Effects of supplemental feeding amino acids on growth and blood biochemical indices of lactating foals under grazing conditions

doi:10.6048/j.issn.1001-4330.2024.01.026

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (1): 241-252.DOI: 10.6048/j.issn.1001-4330.2024.01.026

• Agricultural Equipment Engineering and Mechanization·Animal Husbandry Veterinarian • Previous Articles Next Articles

Effects of supplemental feeding amino acids on growth and blood biochemical indices of lactating foals under grazing conditions

JING Hongxin¹(), LI Jiahao¹, GUO Qingyong², ZHONG Mingbin¹, ZAHNG Chengming¹, MA Zhuang¹, Abudoushalamu Aihemaiti¹, LI Xiaobin¹^,²()

1. Xinjiang Key Laboratory of Herbivore Nutrition for Meat & Milk Production,College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China
2. Postdoctoral Mobile Station of College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China

Received:2023-04-22 Online:2024-01-20 Published:2024-02-21
Correspondence author: LI Xiaobin(1988-),male,associate professor,master supervisor,research direction:nutrition and metabolism of herbivore,(E-mail) 172387243@qq.com
Supported by:
Postdoctoral Sustentation Fund of Xinjiang Agricultural University(2021);Project for Key Laboratory Construction of Xinjiang Uygur Autonomous Region(2022D04004);National Innovation Project for College Students of Xinjiang Agricultural University(2023)

放牧条件下泌乳母马补喂氨基酸对哺乳马驹生长发育及血液生化指标的影响

经宏鑫¹(), 李佳豪¹, 郭庆勇², 钟明彬¹, 张城铭¹, 马壮¹, 阿布都沙拉木·艾合麦提¹, 李晓斌¹^,²()

1.新疆农业大学动物科学学院/新疆肉乳用草食动物营养实验室,乌鲁木齐 830052
2.新疆农业大学动物医学学院/兽医学博士后流动站,乌鲁木齐 830052

通讯作者: 李晓斌(1988-),男,甘肃天水人,副教授,硕士生导师,研究方向为草食动物营养代谢,(E-mail)172387243@qq.com
作者简介:经宏鑫(2000-),男,吉林四平人,硕士研究生,研究方向为动物营养与饲料,(E-mail)309221066@qq.com
基金资助:
新疆农业大学博士后基金项目(2021);新疆维吾尔自治区重点实验室项目(2022D04004);国家级大学生创新项目(2023)

Abstract

Abstract:

【Objective】 The purpose of this experiment is to investigate the effects of amino acid supplementation on lactating mares under grazing conditions with a view of promoting the growth and development of lactating foals and blood biochemical indexes by improving milk yield and milk quality,and providing reference for the healthy growth of lactating foals under the condition of female milking. 【Methods】 Twenty-four Yili mares with similar foaling date(May) and 30 days of lactation were selected. They were 7-9 years old with 4-5 parities. The average daily 8 h milking volume from foaling to 30 days was (2.79±0.02) kg. The average weight of the 24 foals was (71.36±14.3) kg,8 females and 16 males. According to the sex of the foals,24 foals were randomly divided into 4 groups with 6 mares(2 females and 4 males) in each group,which were control group,trial group Ⅰ,trial group Ⅱ and trial group Ⅲ,respectively. The foal groups were corresponding to the mares. Under the same grazing conditions(grazing time,drinking time,milking time and grazing pasture were the same),the control group was not fed any amino acid supplement,trial group Ⅰ was fed lysine 40 g/(d· horse),+ threonine 20 g/(d· horse),trial group Ⅱ was fed lysine 60 g/(d· horse),+ threonine 40 g/(d· horse). Test group Ⅲ was supplemented with lysine 80 g/(d· horse) + threonine 60 g/(d· horse). The supplementary feeding experiment lasted for 120 days. 【Results】 (1) In terms of body weight and body size,compared with the control group,trial group Ⅰ and trial group Ⅱ could significantly increase the body weight at 60 days,body height at 60 days and 90 days,total increase,and body skew length at 30 days and 120 days of nursing foals increased by 8.37%,4.44%,4.27%,24.22%,5.31%,5.20% and 6.25%,4.05%,4.09%,22.14%,5.55%,6.22%(P<0.01),respectively. At the same time,experimental group Ⅰ could significantly increase the breast circumference and total breast circumference of lactating foals at 60,90 and 120 days by 6.34%,9.38%,9.52% and 33.83%,respectively(P<0.05). Experimental group Ⅲ could only significantly increase the body height at 60 days,chest circumference at 61-90 days and 91-120 days,which were increased by 3.53%(P<0.01),25.17%(P<0.05) and 21.26%(P<0.05),respectively. There were no significant differences in tube circumference related indexes between experimental groups and control group(P > 0.05). (2) In terms of blood biochemical indicators,The levels of Glu,ALT,AST and ALP in the blood of nursing foals in experimental groups were significantly increased by 13.08%,33.91%,26.93%,6.19%,17.08%,38.18%,26.69%,9.43% and 13.08%,respectively,compared with the control group 12.02%,38.14%,18.38%,6.35%(P<0.01),meanwhile,The contents of TP,UA,T-Bil,γ-GT and Ca²⁺ in blood of nursing foals in trial groups Ⅰ and Ⅱ were significantly increased by 6.19%,23.44%,29.89%,15.47%,15.02% and 15.02%,respectively 6.67%,31.18%,30.07%,20.02%,14.00%(P<0.01). 【Conclusion】 Supplementing lysine and threonine can significantly promote the growth of body weight and body size of lactating mares under grazing conditions,increase the contents of TP,ALB,GLB,Glu and Ca²⁺ in blood of lactating mares,increase the activity of ALT,AST and ALP,thus promoting the growth and development of foals. Supplementation of 40-60 g/(d·horse) lysine and 20-40 g/(d·horse) threonine to lactating mares can promote the growth and development of foals and body health.

