Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress

doi:10.6048/j.issn.1001-4330.2023.02.012

Abstract

Abstract:

【Objective】 To study the effect of exogenous GSNO (exogenous NO donor) on alleviating NaCl stress of tomato. 【Methods】 In this study, tomato cultivar Zhongshu 4 was used as experimental material. Tomato Seedlings under NaCl stress (100 mmol/L) were treated with NG:0.5 mmol/L GSNO (S-nitrosoglutathione, NO donor), NP: 1 mmol/L cPTIO (NO scavenger), NGP: 0.5 mmol/L GSNO compound 1 mmol/L cPTIO; The effects of exogenous GSNO on the growth and salt tolerance of tomato seedlings under NaCl stress were studied. 【Results】 After applying GSNO, the growth of tomato seedlings was significantly improved under NaCl stress, the chlorophyll content increased, the chlorophyll fluorescence parameters returned to normal, and the Calvin cycle enzyme activity returned to normal level. 【Conclusion】 This study shows that exogenous GSNO can alleviate the adverse growth and photosynthetic effect of tomato seedlings under NaCl stress.

Key words: tomato; GSNO; NaCl stress; photosynthesis

摘要：

【目的】 研究NaCl胁迫下外源GSNO(NO供体)对番茄幼苗生长的调节作用。【方法】 以中蔬4号番茄为材料,通过对100 mmol/L NaCl胁迫下番茄幼苗分别喷施0.1 mmol/L GSNO(NO供体)(NG处理)、0.05 mmol/L cPTIO(NO清除剂)(NP处理)、0.1 mmol/L GSNO + 0.05 mmol/L cPTIO (NGP处理),研究外源GSNO对NaCl胁迫下番茄幼苗生长及其盐适应性的调控作用。【结果】 NaCl胁迫下施用GSNO的番茄幼苗生长情况明显好转,叶绿素含量和最大光化学效率(Fv/Fm)提高,Rubisco初始活性和总活性提高,卡尔文循环相关酶Rubisco活化酶(RCA)、磷酸甘油酸激酶(PGK)、磷酸甘油醛脱氢酶(GAPDH)、景天庚酮糖-1,7-二磷酸酶(SBPase)和果糖-1,6-二磷酸酶(FBPase)的活性提高。【结论】 外源GSNO对NaCl胁迫下番茄幼苗生长不良和光合作用降低有缓解作用。

关键词: 番茄, GSNO, NaCl胁迫, 光合作用

CLC Number:

S641.2

ZHU Pusheng, LIU Huiying, CAO Ze, LIU Kaige, LI Xuezhen. Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 351-358.

朱普生, 刘慧英, 曹泽, 刘凯歌, 李雪珍. 外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响[J]. 新疆农业科学, 2023, 60(2): 351-358.

Figures/Tables 5

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处理 Treatment	株高 Plant height (cm)	茎粗 Stem diameter (mm)	地上鲜重 Fresh weight over water (g)	地下鲜重 Fresh weight under water (g)	地上干重 Dry weight over water (g)	地下干重 Dry weight under water (g)	根冠比 Root/shoot ratio	壮苗指数 Strong seedling index
CK	29.44±0.69^a	7.77±0.18^a	30.66±2.17^a	4.56±0.22^ab	2.16±0.24^a	0.18±0.01^a	0.08±0.01^b	0.81±0.06^a
NaCl	20.29±0.2^cd	6.32±0.31^c	12.88±0.67^c	3.94±0.26^bc	0.97±0.04^c	0.12±0.00^b	0.13±0.00^a	0.48±0.02^c
NG	22.62±0.3^b	6.74±0.08^bc	19.52±0.86^b	5.32±0.4^a	1.5±0.06^b	0.2±0.02^a	0.14±0.01^a	0.74±0.04^ab
NP	19.53±0.98^d	6.76±0.19^bc	15.64±1.1^bc	3.3±0.18^c	1.22±0.09^bc	0.14±0.01^b	0.11±0.00^a	0.62±0.03^b
NGP	22.19±0.75^bc	7.28±0.04^ab	18.77±0.9^b	4.61±0.41^ab	1.45±0.08^b	0.18±0.02^a	0.13±0.01^a	0.74±0.04^ab

