Effects of photosynthetic bacterial foliar fertilizer on yield and quality of organic tomato in greenhouse

doi:10.6048/j.issn.1001-4330.2023.12.017

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (12): 3018-3024.DOI: 10.6048/j.issn.1001-4330.2023.12.017

• Horticultural Special Local Products·Plant Protection • Previous Articles Next Articles

Effects of photosynthetic bacterial foliar fertilizer on yield and quality of organic tomato in greenhouse

ZENG Jun¹(), WU Lei², GAO Yan¹(), ZHANG Zhuo³, LIN Qing¹, LIU Jianwei⁴, YANG Hongmei¹, HUO Xiangdong¹, SHI Yingwu¹

1. Xinjiang Laboratory of Special Environmental Microbiology / Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Karamay Ruiheng Animal Husbandry Development Co., Ltd., Karamay Xinjiang 834008, China
3. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China
4. Xinjiang Mutianyuan Biotechnology Co. Ltd., Urumqi 830037, China

Received:2023-04-12 Online:2023-12-20 Published:2024-01-03
Correspondence author: GAO Yan(1979-),female,Jiangyin,Jiangsu Province,researcher,research field:resources and applied microbiology,(E-mail)47795411@qq.com
Supported by:
The Key Laboratory Project of Xinjiang Uygur Autonomous Region(2019D04016);The Key Technology Innovation Incubation Project of Xinjiang Academy of Agricultural Sciences(xjkcpy-2020005);Fund for Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2023005-6)

光合细菌叶面肥喷施对设施有机番茄产量和品质的影响

曾军¹(), 武磊², 高雁¹(), 张卓³, 林青¹, 刘建伟⁴, 杨红梅¹, 霍向东¹, 史应武¹

1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091
2.克拉玛依瑞恒畜牧开发有限责任公司,新疆克拉玛依 834008
3.湖南省农业科学院植物保护研究所,长沙 410125
4.新疆牧田源生物科技有限公司,乌鲁木齐 830037

通讯作者: 高雁(1979-),女,江苏江阴人,研究员,博士,硕士生导师,研究方向为资源与应用微生物学,(E-mail)47795411@qq.com
作者简介:曾军(1982-),男,新疆玛纳斯人,副研究员,研究方向为特殊环境光合细菌功能及生态学,(E-mail)leo924.student@sina.com
基金资助:
新疆维吾尔自治区重点实验室项目(2019D04016);新疆农业科学院科技创新重点培育专项(xjkcpy-2020005);农业科技创新稳定支持项目(xjnkywdzc-2023005-6)

Abstract

Abstract:

【Objective】This project aims to study the effects of photosynthetic bacterial foliar fertilizer on yield and quality of organic tomato in greenhouse in the hope of providing reference basis for leaf formula fertilization and high-efficiency production of organic tomato in greenhouse.【Methods】The tomato cultivars Zhongyan No.868 was used as the testing material. Under field conditions, three greenhouses were selected and divided into three treatment groups including PSB group, fish protein group and control group (spraying only water without fertilizer). The experiments were started at the full bearing period of tomato and fertilized every 7 days, 4 times in total. The contents of chlorophyll, net photosynthetic rate, disease index of leaves, yield and quality of tomato were determined.【Results】Compared with CK, the PSB and fish protein treatments could increase chlorophyll contents during the first 14 days after fertilization, while the PSB group was the best. The decreasing trend of the chlorophyll contents in CK and fish protein groups were highly correlated with disease index of leaves. Of them, the lowest disease index was found in the PSB group, the photosynthetic bacteria group had the lowest overall incidence index, while the fish protein group had a relatively low incidence index in the first 14 days, and the higher incidence index after 14 days, and the control group had the most serious incidence index The net photosynthetic rate in PSB and fish protein groups were increased during the first 14 days after fertilization, then decreased and slight declines occurred in PSB group, but in CK group it was the opposite. Compared with CK, the PSB group had the best tomato quality, whose soluble sugar content increased 62.5%, Vitamin C content increased 81.7% and the average yield increase per plant reached 33%. However, the fish protein group was only significantly increased in the total acid, and the difference in the improvement of other indicators was not significant.【Conclusion】For the best quality and yield effect of photosynthetic bacteria, the main mechanism may be realized by slowing down the degradation of old tomato chlorophyll and inhibiting the occurrence and development of the powdery mildew of tomato foliar leaves.