Key words: in lactation nurse horse; lysine; threonine; lactation pony; body weight; blood biochemical indexes

摘要：

【目的】放牧条件下为泌乳母马补喂氨基酸,分析补喂对哺乳马驹生长发育及血液生化指标的影响,为哺乳马驹在母马挤乳条件下健康生长提供参考依据。【方法】选择产驹日期相近(5月),泌乳30 d的伊犁马母马24匹,年龄7~9岁,胎次4~5胎,产驹至30 d日均8 h挤奶量为(2.79±0.02) kg。24匹马驹平均体重为(71.36±14.3) kg,8母16公。结合马驹性别将24匹马驹随机分为4个组,每组6匹(2母,4公),分别为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组,马驹分组与母马相对应。在相同放牧条件下(放牧时间、饮水时间、挤奶时间和放牧草场完全相同),对照组不进行任何氨基酸补喂,试验Ⅰ组补喂赖氨酸40 g/(d·匹)+苏氨酸20 g/(d·匹),试验Ⅱ组补喂赖氨酸60 g/(d·匹)+苏氨酸40 g/(d·匹),试验Ⅲ组补喂赖氨酸80 g/(d·匹)+苏氨酸60 g/(d·匹)。进行为期为120 d的补饲试验。【结果】(1)试验Ⅰ组和试验Ⅱ组与对照组相比能够极显著提高哺乳马驹的60 d体重、60 d体高、90 d体高、总增高、30 d体斜长和120 d体斜长,分别提高了8.37%、4.44%、4.27%、24.22%、5.31%、5.20%和6.25%、4.05%、4.09%、22.14%、5.55%、6.22%(P<0.01)。试验Ⅰ组能够显著提高哺乳马驹60 d胸围、90 d胸围、120 d胸围和胸围总增长,分别提高了6.34%、9.38%、9.52%和33.83%(P<0.05);试验Ⅲ组仅能够显著提高哺乳马驹60 d的体高、61~90 d和91~120 d胸围增长,分别提高3.53%(P<0.01)、25.17%(P<0.05)和21.26%(P<0.05),另外,各试验组在管围相关指标与对照组均无显著差异(P>0.05)。(2)各试验组哺乳马驹血液中 Glu、ALT、AST 和 ALP 的含量与对照组相比分别极显著提高了 13.08%、 33.91%、26.93%、6.19%、17.08%、38.18%、26.69%、9.43%和 12.02%、38.14%、18.38%、6.35%(P<0.01),同时,试验Ⅰ组和试验Ⅱ组哺乳马驹血液中 TP、UA、T-Bil、γ-GT 和 Ca²⁺的含量分别极显著提高了 6.19%、23.44%、29.89%、15.47%、15.02% 和 6.67%、31.18%、30.07%、20.02%、14.00%(P<0.01)。【结论】在放牧条件下为泌乳母马补喂赖氨酸和苏氨酸,能够显著促进哺乳马驹体重、体尺的增长,同时提高马驹血液中TP、ALB、GLB、Glu和Ca²⁺的含量,增加ALT、AST和ALP的活力,进而促进马驹的生长发育,补喂泌乳母马40~60 g/(d·匹)赖氨酸和20~40 g/(d·匹)苏氨酸,能够促进马驹的生长发育和机体健康。

关键词: 泌乳母马, 赖氨酸, 苏氨酸, 哺乳马驹, 体重体尺, 血液生化指标

CLC Number:

S821

JING Hongxin, LI Jiahao, GUO Qingyong, ZHONG Mingbin, ZAHNG Chengming, MA Zhuang, Abudoushalamu Aihemaiti, LI Xiaobin. Effects of supplemental feeding amino acids on growth and blood biochemical indices of lactating foals under grazing conditions[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 241-252.

