Responses of Seeds of Different Fragrant Rice Varieties to NaCl Stress in Germination and Seedling Stages

doi:10.6048/j.issn.1001-4330.2022.04.006

Abstract

Abstract:

【Objective】 To screen the salt tolerant varieties suitable for the salinized rice area in Xinjiang by exploring the salt tolerance of different fragrant rice cultivars.【Method】 The effects of different NaCl concentrations (0, 3, 6, 9 and 12 g/L) on seed germination and seedling growth of 20 aromatic rice varieties were studied.【Result】 The results showed that the germination potential, germination rate, shoot length, root length, root number, survival rate and leaf survival rate of different aroma rice varieties all decreased with the increase of NaCl concentrations. Medium salt concentration and high salt concentration (9 g/L, 12 g/L) significantly inhibited the seed germination, reduced the seed germination potential and its germination rate, shortened the length of shoots and roots, and decreased the root number, resulting in an increase in the salt damage index; The salt tolerance performance of different rice materials was different under the same salt concentration treatment.【Conclusion】 Through comprehensive analysis, it was concluded that the suitable NaCl salt concentration for screening at the bud stage and seedling stage of aroma-type rice varieties could be 6 g/L, exceeding the concentration would be detrimental to the germination and seedling growth of rice; The Liangxiang 6-4 fragrance variety has good comprehensive salt tolerance at the bud stage and seedling stage, 97-4-3, Liangxiang 2-1, Xincejing 1, Xinjingxiang 1, Xinjiangxiang 83 and Xinjiangxiang 8 have strong salt tolerance at the bud stage, and the above fragrant rice varieties can be used as salt-tolerant fragrant rice varieties or resources.

Key words: salt stress; aroma rice; germination rate; salt damage index; salt tolerance

摘要：

【目的】研究不同香稻品种的耐盐性,筛选出适应新疆盐渍化稻区的香稻耐盐品种。【方法】分析不同NaCl浓度(0、3、6、9和12 g/L)对20个香型水稻品种种子萌发和幼苗生长的影响。【结果】不同香稻品种的发芽势、发芽率、芽长、根长、根数、成活率及叶片成活率均随着NaCl浓度的增加呈下降的趋势;中、高盐浓度(9、12 g/L)显著抑制种子的萌发,降低种子的发芽势和发芽率,缩短芽长和根长,减少根数,致使盐害指数增加;同一盐浓度处理下,不同水稻材料的耐盐表现有差异。【结论】香型水稻品种的芽期和苗期的适宜筛选NaCl盐浓度可为6 g/L,超过其浓度对水稻的萌发及苗期生长均不利;粮香6-4香型品种的芽期和苗期的综合耐盐性好,97-4-3、粮香2-1、新策粳1号、新粳香1号、新粳香83号和新粳香8号的芽期耐盐性强,以上香稻品种可作为耐盐的香稻品种或资源进行利用。

关键词: 盐胁迫, 香型水稻, 发芽率, 盐害指数, 耐盐性

CLC Number:

S511

DU Xiaojing, ZHANG Yanhong, LÜ Yuping, YUAN Jie, LI Dong, ZHAO Zhiqiang, Buhaliqem Abliz, WANG Fengbin. Responses of Seeds of Different Fragrant Rice Varieties to NaCl Stress in Germination and Seedling Stages[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 827-838.

杜孝敬, 张燕红, 吕玉平, 袁杰, 李冬, 赵志强, 布哈丽且木·阿不力孜, 王奉斌. 不同香稻品种种子萌发和苗期对NaCl胁迫的响应[J]. 新疆农业科学, 2022, 59(4): 827-838.

