Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower

doi:10.6048/j.issn.1001-4330.2025.05.020

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (5): 1226-1233.DOI: 10.6048/j.issn.1001-4330.2025.05.020

• Plant Protection • Previous Articles Next Articles

Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower

HU Xin¹, MA Chaoyang¹, YU Boran¹, ZHANG Chenguang¹, WANG Peng², ZHAO Sifeng¹, XI Hui¹(), ZHANG Xuekun¹()

1. Key Laboratory for Oasis Agricultural Pest Management and Plant Protection Resource Utilization / Agricultural College of Shihezi University, Shihezi Xinjiang 832003, China
2. Crop Research Institute, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi Xinjiang 832000, China

Received:2024-10-21 Online:2025-05-20 Published:2025-07-09
Correspondence author: ZHANG Xuekun
About author:XI Hui (1988- ), female, from Pucheng, Shannxi, associate professor,Ph.D., research direction: plant pathology, (E-mail) xihui@shzu.edu.cn
Supported by:
Financial Technology Program Project of XPCC(2021AB011);Young and Middle-Aged Scientific and Technological Innovation Leading Talents Project of XPCC(2018CB022)

咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用

胡欣¹, 马朝阳¹, 于博然¹, 张晨光¹, 王鹏², 赵思峰¹, 惠慧¹(), 张学坤¹()

1.石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子 832000
2.新疆农垦科学院作物研究所,新疆石河子 832000

通讯作者: 张学坤
作者简介:惠慧(1988-),女,陕西蒲城人,副教授,博士,研究方向为植物病理学,(E-mail) xihui@shzu.edu.cn
基金资助:
新疆生产建设兵团财政科技计划项目(2021AB011);新疆生产建设兵团中青年科技创新领军人才计划(2018CB022)

Abstract

Abstract:

【Objective】 Screened and selected fungicide-selenium co-formulations to reduce fungicide dosage while enhancing their control efficacy against sunflower sclerotinia caused by Sclerotinia sclerotiorum,thereby providing a novel theoretical foundation for managing this disease in Xinjlang.【Methods】 The effects of fluroxonil and benzalpyrimidin on mycelia growth, mycelia morphology, oxalic acid secretion and sclerotium production of S. sclerotiorum were measured after being combined with selenium separately, and their control efficacy on sunflower sclerotinia was verified by pot experiment.【Results】 The combination of 0.5 mg/L fluroxonil and 1 mg/L benzalpyrimidin with 30 mg/L selenium significantly inhibited the mycelium growth and the generation of sclerotium, and the mycelium morphology changed. The secretion of sclerotinic acid decreased significantly after the combination of fluroxonil and selenium. In addition, the combination of two fungicides and selenium could improve the prevention and control effect of Sclerotinia stem rot to a certain extent. 【Conclusion】 Compared with a single dose, the combination of 0.5mg/L fludioxonil, 1mg/L benzyl azoxystrobin and 30mg/L selenium effectively hinder the normal growth and development of S. sclerotiorum, and enhance the protective effect on sunflower.

Key words: sunflower; Sclerotinia sclerotiorum; fungicide; selenium; combined virulence

摘要：

【目的】筛选并获得杀菌剂与硒的复配制剂,以降低杀菌剂用量并提升其对核盘菌(Sclerotinia sclerotiorum)所致向日葵菌核病的防治效果,为新疆向日葵菌核病的防治提供理论依据。【方法】咯菌腈、苯甲·嘧菌酯分别与硒复配后,测定其对向日葵核盘菌菌丝生长和菌丝形态、草酸分泌量及菌核产生量的影响,并通过盆栽试验验证其对菌核病的防效。【结果】0.5 mg/L咯菌腈和1 mg/L苯甲·嘧菌酯分别与30 mg/L硒复配后,均显著抑制了核盘菌的菌丝生长和菌核产生,且菌丝的形态发生变异;咯菌腈能抑制核盘菌的草酸分泌量。此外,2种杀菌剂与硒复配后均在一定程度上提高了对菌核病的防病效果。【结论】0.5 mg/L咯菌腈、1 mg/L苯甲·嘧菌酯与30 mg/L硒复配与单剂相较,有效阻碍了核盘菌的正常生长发育,增强向日葵的保护作用。

关键词: 向日葵, 核盘菌, 杀菌剂, 硒, 联合毒力

CLC Number:

S435.655

HU Xin, MA Chaoyang, YU Boran, ZHANG Chenguang, WANG Peng, ZHAO Sifeng, XI Hui, ZHANG Xuekun. Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1226-1233.

胡欣, 马朝阳, 于博然, 张晨光, 王鹏, 赵思峰, 惠慧, 张学坤. 咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用[J]. 新疆农业科学, 2025, 62(5): 1226-1233.

