Morphological and ISSR Analysis of Purple Sweet Potato Resources

doi:10.6048/j.issn.1001-4330.2022.07.008

Abstract

Abstract:

【Objective】 To analyze the genetic diversity of purple sweet potato varieties collected from China, clarify their genetic differences in the hope of providing a theoretical basis for the selection of purple sweet potato genetic breeding parents. 【Methods】 Morphological characters and ISSR fluorescent labeling capillary electrophoresis were carried out to analyze the genetic diversity of varieties of 10 purple sweet potatoes imported from other provinces. 【Results】 The results showed that, based on the cluster map of 20 morphological traits, the tested materials could be divided into two major groups at the Euclidean distance of 9.97. The first major category included Ningzi-1, Shandongzi, Yuzi-2, Xuzi-8, Yuenanzi; the second major category consisted of E-12, Ningzi-4, Yuzi-3, Yuzi-11, Nanzi-018. 6 pairs of primers were used in ISSR molecular marker analysis, the results showed that, the observed alleles (Na) of the purple sweet potato germplasm resources were 2, the average effective allele number (Ne) was 1.9190, and the expected heterozygosity (He) was 0.2375 and Shannon's index (I) was 0.4333. Cluster analysis performed with NTSYSpc 2.1 software demonstrated that when the genetic distance was 0.96, the 10 tested purple sweet potato materials could be divided into two categories. 【Conclusion】 The clustering results of SSR markers and morphological markers of several tested materials were consistent in genetic background and group classification, but the cluster analysis results of the two identification and classification methods also had certain differences. The purple sweet potato resources presented very rich genetic diversity. The combination of morphology and ISSR markers could effectively improve the identification of specific varieties and determine the genetic relationship of germplasm resources objectively and accurately.

Key words: purple sweet potato; morphology; cluster analysis; fluorescent ISSR markers; genetic diversity

摘要：

【目的】分析紫色甘薯品种的遗传多样性,其遗传差异,为紫色甘薯遗传育种亲本选择提供理论依据。【方法】以引进的10份紫色甘薯为材料,利用形态学性状和ISSR 荧光标记毛细管电泳技术分析品种遗传多样性。【结果】基于20项形态学性状的聚类图,在欧式距离为9.97处,可分为两大类群,第一大类为宁紫1号、山东紫薯、渝紫2号、徐紫8号、越南紫薯 ;第二大类为鄂12、宁紫4号、渝紫3号、渝紫11、南紫018 。紫色甘薯种质资源的观测等位基因为(Na)为2个,平均有效等位基因数(Ne)为1.919 0,期望杂合度(He)为0.237 5,香农指数(I)为0.433 3,在遗传距离为0.96时,10份供试紫色甘薯材料可分为两大类。【结论】部分供试材料的ISSR标记聚类结果与形态学标记在遗传背景和类群划分上具有一致性,但是两种鉴定分类方法的聚类分析结果也存在一定差异。紫色甘薯种质材料遗传多样性丰富,将形态学及ISSR标记结合能有效提高品种间特异性的鉴定,更能客观、准确的确定种质资源的亲缘关系。

关键词: 紫色甘薯, 形态学, 聚类分析, ISSR荧光标记, 遗传多样性

CLC Number:

S531

WU Qiaoyu, HE Tianjiu. Morphological and ISSR Analysis of Purple Sweet Potato Resources[J]. Xinjiang Agricultural Sciences, 2022, 59(7): 1625-1631.

吴巧玉, 何天久. 紫色甘薯资源的形态学和ISSR荧光标记分析[J]. 新疆农业科学, 2022, 59(7): 1625-1631.