Figures/Tables 12

Table 1 The number and name of tested materials

序号 No.	品种 Varieties	序号 No.	品种 Varieties
1	97-4-3	11	新策粳1号
2	芳香8号	12	新稻50号
3	粮香2-1	13	新粳香1号
4	粮香6-4	14	新粳香2号
5	粮香7号	15	新粳香5号
6	粮香8号	16	新粳香53号
7	农源香3号	17	新粳香7号
8	松粳香22号	18	新粳香8号
9	天隆香1号	19	新粳香83号
10	五优稻4号	20	新粳伊20号

Table 2 Salt resisting level

耐盐级别 Grade	相对盐害率 Relative salt damage rate(%)	耐盐性 Salt resistance
1	020	极强
3	2040	强
5	4060	中
7	6080	弱
9	80100	极弱

Table 3 of salt damage

耐盐级别 Grade	盐害症状 Salt injury symptoms	耐盐性 Salt resistance
1	生长分蘖基本正常,叶片无受害症状	极强
3	生长分蘖近正常,但叶尖或上部叶片1/2 发白或卷曲;或生长分蘖受抑制, 有些叶片卷曲	强
5	生长分蘖严重抑制,多数叶片卷曲, 仅少数叶片伸长	中
7	生长分蘖停止,多数叶片干枯	弱
9	植株死亡或接近死亡	极弱

Table 4 Evaluation standard of salt tolerance based on salt damage index

耐盐级别 Grade	盐害指数 Salt damage index(%)	耐盐性 Salt resistance
1	015.0	极强
3	15.130.0	强
5	30.160.0	中
7	60.185.0	弱
9	85.1100.0	极弱

Table 5 Comparison of germination potential of aroma rice seeds with different salt concentrations

品种 Varieties	CK处理 0 g/L (%)	T₁处理 3 g/L (%)	T₂处理 6 g/L (%)	T₃处理 9 g/L (%)	T₄处理 12 g/L (%)
97-4-3	92.00	86.67	82.67	69.33	52.67
芳香8号	89.33	83.33	82.00	25.33	6.67
粮香2-1	94.00	88.67	87.33	62.00	24.67
粮香6-4	90.00	76.00	71.33	56.67	10.67
粮香7号	82.00	74.67	60.67	48.00	1.33
粮香8号	90.67	88.00	80.00	51.33	45.33
农源香3号	81.33	71.33	56.67	13.33	5.33
松粳香22号	86.67	78.67	66.67	32.00	4.00
天隆香1号	96.67	88.00	74.00	26.67	6.67
五优稻4号	87.33	64.67	52.67	22.00	5.33
新策粳1号	92.67	88.67	87.33	54.67	38.67
新稻50号	86.67	84.67	66.67	31.33	26.00
新粳香1号	93.33	92.00	90.67	26.67	19.33
新粳香2号	79.33	72.67	56.67	10.00	6.67
新粳香5号	89.33	86.67	78.67	44.00	39.33
新粳香53号	85.33	74.00	70.67	31.33	15.33
新粳香7号	83.33	64.67	70.67	9.33	6.67
新粳香8号	92.67	87.33	74.67	38.67	7.33
新粳香83号	94.00	92.00	87.33	78.67	40.00
新粳伊20号	86.00	85.33	72.67	38.00	19.33
平均值	88.63^a	81.40^ab	73.50^b	38.47^c	19.07^d

Table 6 Comparison of germination rate of aroma rice seeds with different salt concentrations