经宏鑫, 李佳豪, 郭庆勇, 钟明彬, 张城铭, 马壮, 阿布都沙拉木·艾合麦提, 李晓斌. 放牧条件下泌乳母马补喂氨基酸对哺乳马驹生长发育及血液生化指标的影响[J]. 新疆农业科学, 2024, 61(1): 241-252.

Figures/Tables 8

References 38

[1]	Graham-Thiers P M, Wilson J A, Haught J, et al. Relationships between dietary,plasma,and muscle amino acids in horses[J]. The Professional Animal Scientist, 2012, 28(3):351-357. DOI URL
[2]	Fišera M, Kráămar S, ustová K, et al. Effects of the lactation period,breed and feed on amino acids profile of mare's milk[J]. Potravinarstvo Slovak Journal of Food Sciences, 2020, 14:562-572. DOI URL
[3]	Liang X S, Yue Y X, Zhao Y L, et al. Effects of dietary concentrate to forage ratio on milk performance,milk amino acid composition and milk protein synthesis of lactating donkeys[J]. Animal Feed Science and Technology, 2022, 292:115444. DOI URL
[4]	孙雅璐. 蛋氨酸、赖氨酸补饲对奶山羊泌乳性能及氮利用效率的影响研究[D]. 杭州: 浙江大学, 2020.
	SUN Yalu. Effects of methionine and lysine supplementation on lactation performance and nitrogen use efficiency of dairy goats[D]. Hangzhou: Zhejiang University, 2020.
[5]	Graham P M, Ott E A, Brendemuhl J H, et al. The effect of supplemental lysine and threonine on growth and development of yearling horses[J]. Journal of Animal Science, 1994, 72(2):380-386. PMID
[6]	Committee on Nutrient Requiremrnts of Horses,National Research Council. Nutrient Requiements of Horses:Sixth Revised Edition[M]. National Academy of Sciences,1978.
[7]	Mok C H, Urschel K L. Amino acid requirements in horses[J]. Asian-Australasian Journal of Animal Sciences, 2020, 33(5):679-695.
[8]	Staniar W B. Growth of Thoroughbreds fed Different Levels of Protein and Supplemented with Lysine and Threonine[J]. Virginia Tech, 1998.
[9]	刘保仓. 过瘤胃赖氨酸在育肥牛羊精补料中的添加效果研究[D]. 石河子: 石河子大学, 2014.
	LIU Baocang. Study on the additive effect of rumen lysine in fine supplement of fattening cattle and sheep[D].. Shihezi: Shihezi University, 2014.
[10]	李仁德, 李光玉, 王凯英. 苏氨酸对动物的生物学作用研究进展[J]. 饲料工业, 2017, 38(8):36-39.
	LI Rendei, LI Guangyu, WANG Kaiying. Research progress of biological effects of threonine on animals[J]. Feed Industry, 2017, 38(8):36-39.
[11]	Glade M J. Dietary yeast culture supplementation of mares during late gestation and early lactation:Effects on milk production,milk composition,weight gain and linear growth of nursing foals[J]. Journal of Equine Veterinary Science, 1991, 11(2):89-95. DOI URL
[12]	Hintz H F, Schryver H F, Lowe J E. Comparison of a blend of milk products and linseed meal as protein supplements for young growing horses[J]. Journal of Animal Science, 1971, 33(6):1274-1277. PMID
[13]	阿依沙依拉. 补饲对哈萨克马和伊犁马哺乳马驹生长发育的影响[D]. 乌鲁木齐: 新疆农业大学, 2013.
	Ayishayila. Effects of supplementary feeding on growth and development of Kazak horse and Yili horse lactating foals[D]. Urumqi: Xinjiang Agricultural University, 2013.
[14]	Robbins K R. Threonine requirement of the broiler chick as affected by protein level and source[J]. Poultry Science, 1987, 66(9):1531-1534. PMID
