Effects of Rape Varietieson soil Physicochemical Properties and Microbial Diversity in Saline-Alkali Land in Xinjiang

doi:10.6048/j.issn.1001-4330.2019.02.006

Abstract

Abstract: 【Objective】 In order to screen out the most suitable rape variety for planting in the saline-alkali soil in Xinjiang, a micro-area experiment was carried out to study the effects of four rape varieties (Rape variety 1, 16-P22Wai, Hua 5 and Jinyou 158) on the microbal community structure and microbal diversity and soil physicochemical properties in saline-alkali soil.【Method】The microbal community structure and microbal diversity were analyzed by using Illumia Miseq high-throughput sequencing technology.【Result】 The results showed that 16-P22Wai could significantly reduce the pH of the soil and increase its alkali-hydrolyzable N, available P and organic matter (P<0.05). There was no significant difference in microbial community richness among soil samples, and the maximum diversity index of microbial community was 1. Proteobacteria, Actinobacteria, Acidobacteria and Chloroflexi were the dominant populations in soil of different rape varieties and the order of relative richness of Pontibacter was as follows: (from big to small) 16-P22Wai > Rape variety 1 >Jinyou158 > Hua 5; The principal component analysis showed 16-P22Wai and Hua 5 had the highest similarity between 16-P22Wai and Hua 5; There was a significant correlation between the distribution of soil bacterial community and the distribution of soil alkaline hydrolyzed N and available P. 【Conclusion】 The rape variety of 16-P22Wai is more suitable for planting in saline-alkali soil for promoting the formation of dominant flora the area.

Key words: rape; saline-alkali land; dominant flora; MiSeq sequencing; bacterial community

摘要： 【目的】研究不同油菜品种对土壤细菌群落和结构的影响并分析对土壤理化性质的影响为新疆地区盐碱地种植油菜提供理论依据。【方法】以不同油菜品种1、16-P22外、华5号和金油158为研究对象,应用Illumina MiSeq测序技术。,筛选出更适宜盐碱地种植的油菜品种。【结果】种植油菜品种16-P22外对降低盐碱地土壤pH值、提高碱解氮、速效磷、有机质含量效果最显著(P<0.05);土壤样品微生物群落丰富度间无显著性差异,微生物群落多样性指数最大为1。不同油菜品种土壤中优势菌门为Proteobacteria、Actinobacteria、Acidobacteria和Chloroflexi,Pontibacter 耐盐菌属相对丰度大小顺序为16-P22外>1 >金油158>华5号; PCA主成分分析显示细菌群落中相似性最高在16-P22外与华5号之间;土壤碱解氮和速效磷与土壤细菌群落分布显著相关。【结论】油菜品种16-P22外更适宜盐碱地上进行种植,促进盐碱土壤优势菌群的形成。

关键词: 油菜, 盐碱地, 优势菌群, MiSeq测序, 细菌群落

CLC Number:

S565.4

LIU Dong-yang, XU Jie-liang, ZHANG Feng-hua. Effects of Rape Varietieson soil Physicochemical Properties and Microbial Diversity in Saline-Alkali Land in Xinjiang[J]. Xinjiang Agricultural Sciences, 2019, 56(2): 246-257.

刘东洋, 徐接亮, 张凤华. 不同油菜品种对盐碱土壤理化性质与微生物多样性的影响[J]. 新疆农业科学, 2019, 56(2): 246-257.

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