品种 Varieties	CK处理 0 g/L (%)	T₁处理 3 g/L (%)	相对受害率 (%)	T₂处理 6 g/L (%)	相对受害率 (%)	T₃处理 9 g/L (%)	相对受害率 (%)	T₄处理 12 g/L (%)	相对受害率 (%)
97-4-3	96.67	95.33	1.38	92.00	4.83	85.00	12.07	80.00	17.24
芳香8号	96.67	96.67	0.00	94.67	2.07	66.00	31.72	34.00	64.83
粮香2-1	99.33	99.33	0.00	98.67	0.67	86.33	13.09	82.67	16.78
粮香6-4	98.00	98.00	0.00	96.00	2.04	70.67	27.89	42.00	57.14
粮香7号	91.33	86.67	5.11	86.67	5.11	62.00	32.12	21.33	76.64
粮香8号	99.33	96.00	3.36	92.00	7.38	66.67	32.89	55.33	44.30
农源香3号	94.00	84.00	10.64	75.33	19.86	55.33	41.13	22.00	76.60
松粳香22	96.00	93.33	2.78	86.67	9.72	60.67	36.81	14.00	85.42
天隆香1号	99.33	97.33	2.01	93.33	6.04	71.33	28.19	35.33	64.43
五优稻4号	96.67	92.67	4.14	83.33	13.79	62.67	35.17	24.67	74.48
新策粳1号	98.67	97.33	1.35	96.00	2.70	86.67	12.16	82.67	16.22
新稻50号	96.67	96.67	0.00	86.67	10.34	66.67	31.03	39.33	59.31
新粳香1号	98.67	97.33	1.35	96.33	2.36	86.00	12.84	83.33	15.54
新粳香2号	91.33	91.33	0.00	73.33	19.71	49.33	45.99	23.33	74.45
品种 Varieties	CK处理 0 g/L (%)	T₁处理 3 g/L (%)	相对受害率 (%)	T₂处理 6 g/L (%)	相对受害率 (%)	T₃处理 9 g/L (%)	相对受害率 (%)	T₄处理 12 g/L (%)	相对受害率 (%)
新粳香5号	98.67	93.33	5.41	92.00	6.76	72.00	27.03	40.67	58.78
新粳香53号	93.33	90.00	3.57	82.67	11.43	52.00	44.29	32.67	65.00
新粳香7号	95.33	94.00	1.40	86.67	9.09	66.00	30.77	32.00	66.43
新粳香8号	98.67	96.67	2.03	95.33	3.38	83.33	15.54	72.00	27.03
新粳香83号	98.00	94.67	3.40	94.00	4.08	90.67	7.48	80.00	18.37
新粳伊20号	94.00	92.00	2.13	88.67	5.67	68.67	26.95	36.00	61.70
平均值	96.53^a	94.13^a	2.50	89.52^a	7.35	70.40^b	27.26	46.67^c	52.03

Fig.1 Effects of different salt concentrations on average and average germination rate of 20 aroma rice seeds

Table 7 Comparison of seed sprout length, root length and root number of aroma rice with different salt concentrations

品种 Varieties	芽长Sprout length(cm)					根长Root length(cm)					根数(根)Number of roots
品种 Varieties	CK	T₁	T₂	T₃	T₄	CK	T₁	T₂	T₃	T₄	CK	T₁	T₂	T₃	T₄
97-4-3	4.56	3.81	3.20	2.31	1.94	2.57	1.75	0.66	0.25	0.25	6.80	6.83	4.17	3.37	1.13
芳香8号	6.28	6.00	3.69	2.28	1.14	6.25	7.87	2.77	0.56	0.00	6.11	6.40	4.80	2.70	0.00
粮香2-1	5.23	3.78	3.03	2.13	1.26	3.44	2.56	0.36	0.06	0.00	7.10	7.00	3.37	0.13	0.00
粮香6-4	5.23	3.87	2.73	2.29	1.32	2.57	2.53	0.69	0.27	0.03	6.13	6.20	4.40	1.90	0.20
粮香7号	4.54	3.47	2.75	1.83	1.31	1.56	1.80	0.50	0.21	0.12	5.50	7.50	3.07	1.43	1.23
粮香8号	4.46	3.65	3.11	2.51	1.85	2.90	2.92	2.09	0.80	0.36	6.13	6.50	4.57	3.07	3.13
农源香3号	6.33	5.16	2.85	2.20	1.38	6.12	7.30	2.81	1.02	0.36	5.90	6.30	4.08	2.70	1.73
松粳香22	6.03	4.38	3.40	1.98	1.16	6.45	3.65	1.72	1.20	0.07	5.77	6.17	3.60	2.17	0.73
天隆香1号	4.68	3.41	2.31	1.87	0.79	3.34	2.34	0.63	0.21	0.04	7.73	5.60	3.73	1.43	0.20
五优稻4号	6.57	5.40	3.47	2.16	1.45	6.13	6.61	2.25	0.65	0.18	4.73	6.63	5.00	2.73	1.20
新策粳1号	5.38	3.92	3.33	2.60	1.85	3.50	2.50	1.05	0.56	0.22	6.63	7.50	4.13	2.80	2.40
新稻50号	4.82	4.08	2.36	2.62	1.88	3.58	3.17	0.51	1.12	0.27	5.60	6.10	3.10	2.53	2.17
新粳香1号	7.80	5.76	2.86	2.29	1.31	3.95	2.67	1.38	0.73	0.00	6.25	6.07	3.83	2.17	0.00
新粳香2号	4.44	4.21	2.67	2.36	1.39	6.65	8.18	2.08	1.09	0.14	6.67	6.30	3.23	2.93	1.37
新粳香5号	5.02	4.92	3.43	2.72	2.36	6.47	11.30	3.10	1.11	0.57	5.07	5.50	5.47	3.00	2.57
新粳香53号	6.30	4.81	3.11	1.96	1.37	2.58	5.08	0.77	0.38	0.17	8.07	6.70	3.07	2.23	1.37
新粳香7号	4.99	4.83	2.28	1.66	0.85	7.48	6.63	0.79	0.28	0.02	6.67	7.47	4.13	2.13	0.10
新粳香8号	6.12	5.14	3.32	2.94	1.43	2.63	2.86	1.09	0.63	0.19	7.07	7.93	4.50	3.30	1.17
新粳香83号	6.52	4.91	2.70	2.06	1.52	2.57	2.59	0.88	0.15	0.16	7.37	6.70	4.53	1.07	2.37
新粳伊20号	5.02	4.98	3.45	3.09	2.10	8.13	9.60	2.93	1.57	0.35	6.27	5.37	4.67	3.13	1.55
平均值	5.52^a	4.52^b	3.00^c	2.29^d	1.48^e	4.44^a	4.69^a	1.45^b	0.6 ${4^{b}}^{c}$	0.18^c	6.38^a	6.54^a	4.07^b	2.35^c	1.23^d

