Drought resistance identification and physiological changes of different millet varieties during germination

doi:10.6048/j.issn.1001-4330.2023.07.009

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (7): 1630-1640.DOI: 10.6048/j.issn.1001-4330.2023.07.009

• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles

Drought resistance identification and physiological changes of different millet varieties during germination

BAI Ling¹(), FENG Guojun²(), HU Xiangwei², ZHAO Yun², SHI Shubing¹()

1. College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Food Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2022-11-10 Online:2023-07-20 Published:2023-07-11
Correspondence author: FENG Guojun (1970-), female, born in Qitai, Xinjiang, researcher, doctor, postgraduate supervisor, research direction:Miscellaneous grain breeding and cultivation,(E-mail)fengguojxj@126.com；
SHI Shubing (1966-), male, Shandong native, professor, doctoral supervisor, research direction for high quality and high yield cultivation of wheat, (E-mail)shubshi@sina.com
Supported by:
National Natural Science Foundation:precise identification of drought resistance of millet germplasm resources and excavation of drought-resistant genes(3206150401);National Millet and Sorghum Industrial Technology System Urumqi Comprehensive Experiment Station(CARS-06-14.5-B30)

不同谷子品种萌发期抗旱鉴定及生理变化

柏玲¹(), 冯国郡²(), 胡相伟², 赵云², 石书兵¹()

1.新疆农业大学农学院,乌鲁木齐 830052
2.新疆农业科学院粮食作物研究所,乌鲁木齐 830091

通讯作者: 冯国郡(1970-),女,新疆奇台人,研究员,博士,硕士生导师,研究方向为杂粮育种及栽培,(E-mail)fengguojxj@126.com；
石书兵(1966-),男,山东人,教授,硕士生/博士生导师,研究方向为小麦优质高产栽培,(E-mail)shubshi@sina.com
作者简介:柏玲(1996-),女,山东泰安人,硕士研究生,研究方向为谷子抗旱性,(E-mail)2503685718@qq.com
基金资助:
国家自然科学基金项目“谷子种质资源抗旱性精准鉴定及耐旱相关基因的挖掘”(3206150401);国家谷子高粱产业技术体系乌鲁木齐综合试验站(CARS-06-14.5-B30)

Abstract

Abstract:

【Objective】 To explore the impact of drought on millet germination, screen out drought resistance related indicators during millet germination, and further clarify the response mechanism of antioxidant enzymes in different millet varieties to drought stress during germination.the research results can provide a reference for selecting and cultivating millet varieties suitable for planting in Xinjiang.【Methods】 Using 20% polyethylene glycol (PEG-6000) to simulate drought stress during germination, the drought resistance of 15 millet varieties was evaluated, and the changes in the activities of SOD, POD, CAT, and GR of 15 varieties under drought stress were measured to study their internal situation. 【Results】 (1) Under drought stress, the germination potential, germination rate, radicle length, plumule length, fresh weight, and dry weight of the tested millet varieties were inhibited to varying degrees, with a relatively small impact on the germination rate, but the root buds of several varieties such as Mengfenggu 7 had a promoting effect.(2) A comprehensive evaluation of drought resistance was conducted using the membership function method. The drought resistance of 15 millet varieties was graded, including 5 strong drought resistant varieties, 4 moderate drought resistant varieties, 5 drought sensitive varieties, and 1 drought extremely sensitive type. (3) Under drought stress, the activities of antioxidant enzymes SOD, POD, CAT, and GR in millet seedlings of different varieties increased, and the drought tolerant varieties themselves had relatively high antioxidant enzyme activities and could maintain high levels under drought stress. 【Conclusion】 Overall, Mengfenggu 7 has the best drought resistancehe.

