Morphological Observation and Physio-Biochemical Characteristics of Hot Pepper Genic Male - Sterile Line pby-1

doi:10.6048/j.issn.1001-4330.2023.03.013

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (3): 624-632.DOI: 10.6048/j.issn.1001-4330.2023.03.013

• Horticultural Special Local Products·Physiology and Biochemistry • Previous Articles Next Articles

Morphological Observation and Physio-Biochemical Characteristics of Hot Pepper Genic Male - Sterile Line pby-1

PEI Hongxia¹(), LI Shengmei², WU Xuxia³, GENG Shiwei², LAI Lili⁴, GAO Jingxia¹(), DONG Xinjiu²()

1. Institute of Horticulture, Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
2. Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
3. Hohhot vocational college, Huhehaote, 010070, China
4. Xinjiang Tianjiao Hong'an Agricultural Technology Co., Shihezi Xinjiang 832011, China

Received:2022-07-30 Online:2023-03-20 Published:2023-04-18
Correspondence author: GAO Jingxia (1984-), female, from ningxia, research assistant, vegetable genetics and breeding research, (E-mail)774350762@qq.com;
DONG Xinjiu (1980-), male, from Shandong, research asociate, crop cultivation and physiological research, (E-mail)80416913@qq.com
Supported by:
Ningxia Natural Science Union Foundation(2021AAC03264);Agricultural Science and Technology Independent Innovation Fund of Ningxia Hui Autonomous Region(NGSB-2021-8-01);Outstanding Young Scientific and Technological Talents Training Program of Xinjiang Uygur Autonomous Region(2018Q16)

辣椒核雄性不育系pby-1形态学及生理生化特性分析

裴红霞¹(), 李生梅², 武旭霞³, 耿世伟², 赖黎丽⁴, 高晶霞¹(), 董心久²()

1.宁夏农林科学院园艺研究所,银川 750002
2.新疆农业科学院经济作物研究所,乌鲁木齐 830091
3.呼和浩特职业学院,呼和浩特 010070
4.新疆天椒红安农业科技有限责任公司,新疆石河子 832011

通讯作者: 高晶霞(1984-), 女, 宁夏人,助理研究员, 研究方向为作物遗传育种, (E-mail)774350762@qq.com;
董心久(1980-), 男, 山东人, 副研究员, 研究方向为作物栽培与生理, (E-mail)80416913@qq.com
作者简介:裴红霞(1980-), 女, 山西人,助理研究员, 研究方向为作物遗传育种, (E-mail)810444147@qq.com
基金资助:
宁夏自然科学联合基金(2021AAC03264);宁夏回族自治区农业科技自主创新资金(NGSB-2021-8-01);新疆维吾尔自治区优秀青年科技人才培养项目(2018Q16)

Abstract

Abstract:

【Objective】 This project aims to explore the morphological, physio-biochemical characteristics of male sterile lines of pepper (Capsicum annuum L.) in the hope of providing a theoretical reference for revealing the genetic mechanism of male sterility of hot pepper.【Methods】 The pepper nuclear male sterility line pby-1 and the maintainer line PBY-1 were used as test materials.The development of flower buds at 7 stages and the flower organs in the full blooming period were observed, and the viability of the pollen in the full blooming period was measured.The contents of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA) in flower buds at the three stages of meiosis number, tetrad and mononuclear were analyzed.The contents of gibberelli cacid (GA₃), acetic acid (IAA), zeatin riboside (ZR), jasmonic acid (JA) and abscisic acid (ABA) and the balance relationship between different endogenous hormones were compared.【Results】 Compared with the wild-type PBY-1, the sterile line only differed from its floral organ structure, the anthers were shriveled, and the pollen had no viability.In the three stages of flower bud development of hot pepper, there were differences in protective enzyme activity and endogenous hormone content between the sterile line and the maintainer line.Among them, the SOD activity of the sterile line was always significantly lower than that of the maintainer line, while the CAT activity was always significantly higher than that of the maintainer line, POD activity was significantly lower than that of the maintainer line during the meiotic and tetrad period, and the MDA content was significantly higher than that of the maintainer line only in the mononuclear period.In the three periods, there were differences in protective enzyme activity and endogenous hormone content between the sterile line and the maintainer line in the development of hot pepper.Among them, the SOD activity of the sterile line was always significantly lower than that of the maintainer line, while the CAT activity was significantly higher than that of the maintainer line.The POD activity was significantly lower than that of the maintainer line during the meiotic and tetrad period, and the MDA content was only significantly higher than maintainer in the mononuclear period.Content GA₃ and IAA in tetrad and mononuclear period in sterile line were higher than that of the maintainer, ZR content was always higher than that of the maintainer, JA content in meiosis stage was significantly lower than that of the maintainer, IAA content in tetrad period was significantly higher in the maintainer line.The change trends of IAA/ABA, IAA/GA₃, IAA/JA, IAA/ZR, ABA/GA₃, ABA/ZR between sterile line and the maintainer line were inconsistent.The ratio content of IAA/ABA was higher than those of other endogenous hormones.【Conclusion】 Therefore, it is speculated that the changes of the protective enzyme activity, the abnormalcontent of endogenous hormone and the change in the hormone ratio in sterile line may affect anther endogenous substances and pollen vitality in the three stages of meiosis, tetrad, and mononucleus, resulting in the development of pollen in the anthers of the male sterile line in croissant pepper is hindered and the cause of abortion is formed.