[15]	日本中央赛马会竞走马综合研究所. 芒来.译. 轻型马饲养标准[M]. 北京: 农业大学出版社. 2007.
	Central Jockey Club Racewalking Institute of Japan. (Translated by Meng L.) Trans. Light Horse Breeding Standard[M]. Beijing: Agricultural University Press. 2007.
[16]	Niekerk F E, Niekerk C H. The effect of dietary protein on reproduction in the mare. Ⅱ. Growth of foals,body mass of mares and serum protein concentration of mares during the anovulatory,transitional and pregnant periods[J]. Journal of the South African Veterinary Association, 1997, 68(3):81-85. PMID
[17]	H.E.Hintz, 左绍群. 马的能量和蛋白质需要(续)[J]. 国外畜牧学(草食家畜), 1987,(1):43-44.
	H.E. Hentz, ZUO Shaoqun. Energy and protein requirements of equine[J]. Animal Science Abroad(Herbivorous Livestock), 1987,(1):43-44.
[18]	漆雯雯, 张浩, 杨魁, 等. 不同蛋白、能量水平对伊犁马泌乳后期生化指标及生殖激素的影响[J]. 家畜生态学报, 2015, 36(7):51-56.
	QI Wenwen, ZHANG Hao, YANG Kui, et al. Effects of Different Protein and Energy Levels on Biochemical Indexes and reproductive Hormones in late lactation of Yili horse[J]. Chinese Journal of Animal Ecology, 2015, 36(7):51-56.
[19]	方美烟, 王贤东, 于全平, 等. 不同消化能、粗蛋白质水平饲粮对泌乳前期伊犁马营养物质消化代谢、血液生理生化指标和激素的影响[J]. 草业学报, 2020, 29(10):129-138. DOI
	FANG Meiyan, WANG Xiandong, YU Quanping, et al. Effects of Different Digestible Energy and Crude Protein Levels on Nutrient Digestion and Metabolism,Blood Physiological and Biochemical Indexes and Hormones of Yili Horses during Early lactation[J]. Acta Prataculturae Sinica, 2020, 29(10):129-138. DOI
[20]	何宝祥, 马径军, 张宇, 等. 围产期奶牛肾功能的动态变化[J]. 中国兽医杂志, 2009, 45(8):45-46.
	HE Baoxiang, MA Jingjun, ZHANG Yu, et al. Dynamic changes of renal function in cows during perinatal period[J]. Chinese Journal of Veterinary Medicine, 2009, 45(8):45-46.
[21]	王光柱. 赖氨酸在单胃动物营养方面的研究进展[J]. 畜牧兽医科学(电子版), 2021,(12):191-192.
	WANG Guangzhu. Research progress of lysine in nutrition of single stomach animals[J]. Animal Husbandry and Veterinary Science(Electronic Ed.), 2021,(12):191-192.
[22]	Stull C L, Rodiek A V. Responses of blood glucose,insulin and cortisol concentrations to common equine diets[J]. Journal of Nutrition, 1988, 118(2):206-213. PMID
[23]	姚喜喜, 刘皓栋. 日粮蛋白水平对小尾寒羊羔羊生长性能、营养物质表观消化率、氮排放和血清生化指标的影响[J]. 草业科学, 2022, 39(2):362-370.
	YAO Xixi, LIU Haodong. Effects of Dietary Protein Levels on Growth Performance,Nutrient Apparent Digestibility,Nitrogen Emission and Serum Biochemical Indices of Small Tail Cold Lambs[J]. Pratacultural Science, 2022, 39(2):362-370.
[24]	张思聪, 张艳蕾, 李福昌. 苏氨酸的代谢及其营养生理作用[J]. 饲料研究, 2012,(7):14-16.
	ZHANG Sicong, ZHANG Yanlei, LI Fuchang. Metabolism and Nutritional physiological effects of threonine[J]. Feed Research, 2012,(7):14-16.
[25]	Westermeier C. Feed intake and body weights of suckling sows and piglets in depend-ence of dietary threonine supple mentation. Contribution about the threonine requirement of suckling pigs[J]. Journal of Animal Physiology and Animal Nutrition, 1998, 79(1):33-45. DOI URL