Key words: millet; PEG; antioxidant enzyme; membership function value

摘要：

【目的】研究出谷子萌发期抗旱性相关指标,分析不同谷子品种萌发期内在抗氧化酶对干旱胁迫的响应机理,为筛选和培育适宜新疆地区种植的谷子品种提供参考。【方法】用20%的聚乙二醇(PEG-6000)模拟萌发期干旱胁迫对15个谷子品种进行抗旱性鉴定,测定15个品种在干旱胁迫条件下SOD、POD、CAT和GR的活性。【结果】(1)干旱胁迫下,受试品种谷子的发芽势、发芽率、胚根长、胚芽长、芽鲜重、芽干重均受到不同程度的抑制,对发芽率的影响相对较小,但蒙丰谷7号等几个品种的根芽具有促进作用。(2)用隶属函数法进行抗旱性综合评价,将15个谷子品种的抗旱性划分等级,其中强抗旱型品种5个、中度抗旱型品种4个、干旱敏感型品种5个、干旱极敏感型1个。(3)干旱胁迫下不同品种谷子幼苗抗氧化酶SOD、POD、CAT、GR活性均有所上升,且耐旱性品种本身抗氧化酶活性就比较高且在干旱胁迫下维持较高水平。【结论】蒙丰谷7号的抗旱性最好。

关键词: 谷子, PEG, 抗氧化酶, 隶属函数值

CLC Number:

S515

BAI Ling, FENG Guojun, HU Xiangwei, ZHAO Yun, SHI Shubing. Drought resistance identification and physiological changes of different millet varieties during germination[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1630-1640.

柏玲, 冯国郡, 胡相伟, 赵云, 石书兵. 不同谷子品种萌发期抗旱鉴定及生理变化[J]. 新疆农业科学, 2023, 60(7): 1630-1640.

