Identification of salt tolerance of 197 upland cotton varieties at germination stage

doi:10.6048/j.issn.1001-4330.2024.07.002

Abstract

Abstract:

【Objective】By identifying and evaluating the salt tolerance of upland cotton germplasm resources during the germination stage, this research can select good salt-tolerance germplasms.Based on this evaluation, the indicators of salt tolerance identification can be clarified, which provides theoretical support for the breeding improvement of upland cotton.【Methods】In this study, 197 varieties of upland cotton from both domestic and international sources were used as experimental materials.Salt stress treatments were administered at sodium chloride (NaCl) concentrations of 0 (as the control), 150, and 200 mmol/L.Nine parameters, including germination potential, rate, root length, and their relative measurements, were evaluated for the control and treatment groups.To assess the salt tolerance of these cotton varieties, a membership function method was applied, utilizing a comprehensive D-value for evaluation.【Results】The results indicated that a concentration of 150 mmol/L is ideal for determining salt tolerance in upland cotton during the germination stage.The coefficient of variation in the treatment group was consistently higher compared to the control group.A significant positive correlation was observed between the comprehensive D-value and the various traits of the treated plants.The comprehensive D-value as the primary factor.Based on a thorough evaluation, 4 materials were classified as highly salt-tolerant, 39 materials as salt-tolerant, 65 materials as moderately salt-tolerant, and 89 materials as salt-sensitive.【Conclusion】The study indicated that nine metrics, such as germination potential, germination rate, root length, and their respective relative values, are effective indicators for assessing salt tolerance.Among the tested materials, forty-three, including varieties 20N705 and C6524, demonstrated notably strong salt tolerance.

Key words: cotton; germination stage; salt tolerance; membership function value; comprehensive evaluation

摘要：

【目的】鉴定评价陆地棉品种资源萌发期耐盐性,筛选耐盐性优异的种质,鉴定分析耐盐指标,为陆地棉育种改良提供理论支持。【方法】以197份国内外陆地棉品种为材料,以0(CK)、150和200 mmol/L的氯化钠(NaCl)为胁迫处理浓度,测试对照组与处理组的发芽势、发芽率、根长及相对值等9个指标,采用隶属函数法,利用综合评价D值评价197份陆地棉品种的耐盐性。【结果】150 mmol/L适合作为鉴定萌发期耐盐性的盐胁迫浓度,处理组的变异系数均高于对照组,综合评价D值与处理组各性状之间均存在显著性正相关性,综合评价D值为第一主成分因子,筛选出4份高耐材料、39份耐盐材料、65份中耐材料和89份敏盐材料。【结论】发芽势、发芽率和根长及其相对值等9个数据可作为耐盐性鉴定指标,筛选出20N705、C6524等43份耐盐性较强的材料。

关键词: 棉花, 萌发期, 耐盐性, 隶属函数值, 综合评价

CLC Number:

S512

LIU Huijie, WANG Junhao, GONG Zhaolong, LIANG Yajun, WANG Junduo, LI Xueyuan, ZHENG Juyun, WANG Jichuan. Identification of salt tolerance of 197 upland cotton varieties at germination stage[J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1574-1581.

刘慧杰, 王俊豪, 龚照龙, 梁亚军, 王俊铎, 李雪源, 郑巨云, 王冀川. 197份陆地棉品种萌发期耐盐性鉴定[J]. 新疆农业科学, 2024, 61(7): 1574-1581.

