An Anatomical Study on Flower Bud Differentiation of Miguang and Baoguang

doi:10.6048/j.issn.1001-4330.2019.11.007

Abstract

Abstract: 【Objective】 The study aims to observe the initiation time of flower bud differentiation and the speed of differentiation in different grape cultivars in the hope of providing theoretical foundation for alternate year bearing. 【Method】 The paraffin section method was used to observe the bud structure in the process of flower (cultivars Miguang, Baoguang and Summer Black).【 Result 】 The results showed that the whole process of the morphological differentiation of flower bud could be divided into the undifferentiating stage, the early differentiation of inflorescence stage, the inflorescence anlagen appearance stage, the formation of the main axis of inflorescence primordium stage, and the second branching of inflorescence primordium stage. The peak period of flower bud differentiation of Summer Black , Miguang and Baoguang occurred between the early June and the early August(about 60 days), between the early June and the mid to late June (about 40 days) and between the late May and the mid to late June(about 50 days) respectively.【Conclusion 】 In general, the morphological characteristics were basically the same in each stage in the three cultivars. The process of flower bud differentiation was slower, the duration of each stage was relatively longer and the stages were more overlapped with each other of Summer Black than Miguang and Baoguang.

Key words: grape; flower bud; morphological differentiation

摘要： 【目的】研究葡萄花芽形态分化时间和各时期的特征,为解决葡萄大小年结果现象提供理论依据。【方法】以葡萄新品种蜜光和宝光为试材,夏黑为对照,采用石蜡切片法对花芽分化过程的花芽分化形态进行观察和对比分析。【结果】蜜光宝光和夏黑花芽分化均可分为未分化期、花序开始分化前期、花序原始体发现期、花序主轴期和花序第二穗轴期5个时期; 6月上旬到8月上旬为夏黑花芽的分化盛期(约60 d);6月上旬到7月中下旬和5月下旬到7月中下旬为蜜光( 约40 d)和宝光花芽的分化盛期(约50 d)。【结论】蜜光、宝光和夏黑花芽分化进程各个时期的形态特征基本相同。夏黑花芽分化进程较缓慢,各阶段持续时间相对较长且各阶段互相重叠;蜜光和宝光花芽分化相对集中,分化进程相对较快。

关键词: 葡萄, 花芽, 形态分化

CLC Number:

S663.1

JIA Nan, YUAN Jun-wei, HAN Bin, LI Min-min, LIU Chang-jiang, YIN Yong-gang, SUN yan, GUO Zi-juan ZHAO Sheng-jian. An Anatomical Study on Flower Bud Differentiation of Miguang and Baoguang[J]. Xinjiang Agricultural Sciences, 2019, 56(11): 2015-2022.

贾楠, 袁军伟, 韩斌, 李敏敏, 刘长江, 尹勇刚, 孙艳, 郭紫娟, 赵胜建. 蜜光和宝光葡萄品种花芽分化时间和各时期特征[J]. 新疆农业科学, 2019, 56(11): 2015-2022.