Figures/Tables 9

References 28

[1]	李斌, 陈满霞, 马萌萌, 等. 沿海地区氮肥形态对藜麦生长和单株籽粒干质量的影响[J]. 江苏农业科学, 2021, 49(23):87-93.
	LI Bin, CHEN Manxia, MA Mengmeng, et al. Effects of nitrogen fertilizer forms on the growth and dry grain quality of quinoa in coastal areas[J]. Jiangsu Agricultural Science, 2021, 49(23):87-93.
[2]	樊瑀, 董淑琦, 原向阳, 等. 谷子种质资源萌发期抗旱性综合评价及抗旱指标筛选[J]. 中国农业大学学报, 2022, 27(6):42-54.
	FAN Yu, DONG Shuqi, YUAN Xiangyang, et al. Comprehensive evaluation of drought resistance and screening of drought resistance indicators of millet germplasm resources during germination[J]. Journal of China Agricultural University, 2022, 27(6):42-54.
[3]	张犇, 郭悦, 刘丽文, 等. 控水干旱及PEG模拟干旱胁迫对谷子生理生化指标和SiVamp7基因转录水平的影响[J]. 中国生物化学与分子生物学报, 2023, 39(1):96-107.
	ZHANG Ben, GUO Yue, LIU Liwen, et al. Effects of water control drought and PEG simulated drought stress on physiological and biochemical indexes and SiVamp7 gene transcription level of millet[J]. Chinese Journal of Biochemistry and Molecular Biology, 2023, 39(1):96-107.
[4]	贾凯旋, 魏俊杰, 冯梦凡, 等. 河北省主要推广玉米品种萌发期抗旱性鉴定和生理性状分析[J]. 种子, 2020, 39(8):76-80.
	JIA Kaixuan, WEI Junjie, FENG Mengfan, et al. Identification of drought resistance and analysis of physiological characteristics of main maize varieties popularized in Hebei Province during germination[J]. Seed, 2020, 39 (8):76-80.
[5]	王振华, 刘鑫, 余爱丽, 等. 不同谷子品种萌发期对干旱胁迫生理响应的变化及抗旱指标筛选[J]. 中国农业科技导报, 2020, 22(12):39-49. DOI
	WANG Zhenhua, LIU Xin, YU Aili, et al. Changes in physiological response of different millet varieties to drought stress during germination and screening of drought resistance indicators[J]. China Journal of Agricultural Science and Technology, 2020, 22(12):39-49.
[6]	熊雪, 郎万鑫, 王春芳, 等. 不同谷子品种萌发期抗旱性综合评价[J]. 江苏农业科学, 2021, 49(23):93-97.
	XIONG Xue, LANG Wanxin, WANG Chunfang, et al. Comprehensive evaluation of drought resistance of different millet varieties during germination[J]. Jiangsu Agricultural Science, 2021, 49(23):93-97.
[7]	高汝勇, 时丽冉, 崔兴国, 等. 谷子品种抗旱性评价[J]. 河南农业科学, 2013, 42(12):28-32.
	GAO Ruyong, SHI Liran, CUI Xingguo, et al. Evaluation of drought resistance of millet varieties[J]. Henan Agricultural Science, 2013, 42(12):28-32.
[8]	朱永波, 张仁和, 卜令铎, 等. 玉米苗期抗旱性鉴定指标的研究[J]. 西北农业学报, 2008,(3):143-146.
	ZHU Yongbo, ZHANG Renhe, BU Lingduo, et al. Study on identification index of drought resistance in maize seedling stage[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, (3):143-146.
[9]	张雁明, 刘晓东, 马建萍, 等. 谷子抗旱研究进展[J]. 山西农业科学, 2013, 41(3):282-285.
	ZHANG Yanming, LIU Xiaodong, MA Jianping, et al. Research progress of millet drought resistance[J]. Shanxi Agricultural Science, 2013, 41(3):282-285.
[10]	崔纪菡, 范佳兴, 李顺国, 等. 谷子抗旱性鉴定研究进展[J]. 东北农业大学学报, 2017, 41(1):89-96.
	CUI Jihan, FAN Jiaxing, LI Shunguo, et al. Research progress in identification of drought resistance of millet[J]. Journal of Northeast Agricultural University, 2017, 41(1):89-96.
[11]	秦岭, 陈二影, 杨延兵, 等. 干旱和复水对不同耐旱型谷子品种苗期生理指标的影响[J]. 中国农业科技导报, 2019, 21(3):146-151. DOI
	QIN Ling, CHEN Erying, YANG Yanbing, et al. Effects of drought and rehydration on physiological indicators of different drought tolerant millet varieties at seedling stage[J]. China Journal of Agricultural Science and Technology, 2019, 21(3):146-151.
[12]	李云, 杨梦涵, 王健, 等. PEG胁迫下谷子品种(系)萌发期耐旱性鉴定及评价[J]. 种子, 2022, 41(4):29-35,43.
	LI Yun, YANG Menghan, WANG Jian, et al. Identification and evaluation of drought tolerance of millet varieties (lines) during germination under PEG stress[J]. Seed, 2022, 41(4):29-35,43.
[13]	代小冬, 杨育峰, 朱灿灿, 等. 谷子萌芽期对干旱胁迫的响应及抗旱性评价[J]. 华北农学报, 2015, 30(4):139-144. DOI
	DAI Xiaodong, YANG Yufeng, ZHU Cancan, et al. The response of millet to drought stress and the evaluation of drought resistance at the germination stage[J]. Journal of North China Agriculture, 2015, 30(4):139-144.
[14]	任毅, 颜安, 张芳, 等. 国内外301份小麦品种(系)种子萌发期抗旱性鉴定及评价[J]. 干旱地区农业研究, 2019, 37(3):1-14.
	REN Yi, YAN An, ZHANG Fang, et al. Identification and evaluation of drought resistance of 301 wheat varieties (lines) at seed germination stage at home and abroad[J]. Agricultural Research in Arid Areas, 2019, 37 (3):1-14.
