水稻OsHOX6启动子的组织特异性表达

doi:10.6048/j.issn.1001-4330.2019.10.005

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (10): 1800-1805.DOI: 10.6048/j.issn.1001-4330.2019.10.005

• 作物遗传育种·种质资源·分子生物学 • 上一篇下一篇

水稻OsHOX6启动子的组织特异性表达

何丹¹, 玉山江·麦麦提², 杨渡², 韩盛², Inez Hortense Slamet-Loedin³

1.新疆维吾尔自治区林业技术推广总站,乌鲁木齐 830000;
2.农业部西北荒漠绿洲作物有害生物综合治理重点实验室新疆农业科学院植物保护研究所,乌鲁木齐 830091;
3.国际水稻研究所作物育种和生物化学系转基因研究室, 菲律宾

收稿日期:2019-07-12 发布日期:2019-11-22
通信作者: 玉山江·麦麦提(1978-),男,新疆人,副研究员,博士,研究方向为农作物抗病抗逆机理,(E-mail)yusanjan418@163.com
作者简介:何丹(1989-),女,四川人,助理工程师,硕士,研究方向为植物生理,(E-mail)10780365098@qq.com
基金资助:
自治区公益性科研院所基本科研业务费专项(KY2014036)

Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice

HE Dan¹, Yushanjiang Maimaiti², YANG Du², HAN Shen², Slamet-Loedin I.H.³

1.Forestry Technology Extension General Station of Xinjiang Uygur Autonomous Region, Urumqi 830000, China;
2.Key Laboratory of Intergraded Management of Harmful Crop Vermin in China North-Western Oasis, Ministry of Agriculture, P. R. China/Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
3.Gene Transformation Lab, Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Laguna, Philippines

Received:2019-07-12 Published:2019-11-22
Correspondence author: Yushanjiang Maimaiti(1978-) ,Male,Born in Xinjiang ,Doctor, Associate researcher, The research direction is study on the mechanism of disease resistance and stress resistance of crops,(E-mail) yusanjan418@163.com

摘要/Abstract

摘要： 【目的】研究水稻HD-ZipⅠ转录因子家族的成员OsHOX6基因启动子的表达。【方法】通过构建水稻OsHOX6基因启动子与GUS基因融合表达载体,利用农杆菌(Agrobacterium)介导,以未成熟水稻胚作为试验材料,转化到水稻IR64,通过PCR检测和潮霉素抗性筛选出阳性的转基因植株,从不同组织取样品,进行X-Gluc染色并观察。【结果】转基因植株的叶、根、茎、花等器官经过X-Gluc染色后,主要在侧根、花粉以及组织损伤部分出现蓝色斑点,其它组织均未检测出蓝色斑点,观察根解剖结构,绿色斑点集中在根内皮层。【结论】 水稻OsHOX6基因启动子能够驱动GUS基因,在转基因水稻侧根和花粉上特异表达。

关键词: 水稻; OsHOX6基因; 特异性表达; 侧根和花粉; 转基因

Abstract: 【Objective】 To study the expression of OsHOX6 gene promoter, a member of homeodomain-leucine zipper (HD-Zip I) transcription factor family in rice (Oryza sativa L.) 【Method】 The plant expression vector with fusion gene, OsHOX6 promoter and GUS gene was established, and IR64 was transformed by the agro bacterium-mediated transformation method, then roots, stems, leaves and pollen and other organs in transformed plants during in the different growing stage were dyed with X-Gluc. 【Result】 The transgenic plants with hygromysine resistance and positive detection by PCR were obtained. It showed that some blue spots in the lateral roots, pollen and wound tissue in the plants with fusion gene (OsHOX6 promoter and GUS gene) were detected by using X-Gluc dye, while other organs did not display blue spots, It was found that the green spots were concentrated in the root cortex, indicating that the rice OsHOX6 gene promoter could drive the GUS gene and express it specifically on the transgenic rice lateral roots and pollen. 【Conclusion】 The promoter is specifically expressed in rice lateral roots and pollen, which will be further studied in the study of genes related to lateral roots and endocortical formation in rice.

Key words: rice; gene OsHOX6; specific expression; lateral roots; transgenic

中图分类号:

:S511

何丹, 玉山江·麦麦提, 杨渡, 韩盛, Inez Hortense Slamet-Loedin. 水稻OsHOX6启动子的组织特异性表达[J]. 新疆农业科学, 2019, 56(10): 1800-1805.

