Effect of pomegranate peel flesh extract on body weight gain and plasma Immunity-related indexes in adult mice

doi:10.6048/j.issn.1001-4330.2023.08.030

Abstract

Abstract:

【Objective】 This experiment aims to investigate the effects of pomegranate peel flesh extract on body weight, plasma antioxidant and pro-inflammatory factor levels in mice in the hope of providing a theoretical basis for further research on the biological effects of pomegranate peel flesh extract.【Methods】 Thirty adult male mice with an average body weight of (37.31±5.57) g were randomly divided into 2 groups: the control group and the test group, with 5 replicates of 3 mice in each group, for a total of 15 mice.Mice in the test group were fed 1.5 g/(d-animal) of crude extract of pomegranate peel flesh [i.e., ellagic acid at 40.74 mg/(d-animal)] under the same basal diet, and mice in the control group were fed the corresponding quantity of sterile distilled water for 21 days.At the end of the experiment, mice were weighed on an empty stomach before morning feeding, and blood was collected from the mice with their tails severed.【Results】 The results showed that (1) the end weight, total weight gain and mean daily weight gain of mice in the test group were significantly increased by 8.21% (P<0.05), 47.56% (P<0.05) and 48.39% (P<0.05), respectively, compared with the control group; (2) the plasma levels of SOD, CAT, and T-AOC in mice in the test group were significantly increased by 24.44% (P< 0.05), 54.82% (P<0.05), and 27.69% (P<0.05), respectively, and the ability to inhibit hydroxyl radicals and anti-superoxide anion activity were highly significantly increased by 36.01% (P<0.01) and 43.21% (P<0.01), respectively, compared with the control group, which significantly reduced the concentration of MAD in the plasma of mice in the test group (P<0.05); (3) the plasma levels of TNF mice plasma TNF-α and IL-17 were significantly reduced by 17.16% (P<0.05) and 30.34% (P<0.05), respectively, compared with the control group.【Conclusion】 In conclusion, under the present experimental conditions, the administration of pomegranate peel flesh extract to mice significantly increases their body weight and improves their antioxidant capacity and anti-inflammatory ability.

Key words: pomegranate peel flesh extract; mice; body weight; antioxidant; inflammation

摘要：

【目的】研究石榴皮瓤提取物对小鼠体重、血浆抗氧化和促炎因子水平的影响。【方法】选择平均体重(37.31±5.57) g的成年小鼠30只,随机分为2组,每组15只,分别为对照组和试验组。在相同基础日粮条件下试验组小鼠灌喂1.5 g/(d·只)石榴皮瓤粗提物[即鞣花酸的灌喂量为40.74 mg/(d·只)],对照组小鼠灌喂相应体积的无菌蒸馏水,灌喂试验21 d。试验结束当天,小鼠晨饲前空腹称量体重,断尾采集小鼠血液。【结果】(1)试验组小鼠的末重、总增重和平均日增重分别比对照组显著提高了8.21%(P<0.05)、47.56%(P<0.05)和48.39%(P<0.05);(2)试验组小鼠血浆中SOD、CAT、和T-AOC水平分别比对照组显著提高了24.44%(P<0.05)、54.82%(P<0.05)和27.69%(P<0.05),抑制羟自由基能力和抗超氧阴离子活力分别比对照组极显著提高了36.01%(P<0.01)和43.21%(P<0.01),显著降低了试验组小鼠血浆中MAD的浓度(P<0.05);(3)试验组小鼠血浆中TNF-α和IL-17分别比对照组显著降低了17.16%(P<0.05)和30.34%(P<0.05)。【结论】对小鼠灌喂石榴皮瓤提取物可显著增加小鼠体重,提高小鼠抗氧化能力和抗炎症能力。

关键词: 石榴皮瓤提取物, 小鼠, 体重, 抗氧化, 炎症

CLC Number:

S852

LIU Jiajia, HAN Tao. Effect of pomegranate peel flesh extract on body weight gain and plasma Immunity-related indexes in adult mice[J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2074-2080.

刘佳佳, 韩涛. 石榴皮瓤提取物对成年小鼠体重增长及血浆免疫相关指标的影响[J]. 新疆农业科学, 2023, 60(8): 2074-2080.

Figures/Tables 5

References 37

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原料 Ingredients	比例 Proportion	营养水平 Nutrition level	含量 Content
小麦粉Wheat flour	35.00	干物质DM	93.48
玉米Corn	20.00	有机物OM	91.67
豆粕Bean pulp	23.00	粗蛋白质CP	15.56
鱼粉Fish Meal	8.00	中性洗涤纤维NDF	17.95
葵饼Sunflower cake	8.00	酸性洗涤纤维ADF	7.50
肉骨粉 Meat and bone powder	4.00	钙Ca	1.69
食盐NaCl	1.00	磷P	0.12
磷酸氢钙CaHPO₄	0.50
石粉Limestone	0.48
多种维生素预混料 Premix¹⁾	0.02
合计Total	100

原料 Ingredients	比例 Proportion	营养水平 Nutrition level	含量 Content
小麦粉Wheat flour	35.00	干物质DM	93.48
玉米Corn	20.00	有机物OM	91.67
豆粕Bean pulp	23.00	粗蛋白质CP	15.56
鱼粉Fish Meal	8.00	中性洗涤纤维NDF	17.95
葵饼Sunflower cake	8.00	酸性洗涤纤维ADF	7.50
肉骨粉 Meat and bone powder	4.00	钙Ca	1.69
食盐NaCl	1.00	磷P	0.12
磷酸氢钙CaHPO₄	0.50
石粉Limestone	0.48
多种维生素预混料 Premix¹⁾	0.02
合计Total	100

项目 Items	对照组 Control Group	试验组 Trial Group	P值 P-value
日均采食量 Average daily feed intake(g)	5.12±0.14	5.36±0.21	0.121
初始重 Original weight(g)	37.08±4.09	37.53±6.17	0.813
末重 Final weight(g)	43.63±4.87^b	47.21±3.95^a	0.041
总增重 Total weight(g)	6.56±3.07^b	9.68±4.54^a	0.046
日增重 Average daily gain (g/day)	0.31±0.15^b	0.46±0.22^a	0.046

项目 Items	对照组 Control Group	试验组 Trial Group	P值 P-value
日均采食量 Average daily feed intake(g)	5.12±0.14	5.36±0.21	0.121
初始重 Original weight(g)	37.08±4.09	37.53±6.17	0.813
末重 Final weight(g)	43.63±4.87^b	47.21±3.95^a	0.041
总增重 Total weight(g)	6.56±3.07^b	9.68±4.54^a	0.046
日增重 Average daily gain (g/day)	0.31±0.15^b	0.46±0.22^a	0.046

项目 Items	对照组 Control Group	试验组 Trial Group	P值 P-value
总蛋白 TP (g/L)	47.84±3.20	51.06±2.687	0.123
白蛋白 ALB (g/)L	23.81±0.89	25.57±1.75	0.123
球蛋白 GLB (g/L)	24.03±0.56	25.49±2.11	0.126
尿素氮 BUN (mmol/L)	4.42±1.34	2.94±0.72	0.089