[15]	张耀元, 张彬, 马芳芳, 等. 不同品种谷子幼苗对干旱胁迫的生理响应及SiZFP252基因的表达分析[J]. 山西农业大学学报(自然科学版), 2016, 36(9):614-618.
	ZHANG Yaoyuan, ZHANG Bin, MA Fangfang, et al. Physiological response of millet seedlings of different varieties to drought stress and expression analysis of SiZFP252 gene[J]. Journal of Shanxi Agricultural University (Natural Science ), 2016, 36 (9):614-618.
[16]	张加利, 张雨欣, 王宁, 等. PEG预处理对文冠果种子萌发和幼苗生长的影响[J]. 林业与生态科学, 2022, 37(4):384-389.
	ZHANG Jiali, ZHANG Yuxin, WANG Ning, et al. Effects of PEG pretreatment on seed germination and seedling growth of Xanthoceras sorbifolia[J]. Forestry and Ecological Sciences, 2022, 37(4):384-389.
[17]	Karlaj M, Marcela A, Rafael V D. Antioxidant capacity of poly(Ethylene Glycol) (PEG) as protection mechanism a-gainst hydrogen peroxide inactivation of peroxidases[J]. Applied Biochemistry &Biotechnology, 2015, 177(6):1364-1373.
[18]	Tang D F, Wei F, Qin S X, et al. Polyethylene glycol induced drought stress strongly influences seed germination,root mor-phology and cytoplasm of different kenaf genotypes[J]. Industrial Crops &Products, 2019, 137:180-186.
[19]	王星宇, 王霞, 程静, 等. 冀北不同裸燕麦品种萌芽期抗旱性评价[J]. 山西农业科学, 2023, 51(2):133-142.
	WANG Xingyu, WANG Xia, CHENG Jing, et al. Evaluation of drought resistance of different naked oat varieties in northern Hebei during germination[J]. Shanxi Agricultural Science, 2023, 51(2):133-142.
[20]	吴秀宁, 敬樊, 张军, 王新军, 等. PEG-6000模拟干旱胁迫对黑小麦种子萌发特性的影响[J]. 作物研究, 2022, 36(4):307-312.
	WU Xiuning, JING Fan, ZHANG Jun, Wang Xinjun, et al. Effects of PEG-6000 simulated drought stress on seed germination characteristics of black wheat[J]. Crop Research, 2022, 36(4):307-312.
[21]	张晓丽, 陶伟, 陈雷, 等. 基于隶属函数值法的直播稻芽期和幼苗期耐低温淹水能力综合评价[J]. 南方农业学报, 2021, 52(1):78-85.
	ZHANG Xiaoli, TAO Wei, CHEN Lei, et al. Comprehensive evaluation of low temperature and waterlogging resistance of direct seeding rice at the bud and seedling stages based on the membership function value method[J]. Journal of Southern Agriculture, 2021, 52(1):78-85.
[22]	裴帅帅, 尹美强, 温银元, 等. 不同品种谷子种子萌发期对干旱胁迫的生理响应及其抗旱性评价[J]. 核农学报, 2014, 28(10):1897-1904. DOI
	PEI Shuaihuai, YIN Meiqiang, WEN Yinyuan, et al. Physiological response of different millet varieties to drought stress during seed germination and evaluation of drought resistance[J]. Journal of Nuclear Agriculture, 2014, 28 (10):1897-1904.
[23]	王军, 周美学, 许如根, 等. 大麦耐湿性鉴定指标和评价方法研究[J]. 中国农业科学, 2007, 40(10):2145-2152.
	WANG Jun, ZHOU Meimei, XU Rugen, et al. Study on identification indicators and evaluation methods of barley moisture tolerance[J]. Scientia Agricultura Sinica, 2007, 40 (10):2145-2152.
[24]	王萌, 赵曾菁, 赵虎, 等. 基于隶属函数和聚类分析法的广西韭菜地方种质资源耐热性评价[J/OL]. 西南农业学报:1-12[2023-03-04].
	WANG Meng, ZHAO Zengjing, ZHAO Hu, et al. Evaluation of heat tolerance of local Chinese chive germplasm resources in Guangxi based on membership function and cluster analysis[J/OL]. Southwest China Journal of Agricultural Sciences:1- 12 [23-03-04].
[25]	许健, 于海林, 王宇先, 等. 苗期玉米对PEG-6000胁迫的生理响应[J]. 黑龙江农业科学, 2021,(8):8-11.
	XU Jian, YU Hailin, WANG Yuxian, et al. Physiological response of maize seedlings to PEG-6000 stress[J]. Heilongjiang Agricultural Science, 2021, (8):8-11.
[26]	冯坤, 郑青松, 俞佳虹, 等. 超氧化物歧化酶的遗传特征及其在植物抗逆性中的研究进展[J]. 分子植物育种, 2017, 15(11):4498-4505.
	FENG Kun, ZHENG Qingsong, YU Jiahong, et al. The genetic characteristics of superoxide dismutase and its research progress in plant stress resistance[J]. Molecular Plant Breeding, 2017, 15(11):4498-4505.
[27]	秦立刚, 李雪, 李韦瑶, 等. PEG干旱胁迫对3种葱属植物种子萌发期渗透调节物质及酶活性的影响[J]. 草地学报, 2021, 19(1):72-79.
	QIN Ligang, LI Xue, LI Weiyao, et al. Effects of PEG drought stress on osmotic regulators and enzyme activities of three Allium species during seed germination[J]. Acta Agrestia Sinica, 2021, 19(1):72-79.
[28]	麦苗苗, 石大兴, 王米力, 等. PEG处理对连香树种子萌发与芽苗生长的影响[J]. 林业科学, 2009, 45(10):94-99.
	MAIMiamiao, SHI Daxing, WANG Mili, et al. Effects of PEG treatment on seed germination and seedling growth of Pistacia chinensis[J]. Scientia Silvae Sinicae, 2009, 45(10):94-99.