[26]	Ma P, Wu Y, Zeng Q, et al. Oxidative damage induced by chlorpyrifos in the hepatic and renal tissue of Kunming mice and the antioxidant role of vitamin E[J]. Food and Chemical Toxicology, 2013, 58:177-183. DOI PMID
[27]	Fraser C.M.. 韩谦. 默克兽医手册[M]. 北京: 中国农业大学出版社,1997.
	Fraser C.M.,Ed. Han Qian. Merck Veterinary Manual[M].. Beijing: China Agricultural University Press,1997.
[28]	高士争, 葛长荣, 韩剑众. 中草药添加剂对生长肥育猪血液生理生化指标的影响[J]. 云南农业大学学报, 2002,(2):164-169.
	GAO Shizheng, GE Changrong, HAN Jianzhong. Effects of Chinese Herbal Medicine Additives on Blood Physiological and Biochemical Indexes of Growing-finishing Pigs[J]. Journal of Yunnan Agricultural University, 2002, 17(2):164-169.
[29]	朱明霞, 刘文强, 侯国生. 鲁西地方黑猪血液生化指标与肉量和肉质的相关性研究[J]. 畜牧与兽医, 2013, 45(8):62-66.
	ZHU Mingxia, LIU Wenqiang, HOU Guosheng. Study on the correlation between blood biochemical indexes and meat quantity and meat quality of Luxi local black pigs[J]. Animal Husbandry and Veterinary Medicine, 2013, 45(8):62-66.
[30]	刘玉兰, 黄晶晶, 范伟, 等. L- 精氨酸对脂多糖刺激断奶仔猪生产性能、血液生化指标和内脏器官重量的影响[J]. 动物营养学报, 2008, 20(2):140-145.
	LIU Yulan, HUANG Jingjing, FAN Wei, et al. Effects of L-arginine on Performance,Blood Biochemical Indices and Internal Organ Weight of Weaned Piglets stimulated by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2008, 20(2):140-145.
[31]	Castejbn F, Trigo P, Mufioz A,et a1. Uric acid responses to endurance racing and relationships with performance,plasma biochemistry and metabolic alterations[J]. Equine Veterinary Journal, 2006, 38(36):70-73.
[32]	喻梅辉, 关小兵, 胡冰, 等. 家畜血清中乳酸脱氢酶(LDH)同功酶的分离与测定[J]. 中国兽医杂志, 1983, 19(12):7-9.
	YU Meihui, GUAN Xiaobing, HU Bing, et al. Isolation and determination of lactate dehydrogenase(LDH) isozyme in livestock serum[J]. Chinese Journal of Veterinary Medicine, 1983, 19(12):7-9.
[33]	葛可右. 中国营养科学全书[M]. 北京: 人民卫生出版社, 2004.
	GE Keyou. Chinese Nutritional Sciences[M].. Beijing: People’s Medical Publishing House, 2004.
[34]	褚洪忠. 不同饲养管理条件对杂交伊犁马驹生长发育影响的研究[D]. 乌鲁木齐: 新疆农业大学, 2012.
	CHU Hongzhong. Study on the effects of different feeding management conditions on the growth and development of hybrid Yili foals[D].. Urumqi: Xinjiang Agricultural University, 2012.
[35]	田颖, 时明慧. 赖氨酸生理功能的研究进展[J]. 美食研究, 2014, 31(3):60-64.
	TIAN Ying, SHI Minghui. Research progress in the physiological function of lysine[J]. Journal of Researches on Dietetic Science and Culture, 2014, 31(3):60-64.
[36]	杨凤. 动物营养学[M]. 北京: 中国农业出版社.1993.
	YANG Feng. Animal Nutrition[M].. Beijing: China Agriculture Press,1993.
[37]	Crowe A, Morgan E H. Iron and copper interact during their uptake and deposition in the brain and other organs of developing rats exposed to dietary excess of the two metals[J]. The Journal of Nutrition, 1996, 126(1):183-194.
[38]	周桂莲, 韩友文. 影响铁吸收利用因素研究进展[J]. 动物营养学报, 2001, 13(1):6-13.
	ZHOU Guilian, HAN Youwen. Research progress of Factors affecting Iron absorption and utilization[J]. Chinese Journal of Animal Nutrition, 2001, 13(1):6-13.