References

[1] 蔡文博, 段虹, 王军,等.4个鲜食葡萄品种组培快繁体系的建立[J]. 核农学报, 2019, 33(2): 248-254.
CAI Wen-bo, DUAN Hong, WANG Jun, et al. Establishment of in vitro rapid micropropagation of four table grape cultivars [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(2): 248-254.
[2] 杨亚蒙, 姜建福, 樊秀彩,等.2000年以来我国葡萄国外引种概况[J].中外葡萄与葡萄酒, 2018, (2): 13.
YANG Ya-meng, JIANG Jian-fu, FAN Xiu-cai, et al. Overview for introduction of grape varieties from overseas since 2000[J]. Sino-Overseas Grapevine ＆ Wine, 2018, (2): 13.
[3] 刘凤之.中国葡萄栽培现状与发展趋势[J]. 落叶果树, 2017, 49(1): 1-4.
LIU Feng-zhi. Current situation and development trend of viticulture in China [J]. Deciduous Fruits, 2017, 49(1): 1-4.
[4] Yamane, H., Ooka, T., Jotatsu, H., Sasaki, R., & Tao, R. Expression analysis of PpDAM5 and PpDAM6 during flower bud development in peach (Prunus persica). Scientia horticulturae, 2011,129(4):844-848.
[5] Sqnsteby, A., & Heide, O. M. Variation in seasonal timing of flower bud initiation in black currant (Ribes nigrum L.) cultivars of contrasting geographic origin. The Journal of Horticultural Science and Biotechnology, 2013,88(4): 403-408.
[6] Baldi, E., Colucci, E., Gioacchini, P., Valentini, G., Allegro, G., Pastore, C., & Toselli, M. Effect of post-bloom foliar nitrogen application on vines under two level of soil fertilization in increasing bud fertility of 'Trebbiano Romagnolo'(Vitis vinifera L.) vine. Scientia horticulturae, 2017,218: 117-124.
[7] 程和禾, 陈龙, 李玉生,等. 温室甜樱桃花芽形态分化观察[J].果树学报, 2018, 35(11) : 1393-1398.
CHENG He-he, CHEN Long, LI Yu-sheng, et al.Observation on the morphological differentiation of flower buds of sweet cherries in a greenhouse [J]. Journal of Fruit Science, 2018, 35(11) : 1393-1398.
[8] 魏雅君, 廖康, 李雯雯,等. 杏李花芽分化的组织解剖学研究[J].果树学报, 2017, 34(7) : 843-850.
WEI Ya-jun, LIAO Kang, LI Wen-wen, et al. An anatomical study on flower bud differentiation in Prunus salicina × armeniaca [J]. Journal of Fruit Science, 2017, 34(7) : 843-850.
[9] 艾云苾, 刘爱青, 韩婧, 等. 冬季土壤自然封冻始期 4 个品种芍药的花芽发育状态[J]. 西北林学院学报, 2016, 31(3): 119-123.
AI Yun-bi, LIU Ai-qing, HAN Jing, et al. Floral bud differentiation stage of 4 peony cultivars in the initial period of soil naturally frozen in winter [J]. Journal of Northwest Forestry University, 2016, 31(3): 119-123.
[10] 何崇单, 蔡雨蒙, 李萌,等. 花芽 3 个分化时期转录组测序的相关基因筛选与表达分析. [J]. 园艺学报, 2018, 45 (8): 1479-1490.
HE Chong-dan, CAI Yu-meng, LI Meng, et al. Screening and expression analysis of related genes based on transcriptome sequencing of ginkgo flower buds at three differentiation stages [J]. Acta Horticulturae Sinica, 2018, 45 (8): 1479-1490.
[11] Sreekantan, L., Mathiason, K., Grimplet, J., Schlauch, K., Dickerson, J. A., & Fennell, A. Y. Differential floral development and gene expression in grapevines during long and short photoperiods suggests a role for floral genes in dormancy transitioning. Plant molecular biology, 2010,73(1-2):191-205.
[12] Nair, N. G., & Allen, R. N. Infection of grape flowers and berries by Botrytis cinerea as a function of time and temperature. Mycological Research, 1993,97(8): 1012-1014.
[13] Smart, R. E., Turkington, C. R., & Evans, J. C. Grapevine response to furrow and trickle irrigation. American Journal of Enology and Viticulture, 1974,25(2): 62-66.