编号 No.	名称 Name	来源 Origin
1	九谷33	吉林
2	龙32	黑龙江
3	蒙丰谷7号	内蒙古
4	济谷12	山东
5	龙谷28	黑龙江
6	豫谷23	河南
7	陇谷17	甘肃
8	公谷67号	吉林
9	张杂谷19号	河北
10	制1-16H	北京
11	冀谷19	河北
12	九谷11	吉林
13	坝谷214	河北
14	夏谷2号	山西
15	13H616	北京

编号 No.	名称 Name	来源 Origin
1	九谷33	吉林
2	龙32	黑龙江
3	蒙丰谷7号	内蒙古
4	济谷12	山东
5	龙谷28	黑龙江
6	豫谷23	河南
7	陇谷17	甘肃
8	公谷67号	吉林
9	张杂谷19号	河北
10	制1-16H	北京
11	冀谷19	河北
12	九谷11	吉林
13	坝谷214	河北
14	夏谷2号	山西
15	13H616	北京

品种 Variety	发芽势Germination potential			发芽率Germination percentage
品种 Variety	CK(%)	20%PEG(%)	降幅(%)	CK(%)	20%PEG(%)	降幅(%)
1	68.00±1.16	52.00±1.16^**	23.53	76.00±0.00	60.00±2.31^**	21.05
2	48.00±1.16	44.00±1.16	8.33	64.00±2.31	57.00±1.16	10.94
3	95.00±0.58	60.00±1.16^**	36.84	94.67±0.96	85.33±0.77^*	9.87
4	71.00±1.73	40.00±2.31^**	43.66	90.66±0.20	90.07±0.04	0.65
5	44.00±1.16	11.00±0.58^**	75.00	50.67±0.97	38.67±1.35^**	23.68
6	79.00±1.54	75.00±0.89	5.06	88.00±1.35	78.67±0.96^**	10.60
7	55.00±0.58	39.00±0.58^**	29.09	61.33±0.77	60.00±0.16	2.17
8	92.00±1.54	87.00±0.77	5.44	96.00±1.16	90.00±1.16	6.25
9	83.00±1.91	82.00±1.36	1.21	90.67±1.04	90.00±1.36	0.74
10	89.00±1.54	64.00±1.54^**	28.09	92.00±1.55	81.33±1.29^**	11.60
11	77.00±1.73	35.00±0.58^**	54.55	85.33±0.47	78.67±0.91^**	7.80
12	60.00±1.16	47.00±0.16^**	21.67	96.00±1.16	94.56±1.14	1.50
13	75.00±1.87	68.00±1.48	9.32	94.67±0.63	76.00±3.59^**	19.72
14	71.00±1.07	61.00±1.91^**	14.09	76.00±1.42	68.00±1.55^*	10.53
15	68.00±2.31	45.00±0.58^**	33.82	96.00±1.16	89.00±2.89	7.29

