盐胁迫下接种AM真菌对野生无芒雀麦生理的影响

doi:10.6048/j.issn.1001-4330.2025.01.016

摘要/Abstract

摘要：

【目的】 研究野生无芒雀麦分别接种2种不同的AM真菌,探究盐胁迫下接种AM真菌对野生无芒雀麦生理的影响。【方法】 在NaCl浓度分别为0%、0.4%、0.6%和0.8%盐胁迫条件下,分析接种AM菌种的植株与不接种AM真菌的野生无芒雀麦的耐盐性。【结果】 接种AM真菌均能提高野生无芒雀麦的株高、茎粗、地下部分干重无地上部分干重;接种AM真菌可显著地提高野生无芒雀麦的抗氧化酶(SOD、POD、CAT)活性,并可提高可溶性糖和脯氨酸的含量;随着盐浓度的上升,接种AM真菌MDA的含量明显小于不接种AM真菌的含量。【结论】 接种2种AM真菌均能促进野生无芒雀麦的生长,提高野生无芒雀麦的耐盐性。

关键词: 野生无芒雀麦; 丛枝菌根真菌; 盐胁迫; 生长; 生理

Abstract:

【Objective】 In this study, two different AM fungi were inoculated in wild Bromus inermiss to explore the effects of inoculation with AM fungi under salt stress on the physiology of wild Bromus inermiss. 【Methods】 Under salt stress conditions of 0%, 0.4%, 0.6% and 0.8% NaCl concentration, respectively, whether plants inoculated with AM strains could improve the salt tolerance of wild Bromus inermiss compared with plants not inoculated with AM fungi. 【Results】 Inoculated with AM fungus could increase plant height, stem diameter, dry weight of underground part and dry weight of above ground part of wild Bromus inermiss. Inoculation with AM fungi could significantly improve the activities of antioxidant enzymes (SOD, POD, CAT), and the contents of soluble sugar and proline in wild Bromus inermiss. With the increase of salt concentration, MDA content of inoculated AM fungi was significantly lower than that of uninoculated AM fungi. 【Conclusion】 Inoculation of both AM fungi could promote the growth of wild Bromus inermiss and improve the salt tolerance of wild Bromus inermiss.

Key words: Bromus inermiss; arbuscular mycorrhizal fungi; salt stress; growth; physiology

中图分类号:

S188

弓兆星, 韩鹏程, 李泽森, 李桂真, 王玉祥, 张博. 盐胁迫下接种AM真菌对野生无芒雀麦生理的影响[J]. 新疆农业科学, 2025, 62(1): 129-136.

GONG Zhaoxing, HAN Pengcheng, LI Zesen, LI Guizhen, WANG Yuxiang, ZHANG Bo. The physiological effects of inoculation with AM fungi under salt stress on wild smooth[J]. Xinjiang Agricultural Sciences, 2025, 62(1): 129-136.

图/表 3

图1 盐胁迫下AM真菌下野生无芒雀麦生长的变化注:小写字母表示在盐浓度不变的情况下,不接菌种或接种不同菌种带来的显著性变化;大写字母表示在是否接种菌种或菌种不变的情况下,盐浓度的显著性变化。不同大写小写字母表示在P<0.05水平下的差异,下同

Fig.1 Changes of AM fungi on the growth of Bromus inermiss wheat under salt stress Notes:Lowercase Letters indicate that the salt concentration remains unchanged,Significant changes in the non-vaccinated or vaccinated with no inquiru about the strain;capital letters indicate whether vaccination or strain remains unchanged,significant changes in salt concentration.lowercase shows significantly different at P<0.05,the same as below

图2 盐胁迫下AM真菌下野生无芒雀麦渗透物质调节的变化

Fig.2 Changes of AM fungi on the regulation of osmotic substances in wild Bromus inermiss wheat under salt stress

图3 盐胁迫下AM真菌下野生无芒雀麦渗透物质调节的变化

Fig.3 Changes of AM fungi on the regulation of osmotic substances in Bromus inermisse wheat under salt stress