Figures/Tables 10

References 27

[1]	国家统计局. 国家统计局关于 2022 年棉花产量的公告[EB/OL].(2022-12-26)[2022-12-30]. http://www.stats.gov.cn/xxgk/sjfb/zxfb2020/202212/t20221227_1891259.html.
[2]	朱生堡, 乌尔古丽·托尔逊, 唐光木, 等. 新疆盐碱地变化及其治理措施研究进展[J]. 山东农业科学, 2023, 55(3):158-165.
	ZHU Shengbao, Wuerguli Tuoerxun, Tang Guangmu, et al. Research Progress on Saline-Alkali Land Changes and Its Treatment Measures in Xinjiang[J]. Shandong Agricultural Sciences, 2023, 55(3):158-165.
[3]	王俊铎, 黎玉华, 龚照龙, 等. 棉花耐盐性研究进展[J]. 棉花科学, 2021, 43(5):3-10.
	WANG Junduo, LI Yuhua, GONG Zhaolong, et al. Research Progresses on Salt Tolerence of Cotton[J]. Cotton Science, 2021, 43(5):3-10.
[4]	孙小芳, 郑青松, 刘友良. 盐胁迫下不同基因型棉花萌发生长和离子吸收特性[J]. 棉花学报, 2001,(3):134-137.
	SUN Xiaofang, ZHENG Qingsong, LIU Youliang. Characteristics of Growth and Ion Absorbability in Different Geno types of Cotton at Germinating and Seedling Stages under Salt Stresses[J]. Cotton Science, 2001,(3):134-137.
[5]	Zahra M, Tahira L, Sahar N, et al. An overview of salinity stress, mechanism of salinity tolerance and strategies for its management in cotton#13[J]. Frontiers in Plant Science, 2022,13
[6]	陈莹, 王萌, 王晓禹. 棉花种子耐盐碱能力快速筛选研究[J]. 中国种业, 2022,(9):64-70.
	Chen Ying, Wang Meng, Wang Xiaoyu, et al. Rapid screening study on saline-alkali tolerance of cotton seeds[J]. China's seed industry, 2022,(9):64-70.
[7]	张国伟, 路海玲, 张雷, 等. 棉花萌发期和苗期耐盐性评价及耐盐指标筛选[J]. 应用生态学报, 2011, 22(8):2045-2053.
	Zhang Guowei, Lu Hailing, Zhang Lei, etc. Salt tolerance evaluation and salt tolerance index screening of cotton at germination stage and seedling stage[J]. Application Ecology, 2011, 22(8): 2045-2053.
[8]	杜磊, 雄东毕, 李成奇, 等. 盐胁迫下黄河流域棉花品种主要性状的通径分析及优异种质筛选[J]. 江苏农业科学, 2023, 51(14):101-108.
	DU Lei, XIONG Dongbi, LI Chengqi, et al. Path Analysis of Main Traits and Superior Germplasm Screening of Cotton Cultivars in Yellow River Region Under Salt Stress[J]. Jiangsu Agricultural Sciences, 2023, 51(14):101-108.
[9]	王俊铎, 曾辉, 龚照龙, 等. 陆地棉品种资源耐复合盐碱性综合评价分析[J]. 中国农业科技导报, 2019, 21(10):1-11.
	Wang Junduo, Zeng Hui, Gong Zhaolong, et al. Comprehensive Assessment of Complex Salt-alkali Tolerance of the Upland Cotton Varities Resources[J]. Journal of Agricultural Science and Technology, 2019, 21(10):1-11.
[10]	陈雪梅, 刘骅峻, 杨曌, 等. 盐碱胁迫对苜蓿种子萌发性状的影响及耐盐碱性评价[J]. 黑龙江农业科学, 2024,(3):64-70.
	CHEN XUEmei, LIU Huajun, YANG Zhao, et al. Effects of saline-alkali stress on germination traits of alfalfa seeds and evaluation of saline-alkali tolerance[J]. Heilongjiang Agricultural Sciences, 2024,(3):64-70.
[11]	郑巨云, 曾辉, 王俊铎, 等. 陆地棉品种资源萌发期耐盐性的隶属函数法评价[J]. 新疆农业科学, 2018, 55(9):1579-1592.
	ZHENG Juyun, ZENG Hui, WANG Junduo, et al. Comprehensive Assessment of Salt Tolerance of the Upland Cotton Varities Resources in Seedling Stage by Membership Function Method[J]. Xinjiang Agricultural Sciences, 2018, 55(9):1579-1592.
[12]	姜瑛, 周萌, 吴越, 等. 不同燕麦品种耐盐性差异及其生理机制[J]. 草业科学, 2018, 35(12):2903-2914.
	JIANG Ying, ZHOU Meng, WU Yue, et al. Evaluation of Salt Tolerance and Its Underlying Physiological Mechanisms in Different Oats[J]. Pratacultural Science, 2018, 35(12):2903-2914.
[13]	徐佳陵, 曾文官, 袁延超, 等. 陆地棉种质资源萌发出苗期耐盐性鉴定[J]. 山东农业科学, 2017, 49(9):32-36.
	XU Jialing, ZENG Wenguan, YUAN Yanchao, et al. Salt Tolerance Evaluation at Germination Stage of Upland Cotton Germplasms[J]. Shandong Agricultural Sciences, 2017, 49(9):32-36.
[14]	白灯莎·买买提艾力, 李寒暝, 张少民, 等. 不同棉花品种种子萌发阶段耐盐性综合评价[J]. 新疆农业科学, 2011, 48(1):18-24.
	Baidengsha·Maimaitiaili, Li Hanming, Zhang Shaomin, et al. Assesment on the Salt Resistance of Seven Cotton Varieties (Gossypium hirsutum L.) During Germination Stage[J]. Xinjiang Agricultural Sciences, 2011, 48(1):18-24.