品种 Variety	发芽势Germination potential			发芽率Germination percentage
品种 Variety	CK(%)	20%PEG(%)	降幅(%)	CK(%)	20%PEG(%)	降幅(%)
1	68.00±1.16	52.00±1.16^**	23.53	76.00±0.00	60.00±2.31^**	21.05
2	48.00±1.16	44.00±1.16	8.33	64.00±2.31	57.00±1.16	10.94
3	95.00±0.58	60.00±1.16^**	36.84	94.67±0.96	85.33±0.77^*	9.87
4	71.00±1.73	40.00±2.31^**	43.66	90.66±0.20	90.07±0.04	0.65
5	44.00±1.16	11.00±0.58^**	75.00	50.67±0.97	38.67±1.35^**	23.68
6	79.00±1.54	75.00±0.89	5.06	88.00±1.35	78.67±0.96^**	10.60
7	55.00±0.58	39.00±0.58^**	29.09	61.33±0.77	60.00±0.16	2.17
8	92.00±1.54	87.00±0.77	5.44	96.00±1.16	90.00±1.16	6.25
9	83.00±1.91	82.00±1.36	1.21	90.67±1.04	90.00±1.36	0.74
10	89.00±1.54	64.00±1.54^**	28.09	92.00±1.55	81.33±1.29^**	11.60
11	77.00±1.73	35.00±0.58^**	54.55	85.33±0.47	78.67±0.91^**	7.80
12	60.00±1.16	47.00±0.16^**	21.67	96.00±1.16	94.56±1.14	1.50
13	75.00±1.87	68.00±1.48	9.32	94.67±0.63	76.00±3.59^**	19.72
14	71.00±1.07	61.00±1.91^**	14.09	76.00±1.42	68.00±1.55^*	10.53
15	68.00±2.31	45.00±0.58^**	33.82	96.00±1.16	89.00±2.89	7.29

品种 Variety	胚根长Radicle length			胚芽长Blastocyst length
品种 Variety	CK(cm)	20%PEG(cm)	降幅(%)	CK(cm)	20%PEG(cm)	降幅(%)
1	7.17±0.27	7.28±1.89	-1.56	1.92±0.36	1.45±0.21	24.48
2	5.29±0.24	4.14±0.62	21.73	4.67±0.05	2.30±0.17^**	50.75
3	0.93±0.34	3.04±0.45^**	-226.81	1.21±0.08	1.62±0.08^*	-33.88
4	7.70±0.08	9.78±0.57^*	-27.02	1.67±0.17	1.59±0.11	4.79
5	6.29±0.67	4.16±0.13^*	33.87	2.57±0.92	1.88±0.20^*	26.87
6	3.59±0.70	3.36±0.44	6.41	1.97±0.24	1.64±0.21	16.75
7	6.36±0.21	4.15±0.82	34.74	4.40±0.21	2.33±0.30^**	47.04
8	5.72±0.81	2.51±0.52^**	56.12	3.13±0.09	1.36±0.18^**	56.55
9	5.23±0.16	2.39±0.04^**	54.30	3.81±0.13	1.81±0.26^**	52.49
10	6.88±0.18	3.10±0.56^**	54.93	3.53±0.02	1.51±0.08^**	57.22
11	6.17±0.25	2.75±0.18^**	55.43	2.61±0.18	1.02±0.08^**	60.92
12	6.44±0.06	2.58±0.18^**	59.94	2.67±0.11	1.11±0.12^**	58.42
13	4.23±0.19	3.81±0.53	9.92	3.81±0.51	2.05±0.17^*	46.20
14	7.48±0.20	4.10±0.21^**	45.19	3.32±0.20	1.37±0.97^**	58.71
15	5.60±0.38	3.05±0.48^**	45.53	2.45±0.88	1.76±0.07^**	28.19

Drought resistance identification and physiological changes of different millet varieties during germination