参考文献 35

[1]	Pan C C, Liu C G, Zhao H L, et al. Changes of soil physico-chemical properties and enzyme activities in relation to grassland salinization[J]. European Journal of Soil Biology, 2013, 55: 13-19.
[2]	王娜, 何俐蓉. 盐渍化土壤的成因及改良材料的应用[J]. 南方农机, 2021, 52(5): 72-73.
	WANG Na, HE Lirong. Causes of salinized soil and application of improved materials[J]. China Southern Agricultural Machinery, 2021, 52(5): 72-73.
[3]	张晓婷, 王雪松, 贾文飞, 等. 植物在盐处理下的研究进展[J]. 北方园艺, 2021,(6): 137-143.
	ZHANG Xiaoting, WANG Xuesong, JIA Wenfei, et al. Research progress of plants under salt treatment[J]. Northern Horticulture, 2021,(6): 137-143.
[4]	张辉, 宋琳, 陈晓琳, 等. 土壤退化的原因与修复作用研究[J]. 海洋科学, 2020, 44(8): 147-161.
	ZHANG Hui, SONG Lin, CHEN Xiaolin, et al. Study on the causes and remediation of soil degradation[J]. Marine Sciences, 2020, 44(8): 147-161.
[5]	杨海霞, 刘润进, 郭绍霞. AM真菌摩西球囊霉对盐胁迫条件下高羊茅生长特性的影响[J]. 草业学报, 2014, 23(4): 195-203. DOI
	YANG Haixia, LIU Runjin, GUO Shaoxia. Effects of arbuscular mycorrhizal fungus Glomus mosseae on the growth characteristics of Festuca arundinacea under salt stress conditions[J]. Acta Prataculturae Sinica, 2014, 23(4): 195-203. DOI
[6]	曾广萍, 张霞, 刘红玲, 等. 盐胁迫下AM真菌对红花耐盐性的影响[J]. 植物生理学报, 2011, 47(11): 1069-1074.
	ZENG Guangping, ZHANG Xia, LIU Hongling, et al. Effect of AM fungi on salt tolerance of Carthamus tinctorius L. under salt stress[J]. Plant Physiology Journal, 2011, 47(11): 1069-1074.
[7]	王发园, 林先贵, 周健民. 中国AM真菌的生物多样性[J]. 生态学杂志, 2004, 23(6): 149-154.
	WANG Fayuan, LIN Xiangui, ZHOU Jianmin. Biodiversity of AM fungi in China[J]. Chinese Journal of Ecology, 2004, 23(6): 149-154.
[8]	冯曾威. 目前最全的从枝菌根真菌基因组(8个菌种)[DB/OL]. https://www.jianshu.com/p/2cbccOed76e8, 2021-06-16/2022-03-01.
	FENG Zengwei. The most complete genome of mycomycorrhizal fungi (8 species)[DB/OL]. https://www.jianshu.com/p/2cbccOed76e8, 2021-06-16/2022-03-01.
[9]	王发园, 刘润进. 黄河三角洲盐碱地的丛枝菌根真菌[J]. 菌物系统, 2002, 21(2): 196-202.
	WANG Fayuan, LIU Runjin. Arbuscular mycorrhizal fungi in saline-alkaline soils of Yellow River Delta[J]. Mycosystema, 2002, 21(2): 196-202.
[10]	王发园, 林先贵, 周健民. 丛枝菌根真菌分类最新进展[J]. 微生物学杂志, 2005, 25(3): 41-45.
	WANG Fayuan, LIN Xiangui, ZHOU Jianmin. Latest advances in the classification of arbuscular mycorrhizal fungi[J]. Journal of Microbiology, 2005, 25(3): 41-45.
[11]	Aliasgharzad N, Ali Bolandnazar S, Neyshabouri M R, et al. Impact of soil sterilization and irrigation intervals on P and K acquisition by mycorrhizal onion (Allium cepa)[J]. Biologia, 2009, 64(3): 512-515.
[12]	Hamilton C E, Bever J D, Labbé J, et al. Mitigating climate change through managing constructed-microbial communities in agriculture[J]. Agriculture, Ecosystems & Environment, 2016, 216: 304-308.
[13]	Rillig M C, Mummey D L. Mycorrhizas and soil structure[J]. The New Phytologist, 2006, 171(1): 41-53.