试验天数 Test days(d)	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0	3.34±0.44	3.35±1.22	3.36±0.34	3.36±0.91
0~120	3.33±0.11^d	4.03±0.52^bB	4.21±0.45^aA	3.88±0.32^cB

试验天数 Test days(d)	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0	3.34±0.44	3.35±1.22	3.36±0.34	3.36±0.91
0~120	3.33±0.11^d	4.03±0.52^bB	4.21±0.45^aA	3.88±0.32^cB

项目 Iems	含量 Content (%)	营养水平 Nutrient levels (2)	含量 Content (%)
大麦 Barley	55.54	干物质DM	89.25
玉米 Corn	36.00	粗蛋白质CP	14.56
豆粕 Soybean meal	6.00	粗脂肪EE	4.08
磷酸氢钙 Calcium hydrogen carbonate	1.00	粗灰分Ash	2.82
预混料 Pre mixed material(1)	1.16	中性洗涤纤维NDF	13.36
		酸性洗涤纤维ADF	5.18
		钙Ca	0.31
		磷P	0.46
		消化能DE(MJ/kg)(2)	16.62
合计Total	100.00

项目 Iems	含量 Content (%)	营养水平 Nutrient levels (2)	含量 Content (%)
大麦 Barley	55.54	干物质DM	89.25
玉米 Corn	36.00	粗蛋白质CP	14.56
豆粕 Soybean meal	6.00	粗脂肪EE	4.08
磷酸氢钙 Calcium hydrogen carbonate	1.00	粗灰分Ash	2.82
预混料 Pre mixed material(1)	1.16	中性洗涤纤维NDF	13.36
		酸性洗涤纤维ADF	5.18
		钙Ca	0.31
		磷P	0.46
		消化能DE(MJ/kg)(2)	16.62
合计Total	100.00

试验期 Trial period	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0 d(kg)	70.40±20.16	71.80±14.75	71.50±15.20	72.00±7.55
30 d(kg)	80.75±13.20	84.70±18.66	85.50±22.34	84.40±19.70
60 d(kg)	99.38±13.76^Cc	107.70±16.02^Aa	106.00±21.38^ABa	102.60±23.59^BCb
90 d(kg)	116.63±12.15^b	128.64±6.42^a	125.40±3.71^ab	122.71±4.80^ab
120 d(kg)	133.88±10.77^b	144.91±3.58^a	139.41±5.34^ab	138.43±3.33^ab
0~30 d增重 0-30 days to gain weight(kg)	17.25±2.25^b	22.77±3.22^a	21.00±3.85^ab	18.38±2.39^ab
31~60 d增重 31-60 days to gain weight(kg)	18.63±0.85^Bb	23.40±1.92^Aa	19.30±2.59^Bb	18.20±2.02^Bb
61~90 d增重 61-90 days to gain weight(kg)	17.25±2.25^b	20.94±3.18^a	19.40±1.83^ab	20.11±1.67^ab
91~120 d增重 91-120 days to gain weight(kg)	15.75±2.47	17.93±3.53	16.21±2.29	16.68±1.21
总增重Total weight gain(kg)	70.38±6.42^b	78.97±2.52^a	74.64±4.30^ab	73.02±2.32^ab
平均日增重Average daily gain(kg)	0.57±0.06^Bb	0.66±0.01^Aa	0.63±0.01^ABa	0.62±0.02^ABab

Effects of supplemental feeding amino acids on growth and blood biochemical indices of lactating foals under grazing conditions

放牧条件下泌乳母马补喂氨基酸对哺乳马驹生长发育及血液生化指标的影响

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试验期 Trial period	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0 d(cm)	101.20±8.87	101.63±2.06	100.50±3.11	100.25±1.71
30 d(cm)	103.63±6.49	106.40±3.51	105.75±1.26	105.00±4.24
60 d(cm)	106.50±3.46^Bb	111.45±1.23^Aa	111.00±1.41^Aa	110.40±1.14^Aa
90 d(cm)	111.62±2.55^Bb	116.60±1.08^Aa	116.38±2.29^Aa	114.10±1.34^ABab
120 d(cm)	117.12±0.61^b	122.98±4.73^a	121.28±2.85^ab	119.09±3.28^ab
0~30 d增高0-30 d increase(cm)	4.88±2.06	4.88±4.25	5.25±3.77	5.67±2.31
31~60 d增高31-60 dincrease(cm)	5.00±1.00	5.31±1.11	5.25±0.96	5.50±0.58
61~90 d增高61-90 d dincrease(cm)	4.28±0.66	5.38±0.63	5.38±1.11	4.17±0.76
91~120 d增高91-120 d dincrease(cm)	4.50±1.41	5.10±0.79	4.90±0.91	4.62±0.42
总增高Total increase in height(cm)	16.18±2.21^Bb	21.35±0.61^Aa	20.78±2.56^Aab	18.84±0.30^ABab