Key words: Capsicum annuum L.; male sterility; morphology; antioxidant; endogenous hormone

摘要：

【目的】分析辣椒(Capsicum annuum L.)雄性不育系的形态学及生理生化的特征,为研究羊角椒雄性不育的遗传机制提供理论参考。【方法】以羊角椒核雄性不育系pby-1和保持系PBY-1为材料,观察花蕾7个时期的发育情况及盛花期的花器官,测定盛花期花粉的生活力及减数分裂、四分体及单核3个时期花蕾的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和丙二醛(MDA)的含量,分析保护酶活性的变化,比较赤霉素(GA₃)、生长素(IAA)、玉米素核苷(ZR)、茉莉酸(JA)及脱落酸(ABA)含量差异及不同内源激素的平衡关系。【结果】与野生型PBY-1相比,不育系只有花器官结构与其不同,花药干瘪,花粉无生活力。在羊角椒花蕾发育的3个时期,不育系与可育系之间在保护酶活性与内源激素含量上存在差异,其中,不育系SOD活性始终显著低于保持系,而CAT活性始终显著高于保持系,POD活性在减数分裂期和四分体时期显著低于保持系,MDA含量只有在单核时期显著高于保持系;四分体及单核时期GA₃含量、IAA含量高于保持系,ZR含量始终高于保持系,减数分裂期JA含量明显低于保持系,四分体时期IAA含量明显高于保持系;不育系和保持系IAA/ABA、IAA/GA₃、IAA/JA、IAA/ZR、ABA/GA₃、ABA/ZR的变化趋势不一致,且IAA/ABA的比值高于其他内源激素含量的比值。【结论】不育系保护酶活性的变化和内源激素含量的异常以及激素比值的变化可能影响了减数分裂、四分体及单核3个时期的花药内源物质和花粉活力,导致羊角椒不育系花药中花粉发育受阻、形成败育。

关键词: 辣椒, 雄性不育, 形态学, 抗氧化物, 内源激素

CLC Number:

S641.3

PEI Hongxia, LI Shengmei, WU Xuxia, GENG Shiwei, LAI Lili, GAO Jingxia, DONG Xinjiu. Morphological Observation and Physio-Biochemical Characteristics of Hot Pepper Genic Male - Sterile Line pby-1[J]. Xinjiang Agricultural Sciences, 2023, 60(3): 624-632.

裴红霞, 李生梅, 武旭霞, 耿世伟, 赖黎丽, 高晶霞, 董心久. 辣椒核雄性不育系pby-1形态学及生理生化特性分析[J]. 新疆农业科学, 2023, 60(3): 624-632.