[14]	Wu N, Li Z, Liu H G, et al. Influence of arbuscular mycorrhiza on photosynthesis and water status of Populus cathayana Rehder males and females under salt stress[J]. Acta Physiologiae Plantarum, 2015, 37(9): 183.
[15]	王玉祥, 杜雨, 陈映霞, 等. 盐胁迫对无芒雀麦种子萌发及苗期生理指标的影响[J]. 干旱区资源与环境, 2022, 36(5): 139-145.
	WANG Yuxiang, DU Yu, CHEN Yingxia, et al. Effects of salt stress on seed germination and seedling physiological indexes of Bromus inermis Leyss[J]. Journal of Arid Land Resources and Environment, 2022, 36(5): 139-145.
[16]	田小霞, 毛培春, 孟林, 等. 无芒雀麦苗期耐盐指标筛选及耐盐性综合评价[J]. 干旱区资源与环境, 2017, 31(10): 156-161.
	TIAN Xiaoxia, MAO Peichun, MENG Lin, et al. Determination of indicators for salt-tolerant evaluation and comprehensive evaluation of salt-tolerant at the seedlings of Bromus inermis[J]. Journal of Arid Land Resources and Environment, 2017, 31(10): 156-161.
[17]	孙铭, 王思琪, 艾尔肯·达吾提, 等. 抗氧化剂引发对无芒雀麦老化种子发芽及幼苗生长的影响[J]. 草业学报, 2019, 28(11): 105-113. DOI
	SUN Ming, WANG Siqi, Aierken Dawuti, et al. Effects of antioxidant priming on germination and seedling growth of aged seeds of smooth bromegrass[J]. Acta Prataculturae Sinica, 2019, 28(11): 105-113. DOI
[18]	郭孝, 郭良兴, 刘党标. 无芒雀麦在单播及混播下牧草产量和品质的分析[J]. 中国草食动物科学, 2018, 38(5): 62-65.
	GUO Xiao, GUO Liangxing, LIU Dangbiao. Analysis of forage yield and quality of Bromus inermis under unicast and mixed sowing[J]. China Herbivore Science, 2018, 38(5): 62-65.
[19]	马玉宝, 闫伟红, 姜超, 等. 雀麦属野生牧草资源的搜集与评价[J]. 中国野生植物资源, 2015, 34(5): 41-45.
	MA Yubao, YAN Weihong, JIANG Chao, et al. Resources collection and evaluation of wild Bromus forage[J]. Chinese Wild Plant Resources, 2015, 34(5): 41-45.
[20]	宫珂, 靳瑰丽, 隋晓青, 等. 我国无芒雀麦种质资源分布、育种及利用现状分析[J]. 黑龙江畜牧兽医, 2019,(21): 29-32, 36.
	GONG Ke, JIN Guili, SUI Xiaoqing, et al. Analysis on the distribution, breeding and utilization of Bromus inermis germplasm resources in China[J]. Heilongjiang Animal Science and Veterinary Medicine, 2019,(21): 29-32, 36.
[21]	He Z Q, He C X, Zhang Z B, et al. Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress[J]. Colloids and Surfaces B: Biointerfaces, 2007, 59(2): 128-133.
[22]	柳洁, 肖斌, 王丽霞, 等. 丛枝菌根真菌对茶树耐盐性的影响[J]. 西北农林科技大学学报(自然科学版), 2014, 42(3): 220-225, 234.
	LIU Jie, XIAO Bin, WANG Lixia, et al. Influence of AMF on salt tolerance of tea[J]. Journal of Northwest A & F University (Natural Science Edition), 2014, 42(3): 220-225, 234.
[23]	冯希环, 刘维信, 李敏. 盐胁迫下丛枝菌根真菌对生菜生长和生理特性的影响[J]. 青岛农业大学学报(自然科学版), 2016, 33(4): 242-246.
	FENG Xihuan, LIU Weixin, LI Min. Effects of arbuscular mycorrhizal fungi on growth and physiological indices of lettuce under salt stress[J]. Journal of Qingdao Agricultural University (Natural Science), 2016, 33(4): 242-246.
[24]	王娜, 陈飞, 岳英男, 等. 松嫩盐碱草地2种优势丛枝菌根真菌对紫花苜蓿耐盐性的影响[J]. 江苏农业科学, 2017, 45(24): 146-148.
	WANG Na, CHEN Fei, YUE Yingnan, et al. Effects of two dominant arbuscular mycorrhizal fungi on salt tolerance of alfalfa in Songnen saline-alkali grassland[J]. Jiangsu Agricultural Sciences, 2017, 45(24): 146-148.
