新疆农业科学 ›› 2019, Vol. 56 ›› Issue (5): 910-918.DOI: 10.6048/j.issn.1001-4330.2019.05.014

• 土壤肥料·贮藏加工·植物保护·畜牧水产 • 上一篇    下一篇

基于PCR检测的4种土传病害病原菌侵染棉花的动态分析

许泰百1, 萨吉达木·艾则孜1, 吴琼1, 李玉华1, 李进2, 郭庆元1   

  1. 1.新疆农业大学农学院,乌鲁木齐 830052;
    2.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091
  • 收稿日期:2019-04-15 发布日期:2019-05-20
  • 通信作者: 郭庆元(1962-),男,四川人,教授,博士生导师,研究方向为植物病理学,(E-mail)guoqingyuan3009@sina.com
  • 作者简介:许泰百(1992-),女,山东人,硕士研究生,研究方向为作物病害防治,(E-mail)2909920967@qq.com
  • 基金资助:
    国家重点研发计划“棉花化肥农药减施技术集成研究与示范”(2017YFD0201903);公益性行业(农业)科研专项“棉花苗期根腐类病害综合治理技术”(201503112-6);新疆农业大学研究生科研创新项目“4种棉花苗期病害相互关系影响”研究(XJAUGRI2016-013)

Dynamic Analysis of Four Species of Soil-Borne Disease Pathogens on Cotton Based on PCR Detection

XU Tai-bai1, Sajidamu Aizezi1, WU Qiong1, LI Yu-hua1, LI Jin 2, GUO Qing-yuan1   

  1. 1. College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Research Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2019-04-15 Published:2019-05-20
  • Correspondence author: GUO Qing-yuan( 1962-), Male, Sichuan people,Professor ,(E-mail)guoqingyuan3009@sina.com
  • Supported by:
    Supported by the National Key R & D Plan Project "Integration and Demonstration of Pesticide and Fertilizer Reduction Technology " (2017YFD0201903); Special Fund for Public Welfare Industry (Agriculture) Scientific R & D Program of Ministry of Agriculture, P. R. China "Integrated Treatment Technology for Root Rot Disease in Cotton Seedling Stage (201503112-6); The Graduate Research Innovation Project of Xinjiang Agricultural University "Study on the Relationship between the Four Kinds of Cotton Seedling Diseases" (XJAUGRI2016-013)

摘要: 【目的】立枯病、红腐病、枯萎病和黄萎病是新疆棉花上的4种主要土传病害,研究4种病害的病原菌在新疆棉花上的侵染动态,分析各自的侵染始期和最佳防治时期,为病害高效防控提供依据。【方法】针对4种主要病害的病原菌,对混合接菌后分期采集的棉株进行特异性引物PCR检测,依据检测结果,进行各病原菌侵染动态分析及混合侵染分析。【结果】4种病原菌从棉花子叶期即能侵染;立枯丝核菌侵染相对较早、较快,拟轮枝镰孢霉次之;2种病原菌均在棉苗前期有较高的侵染株率,3叶期达到高峰,此后侵染株率逐渐降低;而尖孢镰孢霉和大丽轮枝菌,虽从子叶期开始即可侵染,但侵染株率较低,直到蕾铃期侵染株率呈渐增趋势;病原菌之间的混合侵染普遍存在,以两菌混合侵染居多。【结论】子叶期为4种病原菌的侵染初期;立枯丝核菌和拟轮枝镰孢霉以苗期侵染为特点;尖孢镰孢霉和大丽轮枝菌以子叶期到蕾铃期均可侵染为特点;病原菌之间常有混合侵染发生。

关键词: 棉花; 土传病害; 立枯丝核菌; 拟轮枝镰孢霉; 尖孢镰孢霉; 大丽轮枝菌; 侵染动态

Abstract: 【Objective】 Rhizoctonia damping off, Fusarium red rot, Fusarium wilt and Verticillium wilt are four major soil-borne diseases on cotton in Xinjiang. This project aims to clarify the infection dynamics of the four diseases of cotton in Xinjiang and analyze the initial infection period and the best control period of each disease in the hope of providing a basis for the high efficiency prevention and control of the disease. 【Methods】In this study, specific primer PCR was used to detect the pathogen of four main diseases, and the dynamic analysis and mixed infection analysis of each pathogen were carried out according to the detection results.【Results】The results indicated that the four pathogens could be infested from the cotyledon stage of cotton; the infection of Rhizoctonia solani was detected relatively earlier and faster, followed by Fusarium verticillioides, the infection rate of the two pathogens was high in the early stage of cotton seedling and reached the peak at the three-leaf stage, and the detected rate decreased gradually after that. Although the Fusarium oxysporum and Verticillium dahliae could also be infected from the cotyledon stage, but the rate of detection in the cotyledon to the one-leaf stage was low, Until the time of the boll stage invasion plant rate was gradually increasing. The mixed infection between the pathogens was widely spread, and the mixed infection of the two species pathogens was the most common.【Conclusions】Cotyledon stage is the infection start period of four species pathogens. R. solani and F. verticillioides is characterized by seedling invasion, while F.oxysporum and V.dahliae is characterized by invasion from cotyledon stage to boll stage; There are often mixed infections among pathogens

Key words: cotton; soil-borne diseases; Rhizoctonia solani; Fusarium verticillioides; Fusarium oxysporum; Verticillium dahliae; infection dynamics

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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