Figures/Tables 5

Fig.1 Changes of different treatments on mycelial growth rate of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

Fig.2 Changes of different treatments on mycelial morphologic variation of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

Fig. 3 Standard curve of oxalic acid (A) and oxalic acid secretion of S. sclerotiorum under different treatments (B)

Fig. 4 Changes of different treatments on the sclerotium yield of S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

Fig. 5 Determination of inhibition effect of different treatments on S. sclerotiorum Notes:A: CK; B: 0.5 mg/L fludioxonil; C: 1 mg/L benzovindiflupyr·azoxystrobin; D: 30 mg/L selenium; E: 0.5 mg/L fludioxonil + 30 mg/L selenium; F: 1 mg/L benzovindiflupyr·azoxystrobin + 30 mg/L selenium

References 26

[1]	张海洋, 李海燕, 孟庆林, 等. 啶酰菌胺对向日葵核盘菌生物活性的影响[J]. 农学学报, 2021, 11(1): 71-74, 90. DOI
	ZHANG Haiyang, LI Haiyan, MENG Qinglin, et al. Effect of biological activity of boscalid on Sclerotinia sclerotiorum of sunflower[J]. Journal of Agriculture, 2021, 11(1): 71-74, 90. DOI
[2]	Chen Z Y, Sun H Y, Hu T, et al. Sunflower resistance against Sclerotinia sclerotiorum is potentiated by selenium through regulation of redox homeostasis and hormones signaling pathways[J]. Environmental Science and Pollution Research International, 2022, 29(25): 38097-38109.
[3]	Newman T E, Kim H, Khentry Y, et al. The broad host range pathogen Sclerotinia sclerotiorum produces multiple effector proteins that induce host cell death intracellularly[J]. Molecular Plant Pathology, 2023, 24(8): 866-881. DOI PMID
[4]	羊国根, 程家森. 核盘菌致病机理研究进展[J]. 生物技术通报, 2018, 34(4): 9-15. DOI
	YANG Guogen, CHENG Jiasen. Research advances in pathogenesis of Sclerotinia sclerotiorum[J]. Biotechnology Bulletin, 2018, 34(4): 9-15. DOI
[5]	Zhao H Z, Zhou T, Xie J T, et al. Mycoparasitism illuminated by genome and transcriptome sequencing of Coniothyrium minitans, an important biocontrol fungus of the plant pathogen Sclerotinia sclerotiorum[J]. Microbial Genomics, 2020, 6(3): e000345.
[6]	Montalvão S C L, Marques E, Martins I, et al. Suppression of the phytopathogens Sclerotinia sclerotiorum and Sclerotium rolfsii by Trichoderma spp[J]. Biologia, 2023, 78(10): 2941-2952.
[7]	陈玉蓉, 曹秋林, 廉华, 等. 向日葵菌核病拮抗木霉菌筛选及防病效果[J]. 中国生物防治学报, 2022, 38(4): 846-851. DOI
	CHEN Yurong, CAO Qiulin, LIAN Hua, et al. Screening of Trichoderma and their efficiency against sunflower Sclerotinia rot[J]. Chinese Journal of Biological Control, 2022, 38(4): 846-851. DOI
[8]	Zhang H X, Xie J T, Fu Y P, et al. A 2-kb mycovirus converts a pathogenic fungus into a beneficial endophyte for Brassica protection and yield enhancement[J]. Molecular Plant, 2020, 13(10): 1420-1433.
[9]	张海洋, 李海燕, 孟庆林, 等. 不同杀菌剂对向日葵菌核病的田间防治效果[J]. 作物杂志, 2020(4): 202-205.
	ZHANG Haiyang, LI Haiyan, MENG Qinglin, et al. Effects of different fungicides on field control of sunflower Sclerotinia rot[J]. Crops, 2020(4): 202-205.
[10]	Tang L P, Huang Y G, Li X F, et al. Study on the control effect of 6 fungicides on Sclerotinia sclerotiorum in J]. Agricultural Science &Technology, 2023, 24(01): 51-55.
[11]	陈柳. 啶酰菌胺与咪鲜胺复配对核盘菌生长及致病的抑制机理研究[D]. 大庆: 黑龙江八一农垦大学, 2022.
	CHEN Liu. Study on the inhibitory mechanism of the combination of picrinamide and prochloraz on the growth and pathogenicity of Sclerotinia sclerotiorum[D]. Daqing: Heilongjiang Bayi Agricultural University, 2022.
[12]	牟文君, 马晓静, 胡利伟, 等. 烟草青枯病菌对4种杀菌剂的敏感性及复配药剂联合毒力评价[J]. 烟草科技, 2022, 55(3): 16-24.