Key words: organic tomato in greenhouse; foliage fertilizer; net photosynthetic rate; quality

摘要：

【目的】研究设施番茄盛果期叶面喷施光合细菌叶面肥对其产量及品质的影响,为设施番茄高效生产及叶面施肥提供参考依据。【方法】以中研868番茄品种为研究对象,选择3个设施大棚,设置光合细菌组、鱼蛋白组、清水对照(CK,不施叶面肥)共3个处理,于番茄盛果期开始每7 d叶面喷施1次,共计喷施4次,测定各处理前和处理后不同时间段内番茄叶片叶绿素含量,净光合速率,叶片病害指数,产量和品质等指标。【结果】与对照组叶绿素含量持续下降趋势相比,喷施光合细菌和鱼蛋白后7~14 d均能提高番茄叶片叶绿素含量,之后叶绿素含量均有所下降,但光合细菌组下降幅度最低;叶绿素含量的下降与番茄叶片的病害指数呈现出显著正相关性,光合细菌组叶面白粉病全程发病指数最低,而鱼蛋白组在前14 d发病指数相对较低,而14 d之后发病指数较高,对照组发病最为严重。光合细菌组和鱼蛋白组净光合速率前14 d显著增加,之后有所降低,其中光合细菌组增加幅度最大并且下降幅度最小,对照组呈现出线性下降趋势;与对照组相比,光合细菌组番茄品质最佳,其中可溶性糖增加了62.5%,抗坏血酸提高了81.7%,平均单株产量增产率达到了33%,而鱼蛋白组仅在总酸上显著提高,其他指标提升差异不显著。【结论】设施有机番茄盛果期叶面喷施光合细菌叶面肥品质和增产效果最佳,其主要机理可能是通过减缓番茄老叶叶绿素的降解以及抑制番茄叶面白粉病的发生和发展而实现的。

关键词: 设施有机番茄, 叶面肥, 净光合速率, 品质

CLC Number:

S641.2

ZENG Jun, WU Lei, GAO Yan, ZHANG Zhuo, LIN Qing, LIU Jianwei, YANG Hongmei, HUO Xiangdong, SHI Yingwu. Effects of photosynthetic bacterial foliar fertilizer on yield and quality of organic tomato in greenhouse[J]. Xinjiang Agricultural Sciences, 2023, 60(12): 3018-3024.

曾军, 武磊, 高雁, 张卓, 林青, 刘建伟, 杨红梅, 霍向东, 史应武. 光合细菌叶面肥喷施对设施有机番茄产量和品质的影响[J]. 新疆农业科学, 2023, 60(12): 3018-3024.