不同谷子品种萌发期抗旱鉴定及生理变化

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 28

Related Articles 15

Recommended Articles 0

Metrics

品种 Variety	胚芽鲜重 Fresh weight of embryo			胚芽干重 Embryo dry weight
品种 Variety	CK(g)	20%PEG(g)	降幅(%)	CK(g)	20%PEG(g)	降幅(%)
1	0.031±0.004	0.031±0.003	0.00	0.007±0.001	0.008±0.001	-14.29
2	0.053±0.001	0.040±0.003^*	24.53	0.012±0.000	0.010±0.001	16.67
3	0.017±0.002	0.045±0.003^**	-164.70	0.004±0.000	0.010±0.000^**	-150.00
4	0.041±0.009	0.041±0.004	0.00	0.009±0.001	0.009±0.011	-0.01
5	0.048±0.002	0.030±0.003^**	37.50	0.007±0.000	0.007±0.001	0.00
6	0.044±0.004	0.029±0.004	34.09	0.010±0.000	0.009±0.000	10.00
7	0.065±0.005	0.045±0.005^*	30.77	0.011±0.001	0.010±0.001	9.09
8	0.045±0.002	0.026±0.004^*	42.22	0.008±0.000	0.007±0.001	12.50
9	0.055±0.004	0.033±0.004^*	40.00	0.008±0.000	0.007±0.001	12.50
10	0.057±0.002	0.034±0.002^**	40.35	0.013±0.000	0.009±0.000^**	30.77
11	0.038±0.001	0.016±0.004^**	57.89	0.007±0.000	0.004±0.001^*	42.86
12	0.045±0.007	0.022±0.002^*	51.11	0.007±0.001	0.004±0.001	42.86
13	0.087±0.009	0.052±0.006^*	40.23	0.018±0.002	0.012±0.002	33.33
14	0.053±0.001	0.025±0.002^**	52.83	0.009±0.001	0.006±0.000^*	33.33
15	0.034±0.001	0.007±0.001^**	79.41	0.007±0.000	0.002±0.000^**	71.43

品种 Variety	隶属函数值Subordinate function values								综合 D值 Compreh ensive D value	位次 Seating arrange ment
品种 Variety	萌发抗旱指数 Drought resistance index	相对发芽率 Relative germination ratl	相对发芽势 Relative germination potential	相对胚根长 Relative radicle length	相对胚芽长 Relative blastocyst length	相对芽鲜重 Relative fresh weight of embryo	相对芽干重 Relative embryo dry weight	活力抗旱指数 Dynamic drought resistance index	综合 D值 Compreh ensive D value	位次 Seating arrange ment
1	0.517	0.172	0.864	1.000	0.687	0.999	0.307	0.547	0.657	4
2	1.000	0.997	1.000	0.655	0.282	0.696	0.243	0.727	0.679	2
3	0.472	0.727	0.456	0.389	0.517	0.894	1.000	0.901	0.685	1
4	0.500	0.968	0.642	0.590	1.000	1.000	0.320	0.188	0.677	3
5	0.859	0.757	0.917	0.448	0.649	0.536	0.248	0.554	0.614	5
6	0.498	1.000	0.288	0.917	0.807	0.584	0.275	0.412	0.591	6
7	0.528	0.114	0.620	0.433	0.337	0.620	0.259	0.824	0.476	8
8	0.544	0.572	0.793	0.070	0.194	0.477	0.218	1.000	0.478	7
9	0.593	0.568	0.928	0.100	0.253	0.497	0.236	0.315	0.435	9
10	0.661	0.525	0.539	0.089	0.181	0.492	0.161	0.359	0.377	10
11	0.712	0.554	0.874	0.080	0.126	0.263	0.105	0.277	0.358	12
12	0.620	0.934	0.524	0.000	0.163	0.342	0.131	0.165	0.348	13
13	0.000	0.689	0.091	0.857	0.350	0.492	0.148	0.283	0.358	11
14	0.287	0.601	0.401	0.256	0.159	0.334	0.147	0.286	0.301	14
15	0.137	0.000	0.000	0.251	0.000	0.000	0.000	0.000	0.041	15