Table 7 Comparison of seed sprout length, root length and root number of aroma rice with different salt concentrations

品种 Varieties	芽长Sprout length(cm)					根长Root length(cm)					根数(根)Number of roots
品种 Varieties	CK	T₁	T₂	T₃	T₄	CK	T₁	T₂	T₃	T₄	CK	T₁	T₂	T₃	T₄
97-4-3	4.56	3.81	3.20	2.31	1.94	2.57	1.75	0.66	0.25	0.25	6.80	6.83	4.17	3.37	1.13
芳香8号	6.28	6.00	3.69	2.28	1.14	6.25	7.87	2.77	0.56	0.00	6.11	6.40	4.80	2.70	0.00
粮香2-1	5.23	3.78	3.03	2.13	1.26	3.44	2.56	0.36	0.06	0.00	7.10	7.00	3.37	0.13	0.00
粮香6-4	5.23	3.87	2.73	2.29	1.32	2.57	2.53	0.69	0.27	0.03	6.13	6.20	4.40	1.90	0.20
粮香7号	4.54	3.47	2.75	1.83	1.31	1.56	1.80	0.50	0.21	0.12	5.50	7.50	3.07	1.43	1.23
粮香8号	4.46	3.65	3.11	2.51	1.85	2.90	2.92	2.09	0.80	0.36	6.13	6.50	4.57	3.07	3.13
农源香3号	6.33	5.16	2.85	2.20	1.38	6.12	7.30	2.81	1.02	0.36	5.90	6.30	4.08	2.70	1.73
松粳香22	6.03	4.38	3.40	1.98	1.16	6.45	3.65	1.72	1.20	0.07	5.77	6.17	3.60	2.17	0.73
天隆香1号	4.68	3.41	2.31	1.87	0.79	3.34	2.34	0.63	0.21	0.04	7.73	5.60	3.73	1.43	0.20
五优稻4号	6.57	5.40	3.47	2.16	1.45	6.13	6.61	2.25	0.65	0.18	4.73	6.63	5.00	2.73	1.20
新策粳1号	5.38	3.92	3.33	2.60	1.85	3.50	2.50	1.05	0.56	0.22	6.63	7.50	4.13	2.80	2.40
新稻50号	4.82	4.08	2.36	2.62	1.88	3.58	3.17	0.51	1.12	0.27	5.60	6.10	3.10	2.53	2.17
新粳香1号	7.80	5.76	2.86	2.29	1.31	3.95	2.67	1.38	0.73	0.00	6.25	6.07	3.83	2.17	0.00
新粳香2号	4.44	4.21	2.67	2.36	1.39	6.65	8.18	2.08	1.09	0.14	6.67	6.30	3.23	2.93	1.37
新粳香5号	5.02	4.92	3.43	2.72	2.36	6.47	11.30	3.10	1.11	0.57	5.07	5.50	5.47	3.00	2.57
新粳香53号	6.30	4.81	3.11	1.96	1.37	2.58	5.08	0.77	0.38	0.17	8.07	6.70	3.07	2.23	1.37
新粳香7号	4.99	4.83	2.28	1.66	0.85	7.48	6.63	0.79	0.28	0.02	6.67	7.47	4.13	2.13	0.10
新粳香8号	6.12	5.14	3.32	2.94	1.43	2.63	2.86	1.09	0.63	0.19	7.07	7.93	4.50	3.30	1.17
新粳香83号	6.52	4.91	2.70	2.06	1.52	2.57	2.59	0.88	0.15	0.16	7.37	6.70	4.53	1.07	2.37
新粳伊20号	5.02	4.98	3.45	3.09	2.10	8.13	9.60	2.93	1.57	0.35	6.27	5.37	4.67	3.13	1.55
平均值	5.52^a	4.52^b	3.00^c	2.29^d	1.48^e	4.44^a	4.69^a	1.45^b	0.6 ${4^{b}}^{c}$	0.18^c	6.38^a	6.54^a	4.07^b	2.35^c	1.23^d