Figures/Tables 7

References 33

[1]	LIU C, Ma N, Wang P Y, et al. Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.)[J]. Plos One, 2013, 8(6):e65209. DOI URL
[2]	胥志文. 线辣椒杂交品种栽培技术[J]. 辣椒杂志, 2012, (2):22-23.
	XU Zhiwen. Cultivation techniques of hybrid pepper varieties[J]. Journal of China Capsicum, 2012,(2): 22-23.
[3]	耿三省, 王德欣, 陈斌. 等. 我国南菜北运基地辣椒品种市场需求变化趋势[J]. 中国蔬菜, 2015, 1(12):1-3.
	GENG Sanxing, WANG Dexin, CHEN Bin, et al. The market demand trend of pepper varieties in the north-south vegetable transportation base in my country[J]. China Vegetable, 2015, 1(12): 1-3.
[4]	侯杰, 韩玉珠, 张晓旭. 等. 不同播期对辣椒雄性不育生理生化特性的影响[J]. 东北农业科学, 2017, 42(1):46-49.
	HOU Jie, HAN Yuzhu, ZHANG Xiaoxu, et al. Effect of sowing date on physiological and biochemical of pepper male sterility lines[J]. Northeast Agricultural Sciences, 2017, 42(1): 46-49.
[5]	蒋会兵, 杨盛美, 刘玉飞. 等. 厚轴茶雄性不育株花药败育的生物学特性和细胞学研究[J]. 作物学报, 2020, 46(7):1076-1086. DOI
	JIANG Huibing, YANG Shengmei, LIU Yufei, et al. Biological characteristics and cytological studies on anther abortion of male sterile Camellia crassocolumna[J]. Acta Agronomica Sinica, 2020, 46(7): 1076-1086. DOI
[6]	谢潮添, 魏冬梅, 田惠桥. 高等植物雄性不育的细胞生物学研究进展[J]. 植物生理与分子生物学学报, 2006, 32(1):17-23. PMID
	XIE Chaotian, WEI Dongmei, TIAN Huiqiao. Advances in cell biological researches on male sterility of higher plants[J]. Journal of Plant Physiology and Molecular Biology, 2006, 32(1): 17-23. PMID
[7]	邓明华. 辣椒胞质雄性不育的分子生理机制及亲缘关系研究[D]. 长沙: 中南大学, 2011.
	DENG Minghua. Studies on molecular and physiological mechanisms of pepper cytoplasmic male sterility and geneticrelationship in capsicum[D]. Changsha: Central South University, 2011.
[8]	贾文杰, 郑洁, 崔光芬. 等. 百合育性与花药发育过程中激素和内源物质含量及能量代谢酶活性的关系[J]. 西北植物学报, 2019, 39(3):480-488.
	JIA Wenjie, ZHENG Jie, CUI Guangfen, et al. Relationship between Lilium fertility and hormone, endogenous substance content, energy metabolism enzyme activity during anther development[J]. Acta Botanica Boreali-Occidentalia Sinica, 2019, 39(3): 480-488.
[9]	姚雅琴, 张改生, 刘宏伟. 等. K型小麦花粉粒内壁及ATP酶活性与雄性不育的相关性[J]. 西北植物学报, 2002,(2):333-337.
	YAO Yaqin, ZHANG Gaisheng, LIU Hongwei, et al. Studies on correlation between intine and K-type cytoplasmic male sterility in wheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2002,(2): 333-337.
[10]	刘齐元, 程元强, 朱肖文. 等. 雄性不育烟草花蕾中SOD、POD和CAT活性研究[J]. 中国烟草学报, 2011, 17(5):34-39.
	LIU Qiyuan, CHENG Yuanqiang, ZHU Xiaowen, et al. Studies on SOD, POD and CAT activities in flower buds of tobacco of cytoplasmic male sterility[J]. Acta Tabacaria Sinica, 2011, 17(5): 34-39.
[11]	陈全求, 蓝家样, 韩光明. 等. 核不育系杂交棉不同发育时期抗氧化酶活性变化及其杂种优势分析[J]. 湖北农业科学, 2015, 54(24):6173-6177.
	CHEN Quanqiu, LAN Jiaxiang, HAN Guangmin, et al. Antioxidant enzyme activity change and heterosis analysis of hybrid cotton of nuclear sterile lines in the different growing stage[J]. Hubei Agricultural Sciences, 2015, 54(24): 6173-6177.
[12]	王朋宝, 王开芳, 张金文. 等. 甘蓝型油菜隐性核不育系花蕾生理生化特性研究[J]. 河北农业大学学报, 2017, 40(5):6-11.
	WANG Pengbao, WANG Kaifang, ZHANG Jinwen, et al. Physiological and biochemical characteristics studies on flower buds of recessive genetic male sterile line in Brassica napus[J]. Journal of Henan Agriculture University, 2017, 40(5): 6-11.
[13]	冯小磊, 范光宇, 苏旭. 等. 植物雄性不育生理生化研究进展[J]. 作物杂志, 2012,(3):6-11.
	FENG Xiaolei, FAN Guangyu, SU Xu, et al. Advances in physiological and biochemical study on plant male sterility[J]. Crops, 2012,(3): 6-11.
[14]	陶兴林, 朱惠霞, 胡立敏. 等. 花椰菜温敏雄性不育系GS-19花蕾发育过程中内源激素的动态变化分析[J]. 核农学报, 2017, 31(8):1626-1632. DOI
	TAO Xinglin, ZHU Huixia, HU Liming, et al. Dynamic analysis of endogenous hormones during bud developmentstage in cauliflower thermo-sensitive male sterile line GS-19[J]. Journal of Nuclear Agriculture Science, 2017, 31(8): 1626-1632.
[15]	盛云燕, 常薇, 矫士琦. 等. 甜瓜雄性不育植株雄蕊发育结构及生理生化特征[J]. 植物生理学报, 2016, 52(7):1028-1034.
	SHENG Yunyan, CHANG Wei, JIAO Shiqi, et al. Stamen structure development and physiological and biochemical characteristics in male sterile melon[J]. Plant Physiology Journal, 2016, 52(7): 1028-1034.
[16]	李玉青, 王清连, 韦春艳. 等. 棉花胞质雄性不育细胞形态学观察及生理生化特性的研究[J]. 西南农业学报, 2020, 33(1):58-63.
	LI Yuqing, WANG Qinglian, WEI Chunyan, et al. Morphological observation and physiological and biochemical characteristics of cotton cytoplasmic male sterile cells[J]. Southwest China Journal Agriculture Sciences, 2020, 33(1): 58-63.