[15]	余如刚, 王雪茄, 王国良, 等. 紫花苜蓿品种苗期耐盐性分析及评价指标筛选[J]. 草地学报, 2022, 30(7):1781-1789.
	YU Rugang, WANG Xuejia, WANG Guoliang, et al. Analysis of Salinity-Tolerance and Screening Evaluation Indicators to Salinity-Tolerance in Medicago Sativa Seedlings[J]. Journal of Grassland Science, 2022, 30(7):1781-1789.
[16]	何进尚, 冯伟东, 刘旺清, 等. NaCl胁迫对小麦种子发芽率的影响及耐盐性分析[J]. 寒旱农业科学, 2023, 2(3):229-233.
	HE JinShang, FENG Weidong, LIU Wangqing, et al. Effects of NaCl Stress on Seed Germination Rate of Wheat Varieties and Their Salt Tolerance Evaluation[J]. Journal of Cold-Arid Agricultural Sciences, 2023, 2(3):229-233.
[17]	王朋, 郑凯, 赵杰银, 等. 陆地棉种质资源材料的耐热性评价及指标筛选[J]. 新疆农业科学, 2023, 60(9):2081-2090.
	WANG Peng, ZHENG Kai, ZHAO Jieyin, et al. Evaluation and index screening of heat resistance of Gossypium hirsutum germplasm resources[J]. Xinjiang Agricultural Sciences, 2023, 60(9):2081-2090.
[18]	李双男, 郭慧娟, 王晶, 等. 不同盐碱胁迫对棉花种子萌发的影响[J]. 种子, 2018, 37(1):38-45.
	LI Shuangnan, GUO Huijuan, WANG Jing, et al. Effects of Different Saline and stress on seed[J]. Seed, 2018, 37(1):38-45.
[19]	马洪雨. 高低耐盐性大豆幼苗根叶和黄麻幼苗根响应盐胁迫的蛋白质组学研究[D]. 南京: 南京农业大学, 2015.
	MA Hongyu. Proteomic analysis of roots and leaves from soybean seedlings and roots from kenaf seedlings with different salt tolerance under salt stress[D]. Nanjing: Nanjing Agricultural University, 2015.
[20]	宋冰梅, 姜岩, 陈鑫, 等. 新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价[J]. 新疆农业科学, 2023, 60(9):2239-2247.
	SONG Bingmei, JIANG Yan, CHEN Xin, et al. Evaluation of salt tolerance and alkali tolerance of new transgenic high-yield cotton at germination stage and seedling stage[J]. Xinjiang Agricultural Sciences, 2023, 60(9):2239-2247.
[21]	余宝宝. 新疆阿拉尔地区土壤盐渍化的发育规律及防治技术[D]. 西安: 长安大学, 2021.
	YU Baobao. Development law and prevention and control technology of soil salinization in alar region, xinjiang[D]. Chang’an University, 2021.
[22]	刘娜. 陆地棉耐盐性评价及遗传分析[D]. 乌鲁木齐: 新疆农业大学, 2019.
	LIU Na. Evaluation and genetic analysis of salt tolerance in upland cotton[D]. Urumqi: Xinjiang Agricultural University, 2019.
[23]	王宁, 冯克云, 南宏宇, 等. 甘肃河西走廊棉区棉花萌发期和苗期耐盐性鉴定与评价[J]. 干旱地区农业研究, 2018, 36(1):148-155.
	WANG Ning, FENG Keyun, NAN Hongyu, et al. Salt tolerance identification and evaluation of cotton at its germination and seedling stages in Hexi area of Gansu[J]. Agricultural Research in the Arid Areas, 2018, 36(1):148-155.
[24]	施洋, 杨华, 施庆华, 等. 海盐胁迫对棉花种子萌发的影响[J]. 现代农业科技, 2022,(15):18-22,26.
	SHI Yang, YANG Hua, SHI Qinghua, et al. Effects of Sea Salt Stress on Cotton Seed Germination[J]. Modern Agricultural Sciences and Technology, 2022,(15):18-22,26.
[25]	赵康, 杨涛, 王红刚, 等. 42个新疆海岛棉品种萌发期耐盐性评价[J]. 作物杂志, 2022,(5):27-33.
	ZHAO Kang, YANG Tao, WANG Honggang, et al. Evaluation on Salt Tolerance of 42 Sea-Island Cotton (Gossypium barbadense) Varieties in Xinjiang during Germination Period[J]. Crops, 2022,(5):27-33.
[26]	罗礽兰, 胡道武, 王静静, 等. 基于主成分分析和隶属函数的棉花种子萌发期耐涝性鉴定评价[J]. 中国棉花, 2023, 50(3):1-5.
	LUO Renglan, HU Daowu, WANG Jingjing, et al. Identification and evaluation of waterlogging tolerance of cotton seed at germination stage based on the principal component analysis and subordinate function[J]. China cotton, 2023, 50(3):1-5.
[27]	黄雅婕, 任丹, 李生梅, 等. 陆地棉苗期的耐盐碱性评价及鉴定指标筛选[J]. 中国农业科技导报, 2022, 24(5):46-55.
	HUANG Yajie, REN Dan, LI Shengmei, et al. Evaluation and Screening of Salt and Alkali Tolerance Indices of Upland Cotton Seedlings[J]. Journal of Agricultural Science and Technology, 2022, 24(5):46-55.