试验期 Trial period	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0 d (cm)	83.50±7.72	83.75±4.79	83.00±7.83	83.33±4.73
30 d (cm)	90.67±0.58^Bc	95.75±1.50^Aa	96.00±0.82^Aa	93.20±2.28^ABb
60 d (cm)	98.90±0.70	102.50±1.29	101.25±4.86	99.25±0.96
90 d (cm)	104.77±1.38	107.20±1.64	106.80±1.64	106.00±1.80
120 d (cm)	108.63±0.32^Bb	114.59±1.71^Aa	115.88±1.31^Aa	108.67±1.61^Bb
0~30 d增长0-30 d growth(cm)	8.80±0.84^b	10.75±0.96^a	11.00±1.00^a	9.75±1.71^ab
31~60 d增长31-60 d growth(cm)	7.00±0.85	7.25±0.50	7.25±1.89	7.50±0.71
61~90 d增长61-90 d growth(cm)	5.70±0.14	6.80±1.48	5.00±1.41	5.75±2.33
91~120 d增长91-120 d growth(cm)	3.87±0.31^b	5.84±0.59^a	5.00±1.00^ab	4.00±1.00^b
体斜长总增长Total additive oblique length(cm)	24.97±4.43^Bb	30.84±1.01^ABa	32.88±3.12^Aa	25.33±1.26^Bb

试验期 Trial period	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0 d (cm)	95.75±10.59	95.25±5.25	94.25±8.77	96.00±4.24
30 d (cm)	98.00±8.28	101.00±8.63	101.60±9.29	100.60±9.46
60 d (cm)	104.52±8.61^b	111.60±1.67^a	108.00±2.89^ab	107.00±2.35^ab
90 d (cm)	108.92±9.01^b	120.20±5.64^ab	112.20±6.72^ab	112.70±6.25^ab
120 d(cm)	113.32±9.52^b	125.25±7.24^a	117.40±4.74^ab	118.80±7.16^ab
0~30 d增长0-30 d growth(cm)	5.50±0.58	7.50±4.04	7.50±2.08	7.33±5.77
31~60 d增长31-60 d growth(cm)	7.50±1.54	9.25±4.19	7.25±3.57	8.10±1.95
61~90 d增长61-90 d growth(cm)	4.40±1.13^Bb	6.50±0.50^Aa	4.63±1.25^Bb	5.88±0.47^ABa
91~120 d增长91-120 d growth(cm)	4.40±1.13^Bc	7.60±1.14^Aa	5.75±0.65^ABbc	6.10±1.08^ABb
胸围总增长Total increase in chest size(cm)	19.85±8.16^b	30.00±3.81^a	24.88±5.86^ab	23.38±4.27^ab

试验期 Trial period	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ
0 d (cm)	12.80±1.30	12.81±0.24	12.75±0.96	12.50±0.58
30 d (cm)	13.17±0.91	13.75±0.29	13.58±0.43	13.20±0.47
60 d (cm)	14.47±0.92	15.10±0.73	14.55±0.31	14.50±0.41
90 d (cm)	15.73±0.57	16.05±0.95	15.80±0.53	15.78±0.26
120 d (cm)	16.90±0.52	17.18±1.10	17.00±0.83	16.98±0.21
0~30 d增长0-30 d growth(cm)	1.10±0.14	1.00±0.00	1.10±0.36	1.10±0.61
31~60 d增长31-60 d growth(cm)	1.08±0.40	1.28±0.75	1.18±0.76	1.14±0.44
61~90 d增长61-90 d growth(cm)	1.08±0.13	1.13±0.23	1.18±0.46	1.20±0.26
91~120 d增长91-120 d growth(cm)	1.05±0.10	1.13±0.39	1.06±0.52	1.06±0.36
总增管围Total additional pipe circumference	3.90±0.61	4.36±1.31	4.25±1.10	4.05±1.42