[17]	蒋会兵, 许燕, 宋维希. 等. 厚轴茶雄性不育株花蕾发育过程中的生理生化变化[J]. 植物生理学报, 2020, 56(9):1807-1817.
	JIANG Huibing, XU Yan, SONG Weixi, et al. Physiological and biochemical changes during bud development inmale sterile plant of Camellia crassicolumna[J]. Journal of Plant Physiolgy, 2020, 56(9): 1807-1817.
[18]	王永琦, 杨小振, 莫言玲. 等. 西瓜雄性不育系‘Se18’抗氧化酶活性和内源激素含量变化分析[J]. 园艺学报, 2016, 43(11):2161-2172. DOI
	WANG Yongqi, YANG Xiaozhen, MO Yanling, et al. Analysis of the changes in antioxidant enzymes cctivities and endogenous hormones contents in watermelon male sterile line Se18 during bud development[J]. Acta Horticulture Sinica, 2016, 43(11):2161-2172.
[19]	张菊平, 巩振辉, 刘珂珂. 等. 辣椒小孢子发育时期与花器形态的相关性[J]. 西北农林科技大学学报(自然科学版), 2007,(3):153-158.
	ZHANG Juping, GONG Zhenhui, LIU Keke, et al. Interrelation of cytological development period of pepper microspore and the morphology of flower organ[J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Ed.), 2007,(3):153-158.
[20]	黄巍, 缪武. 两种辣椒雄性不育系主要农艺性状对比分析[J]. 辣椒杂志, 2017, 15(3):21-26.
	HUANG Wei, MIU Wu. Comparison on agronomic traits of two types of male sterile lines in hot pepper[J]. Journal of China Capsicum, 2017, 15(3):21-26.
[21]	黄炜. 辣椒核雄性不育两用系创制及其不育机理研究[D]. 杨凌: 西北农林科技大学, 2012.
	HUANG Wei. Breeding of genic male sterile line and studies on sterile mechanism in pepper[D]. Yangling: Northwest A&F University, 2012.
[22]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI Hesheng. Principles and techniques of plant physiology and biochemistry experiment[M]. Beijing: Higher Education Press, 2000.
[23]	易斌, 涂金星, 傅廷栋. 甘蓝型油菜隐性细胞核雄性不育的研究及利用[J]. 中国科学:生命科学, 2014, 44(8):752-757.
	YI Bin, TU Jinxing, FU Tingdong. Recessive genic male sterility in Brassica napus and its application[J]. Scientia Sinica Vitae, 2014, 44(8): 752-757. DOI URL
[24]	Jacobowitz Josephr, Doyle Williamc, Weng J K. PRX9 and PRX40 are extensin peroxidases essential for maintaining tapetum and microspore cell wall integrity during arabidopsis anther development[J]. Plant Cell, 2019, 31:848-861. DOI
[25]	LIU Zihan, SHI Xiaoyi, LI Sha, et al. Tapetal-delayed programmed cell death (PCD) and oxidative stress-induced male sterility of aegilops uniaristata cytoplasm in wheat[J]. International Journal of Molecular Sciences, 2018, 19(6):1708. DOI URL
[26]	许代香, 贾乐东, 王瑞. 等. 甘蓝型油菜显性核不育系D3A的细胞学研究[J]. 西南大学学报(自然科学版), 2020, 42(1):22-27.
	XU Daixiang, JIA Ledong, WANG Rui, et al. Cytological studies of dominant GMS sterile line D3A in Brassica napus L.[J]. Journal of Southwest University (Natural Science Ed.), 2020, 42(1): 22-27.
[27]	XIE Xianrong, ZHANG Zixu, ZHAO Zhe, et al. The mitochondrial aldehyde dehydrogenase OsALDH2b negatively regulates tapetum degeneration in rice[J]. Journal of Experimental Botany, 2020, 71(9):2551-2560. DOI PMID
[28]	张锐, 尚伟, 许旭明. 辣椒雄性不育的选育及利用研究进展[J]. 分子植物育种, 2020, 18(18):6143-6157.
	ZHANG Rui, SHANG Wei, XU Xuming. Research progress on breeding and application of male sterility in hot pepper[J]. Molecular Plant Breeding, 2020, 18(18): 6143-6157.
[29]	Sawheny Vipenk, Shukla Amit. Male sterility in flowering plants: are plant growth substances involved[J]. American Journal of Botany, 1994, 81(12):1640-1647. DOI URL
[30]	Tang R S, Zheng J C, Jin Z Q, et al. Possible correlation between high temperature-induced floret sterility and endogenous levels of IAA, GAs and ABA in rice (Oryza sativa L.)[J]. Plant Growth Regul., 2008, 54(1):37-43. DOI URL
[31]	刘红艳, 吴坤, 杨敏敏. 等. 芝麻显性细胞核雄性不育系内源激素、可溶性糖和淀粉含量变化[J]. 中国油料作物学报, 2014, 36(2):175-180.
	LIU Hongyan, WU Kun, YANG Minmin, et al. Variation of soluble sugar, starch and plant hormones contents in sesamedominant genic male sterile line during bud development[J]. Chinese Journal of Oil Crops, 2014, 36(2): 175-180.
[32]	许小勇, 张静, 孙希禄. 等. 大白菜CMS7311雄性不育的发生与花蕾内源激素含量变化的关系研究[J]. 华北农学报, 2014,(6):180-185.
	XU Xiaoyong, ZHANG Jing, SUN Xilu, et al. The relationship between male sterile occurrence and dynamics of endogenous hormone contents during flower bud development in CMS7311 of heading Chinese cabbage[J]. Acta Agriculture Boreali-Sinica, 2014,(6): 180-185.
[33]	吴智明, 胡开林, 符积钦. 等. 辣椒胞质雄性不育与花蕾内源激素含量的关系[J]. 华南农业大学学报, 2010, 31(2):1-4.
	WU Zhiming, HU Kailin, FU Jikan, et al. Relationships between cytoplasmic male sterility and endogenous hormone content of pepper bud[J]. Journal of South China Agriculture Univrsity, 2010, 31(2): 1-4.