Fig. 2 Effects of Different Salt Concentrations on Average Shoot Length, Average Root Length and Average Root Number of 20 Aroma Type Rice

Fig.3 Effects of different salt concentrations on average seedling survival rate of 20 aroma type rice

Table 8 Comparison of seedling survival rate and leaf survival rate of aroma rice with different salt concentrations

品种 Varieties	幼苗成活率 Seedling survival rate(%)					叶片成活率 Leaf survival rate(%)
品种 Varieties	CK	T₁	T₂	T₃	T₄	CK	T₁	T₂	T₃	T₄
97-4-3	100.00	100.00	54.55	25.56	0.00	/	/	28.57	/	/
芳香8号	100.00	93.75	65.63	31.25	0.00	/	/	11.43	/	/
粮香2-1	100.00	100.00	71.39	4.17	0.00	/	/	25.71	/	/
粮香6-4	100.00	100.00	74.77	41.67	0.00	/	/	25.71	/	/
粮香7号	100.00	87.50	56.25	0.00	0.00	/	/	20.00	/	/
粮香8号	100.00	92.86	34.23	22.22	0.00	/	/	31.43	/	/
农源香3号	100.00	83.75	43.97	6.25	0.00	/	/	17.14	/	/
松粳香22	100.00	100.00	38.76	18.75	0.00	/	/	22.86	/	/
天隆香1号	100.00	95.83	57.64	5.56	0.00	/	/	28.57	/	/
五优稻4号	100.00	88.89	35.89	0.00	0.00	/	/	34.29	/	/
新策粳1号	100.00	95.00	72.02	15.34	0.00	/	/	25.71	/	/
新稻50号	100.00	89.44	72.02	31.25	0.00	/	/	31.43	/	/
新粳香1号	100.00	100.00	43.98	10.10	0.00	/	/	20.00	/	/
新粳香2号	100.00	85.24	20.42	0.00	0.00	/	/	8.57	/	/
新粳香5号	100.00	100.00	79.97	11.11	0.00	/	/	28.57	/	/
新粳香53号	100.00	90.00	39.17	0.00	0.00	/	/	31.43	/	/
新粳香7号	100.00	94.44	50.56	10.10	0.00	/	/	22.86	/	/
新粳香8号	100.00	100.00	47.73	0.00	0.00	/	/	37.14	/	/
新粳香83号	100.00	100.00	63.90	18.34	0.00	/	/	31.43	/	/
新粳伊20号	100.00	100.00	70.49	17.50	0.00	/	/	34.29	/	/
平均值	100.00^a	94.84^a	54.67^b	13.46^c	0.00^d	/	/	25.86	/	/

Table 9 Comparison of salt damage index and salt tolerance level of aroma rice seedlings with different salt concentrations