Figures/Tables 4

References 28

[1]	苏品, 张德咏, 张卓, 等. 光合细菌的农用微生物功能解读[J]. 中国生物防治学报, 2021, 37(1):30-37. DOI
	SU Pin, ZHANG Deyong, ZHANG Zhuo, et al. Dissection on the Agronomical Functions of Photosynthetic Bacteria[J]. Chinese Journal of Biological Control, 2021, 37(1):30-37. DOI
[2]	Amine H M, Paloma D, Stéphane H. Microbial interactions within the plant holobiont[J]. Microbiome, 2018, 6(1):58. DOI PMID
[3]	Vorholt J A. Microbial life in the phyllosphere[J]. Nature Reviews Microbiology, 2012, 10(12):828-840. DOI PMID
[4]	Csotonyi J T, Swiderski J, Stackebrandt E, et al. A new environment for aerobic anoxygenic phototrophic bacteria: biological soil crusts[J]. Environmental Microbiology Reports, 2010, 2(5):651-656. DOI PMID
[5]	Kembel S W, O’connor T K, Arnold H K, et al. Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest[J]. Proceedings of the National Academy of Sciences of the USA, 2014, 111(38):13715-13720. DOI URL
[6]	Sasaki K, Watanabe M, Suda Y, Applications of photosynthetic bacteria for medical fields[J]. et al. Journal of Bioscience and Bioengineering, 2005, 100(5):481-488. PMID
[7]	Su P, Feng T, Zhou X, et al. Isolation of Rhp-PSP,a member of YER057c/YjgF/UK114 protein family with antiviral properties,from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b[J]. Scientific Reports, 2015, 5:16121. DOI
[8]	Su P, Tan X, Li C, et al. Photosynthetic bacterium Rhodopseudomonas palustris GJ-22 induces systemic resistance against viruses[J]. Microbial Biotechnology, 2017, 10(3):612-624. DOI PMID
[9]	张煜琦, 刘勇, 陈应凤, 等. 沼泽红假单胞菌PSB-06菌剂对番茄褪绿病的防效及其作用机制[J]. 植物保护学报, 2021, 48(6):1496-1507.
	ZHANG Yuqi, LIU Yong, CHEN Yingfeng, et al. Control efficacy and mechanism of fungicide Rhodopseudomonas palustris PSB-06 on tomato chlorosis virus[J]. Journal of Plant Protection, 2021, 48(6):1496-1507.
[10]	Luo L, Wang P, Zhai Z, et al. The effects of Rhodopseudomonas palustris PSB06 and CGA009 with different agricultural applications on rice growth and rhizosphere bacterial communities[J]. AMB Express, 2019, 9(1):173. DOI PMID
[11]	Kantachote D, Nunkaew T, Kantha T, et al. Biofertilizers from Rhodopseudomonas palustris strains to enhance rice yields and reduce methane emissions[J]. Applied Soil Ecology, 2016, 100:154-161. DOI URL
[12]	Agriculture - Crop Research. Data on Crop Research Reported by Researchers at University of Brasilia (Management of Powdery Mildew and Behaviour of Late Blight Under Different Irrigation Configurations In Organic Tomato)[J]. Agriculture Week, 2019.
[13]	Choudhary D K, Prakash A, Johri B N. Induced systemic resistance (ISR) in plants: mechanism of action[J]. Indian Journal of Microbiology, 2007, 47(4):289-297. DOI PMID
[14]	Lee K H, Koh R H, Song H G. Enhancement of growth and yield of tomato by Rhodopseudomonas sp. under greenhouse conditions[J]. The Journal of Microbiology, 2008, 46(6):641-646. DOI URL
[15]	Sasaki K, Watanabe M, Suda Y, et al. Applications of photosynthetic bacteria for medical fields[J]. Journal of Bioscience and Bioengineering, 2005, 100(5):481-488. PMID
[16]	李兴发, 黄娟, 杨雅婷, 等. 光合细菌菌剂对黄冠梨土壤微生物区系及果实品质的影响[J]. 西南师范大学学报(自然科学版), 2015, 40 (6):55-61.
