棉铃红粉病鉴定及气候条件对病菌生长和孢子萌发的影响

doi:10.6048/j.issn.1001-4330.2018.10.009

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (10): 1837-1846.DOI: 10.6048/j.issn.1001-4330.2018.10.009

棉铃红粉病鉴定及气候条件对病菌生长和孢子萌发的影响

焦瑞莲¹,任毓忠¹,李志强²,董海涛²,武刚³,张莉¹,李国英^1,2

1.石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室,新疆石河子 832003;
2.石河子开发区石大惠农科技开发有限公司,新疆石河子 832003;
3.新疆生产建设兵团第一师农科所,新疆阿拉尔 843300

发布日期:2019-01-24
通信作者: 张莉(1970-),女,江苏人,教授,研究方向为植物病理学,(E-mail)1602784618@qq.com
李国英(1941-),男,河北人,教授,研究方向为植物病原真菌与真菌病害,(E-mail)lgyshu@163.com
作者简介:焦瑞莲(1989-),女,安徽人,硕士研究生,研究方向为植物病理学,(E-mail) 252132051@qq.com
基金资助:
科技部中小企业创新项目;国家重点资助研发项目(2017YFD0201900)

The Identification of Pathogen of Pink Mold Rot on Cotton Boll and the Influence of Climatic Conditions on the Growth and Spore Germination of the Fungus

JIAO Rui-lian¹,REN Yu-zhong¹,LI Zhi-qiang²,DONG Hai-tao²,WU Gang³, ZGANG Li¹,LI Guo-ying^1,2

1.Key Laboratory for Oasis Agricultural Pest Management and Plant Resource Utilization at Universities of Xinjiang Uygur Autonomous Region, College of Agronomy, Shihezi University, Shihezi Xinjiang 832003, China;
2. Huinong Technology Development Co., Ltd. of Shihezi University, Shihezi Xinjiang 832003, China;
3. Agricultural Science Institute of 1st Diversion of XPCC, Aral Xinjiang 843300, China

Published:2019-01-24
Correspondence author: ZHANG Li(1970-) ,female,Professor,(E-mail) 1602784618@ qq. com
LI Guo-ying(1941-) ,male,Professor,(E-mail) lgyshu@ 163. com

摘要/Abstract

摘要： 【目的】 研究新疆棉铃红粉病致病菌,分析不同温度、湿度和光照条件对该菌生长、产孢量及孢子萌发的影响。【方法】 通过对病菌的分离培养、致病性测定、形态学观察以及病原菌rDNA-ITS序列分析进行病原鉴定。设置不同温度、湿度和光照,对该菌菌落生长、产孢量及孢子萌发进行测定。【结果】 引起新疆棉铃红粉病的病原菌为Trichothecium roseum;该菌在10~35℃均可生长,生长最适温度为25℃左右,在5℃以下及40℃以上时不能生长;完全黑暗和光暗交替有利于产孢;最适孢子萌发温度为20~25℃,小于5℃或大于40℃均不能萌发;孢子在完全黑暗、完全光照和光暗交替的条件下均可萌发,完全黑暗最适萌发;80%以上的相对湿度萌发率最高。【结论】 棉铃红粉菌的致病菌是T. roseum。温度在25℃左右、完全黑暗和光暗交替有利生长和产孢;温度为20~25℃、完全黑暗、80%以上的相对湿度有利于孢子萌发。

关键词: 棉铃红粉菌; 鉴定; 菌落生长; 产孢量; 孢子萌发

Abstract: 【Objective】 To clear and definite the effects of different temperature, humidity and light on the growth, sporulation and spore germination of cotton boll red powder disease in Xinjiang, and to understand the effects of different temperature, humidity and light conditions on the growth, sporulation and spore germination.【Method】 The pathogen was identified by isolation, culture, pathogenicity, morphological observation and rDNA-ITS sequence analysis. The fungus colony growth, sporulation capacity and spore germination were measured by setting different temperature, humidity and light. 【Result】 The results showed that the pathogen causing pink mold rot on cotton boll was Trichothecium roseum in Xinjiang. The strain could grow at 10-35℃ and the optimum temperature was about 25℃. It was found that it could not grow below 5℃ and above 40℃. Complete darkness and light dark alternation were beneficial to sporulation. The optimum temperature of spore germination was 20-25℃, but it could not germinate at below 5℃ and above 40℃. Spores could germinate under the condition of complete darkness, light and light dark alternation, but the optimum germination condition was complete darkness and the germination rate of spores was the highest in relative humidity above 80%.【Conclusion】 The pathogen causing the cotton boll pink mold rot was T. roseum in Xinjiang. Temperature about 25℃, complete darkness and light and dark alternately favor growth and sporulation, and the temperature at 20-25℃, completely dark, and the relative humidity above 80% were beneficial to spore germination.

Key words: pink mold rot on cotton boll; identification; colony growth; sporulation capacity; spore germination

焦瑞莲,任毓忠,李志强,董海涛,武刚,张莉,李国英. 棉铃红粉病鉴定及气候条件对病菌生长和孢子萌发的影响[J]. 新疆农业科学, 2018, 55(10): 1837-1846.