品种 Varieties	CK			T₁		T₂		T₃		T₄
品种 Varieties	盐害指数 (%)	耐盐级别		盐害指数(%)	耐盐级别	盐害指数(%)	耐盐级别	盐害指数(%)	耐盐级别	盐害指数(%)	耐盐级别
97-4-3	0.00		0	13.13	1	52.65	5	69.11	7	100.00	9
芳香8号	0.00		0	13.33	1	49.89	5	66.52	7	100.00	9
粮香2-1	0.00		0	26.98	3	47.67	5	79.22	7	100.00	9
粮香6-4	0.00		0	12.96	1	44.63	5	59.59	5	100.00	9
粮香7号	0.00		0	29.11	3	51.62	5	100.00	9	100.00	9
粮香8号	0.00		0	12.96	1	62.65	7	71.59	7	100.00	9
农源香3号	0.00		0	29.33	3	55.00	5	78.30	7	100.00	9
松粳香22	0.00		0	23.89	3	56.82	7	73.22	7	100.00	9
天隆香1号	0.00		0	23.33	3	50.56	5	78.44	7	100.00	9
五优稻4号	0.00		0	25.00	3	61.67	7	100.00	9	100.00	9
新策粳1号	0.00		0	19.58	3	47.00	5	75.06	7	100.00	9
新稻50号	0.00		0	22.22	3	46.43	5	66.48	7	100.00	9
新粳香1号	0.00		0	17.33	3	55.00	5	76.96	7	100.00	9
新粳香2号	0.00		0	29.63	3	69.07	7	100.00	9	100.00	9
新粳香5号	0.00		0	13.09	1	42.89	5	76.78	7	100.00	9
新粳香53号	0.00		0	13.89	1	56.11	5	100.00	9	100.00	9
新粳香7号	0.00		0	23.33	3	52.78	5	76.93	7	100.00	9
新粳香8号	0.00		0	13.33	1	54.44	5	100.00	9	100.00	9
新粳香83号	0.00		0	14.29	1	50.00	5	73.44	7	100.00	9
新粳伊20号	0.00		0	13.58	1	48.74	5	73.44	7	100.00	9
平均值	0.00^e		0	19.52^d	2.10	52.78^c	5.30	79.75^b	7.40	100.00^a	9.00