项目 Items	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ	P值P-value
项目 Items	对照组 Control group	试验Ⅰ组 Test group Ⅰ	试验Ⅱ组 Test group Ⅱ	试验Ⅲ组 Test group Ⅲ	T	D	T×D
总蛋白TP(g/L)	54.57±6.71^Bb	58.17±6.70^Aa	58.47±5.67^Aa	55.89±6.80^ABab	0.006	0.000	0.003
白蛋白ALB(g/L)	26.83±5.21	28.34±3.55	28.36±5.16	27.81±5.12	0.456	0.000	0.195
球蛋白GLB(g/L)	27.74±7.77	29.83±4.92	30.11±4.95	28.08±6.73	0.227	0.025	0.000
尿酸UA(μmol/L)	15.58±7.62^Bb	20.35±6.13^Aa	22.64±8.86^Aa	21.27±6.94^Aa	0.001	0.000	0.040
葡萄糖Glu(mmol/L)	5.05±1.03^Bb	5.81±0.88^Aa	6.09±0.89^Aa	5.74±0.96^Aa	0.000	0.000	0.231
甘油三酯TG(mmol/L)	0.26±0.11^ab	0.24±0.07^b	0.23±0.08^b	0.30±0.15^a	0.029	0.000	0.047
总胆红素T-Bil(μmol/L)	11.35±4.49^Bb	16.19±6.94^Aa	16.23±5.84^Aa	13.10±4.48^ABb	0.000	0.024	0.028
谷丙转氨酶ALT(U/L)	10.72±3.84^Bb	16.22±10.24^Aa	17.34±5.21^Aa	17.33±6.76^Aa	0.000	0.000	0.054
谷草转氨酶AST(U/L)	255.76±37.93^Cc	350.00±57.27^Aa	348.87±83.85^Aa	313.36±67.69^Bb	0.000	0.000	0.004
碱性磷酸酶ALP(U/L)	570.53±28.70^Bc	608.16±44.07^Ab	629.91±62.23^Aa	609.19±38.38^Ab	0.000	0.000	0.033
γ-谷氨酰转移酶 γ-GT(U/L)	19.18±4.07^Cc	22.69±3.78^ABab	23.98±4.03^Aa	20.85±5.26^BCbc	0.000	0.001	0.044
肌酸激酶CK(U/L)	123.42±21.90^Bbc	115.08±26.16^Bc	164.84±23.59^Aa	128.74±22.79^Bab	0.000	0.489	0.680
乳酸脱氢酶LDH(U/L)	416.93±43.14	406.54±51.22	407.34±37.84	423.49±47.82	0.368	0.297	0.042
钙Ca²⁺(mmol/L)	2.15±0.54^Bb	2.53±0.34^Aa	2.50±0.42^Aa	2.42±0.42^ABa	0.001	0.000	0.431
无机磷P(mmol/L)	2.27±0.55	2.53±0.50	2.45±0.49	2.54±0.63	0.126	0.007	0.012
镁Mg²⁺(mmol/L)	1.43±0.63^Aa	1.20±0.35^Bb	1.02±0.25^Bb	1.12±0.40^Bb	0.000	0.000	0.000
铁Fe²⁺(μmol/L)	44.11±10.38^Aa	49.06±11.46^Aa	49.14±10.66^Aa	36.90±10.52^Bb	0.0000	0.011	0.290

[1]	JING Hongxin, LI Minghao, WANG Jianwen, Abuduwaresi Abulikemu, LI Xiaobin. Effects of supplementing lysine and threonine on hormones in blood and milk of lactating mares [J]. Xinjiang Agricultural Sciences, 2024, 61(12): 3113-3120.
[2]	LIU Jiajia, HAN Tao. Effect of pomegranate peel flesh extract on body weight gain and plasma Immunity-related indexes in adult mice [J]. Xinjiang Agricultural Sciences, 2023, 60(8): 2074-2080.
[3]	LI Chao, LI Xiaobin, DENG Haifeng, HUANG Xinxin, YANG Fan, LI Hai, CHEN Kaixu. Study on the Changes of Body Weight and Body Size Increase of 0-12 Months Old Thoroughbred Horse Foal [J]. Xinjiang Agricultural Sciences, 2022, 59(6): 1545-1552.
[4]	CHEN Sheng-ao;YAO Na;SONG Yong;WANG Shuai;XIE Cong-xin. Effects of Morphological Traits on Body Mass of Triplophysa (Hedinichthys) yarkandensis (Day) [J]. , 2017, 54(3): 546-556.
[5]	. Study on Growth Curve Fitting of Xinjiang Brown Cattle and Their Hybrids [J]. , 2016, 53(3): 569-574.
[6]	. The Effect of Fartlek Endurance Training on Blood Biochemical Index of Young Yili Horse [J]. , 2016, 53(2): 367-375.
[7]	ZHAO Fang;Tunisa Maitisaiyidi;YANG Ju-qing;WU Ting-ting;LIU Zhen;YANG Kai-lun. Effects of Four Kinds of Oral Preparation on Concentration of Immunoglobulin in Plasma and Body Weight Gain of Newborn Lambs [J]. , 2015, 52(1): 180-184.
[8]	Julaiti Abuduwaili;ZHANG Hao;REN Wan-ping;L(U) Qiang;YU Xiong. Effects of Different Feeding Conditions on the Yong Xinjiang Brown Cattle's Weight Changes and Serum Hormone Level [J]. , 2014, 51(12): 2321-2327.
[9]	. Change, Correlation and Regression Analysis of Body Weight and Body Size Index in Kyrgyz Mare at Different Ages [J]. , 2014, 51(10): 1942-1947.
[10]	Wubuli Sadike;Yiming Sulaiman;Maimaiti Reheman;Abulizi Wusiman. Comparison of Body Size Indexes of New Hybrid F1 and F2 Generation of Bogeda Cashmere Goat and Luntai Native Cashmere Goats [J]. , 2013, 50(5): 963-966.