材料 Materials	小孢子发育时期 Microspore development time	花蕾形态特征 Morphological characteristics flower buds	花蕾纵横径 Veritical and horizontal diameter of flower bud
材料 Materials	小孢子发育时期 Microspore development time	花蕾形态特征 Morphological characteristics flower buds	纵径 Longitudinal diameter(mm)	横径 Transverse diameter(mm)
pby-1	S₁	花蕾小,花萼紧裹花冠,深绿色。	2.49~2.98	2.07~2.63
	S₂	花蕾比S₁期花蕾微大,花萼裹着花冠,深绿色。	2.99~4.49	2.64~3.59
	S₃	花萼上端微张开,深绿色,花冠绿色,花冠与花萼齐平。	4.50~5.87	3.60~4.10
	S₄	花萼上端张开,深绿色,花冠绿色,花冠约占花萼长的1/4。	5.88~6.52	4.11~4.86
	S₅	花冠绿色,伸出花萼部分的长度短于花萼,约占花萼长的1/2。	6.53~7.23	4.87~5.54
	S₆	花冠浅绿色,伸出花萼部分的长度约与花萼等长。	7.22~8.89	5.55~5.90
	S₇	花冠白绿色,大小充分膨大。	8.90~11.98	5.91~6.40
PBY-1	F₁	花蕾小,花萼紧裹花冠,深绿色。	2.57~2.93	2.15~2.84
	F₂	花蕾比F₁期花蕾微大,花萼裹着花冠,深绿色。	2.94~4.81	2.85~3.73
	F₃	花萼上端微张开,深绿色,花冠绿色,花冠与花萼齐平。	4.82~5.75	3.72~4.08
	F₄	花萼上端张开,深绿色,花冠绿色,花冠约占花萼长的1/4。	5.76~6.66	4.09~4.94
	F₅	花冠绿色,伸出花萼部分的长度短于花萼,约占花萼长的1/2。	6.67~7.28	4.95~5.76
	F₆	花冠浅绿色,伸出花萼部分的长度约与花萼等长。	7.29~8.08	5.77~5.91
	F₇	花冠白绿色,大小充分膨大。	8.09~11.84	5.92~6.80