HE Dan, Yushanjiang Maimaiti, YANG Du, HAN Shen, Slamet-Loedin I.H.. Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice[J]. Xinjiang Agricultural Sciences, 2019, 56(10): 1800-1805.

参考文献

[1] Kashiwagi, J. , Krishnamurthy, L. , Crouch, J. H. , & Serraj, R.Variability of root length density and its contributions to seed yield in chickpea (cicer arietinum l.) under terminal drought stress. Field Crops Research, 2006,95(2-3):171-181.
[2] José R Dinneny. A gateway with a guard: how the endodermis regulates growth through hormone signaling. Plant Science, 2014, 214(1):14-19.
[3] 刘大同, 荆彦平, 李栋梁,等.植物侧根发育的研究进展[J].植物生理学报,2013, 49 (11): 1 127-1 137.   LIU Da-tong, JING Yan-ping, LI Dong-lilang, et al. Research Advances in Plant Lateral Root Development [J]. Plant Physiology Communications, 2013,49 (11): 1,127-1,137.
[4] Yang, J. , Zhang, J. , Huang, Z. , Wang, Z. , Zhu, Q. , & Liu, L.Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. ann bot. Ann Bot, 2002,90(3):369-377.
[5] Pauluzzi, G. , Divol, F. , Puig, J. , Guiderdoni, E. , Dievart, A. , & C. Périn. Surfing along the root ground tissue gene network. Developmental Biology, 2012, 365(1):14-22.
[6] 王金祥,严正龙,潘瑞炽.不定根形成与植物激素的关系[J].植物生理学通讯,2005,(1):133-142.   WANG Jin-xiang, YAN Zhen-long, PAN Rui-chi. Research Advances in Plant Lateral Root Development [J]. Plant Physiology Communications, 2005,(1):133-142.
[7] Kawata, S. and Harada, J. On the Root Development of the Rice Plants(Oryza Sativa L.) in Different Varieties Cultivated at the Saline Paddy Field . Proc. Crop Sci. Soc. Jpn, 1975,45:438-457. (in Japanese)
[8] Coudert, Y. , Christophe Périn, Courtois, B. , Khong, N. G. , & Gantet, P. Genetic control of root development in rice, the model cereal. Trends in Plant Science, 2010,15(4):219-226.
[9] Gan, S. , & Amasino, R. M. Inhibition of leaf senescence by autoregulated production of cytokinin. Science (Washington D C), 1995,270(5244):1,986-1,988.
[10] Smigocki, A. C. , & Owens, L. D. .Cytokinin gene fused with a strong promoter enhances shoot organogenesis and zeatin levels in transformed plant cells. Proceedings of the National Academy of Sciences, 1988, 85(14):5,131-5,135.
[11] Cabello, J. V. , Dezar, C. A. , Manavella, P. A. , & Chan, R. L. The intron of thearabidopsis thaliana cox5c gene is able to improve the drought tolerance conferred by the sunflowerhahb-4 transcription factor. Planta(Berlin), 2007, 226(5):1,143-1,154.
[12] Dai, M. , Hu, Y. , Ma, Q. , Zhao, Y. , & Zhou, D. X. Functional analysis of ricehomeobox4(oshox4) gene reveals a negative function in gibberellin responses. Plant Molecular Biology, 2008,66(3):289-301.
[13] Agalou, A., Sigit Purwantomo, vern s E,, Henrik Johannesson, Xiaoyi Zhu, & Amy Estiati, et al. A genome-wide survey of hd-zip genes in rice and analysis of drought-responsive family members. Plant Molecular Biology, 2008, 66(1-2):87-103.
[14] 王宏, 李刚波, 张大勇, 等. 植物HD-Zip 转录因子的生物学功能[J].遗传,2013, 35(10): 1 179-1 188.   WANG Hong, LI Gang-bo, ZHANG Da-yong, et al.Biological functions of HD-Zip transcription factors [J]. Hereditas, 2013,35(10): 1,179-1,188.
[15] Hiei, Y., Komari, T., & Kubo, T. Transformation of rice mediated by agrobacterium tumefaciens. Plant Molecular Biology, 1997,35(1-2):205-218.