[14] Vaillant-Gaveau, N., Maillard, P., Wojnarowiez, G., Gross, P., Clément, C., & Fontaine, F. Inflorescence of grapevine (Vitis vinifera L.): a high ability to distribute its own assimilates. Journal of experimental botany, 2011,62(12):4,183-4,190.
[15] 司守霞, 牛娟, 陈利娜, 等. 不同药剂对石榴可育花比率及产量和品质的影响[J]. 西北林学院学报, 2017, 32(4): 111-116.
SI Shou-xia, NIU Juan, CHEN Li-na, et al.Effect of different reagents on the proportion of fertile flower and fruit yield and quality in of pomegranate [J]. Journal of Northwest Forestry University, 2017, 32(4): 111-116.
[16] 黄璐. 巨峰葡萄不同芽节位夏季花芽分化规律及相关影响因子的研究 [D] . 南宁:广西大学硕士论文, 2017, 32(4): 111-116.
HUANG Lu. Study on the summer differentiation of buds in different nodes and its influence factors in Kyoho grape [D]. Master Dissertation. Guangxi University, Nanning.(in Chinese)
[17] 路瑶, 段慧, 刘昆玉,等. 红地球葡萄花芽分化的观察[J]. 湖南农业科学, 2017,(9): 77-79.
LU Yao, DUAN Hui, LIU Kun-yu, et al. Observation of Flower Bud Differentiation of Red Globe Grape[J].Hunan Agricultural Sciences, 2017,(9): 77-79.
[18] 赵君全, 王海波, 王孝娣,等. 设施栽培条件下 '夏黑'葡萄花芽分化规律及环境影响因子研究. [J].果树学报, 2014, 31(5): 842-847.
ZHAO Jun-quan, WANG Hai-bo, WANG Xiao-di, et al. Flower bud differentiation of'Summer Black'grape in greenhouse and related environmental influence factors[J]. Journal of Fruit Science, 2014, 31(5): 842-847.
[19] 李正理.植物制片技术[M]. 北京:科学出版社, 1978.
LI Zheng-li.The technique of making slices of plants [M]. Beijing: Science Press. 1978.
[20] Srinivasan, C., & Mullins, M. G. Physiology of flowering in the grapevine-a review. American Journal of Enology and Viticulture, 1981,32(1): 47-63.
[21] 杨秀平, 岁立云, 王淑惠. 山桐子花芽分化及雌雄配子体发育[J]. 西北林学院学报, 2016, 31(6): 126-132.
YANG Xiu-ping, SUI Li-yun, WANG Shu-hui. Flower Bud Differentiation and Development of Male and Female Gametophytes in Idesia polycarpa [J]. Journal of Northwest Forestry University, 2016, 31(6): 126-132.
[22] 袁志友, 李宪利,孙庆华,等. 巨峰葡萄花芽分化的研究[J]. 西北植物学报, 2003, 23 (3):389-394.
YUAN Zhi-you, LI Xia-li, SUN Qing-hua, et al. Studies on flower bud differentiation in'Kyoho' grapevine [J]. Acta Botanica Boreali-occidentalia Sinica , 2003, 23 (3):389-394.
[23] 王海波, 王孝娣, 赵君全,等. 设施促早栽培下耐弱光能力不同的葡萄品种冬芽的花芽分化[J].园艺学报, 2016, 43(4): 633-642.
WANG Hai-bo, WANG Xiao-di, ZHAO Jun-quan, et al. Studies on the flower bud differentiation of grape cultivars with different tolerant ability of low light in greenhouse [J]. Acta Horticulturae Sinica, 2016, 43(4): 633-642.
[24] 刘丹, 孙欣, 慕茜,等. 葡萄花芽发育相关基因在不同节位芽中的表达分析[J]. 中国农业科学, 2015, 48(10): 2 007-2 016.
LIU Dan, SUN Xin, MU Qian, et al. Analysis of expression levels of floral genes in the buds on different branch nodes of grapevine [J]. Journal of Experimental Botany, 2015, 48(10): 2 007-2 016.
[25] 林玲. 一年两收栽培模式下摘心和生长调节剂对 '夏黑'葡萄花芽分化规律影响的研究[D]. 南宁:广西大学硕士论文, 2017.
LIN Ling. Study on effects of summer pruning and growth regulators on the flower bud differentiation of grape cv. 'Summer black' under cultivation model [D]. Master Dissertation. Guangxi University, Nanning.2017.
[26] Buttrose, M. S. Fruitfulness in grapevines: Effects of light intensity and temperature. Botanical gazette, 1969,130(3):166-173.
[27] 谢计蒙,王海波,王孝娣,等. 设施葡萄品种连年丰产能力与光合生理特性关系研究.[J].果树学报, 2012, 29 (5) :843-851
XIE Ji-meng, WANG Hai-bo, WANG Xiao-di, et al. Relationship between the ability of sustainable productivity and photosynthetic indexes of grape cultivars in greenhouse [J]. Journal of Fruit Science, 2012, 29 (5) :843-851
An Anatomical Study on Flower Bud Differentiation