材料 Materials	小孢子发育时期 Microspore development time	花蕾形态特征 Morphological characteristics flower buds	花蕾纵横径 Veritical and horizontal diameter of flower bud
材料 Materials	小孢子发育时期 Microspore development time	花蕾形态特征 Morphological characteristics flower buds	纵径 Longitudinal diameter(mm)	横径 Transverse diameter(mm)
pby-1	S₁	花蕾小,花萼紧裹花冠,深绿色。	2.49~2.98	2.07~2.63
	S₂	花蕾比S₁期花蕾微大,花萼裹着花冠,深绿色。	2.99~4.49	2.64~3.59
	S₃	花萼上端微张开,深绿色,花冠绿色,花冠与花萼齐平。	4.50~5.87	3.60~4.10
	S₄	花萼上端张开,深绿色,花冠绿色,花冠约占花萼长的1/4。	5.88~6.52	4.11~4.86
	S₅	花冠绿色,伸出花萼部分的长度短于花萼,约占花萼长的1/2。	6.53~7.23	4.87~5.54
	S₆	花冠浅绿色,伸出花萼部分的长度约与花萼等长。	7.22~8.89	5.55~5.90
	S₇	花冠白绿色,大小充分膨大。	8.90~11.98	5.91~6.40
PBY-1	F₁	花蕾小,花萼紧裹花冠,深绿色。	2.57~2.93	2.15~2.84
	F₂	花蕾比F₁期花蕾微大,花萼裹着花冠,深绿色。	2.94~4.81	2.85~3.73
	F₃	花萼上端微张开,深绿色,花冠绿色,花冠与花萼齐平。	4.82~5.75	3.72~4.08
	F₄	花萼上端张开,深绿色,花冠绿色,花冠约占花萼长的1/4。	5.76~6.66	4.09~4.94
	F₅	花冠绿色,伸出花萼部分的长度短于花萼,约占花萼长的1/2。	6.67~7.28	4.95~5.76
	F₆	花冠浅绿色,伸出花萼部分的长度约与花萼等长。	7.29~8.08	5.77~5.91
	F₇	花冠白绿色,大小充分膨大。	8.09~11.84	5.92~6.80

材料 Material	发育时期 Bud stage	IAA/ABA	IAA/GA₃	IAA/JA	IAA/ZR	ABA/GA₃	ABA/ZR
pby-1	减数分裂	2.20	2.46	0.54	0.89	1.12	0.41
	四分体时期	3.95	1.29	0.45	0.68	0.33	0.17
	单核时期	3.36	1.44	0.37	0.64	1.43	0.19
PBY-1	减数分裂	4.11	1.28	0.29	1.30	0.31	0.32
	四分体时期	1.51	1.11	0.35	0.69	0.74	0.45
	单核时期	2.84	1.27	0.21	0.45	0.45	0.16

材料 Material	发育时期 Bud stage	IAA/ABA	IAA/GA₃	IAA/JA	IAA/ZR	ABA/GA₃	ABA/ZR
pby-1	减数分裂	2.20	2.46	0.54	0.89	1.12	0.41
	四分体时期	3.95	1.29	0.45	0.68	0.33	0.17
	单核时期	3.36	1.44	0.37	0.64	1.43	0.19
PBY-1	减数分裂	4.11	1.28	0.29	1.30	0.31	0.32
	四分体时期	1.51	1.11	0.35	0.69	0.74	0.45
	单核时期	2.84	1.27	0.21	0.45	0.45	0.16