处理 Treatment	株高 Plant height (cm)	茎粗 Stem diameter (mm)	地上鲜重 Fresh weight over water (g)	地下鲜重 Fresh weight under water (g)	地上干重 Dry weight over water (g)	地下干重 Dry weight under water (g)	根冠比 Root/shoot ratio	壮苗指数 Strong seedling index
CK	29.44±0.69^a	7.77±0.18^a	30.66±2.17^a	4.56±0.22^ab	2.16±0.24^a	0.18±0.01^a	0.08±0.01^b	0.81±0.06^a
NaCl	20.29±0.2^cd	6.32±0.31^c	12.88±0.67^c	3.94±0.26^bc	0.97±0.04^c	0.12±0.00^b	0.13±0.00^a	0.48±0.02^c
NG	22.62±0.3^b	6.74±0.08^bc	19.52±0.86^b	5.32±0.4^a	1.5±0.06^b	0.2±0.02^a	0.14±0.01^a	0.74±0.04^ab
NP	19.53±0.98^d	6.76±0.19^bc	15.64±1.1^bc	3.3±0.18^c	1.22±0.09^bc	0.14±0.01^b	0.11±0.00^a	0.62±0.03^b
NGP	22.19±0.75^bc	7.28±0.04^ab	18.77±0.9^b	4.61±0.41^ab	1.45±0.08^b	0.18±0.02^a	0.13±0.01^a	0.74±0.04^ab

指标 Index	处理 Treatment
指标 Index	CK	N	NG	NP	NGP
初始活性Rubisco(U/g)	9.85±0.27^a	6.61±0.34^c	8.67±0.27^b	5.40±0.50^d	8.20±0.42^b
总活性Rubisco(U/g)	30.82±0.72^a	12.59±0.28^d	24.34±1.27^b	15.40±0.66^c	17.10±0.82^c
活化酶Rubisco(RCA)活性(mU/g)	1 827.33±87.05^a	1 221.15±83.85^d	1 672.63±22.10^b	1 114.68±36.29^e	1 356.19±7.71^c
磷酸甘油酸激酶(PGK)活性(U/g)	1.79±0.02^a	1.40±0.04^c	1.55±0.06^b	1.15±0.05^d	1.44±0.02^c
磷酸甘油醛脱氢酶(GAPDH)活性(U/g)	0.47±0.01^a	0.24±0.01^d	0.42±0.01^b	0.21±0.01^e	0.40±0.01^c
景天庚酮糖-1,7-二磷酸酶(SBPase) 活性(U/g)	1.31±0.10^a	0.43±0.01^d	0.67±0.05^c	0.48±0.02^d	0.78±0.01^b
果糖-1,6-二磷酸酶(FBPase) 活性(mU/g)	937.38±10.82^a	837.33±3.11^b	936.85±7.80^a	794.68±10.80^c	857.13±29.93^b
果糖-1,6-二磷酸醛缩酶(FBA) 活性(mU/g)	1.38±0.03^a	1.27±0.04^b	1.22±0.05^b	1.01±0.02^c	1.28±0.03^b

指标 Index	处理 Treatment
指标 Index	CK	N	NG	NP	NGP
初始活性Rubisco(U/g)	9.85±0.27^a	6.61±0.34^c	8.67±0.27^b	5.40±0.50^d	8.20±0.42^b
总活性Rubisco(U/g)	30.82±0.72^a	12.59±0.28^d	24.34±1.27^b	15.40±0.66^c	17.10±0.82^c
活化酶Rubisco(RCA)活性(mU/g)	1 827.33±87.05^a	1 221.15±83.85^d	1 672.63±22.10^b	1 114.68±36.29^e	1 356.19±7.71^c
磷酸甘油酸激酶(PGK)活性(U/g)	1.79±0.02^a	1.40±0.04^c	1.55±0.06^b	1.15±0.05^d	1.44±0.02^c
磷酸甘油醛脱氢酶(GAPDH)活性(U/g)	0.47±0.01^a	0.24±0.01^d	0.42±0.01^b	0.21±0.01^e	0.40±0.01^c
景天庚酮糖-1,7-二磷酸酶(SBPase) 活性(U/g)	1.31±0.10^a	0.43±0.01^d	0.67±0.05^c	0.48±0.02^d	0.78±0.01^b
果糖-1,6-二磷酸酶(FBPase) 活性(mU/g)	937.38±10.82^a	837.33±3.11^b	936.85±7.80^a	794.68±10.80^c	857.13±29.93^b
果糖-1,6-二磷酸醛缩酶(FBA) 活性(mU/g)	1.38±0.03^a	1.27±0.04^b	1.22±0.05^b	1.01±0.02^c	1.28±0.03^b