性状 Traits		最大值 Max	最小值 Min	均值 Average	标准差 SD	变异系数 CV(%)
对照组 Control group	发芽势GP(%)	1.350	0.233	0.820	0.181	22.098
	发芽率GR(%)	1.450	0.467	0.933	0.105	11.238
	根长RL(cm)	23.729	6.038	15.102	3.655	24.205
盐胁迫 Salt stress treatment	发芽势GP(%)	1.050	0.000	0.402	0.267	66.553
	发芽率GR(%)	1.100	0.067	0.561	0.270	48.092
	根长RL(cm)	9.167	0.800	2.797	1.903	68.042

性状 Traits		最大值 Max	最小值 Min	均值 Average	标准差 SD	变异系数 CV(%)
对照组 Control group	发芽势GP(%)	1.350	0.233	0.820	0.181	22.098
	发芽率GR(%)	1.450	0.467	0.933	0.105	11.238
	根长RL(cm)	23.729	6.038	15.102	3.655	24.205
盐胁迫 Salt stress treatment	发芽势GP(%)	1.050	0.000	0.402	0.267	66.553
	发芽率GR(%)	1.100	0.067	0.561	0.270	48.092
	根长RL(cm)	9.167	0.800	2.797	1.903	68.042

性状 Traits	最大值 Max	最小值 Min	均值 Average	标准差 SD	变异系数 CV(%)
相对发芽势 RGP(%)	1.529	0.000	0.486	0.310	63.746
相对发芽率 RGR(%)	1.174	0.067	0.597	0.278	46.609
相对根长 RRL(%)	0.743	0.049	0.184	0.119	64.278

性状 Traits	最大值 Max	最小值 Min	均值 Average	标准差 SD	变异系数 CV(%)
相对发芽势 RGP(%)	1.529	0.000	0.486	0.310	63.746
相对发芽率 RGR(%)	1.174	0.067	0.597	0.278	46.609
相对根长 RRL(%)	0.743	0.049	0.184	0.119	64.278

性状 Traits	相对发芽势 GP(%)	相对发芽率 GR(%)	相对根长 RRL(%)
相对发芽势RGP(%)	1	0.817^**	0.506^**
相对发芽率RGR(%)	0.817^**	1	0.545^**
相对根长RRL(%)	0.506^**	0.545^**	1