Figures/Tables 6

References 17

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编号 No.	材料名称 Material name	来源 Source	编号 No.	材料名称 Material name	来源 Source
1	宁紫薯1号	西南大学	6	渝紫薯3号	西南大学
2	鄂12	西南大学	7	渝紫薯2号	西南大学
3	渝紫11	西南大学	8	越南紫薯	山东
4	南紫018	西南大学	9	徐紫薯8号	江苏
5	宁紫薯4号	西南大学	10	山东紫薯	山东

编号 No.	材料名称 Material name	来源 Source	编号 No.	材料名称 Material name	来源 Source
1	宁紫薯1号	西南大学	6	渝紫薯3号	西南大学
2	鄂12	西南大学	7	渝紫薯2号	西南大学
3	渝紫11	西南大学	8	越南紫薯	山东
4	南紫018	西南大学	9	徐紫薯8号	江苏
5	宁紫薯4号	西南大学	10	山东紫薯	山东

形态标记 Morphological trait	形态种类 Morphological category	编码数 Code number	编码类别 Coding category
顶芽色Terminal Bud Color	1黄绿;2.绿;3浅紫;4.紫;5.深紫;6.褐	6	多态性状
顶叶形状Parietal shape	1.圆形;2.肾形;3.心形;4.尖心;5.三角;6.缺刻	6	多态性状
顶叶色Parietal color	1.黄绿;2.绿;3.褐绿;4.紫绿;5.褐;6.红;7.浅紫;8.紫	8	多态性状
顶叶叶缘色Apical leaf margin color	1.黄绿;2.绿;3.红;4.紫;5.褐	5	多态性状
叶片形状Blade shape	1.圆形;2.肾形;3.心形;4.尖心;5.三角形;6.缺刻	6	多态性状
叶缘类型Leaf margin type	1.全缘;2.带齿;3.浅刻缺;4.中等刻缺;5.深刻缺;6.极度刻缺	6	多态性状
叶缺刻类型Foliation type	1.非缺刻;2.单缺刻;3.复缺刻	3	多态性状
叶缺刻数Foliation number	1.无;2.1对;3.2对;4.3对;5.多对	5	多态性状
叶尖形状Tip shape	1.无;2.锐;3.钝	3	多态性状
叶色Leaf Colour	1.黄绿;2.绿;3.褐绿;4.紫绿;5.褐;6.红	6	多态性状
叶缘色Leaf margin color	1.黄绿;2.绿;3.红;4.紫;5.褐	5	多态性状
叶脉色Vein color	1.黄;2.绿;3.主脉基部紫斑;4.数条叶脉紫斑;5.主脉部分紫;6.主脉全紫;7.全部叶脉部分紫;8.全部叶脉紫	8	多态性状
叶柄色Petiole color	1.黄绿;2.绿3.绿基部带紫;4.绿上部带紫;5.绿带紫斑;6.绿带紫条;7.紫上部带绿;8.部分叶柄紫色/绿色绿色;9.紫	9	多态性状
茎主色Dominant color of stem	1.绿;2.绿带少量紫斑;3.绿带大量紫斑;4.绿带少量褐斑;5.绿带大量褐斑;6.顶部紫;7.节点紫;8.其他	8	多态性状
节间长Internode length	1.极短 ;2.短 ;3.中等 ;4.长 ;5.极长	5	多态性状
主蔓长Main tendril	1.短 ;2.中 ;3.长 ;4.特长	4	多态性状
薯形Potato shape	1.球形;2.短纺锤;3.纺锤;4.上膨纺;5.下膨纺;6.筒形;7.长筒形;8.长纺锤;9.不规则	9	多态性状
薯皮光滑度Smoothness of potato skin	1.光滑;2.较光滑;3.粗糙	3	多态性状
薯皮主色Main color of potato skin	1.白;2.浅黄;3.黄;4.橘红;5.棕红;6.粉红;7.紫红;8.紫;9.深紫	9	多态性状
薯肉主色Main color of sweet potato meat	1.白;2.浅黄;3.黄;4.橘黄;5.橘红;6.粉红;7.红;8.紫;9.深紫	9	多态性状

