Distribution of 13 Rice Blast Resistance Genes from Bred Varieties in Xinjiang

doi:10.6048/j.issn.1001-4330.2017.09.004

Abstract

Abstract: 【Objective】 To make clear the distribution situation of rice blast resistance genes from the main bred rice varieties in Xinjiang and provide the basis for rice blast resistance breeding.【Method】In this study, 31 Xinjiang bred rice varieties were taken as materials, specific molecular markers of 13 rice blast resistance genes were used to carry out PCR identification, thus preliminarily establishing the distribution situation of resistance genes from Xinjiang main bred varieties.【Result】There were no Pi21 genes in all the test materials; The proportion of Pi9, Pita, Pib and Pikm were less, which belong to broad spectrum resistance genes, accounting for 9.68%, 22.58%, 6.45% and 12.90%, respectively. However, Pi2, Pigm, Pil, Pikh/Pi54 and Pi63 that belong to broad spectrum resistance genes and Pia, Pid2, Pid3/Pi25 that belong to physiological races specific resistance genes, all existed widely between 67.74% and 100%. The number of blast resistance genes in different varieties were between 5 and 11. Xindao 23 contained 11blast resistance genes that polymerized all blast resistance genes except for Pi21 and Pid2.【Conclusion】Pi2, Pigm, Pil, Pikh/Pi54 and Pi63 that belong to broad spectrum blast resistance genes and Pia, Pid2 and Pid3/Pi25 that belong to physiological races specific resistance genes were widely spread in partial bred rice varieties in Xinjiang. The presence and distribution situation of blast resistance genes in varieties can be identified rapidly and efficiently by using specific molecular markers.

Key words: rice; bred varieties; rice blast; resistance genes

摘要： 【目的】明确新疆主要育成水稻品种中稻瘟病抗性基因的分布状况,为稻瘟病抗性育种提供依据。【方法】研究以31份新疆选育的水稻品种为材料,利用13个抗稻瘟病基因的特异性分子标记,进行PCR鉴定,初步建立新疆主要育成品种的抗性基因分布情况。【结果】31份供试材料中均无Pi21基因存在;表现为广谱抗性的Pi9、Pita、Pib和Pikm基因所占比例偏少,分别占9.68%、22.58%、6.45%、12.90%;表现为广谱抗性的Pi2、Pigm、Pil、Pikh/Pi54、Pi63和表现为生理小种特异性抗性的Pia、Pid2、Pid3/Pi25基因广泛存在,所占比率67.74%~100%;不同水稻品种抗性基因数量分布在5~11个,含11个抗性基因的品种是新稻23号,其聚合了除Pi21和Pid2外其余所有抗性基因。【结论】新疆主要水稻育成品种中广泛存在具有光谱抗性的Pi2、Pigm、Pil、Pikh/Pi54、Pi63和表现为生理小种特异性抗性的Pia、Pid2、Pid3/Pi25基因,利用特异性分子标记可以有效快速鉴定品种中的抗性基因的存在和分布情况。

关键词: 水稻, 育成品种, 稻瘟病, 抗性基因

CLC Number:

S511

ZHANG Yan-hong, JIA Chun-ping, WEN Xiao-rong, TANG Fu-sen, WANG Feng-bin, ZHU Xiao-xia. Distribution of 13 Rice Blast Resistance Genes from Bred Varieties in Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(9): 1595-1605.

张燕红, 贾春平, 文孝荣, 唐福森, 王奉斌, 朱小霞. 新疆水稻主要育成品种13个稻瘟病的抗性基因分布[J]. 新疆农业科学, 2017, 54(9): 1595-1605.

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