	LI Xingfa, HUANG Juan, YANG Yating, et al. On effects of inoculant of photosynthetic bacterica on soil microbial community and fruit quality of ‘Huangguan’ pear[J]. Journal of Southwest China Normal University(Natural Science Ed.), 2015, 40(6):55-61.
[17]	Kwak M J, Jeong H, Madhaiyan M, et al. Genome information of Methylobacterium oryzae, a plant-probiotic methylotroph in the phyllosphere[J]. PLoS ONE, 2014, 9(9):e106704. DOI URL
[18]	Idi A, Md Nor M H, Abdul Wahab M F, et al. Photosynthetic bacteria: an eco-friendly and cheap tool for bioremediation[J]. Reviews in Environmental Science and Biotechnology, 2014, 14(2):271-285. DOI URL
[19]	刘珍珠. 紫色非硫光合细菌分离鉴定及其脱氮性能的初探[D]. 乌鲁木齐: 新疆大学, 2019.
	LIU Zhenzhu. Isolation and Identification of Purple Non-sulfur Photosynthetic Bacteria and Preliminary Study on Its Nitrogen Removal Performance[D]. Urumqi: Xinjiang University, 2019.
[20]	Jun Z, Kai L, Cui-Jing Z, et al. Primary Succession of Nitrogen Cycling Microbial Communities along the Deglaciated Forelands of Tianshan Mountain, China[J]. Frontiers in Microbiology, 2016, (7):1353.
[21]	杨芳, 田俊岭, 杨盼盼, 等. 高效光合细菌菌剂对番茄品质、土壤肥力及微生物特性的影响[J]. 华南农业大学学报, 2014, 35 (1):49-54.
	YANG Fang, TIAN Junling, YANG Panpan, et al. Effects of inoculants of photosynthetic bacteria on tomato quality, soil fertility and soil microbial characteristics[J]. Journal of South China Agricultural University, 2014, 35 (1):49-54.
[22]	赵芮晗, 张欢, 卢海凤, 等. 追施光合细菌与猪场粪污对生菜生长效果的研究[J]. 河南农业大学学报, 2020, 54(5):786-792.
	ZHAO Ruihan, ZHANG Huan, LU Haifeng, et al. Study on the effect of applying photosynthetic bacteria and pig farm manure on the growth of lettuce[J]. Journal of Henan Agricultural University, 2020, 54(5):786-792.
[23]	赵志强, 文孝荣, 高雁, 等. 光合细菌与尿素联合施用对土壤理化性质和酶活性及水稻产量和加工品质的影响[J]. 中国稻米, 2021, 27 (5):105-110. DOI
	ZHAO Zhiqiang, WEN Xiaorong, GAO Yan, et al. Effects of combined application of photosynthetic bacteria and urea on soil physical and chemical properties, enzyme activities, and rice yield and procession quality[J]. China Rice, 2021, 27(5):105-110. DOI
[24]	张钧恒. 设施番茄全有机营养肥水耦合效应研究[D]. 杨凌: 西北农林科技大学, 2018.
	ZHANG Junheng. Study on the effect of all-organic nutrient solution and water coupling on tomato[D]. Yangling: Northwest A and F University, 2018.
[25]	王归鹏. 设施番茄全有机栽培营养供给研究[D]. 杨凌: 西北农林科技大学, 2021.
	WANG Guipeng. Research on nutrient supply of fully organic cultivation of protected tomato[D]. Yangling: Northwest A and F University, 2021.
[26]	董美云. 设施番茄有机生产技术[J]. 中国瓜菜, 2019, 32(7):73-75.
	DONG Meiyun. Organic tomato producing technology in greenhouse[J]. China Cucurbits and Vegetables, 2019, 32(7):73-75.
[27]	高炜, 杨三维, 李世平, 等. 光合细菌对小麦生长和光合功能的影响[J]. 微生物学通报, 2014, 41 (6):1152-1159.
	GAO Wei, YANG Sanwei, LI Shiping, et al. Effects of photosynthetic bacteria on growth and photosynthetic function in wheat[J]. Microbiology China, 2014, 41 (6):1152-1159.
[28]	张欢, 卢海凤, 孟涛, 等. 光合细菌在种植业上的应用研究进展[J]. 江苏农业科学, 2020, 48 (8):18-28.
	ZHANG Huan, LU Haifeng, MENG Tao, et al. Research progress on application of photosynthetic bacteria in planting industry[J]. Jiangsu Agricultural Sciences, 2020, 48(8):18-28.

