多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术

doi:10.6048/j.issn.1001-4330.2020.04.007

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (4): 636-641.DOI: 10.6048/j.issn.1001-4330.2020.04.007

多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术

王冬梅, 李彩虹, 刘建

新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091

收稿日期:2019-11-04 发布日期:2020-03-26
作者简介:王冬梅(1969-),女,新疆人,研究员,硕士,研究方向为转基因棉安全评价,(E-mail)wdm872@ sina.com
基金资助:
国家重点研发计划(2017YFD0201900)

Study of Molecular Analysis of the Gut Contents of Hippodamia variegata as a Method for Detecting Intraguild Predation of Chrysoperla nipponensis

WANG Dongmei, LI Caihong, LIU Jian

Key Laboratory of Intergraded Pest Management of Crops in China North-Western Oasis, Ministry of Agriculture and Rural Affairs, P. R. China / Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2019-11-04 Published:2020-03-26
Correspondence author: Wang Dongmei(1969-),Female, Professor, Master, focus on safety assessment of transgenic cotton, (E-mail)wdm872@ sina.com
Supported by:
Supported by the National Key R & D Program Project (2017YFD0201900)

摘要/Abstract

摘要： 【目的】研究利用分子检测技术评价棉田多异瓢虫对日本通草蛉的集团内捕食作用。【方法】根据基因库 NCBI 中的日本通草蛉的线粒体细胞色素氧化酶 I(COI)的基因序列(登录号为 KY806757),设计日本通草蛉的特异引物,并测定特异引物的灵敏度。利用 DNA 检测技术,在室内以该引物检测喂食5粒日本通草蛉卵的多异瓢虫成虫中肠内靶标食物的衰变情况,估算多异瓢虫对日本通草蛉的半衰期。【结果】所设计引物只对日本通草蛉DNA具有扩增效果,对与其同域发生的其它害虫和天敌不具扩增作用,其扩增片段大小约为220 bp,其最低检出浓度为0.117 ng/μL。多异瓢虫成虫喂食日本通草蛉卵后,检出率随消化时间呈现逐渐下降的趋势,到16 h检出率为0,多异瓢虫成虫半衰期为8.32 h。【结论】利用该技术可定量评价多异瓢虫与日本通草蛉集团内竞争。

关键词: 日本通草蛉; 细胞色素氧化酶 I 基因; 多异瓢虫成虫; 中肠内容物; 集团内竞争

Abstract: 【Objective】 To study on intraguild predation by adult Hippodamia variegata (Coleoptera:Coccinellidae) on Chrysoperla nipponensis by using molecular gut content analysis.【Method】 Molecular analysis were used to detect the DNA of C. nipponensis in the gut contents of H. variegate. Primers for the C. nipponensis were designed according to cytochrome oxidase subunit I gene in Genbank (accession No.KY806757), and the sensitivity of this primer was tested. Moreover, feeding experiments were performed to determine how long prey DNA remained detectable in the guts of H. variegate.【Result】 Species-specific tests showed that the primers were only used to amplify the DNA of the lacewing in C. nipponensis, and had no effect on other pests and natural enemies occurring in the The specificity tests performed with the primers showed that the single band presented in C. nipponensis, and no amplification to other pest and natural enemy of same ecological system. Primer The band was about 220 bp. The sensitivity tests detected with the primers indicat ed that 0.117 ng/μL of prey DNA can could be detected. Feeding experiments showed the detectability of prey DNA declined with digestion time extended. The half-life for adult H. variegata was estimated to be 8.32 h.【Conclusion】 This method can be used to study quantificationally the IGP by H. variegata on C. nipponensis predators in the field.

Key words: Chrysoperla nipponensis; Cytochrome oxidase subunit I gene; adult Hippodamia variegata; gut content analysis; intraguild predation

中图分类号:

S562

王冬梅, 李彩虹, 刘建. 多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术[J]. 新疆农业科学, 2020, 57(4): 636-641.

WANG Dongmei, LI Caihong, LIU Jian. Study of Molecular Analysis of the Gut Contents of Hippodamia variegata as a Method for Detecting Intraguild Predation of Chrysoperla nipponensis[J]. Xinjiang Agricultural Sciences, 2020, 57(4): 636-641.

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多异瓢虫成虫对日本通草蛉集团内捕食作用的分子检测技术

Study of Molecular Analysis of the Gut Contents of Hippodamia variegata as a Method for Detecting Intraguild Predation of Chrysoperla nipponensis

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