[25]	王英男, 陶爽, 华晓雨, 等. 盐碱胁迫下AM真菌对羊草生长及生理代谢的影响[J]. 生态学报, 2018, 38(6): 2187-2194.
	WANG Yingnan, TAO Shuang, HUA Xiaoyu, et al. Effects of arbuscular mycorrhizal fungi on the growth and physiological metabolism of Leymus chinensis under salt-alkali stress[J]. Acta Ecologica Sinica, 2018, 38(6): 2187-2194.
[26]	陆爽, 郭欢, 王绍明, 等. 盐胁迫下AM真菌对紫花苜蓿生长及生理特征的影响[J]. 水土保持学报, 2011, 25(2): 227-231.
	LU Shuang, GUO Huan, WANG Shaoming, et al. Effects of AM fungi on growth and physiological characters of Medicago sativa L. under NaCl stress[J]. Journal of Soil and Water Conservation, 2011, 25(2): 227-231.
[27]	周晓莹. 菌根真菌对盐胁迫下黑松幼苗生长及生理指标的影响[D]. 泰安: 山东农业大学, 2019.
	ZHOU Xiaoying. Effects of mycorrhizal fungi on growth and physiological indexes of Pinus thunbergii seedlings under salt stress[D]. Taian: Shandong Agricultural University, 2019.
[28]	陈强, 刘微, 徐小兵, 等. 4个蓝莓品种果实发育期叶片矿质营养动态及其相关性[J]. 经济林研究, 2020, 38(1): 184-189.
	CHEN Qiang, LIU Wei, XU Xiaobing, et al. Dynamics of mineral nutrients in leaves of four Vaccinium spp. cultivars during fruit development period and their correlations[J]. Non-wood Forest Research, 2020, 38(1): 184-189.
[29]	Bartels D, Sunkar R. Drought and salt tolerance in plants[J]. Critical Reviews in Plant Sciences, 2005, 24(1): 23-58.
[30]	杨海霞, 李士美, 郭绍霞. 丛枝菌根真菌对紫薇耐盐性的影响[J]. 植物生理学报, 2014, 50(9): 1379-1386.
	YANG Haixia, LI Shimei, GUO Shaoxia. Effects of arbuscular mycorrhizal fungi on salinity tolerance of Lagerstroemia indica[J]. Plant Physiology Journal, 2014, 50(9): 1379-1386.
[31]	Demiral T, Turkan I. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance[J]. Environmental and Experimental Botany, 2005, 53(3): 247-257.
[32]	梁芳, 黄秋伟, 於艳萍, 等. 濒危半红树植物玉蕊对盐胁迫的生理响应及其相关性分析[J]. 中南林业科技大学学报, 2019, 39(10): 12-18.
	LIANG Fang, HUANG Qiuwei, YU Yanping, et al. Physiological response of endangered semi-mangrove Barringtonia racemosa to salt stress and its correlation analysis[J]. Journal of Central South University of Forestry & Technology, 2019, 39(10): 12-18.
[33]	张勇, 韩多红, 晋玲, 等. 不同盐碱胁迫对红芪种子萌发和幼苗生理特性的影响[J]. 中国中药杂志, 2012, 37(20): 3036-3040.
	ZHANG Yong, HAN Duohong, JIN Ling, et al. Effects of different salt-alkaline stress on seed germination and physiological characteristics of Hedysarum polybotrys[J]. China Journal of Chinese Materia Medica, 2012, 37(20): 3036-3040.
[34]	祝文婷, 陈为京, 陈建爱, 等. 丛枝菌根真菌提高植物抗盐碱胁迫能力的研究进展[J]. 安徽农业科学, 2013, 41(5): 2061-2062, 2221.
	ZHU Wenting, CHEN Weijing, CHEN Jian’ai, et al. Research progress of arbuscular mycorrhizal fungi improving plant salt tolerance ability[J]. Journal of Anhui Agricultural Sciences, 2013, 41(5): 2061-2062, 2221.
[35]	张秋芳. 盐胁迫对盐生植物叶片SOD及光合特性的效应[D]. 济南: 山东师范大学, 2002.
	ZHANG Qiufang. Effects of salt stress on SOD and photosynthetic characteristics of halophyte leaves[D]. Jinan: Shandong Normal University, 2002.