处理组 Treatment group	叶绿素(a+b)Chlorophyll (a+b) (mg/g·FW)
处理组 Treatment group	喷施前Before spraying	7 d	14 d	21 d	28 d
光合细菌组 PSB group	1.41±0.02^b	1.87±0.012^a*	2.49±0.002^a**	1.84±0.011^A*	1.74±0.016^A*
鱼蛋白组 Fish protein group	1.51±0.21^a	1.78±0.062^b*	1.87±0.13^b**	1.20±0.42^B*	1.1±0.10^B*
对照组 CK	1.49±0.43^a	1.51±0.12^b*	1.21±0.11^c*	0.77±0.12^C**	0.87±0.33^C**

处理组 Treatment group	叶绿素(a+b)Chlorophyll (a+b) (mg/g·FW)
处理组 Treatment group	喷施前Before spraying	7 d	14 d	21 d	28 d
光合细菌组 PSB group	1.41±0.02^b	1.87±0.012^a*	2.49±0.002^a**	1.84±0.011^A*	1.74±0.016^A*
鱼蛋白组 Fish protein group	1.51±0.21^a	1.78±0.062^b*	1.87±0.13^b**	1.20±0.42^B*	1.1±0.10^B*
对照组 CK	1.49±0.43^a	1.51±0.12^b*	1.21±0.11^c*	0.77±0.12^C**	0.87±0.33^C**

处理组 Treatment group	喷施前发病指数 Disease index before usage	喷施后发病指数 Disease index after usage
处理组 Treatment group	喷施前发病指数 Disease index before usage	7 d	14 d	21 d	28 d
光合细菌组 PSB group	0.51±0.032^a	0.37±0.012^B**	0.29±0.002^C**	0.44±0.011^C*	0.47±0.016^C*
鱼蛋白组 Fish protein group	0.48±0.021^a	0.58±0.062^A*	0.87±0.13^B**	1.27±0.42^B**	1.97±0.10^A**
对照组 CK	0.49±0.043^a	0.67±0.012^A*	1.61±0.11^A**	1.77±0.12^A**	1.37±0.33^B**

处理组 Treatment group	喷施前发病指数 Disease index before usage	喷施后发病指数 Disease index after usage
处理组 Treatment group	喷施前发病指数 Disease index before usage	7 d	14 d	21 d	28 d
光合细菌组 PSB group	0.51±0.032^a	0.37±0.012^B**	0.29±0.002^C**	0.44±0.011^C*	0.47±0.016^C*
鱼蛋白组 Fish protein group	0.48±0.021^a	0.58±0.062^A*	0.87±0.13^B**	1.27±0.42^B**	1.97±0.10^A**
对照组 CK	0.49±0.043^a	0.67±0.012^A*	1.61±0.11^A**	1.77±0.12^A**	1.37±0.33^B**

处理 Treatment	叶面净光合速率 net photosynthetic rate of tomato leaves [μmolCO₂/(m²﹒s)]
处理 Treatment	喷施前 Before spraying	喷施7 d Spray 7 d	喷施14 d Spray 14 d	喷施21 d Spray 21 d	喷施28 d Spray 28 d
光合细菌组 PSB group	12.58±1.03^a	16.56±0.25^A	27.33±0.31^A**	14.03±0.33^A	12.73±1.79^A
鱼蛋白组 Fish protein group	14.25±0.76^a	13.43±1.74^a	12.51±0.67^B	8.12±0.22^B*	8.77±1.01^B*
对照组CK	13.10±1.01^a	10.93±3.48^a	8.67±0.73^C*	6.00±0.98^B*	5.57±0.21^C*

Effects of photosynthetic bacterial foliar fertilizer on yield and quality of organic tomato in greenhouse