	MU Wenjun, MA Xiaojing, HU Liwei, et al. Sensitivity of Ralstonia solanacearum to four fungicides and synergistic toxicity of combinatory fungicides[J]. Tobacco Science & Technology, 2022, 55(3): 16-24.
[13]	Cheng Q, Jia W, Hu C X, et al. Enhancement and improvement of selenium in soil to the resistance of rape stem against Sclerotinia sclerotiorum and the inhibition of dissolved organic matter derived from rape straw on Mycelium[J]. Environmental Pollution, 2020, 265: 114827.
[14]	梁宏杰. Strobilurins类杀菌剂吡唑醚菌酯对核盘菌生物活性的研究[D]. 武汉: 华中农业大学, 2015.
	LIANG Hongjie. Study on the bioactivity of Strobilurins fungicide pyrazolyl ester against Sclerotinia sclerotiorum[D]. Wuhan: Huazhong Agricultural University, 2015.
[15]	Ibrahim M A I, Goussous S J, Tahhan R A, et al. Evaluation of inhibition of Sclerotinia sclerotiorum (lib.) De bary by Bacillus subtilis volatile compounds in[J]. Fresenius Environmental Bulletin, 2019, 28(12A): 10139-10145.
[16]	Ding L N, Li T, Guo X J, et al. Sclerotinia stem rot resistance in rapeseed: recent progress and future prospects[J]. Journal of Agricultural and Food Chemistry, 2021, 69(10): 2965-2978. DOI PMID
[17]	Hossain M M, Sultana F, Li W Q, et al. Sclerotinia sclerotiorum (lib.) de bary: insights into the pathogenomic features of a global pathogen[J]. Cells, 2023, 12(7): 1063.
[18]	冯九焕. 中国食用向日葵育种国产化历程及研究进展[J]. 西北植物学报, 2022, 42(10): 1779-1800.
	FENG Jiuhuan. Breeding history and research advancements of confection sunflower in China[J]. Acta Botanica Boreali-Occidentalia Sinica, 2022, 42(10): 1779-1800.
[19]	李聪聪. 咯菌腈与戊唑醇复配对小麦茎基腐病及病原菌的影响[D]. 保定: 河北农业大学, 2020.
	LI Congcong. Effects of fipronil and tebuconazole on wheat stem rot and pathogen[D]. Baoding: Hebei Agricultural University, 2020.
[20]	刘翔, 姜兴印, 王雪婷, 等. 辣椒根腐病菌对咯菌腈的敏感性及其抗性突变体的生物学性状[J]. 植物保护, 2023, 49(3): 113-120, 134.
	LIU Xiang, JIANG Xingyin, WANG Xueting, et al. Sensitivity of Fusarium solani to fludioxonil and the biological characteristics of its resistant mutants[J]. Plant Protection, 2023, 49(3): 113-120, 134.
[21]	李静, 赵帅锋, 孙加焱, 等. 苯甲·嘧菌酯对几种作物炭疽病防效、安全性及品质的影响[J]. 农学学报, 2018, 8(8): 1-4. DOI
	LI Jing, ZHAO Shuaifeng, SUN Jiayan, et al. Difenoconazole·azoxystrobin: control efficiency on anthracnose, safety and effect on crop quality[J]. Journal of Agriculture, 2018, 8(8): 1-4.
[22]	符美英, 芮凯, 罗激光, 等. 苯甲·嘧菌酯防控海南稻瘟病效果初探[J]. 中国植保导刊, 2021, 41(12): 76-77, 82.
	FU Meiying, RUI Kai, LUO Jiguang, et al. Preliminary study on the control effect of benzathine azoxystrobin on rice blast in Hainan[J]. China Plant Protection, 2021, 41(12): 76-77, 82.
[23]	严百元, 李戌清, 张雅, 等. 12种杀菌剂防控莴笋菌核病效果研究[J]. 中国植保导刊, 2018, 38(5): 65-68.
	YAN Baiyuan, LI Qu\|Xu(Qing), ZHANG Ya, et al. Study on the control effect of 12 fungicides on lettuce Sclerotinia sclerotiorum[J]. China Plant Protection, 2018, 38(5): 65-68.
[24]	黄洁. 硒调控水稻响应镉与稻瘟病胁迫的作用研究[D]. 合肥: 安徽农业大学, 2022.
	HUANG Jie. Study on the role of selenium in regulating rice response to cadmium and rice blast stress[D]. Hefei: Anhui Agricultural University, 2022.
[25]	Jia W, Hu C X, Xu J Y, et al. Dissolved organic matter derived from rape straw pretreated with selenium in soil improves the inhibition of Sclerotinia sclerotiorum growth[J]. Journal of Hazardous Materials, 2019, 369: 601-610.
[26]	Li L Y, Miao S F, Qian L, et al. Sensitivity of Sclerotinia sclerotiorum to chlorofluridine and its mixtures in Henan Province[J]. Journal of Agricultural Pharmacy, 2023(4): 946-953.

Study on the inhibitory effect of fluroxonil and benzosulfuryl ester combined with selenium on Sclerotinia stem rot in sunflower

咯菌腈和苯甲·嘧菌酯与硒复配对向日葵菌核病的抑制作用

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