JIAO Rui-lian,REN Yu-zhong,LI Zhi-qiang,DONG Hai-tao,WU Gang, ZGANG Li,LI Guo-ying. The Identification of Pathogen of Pink Mold Rot on Cotton Boll and the Influence of Climatic Conditions on the Growth and Spore Germination of the Fungus[J]. Xinjiang Agricultural Sciences, 2018, 55(10): 1837-1846.

参考文献

[1] Richard, J. L., Pier, A. C., & Tiffany, L. H. (1970). Biological effects of toxic products from trichothecium roseum link. Mycopathologia Et Mycologia Applicata, 40(2): 161-170.
[2] Shamsi, S., & Sultana, R. (2008). Trichothecium roseum link - a new record of hyphomycetous fungus for bangladesh. Bangladesh Journal of Plant Taxonomy, 15(1): 77-80.
[3] Kwon, J. H., Shen, S. S., & Kim, J. (2010). Occurrence of pink mold rot of strawberry caused by trichothecium roseum in Korea. Plant Pathology Journal, 26(3): 296.
[4] Hamid, M. I., Hussain, M., Ghazanfar, M. U., Raza, M., & Liu, X. Z. (2015). Trichothecium roseum causes fruit rot of tomato, orange, and apple in pakistan. Plant Disease, 98(9):1,271.
[5] 章战华. 不同寄主来源的粉红聚端孢比较及活性研究[D].合肥:安徽农业大学硕士论文,2007.
ZHANG Zhan-hua.(2007). Comparison and activity of Trichothecium roseum from different host sources[D]. Master Dissertation. Anhui Agricultural University, Hefei. (in Chinese)
[6] 王勇,王万立,刘春艳,等.番茄红粉病致病病原的鉴定及其培养特性研究[J].华北农学报,2008,23(6):97-100.
WANG Yong, WANG Wan-li, LIU Chun-yan, et al. 2008. Studies on Identification and Cultural Characterization of Trichothecium roseum [J]. Acta Agriculturae Boreali-sinica, 23(6):97-100. (in Chinese)
[7] 潘月敏,高智谋,纪文飞,等.培养基营养条件对棉铃红粉病菌菌丝生长和孢子形成的影响[J].西北农林科技大学学报(自然科学版),2005,(S1):68-72.
PAN Yue-min, GAO Zhi-mou, JI Wen-fei, et al. (2005). Effects of nutrient conditions on mycelia growth and spore formation of boll erythrocytes [J]. Journal of Northwest A & F University (Natural Science) , (S1):68-72. (in Chinese)
[8] 黎鸿慧,王兆晓,赵贵元.棉铃红粉病在河北中南部的发生规律及防治[J].中国棉花,2011,38(8):45.
LI Hong-hui, WANG Zhao-xiao, ZHAO Gui-yuan. (2011). Occurrence regularity and control of Boll pink blush in central and southern Hebei [J]. China Cotton, 38(8):45. (in Chinese)
[9] 冯中红,郝蓉蓉,薛莉,等.菜豆红粉病菌的鉴定及其碳氮源利用能力测定[J].微生物学通报,2015,42(7):1 331-1 337.
FENG Zhong-hong, HAO Rong-rong, XUE Li, et al. (2015). Identification of Trichothecium roseum and determination of carbon and nitrogen sources utilization ability [J]. Microbiology, 42(7):1,331-1,337. (in Chinese)
[10] 杨济徽,李鹏,张龙,等.海南龙眼红粉病的病原鉴定[J].果树学报,2016,33(8):993-999.
YANG Ji-hui, LI Peng, ZHANG Long et al.(2016). Identification of the pink disease for longan fruit in Hainan Province [J]. Journal of Fruit Science, 33(8):993-999. (in Chinese)
[11] 董金皋.农业植物病理学[M]. 北京:中国农业出版社, 2007.
DONG Jin-gao.(2007). Agricultural Plant Pathology[M]. Beijing: China Agriculture Press. ( in Chinese)
[12] 张中义. 植物病原真菌学[M]. 成都:四川科学技术出版社, 2001.
ZHANG Zhong-yi. (2001). Plant pathogenic mycology[M]. Chendou: Sichuan Science and Technology Press. (in Chinese)
[13] 陆家云. 植物病原真菌学[M]. 北京:中国农业出版社, 2001.
LU Jia-yun. (2001). Plant Pathogenic Mycology[M]. Beijing: China Agriculture Press. ( in Chinese)
[14] 殷辉, 周建波, 常芳娟,等. 枣果贮藏期红粉病菌鉴定及环境因子对其分生孢子萌发的影响[J]. 植物病理学报, 2018,48(1):16-24.
YIN Hui, ZHOU Jian-bo, CHANG Fang-juan, et al. (2018). Identification of the pathogen causing pink m old rot in stored jujube fruit and effects of environmental factors on conidial germination [J]. Acta Phytopathologica Sinica, 48(1):16-24. (in Chinese)

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棉铃红粉病鉴定及气候条件对病菌生长和孢子萌发的影响

The Identification of Pathogen of Pink Mold Rot on Cotton Boll and the Influence of Climatic Conditions on the Growth and Spore Germination of the Fungus

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