Morphological Observation and Physio-Biochemical Characteristics of Hot Pepper Genic Male - Sterile Line pby-1

辣椒核雄性不育系pby-1形态学及生理生化特性分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 7

References 33

Related Articles 15

Recommended Articles 0

Metrics

[1]	LI Yongtai, GAO Axiang, LI Yanjun, ZHANG Xinyu. Effects of defoliants on the physiological characteristics of cotton varieties with different sensitivities [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2094-2102.
[2]	Arezigu Tuxun, JIA Kai, GAO Jie. Effects of different substrates and planting densities on onion bulblet yield [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1993-2003.
[3]	HU Jinge, BAI Shijian, CHEN Guang, CAI Junshe. Effects of different ground mulch types on the berry quality of Marselan wine grape and comprehensive evaluation [J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1131-1139.
[4]	YANG Wenli, XU Lirong, LIU Bin, LING Yueming, LI Meihua, YANG Yong, FAN Rong, LI Yushun, ZHANG Yongbin, ZHANG Xuejun. Effects of salt stress on ion balance, membrane lipid peroxidation, and osmotic regulation substance accumulation in thin skin muskmelon ‘Huishu’ [J]. Xinjiang Agricultural Sciences, 2024, 61(4): 900-907.
[5]	Gulidan Sailikebole, WANG Chengxin, XU Hao, HU Bolin, YANG Haoke, WEI Qi, CHEN Shengao. Research on the biology of Triplophysa strauchiiin Turks River [J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2576-2582.
[6]	LIU Jiajia, HAN Tao. Effect of pomegranate peel flesh extract on body weight gain and plasma Immunity-related indexes in adult mice [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2074-2080.
[7]	BAI Ling, FENG Guojun, HU Xiangwei, ZHAO Yun, SHI Shubing. Drought resistance identification and physiological changes of different millet varieties during germination [J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1630-1640.
[8]	LUO Siwei, JIA Yonghong, ZHANG Jinshan, WANG Kai, LI Dandan, WANG Runqi, DONG Yanxue, SHI Shubing. Effects of drip irrigation capillary spacing and emitter spacing on the spatial distribution of soil water, root morphology and yield of uniformly sown winter wheat [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1344-1352.
[9]	YANG Mengqi, NAN Shanshan, ZHANG Xiaoyang, WANG Hailiang, LI Jiacheng, NIU Junli, NIE Cunxi, ZHANG Wenju. Effects of fermented cotton meal fiber on growth performance, slaughter performance and intestinal morphology of yellow feather broilers [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 1011-1019.
[10]	CHEN Liliang, LU Qianjun, MA Yuanyuan, LIU Ying, ZHAO Baolong, SUN Junli. Effects of NaCl stress on antioxidant enzyme content and malondialdehyde content in grape cultivars leaves [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 880-888.
[11]	LI Shuang, XIE Danlu, WU Jizhou, MA Rui, XIE Aidi, SHAO Lei, GAO Juanjuan, HAN Haixia. Comparison of main functional components and antioxidant activity of jujube Ruoqiang by different processing methods [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 935-942.
[12]	LIU Wenhao, JIN Guili, WU Xueer, GONG Ke, HAN Wanqiang. Seed Morphology and Germination Characteristics of Bromus inermis at Different Seed Shattering Times [J]. Xinjiang Agricultural Sciences, 2023, 60(3): 727-734.
[13]	HOU Yurong, KE Mei, LI Chao, LAN Jiyong, KANG Shuai, JIN Junpeng, WEI Peng. Effects of fencing and free grazing on growth patterns and endogenous hormones of Seriphidium transiliense [J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2824-2832.
[14]	Zulipiya Maimaiti, MAO Hongyan, YUE Li, WANG Jiamin, YU Ming. Study on in vitro antioxidant activities and digestive characteristics of tricolor quinoa [J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2461-2469.
[15]	ZHENG Zipiao, XU Haijiang, CUI Jianping, LIN Tao, GUO Rensong, WANG Liang, ZHANG Dawei, WEI Xin, KONG fanyang. Effects of Water Stress on Growth and Development in Cotton （Gossypium hirsutum L.） [J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1821-1830.