水稻OsHOX6启动子的组织特异性表达

Studies on Tissue Specific Regulation of OsHOX6 Promoter in Rice

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张燕红, 侯天钰, 巴音花, 赵彩月, 吕玉平, 布哈丽且木·阿不力孜, 赵志强, 李冬, 杜孝敬, 袁杰, 王奉斌. 水稻重组自交系群体芽期和苗期耐盐性鉴定与评价[J]. 新疆农业科学, 2023, 60(5): 1041-1049.
[2]	胡文冉, 赵准, 邵武奎, 黄全生. 陆地棉TCP家族基因鉴定及组织表达分析[J]. 新疆农业科学, 2023, 60(11): 2627-2637.
[3]	布哈丽且木·阿不力孜, 张燕红, 袁杰, 赵志强, 文孝荣, 杜孝敬, 王奉斌, 吕玉平, 阿曼古丽·艾孜子. 新疆优质丰产香型水稻品种筛选与评价[J]. 新疆农业科学, 2023, 60(11): 2694-2703.
[4]	陈丽, 马静, 朱志明, 刘炜, 孙建昌. 基于水稻RIL群体的加工品质性状QTL分析[J]. 新疆农业科学, 2023, 60(10): 2419-2425.
[5]	蒲胜海, 王则玉, 丁峰, 王彩风, 刘小利, 马晓鹏, 王涛, 彭银双, 李韵同. 膜下滴灌水稻生理生化特性对灌浆期控水的响应[J]. 新疆农业科学, 2022, 59(9): 2091-2103.
[6]	杜孝敬, 张燕红, 吕玉平, 袁杰, 李冬, 赵志强, 布哈丽且木·阿不力孜, 王奉斌. 不同香稻品种种子萌发和苗期对NaCl胁迫的响应[J]. 新疆农业科学, 2022, 59(4): 827-838.
[7]	杜月梅, 高丽萍, 邵华. 呋虫胺及其代谢物UF和DN在水稻上残留行为及其储存稳定性[J]. 新疆农业科学, 2022, 59(3): 663-673.
[8]	王振洋, 王冀川, 袁杰, 王奉斌. 不同施氮量与栽插密度对水稻群体生长及产量构成的影响[J]. 新疆农业科学, 2022, 59(12): 2969-2978.
[9]	王露霞, 索常凯, 褚璇, 冶军, 闵伟, 侯振安. 旱稻和水稻生长发育及产量对灌水量的响应[J]. 新疆农业科学, 2022, 59(12): 2979-2987.
[10]	张燕红, 杜孝敬, 康民泰, 文孝荣, 唐福森, 袁杰, 赵志强, 布哈丽且木·阿不力孜, 虢虎, 王奉斌. 不同盐浓度下外源微生物菌对水稻生长发育及产量的影响[J]. 新疆农业科学, 2022, 59(12): 2988-2996.
[11]	丁峰, 蒲胜海, 吕玉平, 马晓鹏, 沈晓明, 莫彦. 不同灌溉定额对膜下滴灌水稻耗水特征及水分生产效率的影响[J]. 新疆农业科学, 2021, 58(9): 1577-1584.
[12]	韩雪琴, 沈文娟, 张振海, 田雷, 罗成科, 杨淑琴, 李培富, 张银霞. 水稻稻瘟病抗性基因在抗性育种中的研究进展[J]. 新疆农业科学, 2021, 58(3): 483-492.
[13]	吴璇, 伏红伟, 王琳丽, 苏宏华, 杨益众. 棉蚜茧蜂对不同品种、不同生育期棉花的趋性行为反应[J]. 新疆农业科学, 2020, 57(6): 1136-1141.
[14]	布哈丽且木·阿不力孜, 袁杰, 朱小霞, 张燕红, 陈长青, 赵志强, 贾春平, 文孝荣, 康民泰, 唐福森, 王奉斌. 新疆稻区优质丰产水稻品种筛选与评价[J]. 新疆农业科学, 2020, 57(11): 2108-2117.
[15]	曾晓燕, 赵瑞海, 李志博, 魏亦农. 棉花PsbS基因对烟草光合特性的影响[J]. 新疆农业科学, 2019, 56(4): 589-598.