References 27

[1]	周文期, 强晓霞, 王森, 等. OsLPL2/PIR基因抗旱耐盐机制研究[J/OL]. 作物学报: 1-17 [2022-01-29]. http://kns.cnki.net/kcms/detail/11.1809.s.20211117.2106.006.html.
	ZHOU Wenqi, QIANG Xiaoxia, WANG Sen, et al. Mechanism of drought and salt tolerance of Os LPL2/PIR gene in rice[J/OL]. Acta Agronomica Sinica, 1-17[2022-01-29]. http://kns.cnki.net/kcms/detail/11.1809.s.20211117.2106.006.html.
[2]	苏京志, 温敏, 丁一汇, 等. 全球变暖趋缓研究进展[J]. 大气科学, 2016, 40(6):1143-1153.
	SU Jingzhi, WEN Min, DING Yihui, et al. Hiatus of global warming: A review[J]. Atmospheric Sciences, 2016, 40(6):1143-1153.
[3]	Farooq M, Wahid A, Lee D J, et al. Advances in Drought Resistance of Rice[J]. Critical Reviews in Plant Sciences, 2009, 28(4):199-217. DOI URL
[4]	魏征, 屠乃美, 易镇邪. 盐碱地对水稻的胁迫效应及其改良与高效利用的研究进展[J]. 湖南生态科学学报, 2019, 6(4):45-52.
	WEI Zheng, TU Naimei, YI Zhenxie. Research Progress on Stress Effect of Saline-alkali Soil on Rice and Its Improvement and Efficient Utilization[J]. Journal of Hunan Ecological Science, 2019, 6(4):45-52.
[5]	饶玉春, 戴志俊, 朱怡彤, 等. 水稻抗干旱胁迫的研究进展[J]. 浙江师范大学学报(自然科学版), 2020, 43(4):417-429.
	RAO Yuchun, DAI Zhijun, ZHU Yitong, et al. Advances in research of drought resistance in rice[J]. Journal of Zhejiang Normal University (Natural Sciences Ed.), 2020, 43(4):417-429.
[6]	Mahbod S, Hanafi M M, Rafii M Y, et al. Improvement of Drought Tolerance in Rice ( Oryza sativa L.): Genetics, Genomic Tools, and the WRKY Gene Family[J]. Journal of Biomedicine and Biotechnology, 2018:1-20.
[7]	Farifteh J, Farshad A, George R J. Assessing salt-affected soils using remote sensing, solute modelling, and geophysics[J]. Geoderma, 2006, 130(3-4):191-206. DOI URL
[8]	Raiesi F, Razmkhah M, Kiani S. Salinity stress accelerates the effect of cadmium toxicity on soil N dynamics and cycling: Does joint effect of these stresses matter[J]. Ecotoxicol Environ Saf, 2018, 153(MAY):160-167. DOI URL
[9]	王琳琳, 李素艳, 孙向阳, 等. 不同隔盐材料对滨海盐渍土水盐动态和树木生长的影响[J]. 水土保持通报, 2015, 35(4):141-147,151.
	WANG Linlin, LI Suyan, SUN Xiangyang, et al. Effect of salt-isolation materials on soli water and salt movement and tree growth of saline soil in a coastal region[J]. Bulletin of Soil and Water Conservation, 2015, 35(4):141-147,151.
[10]	Hussain S, Zhang J, Zhong C, et al. Effects of salt stress on rice growth, development characteristics, and the regulating ways: A review[J]. Journal of Integrative Agriculture, 2017, 16(11):2357-2374. DOI URL
[11]	Mishra A K, Mottaleb K A, Khanal A R, et al. Abiotic stress and its impact on production efficiency: The case of rice farming in Bangladesh[J]. Agriculture Ecosystems & Environment, 2015, 199:146-153. DOI URL
[12]	祝一文, 赵方贵, 成云峰, 等. ‘海稻86’耐盐碱胁迫生理机制的初步研究[J]. 青岛农业大学学报(自然科学版), 2018, 35(1):32-39.
	ZHU Yiwen, ZHAO Fanggui, CHENG Yunfeng, et al. The preliminary study on alkali-salt tolerance of ‘sea rice 86’ and physiological mechanisms[J]. Journal of Qingdao Agricultural University (Natural Science Ed.), 2018, 35(1):32-39.
[13]	魏征, 邹燕, 陈澎军, 等. 不同类型水稻芽期的耐盐性差异[J]. 湖南农业大学学报(自然科学版), 2021, 47(3):254-261,316.
	WEI Zheng, ZOU Yan, CHEN Pengjun, et al. Analysis on the difference of salt tolerance of different types of rice at budding stage[J]. Journal of Hunan Agricultural University (Natural Sciences Ed.), 2021, 47(3):254-261,316.
[14]	冯钟慧, 刘晓龙, 姜昌杰, 等. 吉林省粳稻种质萌发期耐碱性和耐盐性综合评价[J]. 土壤与作物, 2016, 5(2):120-127.
	FENG Zhonghui, LIU Xiaolong, JIANG Changjie, et al. Comprehensive Evaluation of Rice (Oryza sativa japonica) Germplasm for Alkaline / Saline Tolerance at Germination Stage from Jilin Province, China[J]. Soils and Crops, 2016, 5(2):120-127.