光合细菌叶面肥喷施对设施有机番茄产量和品质的影响

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 4

References 28

Related Articles 15

Recommended Articles 0

Metrics

处理 Treatment	可溶性糖 Soluble saccharide (%)	总酸 Total acidity (%)	可溶性固形物 Soluble solids (%)	抗坏血酸 Vitamin C (mg/100g)	产量 Yield (kg/株)
光合细菌组PSB group	3.96±0.18^A	7.10±1.53^B	5.79±0.22^a	5.09±0.22^A	3.29±0.82^a
鱼蛋白组Fish protein group	2.63±0.20^B	9.23±1.15^A	4.12±0.58^a	1.69±0.58^C	3.01±0.28^b
对照组CK	2.40±0.06^B	5.60±1.45^C	4.83±0.56^a	2.80±0.56^B	2.46±0.56^b

[1]	JIN Juan, LI lili, YANG Lei, FAN Dingyu, HAO Qing. Analysis on the Development status of Xinjiang Jujube Industry [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 106-110.
[2]	WU Yating, CHEN He, ZHENG Nan, MA Xianlan, ZHOU Lina, ZHAO Yankun. Current situation and development trend prospect of Xinjiang characteristic dairy industry [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 158-162.
[3]	HOU Xianzheng, XIAO Tong, CHEN Yulan, WEI Jiyu. The spatial effects and mechanism of digital technology innovation on agricultural economic resilience [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 196-205.
[4]	YUE Rongqiang, ZHANG Qiong, WANG Fang, DENG Wenwen, CHEN Yu, Maiwulanjiang Mamut, Nurmanquli Batur. Improve the academic quality and influence of agricultural academic journals [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 256-260.
[5]	WANG Junduo, CUI Yujiang, LIANG Yajun, GONG Zhaolong, ZHENG Junyun, LI Xueyuan. Xinjiang cotton production advantageous regional layout scheme [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 60-69.
[6]	FANG Hui, DING Yindeng, FAN Guiqiang, GAO Yonghong, HUANG Tianrong. Research report on the development status of wheat industry in southern Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 75-80.
[7]	YANG Minghua, LIAO Biyong, LIU Qiang, PENG Yuncheng, Dawulai Jiekeshan, FENG Guorui, TANG Shimin. Study on variation of grain nutritional quality of glutinous maize [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2087-2093.
[8]	ZHANG Zehua, YE Hanchun, WANG Zhenhua, LI Wenhao, LI Haiqiang, LIU Jian. Effects of equal nitrogen applied with urease inhibitor on cotton growth, yield, and quality under mulched drip irrigation [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2103-2111.
[9]	CHEN Ruijie, LUO Linyi, RUAN Xiangyang, YE Jun. Effects of humic acid on soil nutrients, cotton yield and quality in cotton fields under drip irrigation [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2112-2121.
[10]	LIU Jing, DU Mingchuan, ZHANG Wenting, BAO Haijuan, JING Meiling, DU Wenhua. Screening of triticale germplasm in different areas of Qinghai [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2183-2190.
[11]	TIAN Haiyan, ZHANG Zhanqin, XIE Jianhui, WANG Jianjiang, YANG Xiangkun. Study on the relationship between Lycopene and main quality characters of processing tomato [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2197-2202.
[12]	ZHANG Tingjun, LI Zihui, CUI Yujiang, SUN Xiaogui, CHEN Fang. Effects of microbial agents on cotton growth and soil physico-chemical properties [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2269-2276.
[13]	CHEN Fang, LI Zihui, SUNXiaogui , ZHANG Tingjun. Different dosage of microbial agents on the yield and quality of processed tomatoes [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2285-2289.
[14]	QIAO Yajie, FU Huixin, QIAO Xue, MENG Xintao, ZHANG Ting, PAN Yan. Study on the variation of fresh beef quality under different storage temperature conditions [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2323-2329.
[15]	ZHANG Chengjie, HU Haoran, DUAN Songjiang, WU Yifan, ZHANG Jusong. Effects of nitrogen-dense interaction on growth, development, yield and quality of Gossypium barbadense L. [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1821-1830.