形态标记 Morphological trait	形态种类 Morphological category	编码数 Code number	编码类别 Coding category
顶芽色Terminal Bud Color	1黄绿;2.绿;3浅紫;4.紫;5.深紫;6.褐	6	多态性状
顶叶形状Parietal shape	1.圆形;2.肾形;3.心形;4.尖心;5.三角;6.缺刻	6	多态性状
顶叶色Parietal color	1.黄绿;2.绿;3.褐绿;4.紫绿;5.褐;6.红;7.浅紫;8.紫	8	多态性状
顶叶叶缘色Apical leaf margin color	1.黄绿;2.绿;3.红;4.紫;5.褐	5	多态性状
叶片形状Blade shape	1.圆形;2.肾形;3.心形;4.尖心;5.三角形;6.缺刻	6	多态性状
叶缘类型Leaf margin type	1.全缘;2.带齿;3.浅刻缺;4.中等刻缺;5.深刻缺;6.极度刻缺	6	多态性状
叶缺刻类型Foliation type	1.非缺刻;2.单缺刻;3.复缺刻	3	多态性状
叶缺刻数Foliation number	1.无;2.1对;3.2对;4.3对;5.多对	5	多态性状
叶尖形状Tip shape	1.无;2.锐;3.钝	3	多态性状
叶色Leaf Colour	1.黄绿;2.绿;3.褐绿;4.紫绿;5.褐;6.红	6	多态性状
叶缘色Leaf margin color	1.黄绿;2.绿;3.红;4.紫;5.褐	5	多态性状
叶脉色Vein color	1.黄;2.绿;3.主脉基部紫斑;4.数条叶脉紫斑;5.主脉部分紫;6.主脉全紫;7.全部叶脉部分紫;8.全部叶脉紫	8	多态性状
叶柄色Petiole color	1.黄绿;2.绿3.绿基部带紫;4.绿上部带紫;5.绿带紫斑;6.绿带紫条;7.紫上部带绿;8.部分叶柄紫色/绿色绿色;9.紫	9	多态性状
茎主色Dominant color of stem	1.绿;2.绿带少量紫斑;3.绿带大量紫斑;4.绿带少量褐斑;5.绿带大量褐斑;6.顶部紫;7.节点紫;8.其他	8	多态性状
节间长Internode length	1.极短 ;2.短 ;3.中等 ;4.长 ;5.极长	5	多态性状
主蔓长Main tendril	1.短 ;2.中 ;3.长 ;4.特长	4	多态性状
薯形Potato shape	1.球形;2.短纺锤;3.纺锤;4.上膨纺;5.下膨纺;6.筒形;7.长筒形;8.长纺锤;9.不规则	9	多态性状
薯皮光滑度Smoothness of potato skin	1.光滑;2.较光滑;3.粗糙	3	多态性状
薯皮主色Main color of potato skin	1.白;2.浅黄;3.黄;4.橘红;5.棕红;6.粉红;7.紫红;8.紫;9.深紫	9	多态性状
薯肉主色Main color of sweet potato meat	1.白;2.浅黄;3.黄;4.橘黄;5.橘红;6.粉红;7.红;8.紫;9.深紫	9	多态性状

形态标记 Morphological trait	材料编号 No. of Varieties
形态标记 Morphological trait	1	2	3	4	5	6	7	8	9	10
顶芽色Terminal Bud Color	1	3	3	4	4	3	1	1	3	1
顶叶形状Parietal shape	6	6	4	5	6	6	6	4	6	4
顶叶色Parietal color	1	7	7	8	8	7	1	2	4	2
顶叶叶缘色Apical leaf margin color	4	4	4	4	4	4	4	2	4	4
叶片形状Blade shape	6	6	4	5	6	6	6	4	6	5
叶缘类型Leaf margin type	3	4	4	1	3	2	5	1	3	3
叶缺刻类型Foliation type	2	2	2	1	2	2	3	1	2	2
叶缺刻数Foliation number	2	2	2	1	2	2	3	1	2	2
叶尖形状Tip shape	2	2	2	2	2	2	2	2	2	2
叶色Leaf Colour	2	2	2	2	2	2	2	2	2	2
叶缘色Leaf margin color	2	2	2	2	2	4	2	2	2	2
叶脉色Vein color	2	8	8	8	5	7	2	2	2	2
叶柄色Petiole color	2	4	4	4	3	4	2	3	2	2
茎主色Dominant color of stem	2	2	3	2	2	2	1	3	3	3
节间长Internode length	2	1	1	1	1	1	1	1	3	2
主蔓长Main tendril	1	1	1	1	1	1	1	1	3	2
薯形Potato shape	8	3	7	7	2	1	7	8	8	8
薯皮光滑度Smoothness of potato skin	1	1	3	3	1	3	3	1	3	3
薯皮主色Main color of potato skin	8	8	9	8	9	9	8	9	9	9
薯肉主色Main color of sweet potato meat	8	8	9	8	8	8	8	9	8	8