[15]	王奉斌, 张燕红, 袁杰, 等. 新疆耐盐水稻种质资源的筛选[J]. 新疆农业科学, 2009, 46(3):501-505.
	WANG Fengbin, ZHANG Yanhong, YUAN Jie, et al. Screening of germplasm resources of salt-resisting rice in Xinjiang[J]. Xinjiang Agricultural Sciences, 2009, 46(3):501-505.
[16]	张燕红, 袁杰, 王奉斌. 不同新疆水稻品种种子发芽耐盐性的比较[J]. 安徽农学通报(上半月刊), 2009, 15(3):102-103.
	ZHANG Yanhong, YUAN Jie, WANG Fengbin. Comparison of salt tolerance in Seed Germination of different rice varieties in Xinjiang[J]. Anhui Agricultural Science Bulletin, 2009, 15(3):102-103.
[17]	袁杰, 王学强, 张燕红, 等. 水稻种质资源苗期耐盐性鉴定[J]. 分子植物育种, 2020, 18(20):6808-6814.
	YUAN Jie, WANG Xueqiang, ZHANG Yanhong, et al. Identification and Screening of Salt Tolerance in Rice Germplasm Resources at Seedling Stage[J]. Molecular Plant Breeding, 2020, 18(20):6808-6814.
[18]	贺奇, 王昕, 马洪文, 等. 盐胁迫下粳稻种子发芽特性及耐盐性评价[J]. 中国稻米, 2018, 24(1):28-32.
	HE Qi, WANG Xin, MA Hongwen, et al. Germination Characteristics and Salt Tolerance Evaluation of Japonica Rice Seeds under Salt Stress[J]. China Rice, 2018, 24(1):28-32.
[19]	杨娅坤, 赵飞, 刘建, 等. 盐碱胁迫对水稻的影响及其相关机制的研究进展[J/OL]. 分子植物育种: 1-17 [2022-01-29]. http://kns.cnki.net/kcms/detail/46.1068.S.20210315.0838.004.html.
	YANG Yakun, ZHAO Fei, LIU Jian, et al. Research Progress of Saline-alkali Stress on Rice and its Related Mechanism[J/OL]. Molecular Plant Breeding: 1-17 [2022-01-29]. http://kns.cnki.net/kcms/detail/46.1068.S.20210315.0838.004.html.
[20]	王志欣, 邹德堂, 刘华龙, 等. 东北粳稻芽期耐盐碱性差异研究[J]. 黑龙江农业科学, 2012,(8):6-11.
	WANG Zhixin, ZOU Detang, LIU Hualong, et al. Study on the difference of the alkaline tolerance of northeast Japonica rice at germination stage[J]. Heilongjiang Agricultural Sciences, 2012,(8):6-11.
[21]	曾泳怡, 冯梓晴, 曾晓靖, 等. NaCl胁迫对不同基因型水稻种子萌发和幼苗生长的影响[J]. 安徽农业科学, 2021, 49(7):25-29.
	ZENG Yongyi, FENG Ziqing, ZENG Xiaojing, et al. Effects of NaCl Stress on Seed Germination and Seedling Growth of Different Rice Genotypes[J]. Journal of Anhui Agricultural Sciences, 2021, 49(7):25-29.
[22]	贾宝艳, 周婵婵, 孙晓雪, 等. 辽宁省水稻种质资源的耐盐性鉴定评价[J]. 作物杂志, 2013,(4):57-62.
	JIA Baoyan, ZHOU Chanchan, SUN Xiaoxue, et al. The Evaluation of Salt Tolerance Ability of Rice Varieties in Liaoning Province[J]. Crops, 2013,(4):57-62.
[23]	金梦野, 李小华, 李昉泽, 等. 盐碱复合胁迫对水稻种子发芽的影响[J]. 中国生态农业学报(中英文), 2020, 28(4):566-574.
	JIN Mengye, LI Xiaohua, LI Fangze, et al. Effects of mixed saline-alkali stress on germination of rice[J]. Chinese Journal of Eco-Agriculture, 2020, 28(4):566-574.
[24]	袁驰, 梁永霞, 刘静, 等. 复盐胁迫对不同水稻品种种子萌发和幼苗生长的影响[J]. 种子, 2018, 37(6):78-81.
	YUAN Chi, LIANG Yongxia, LIU Jing, et al. Effect of compound saline stress on seed germination and seedling growth of different rice varieties[J]. Seed, 2018, 37(6):78-81.
[25]	潘晓飚, 谢留杰, 陈剑, 等. 盐胁迫下杂交水稻种子发芽特性及耐盐性评价[J]. 中国农学通报, 2014, 30(21):75-79.
	PAN Xiaobiao, XIE Liujie, CHEN Jian, et al. Germination characteristics of hybrid rice seeds under salt stress and their evaluation of salt-tolerance[J]. Chinese Agricultural Science Bulletin, 2014, 30(21):75-79.
[26]	陈剑, 潘晓飚, 谢留杰, 等. 盐胁迫对水稻恢复系发芽的影响[J]. 浙江农业科学, 2014,(4):471-473.
	CHEN Jian, PAN Xiaobiao, XIE Liujie, et al. Effects of salt stress on Germination of Rice Restorer Lines[J]. Journal of Zhejiang Agricultural Sciences, 2014,(4):471-473.
[27]	张治振, 李稳, 周起先, 等. 不同水稻品种幼苗期耐盐性评价[J]. 作物杂志, 2020,(3):92-101.
	ZHANG Zhizhen, LI Wen, ZHOU Qixian, et al. Salt tolerance evaluation of different rice varieties at seedling stage[J]. Crops, 2020,(3):92-101.