菌科Caulobacteraceae (3.01%) | 红环菌科Rhodocyclaceae (1.22%) | 青枯菌属 Ralstonia (2.70%) | 不动杆菌属 Acinetobacter (2.38%) | ||
肠杆菌科Enterobacteriaceae (2.08%) | 草酸杆菌科Oxalobacteraceae (1.02%) | 欧文氏菌属Erwinia (2.51%) | 假单胞菌 Pelomonas (1.80%) | ||
微杆菌科Microbacteriaceae (1.36%) | 毛螺菌科Lachnospiraceae (0.56%) | 短波单胞菌属Brevundimonas (2.25%) | Methyloversatilis (1.20%) | ||
黄杆菌科Flavobacteriaceae (1.35%) | 乳酸细菌科Lactobacillaceae (0.51%) | 微囊藻毒素降解菌 Paucibacter (1.59%) | 微囊藻毒素降解菌 Paucibacter (1.05%) | ||
毛螺菌科Lachnospiraceae (1.23%) | 肠杆菌科Enterobacteriaceae (0.31%) | 黄杆菌 Flavobacterium (1.35%) | 乳酸菌属 Lactobacillus (0.51%) | ||
乳酸杆菌科 Lactobacillaceae (1.10%) | 丙酸杆菌科Propionibacteriaceae (0.18%) | Kosakonia (1.23%) | Cutibacterium (0.18%) | ||
莫拉氏菌科 Moraxellaceae (1.09%) | 弧菌科 Vibrionaceae (0.15%) | 乳酸菌属 Lactobacillus (1.10%) | 弧菌属 Vibrio (0.15%) | ||
红环菌科 Rhodocyclaceae (0.23%) | 欧文氏菌科Erwiniaceae (0.13%) | 不动杆菌属 Acinetobacter (1.00%) | 泛生菌属 Pantoea (0.13%) | ||
微杆菌科Microbacteriaceae (0.10%) | 丛毛单胞菌科未鉴定到属unclassified_f__ Comamonadaceae (0.74%) | ||||
不粘柄菌属Asticcacaulis (0.60%) | |||||
Methyloversatilis (0.22%) |
样本Sample | Shannon指数 Shannon index | Simpson指数 Simpson index | ACE指数 ACE index | Chao1指数 Chao1 index | Coverage指数 Coverage index | |
---|---|---|---|---|---|---|
雌蛾 Female moths | CC1 | 4.315 7 | 0.053 9 | 716.411 7 | 712.812 5 | 0.999 9 |
CC2 | 1.311 8 | 0.380 4 | 499.567 4 | 380.500 0 | 0.999 6 | |
CC3 | 2.671 4 | 0.147 4 | 613.331 5 | 635.617 6 | 0.999 7 | |
均值±标准差 Mean ± SD | 2.766 3±1.504 2a | 0.193 9±0.168 2a | 609.770 2±108.47a | 576.310 0±173.91a | 0.999 7 | |
雄蛾 Male moths | CX1 | 2.498 3 | 0.255 4 | 323.119 9 | 333.000 0 | 0.999 7 |
CX2 | 2.990 1 | 0.213 0 | 530.314 9 | 573.769 2 | 0.999 6 | |
CX3 | 2.262 1 | 0.294 9 | 290.810 4 | 299.187 5 | 0.999 7 | |
均值±标准差 Mean ± SD | 2.583 5±0.371 4a | 0.254 4±0.040 9a | 381.415 1±129.96a | 401.985 6±149.73a | 0.999 7 |
表 3 思茅松毛虫雌雄成虫体内在门、科和属分类等级上相对丰度
Table 3 Relative abundance at the levels of phylum, family and genus in female and male adults of Dendrolimus kikuchii
样本Sample | Shannon指数 Shannon index | Simpson指数 Simpson index | ACE指数 ACE index | Chao1指数 Chao1 index | Coverage指数 Coverage index | |
---|---|---|---|---|---|---|
雌蛾 Female moths | CC1 | 4.315 7 | 0.053 9 | 716.411 7 | 712.812 5 | 0.999 9 |
CC2 | 1.311 8 | 0.380 4 | 499.567 4 | 380.500 0 | 0.999 6 | |
CC3 | 2.671 4 | 0.147 4 | 613.331 5 | 635.617 6 | 0.999 7 | |
均值±标准差 Mean ± SD | 2.766 3±1.504 2a | 0.193 9±0.168 2a | 609.770 2±108.47a | 576.310 0±173.91a | 0.999 7 | |
雄蛾 Male moths | CX1 | 2.498 3 | 0.255 4 | 323.119 9 | 333.000 0 | 0.999 7 |
CX2 | 2.990 1 | 0.213 0 | 530.314 9 | 573.769 2 | 0.999 6 | |
CX3 | 2.262 1 | 0.294 9 | 290.810 4 | 299.187 5 | 0.999 7 | |
均值±标准差 Mean ± SD | 2.583 5±0.371 4a | 0.254 4±0.040 9a | 381.415 1±129.96a | 401.985 6±149.73a | 0.999 7 |
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