[1]	LI Yongtai, GAO Axiang, LI Yanjun, ZHANG Xinyu. Effects of defoliants on the physiological characteristics of cotton varieties with different sensitivities [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2094-2102.
[2]	Arezigu Tuxun, JIA Kai, GAO Jie. Effects of different substrates and planting densities on onion bulblet yield [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1993-2003.
[3]	LIU Huijie, WANG Junhao, GONG Zhaolong, LIANG Yajun, WANG Junduo, LI Xueyuan, ZHENG Juyun, WANG Jichuan. Identification of salt tolerance of 197 upland cotton varieties at germination stage [J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1574-1581.
[4]	ZHAO Yun, FENG Guojun, Gulizhati Bazierbieke, HU Xiangwei, Subinuer Kadeer, LI Pengbing, SHAO Jiang, LIU Jie. Effects of potassium fertilizer dosage on growth and yield of drip irrigated millet in northern Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(6): 1378-1385.
[5]	HAO Xiyu, LIU Tingting, WANG Hui, LENG Jingwen, GONG Shihang, LIU Wei, LIANG Jie. Comprehensive evaluation of foxtail millet varieties based on entropy weight method and grey relational analysis [J]. Xinjiang Agricultural Sciences, 2024, 61(12): 2902-2912.
[6]	YU Xinyu, YU Jing, FENG Naihong, YUE Zhongxiao, HOU Donghui, YANG Chengyuan. Effects of winter sowing on the nutrition and flavor of Jinfen 107 millet [J]. Xinjiang Agricultural Sciences, 2024, 61(12): 2913-2920.
[7]	SHAO Jiang, ZHAO Yun, HU Xiangwei, LIU Jie, Nasirula Keremu, SHI Shubing, FENG Guojun. Effects of drought stress on foxtail millet yield and dry matter accumulation in different periods [J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2388-2395.
[8]	JU Le, QI Juncang, CHEN Peiyu, NIU Yinting, YIN Zhigang. Effects of drought stress on seed germination, seedling growth and physiological characteristics of barley [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1879-1886.
[9]	FENG Guojun, HU Xiangwei, ZHAO Yun, YU Ming, ZHANG Shugong, ZHOU Daoliang. Research progress, problems and development prospect of foxtail millet industry in Xinjiang [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 1887-1893.
[10]	QU Kejia, SHI Xiaolei, ZHANG Heng, WANG Xingzhou, GENG Hongwei, DING Sunlei, ZHANG Jinbo, YAN Yongliang. Evaluation of drought resistance of introduced spring wheat under PEG treatment [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1363-1371.
[11]	CHEN Liliang, LU Qianjun, MA Yuanyuan, LIU Ying, ZHAO Baolong, SUN Junli. Effects of NaCl stress on antioxidant enzyme content and malondialdehyde content in grape cultivars leaves [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 880-888.
[12]	SHAN Ying, LI Yue, XU Min, LIU Yanzhen, LI Ran, CHEN Jieyin, WANG Zisheng, ZHU He. Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress [J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2653-2660.
[13]	ZHAO Yun, FENG Guojun, HU Xiangwei, LI Cuimei, LI Pengbing, Akbota Muheyati, BAI Ling. Analysis of main agronomic characters and yield of different replanted millet varieties [J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2453-2460.
[14]	ZHENG Zipiao, XU Haijiang, CUI Jianping, LIN Tao, GUO Rensong, WANG Liang, ZHANG Dawei, WEI Xin, KONG fanyang. Effects of Water Stress on Growth and Development in Cotton （Gossypium hirsutum L.） [J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1821-1830.
[15]	LIU Zhigang, REN Hongsong, Huxidan Maimaiti, WANG Ruihua, LI Haifeng, HU Guozhi. Effects of Exogenous Calcium on Physiological Characteristics of Muskmelon Seedling Leaves during High Temperature Period [J]. Xinjiang Agricultural Sciences, 2022, 59(3): 588-596.