甜瓜抗霜霉病KASP分子标记的开发与验证

doi:10.6048/j.issn.1001-4330.2024.11.003

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (11): 2626-2634.DOI: 10.6048/j.issn.1001-4330.2024.11.003

• 种质资源·分子遗传学·生理生化·微生物 • 上一篇下一篇

甜瓜抗霜霉病KASP分子标记的开发与验证

沈悦¹(), 凌悦铭², 段晓宇², 杨文莉², 李寐华², 王懿柔², 王惠林¹(), 张学军²()

1.新疆农业大学园艺学院,乌鲁木齐 830052
2.新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091

收稿日期:2024-04-19 出版日期:2024-11-20 发布日期:2025-01-08
通信作者: 王惠林(1965-),男,河南人,副教授,硕士生导师,研究方向为西甜瓜栽培与育种,(E-mail)wanghuilin@126.com；
张学军(1980-),男,吉林四平人,研究员,硕士生导师,研究方向为西瓜甜瓜抗病育种,(E-mail)zxj333@126.com
作者简介:沈悦(1999-),女,新疆人,硕士研究生,研究方向为甜瓜霜霉病抗性基因精细定位,(E-mail)shenyue052442@163.com
基金资助:
新疆维吾尔自治区天山创新团队(2022D14015);国家自然科学基金项目(32060689);财政部和农业农村部“国家现代农业产业技术体系资助”项目(CARS-25);海南省院士创新平台科研专项(吴明珠院士团队创新中心);海南省院士创新平台科研(YSPTZX202141);新疆维吾尔自治区重点研发任务专项(2022B02002)

Development and verification of KASP marker for resistance to downy mildew in muskmelon

SHEN Yue¹(), LING Yueming², DUAN Xiaoyu², YANG Wenli², LI Meihua², WANG Yirou², WANG Huilin¹(), ZHANG Xuejun²()

1. College of Horticulture,Xinjiang Agricultural University, Urumqi 830052, China
2. Hami Melon Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2024-04-19 Published:2024-11-20 Online:2025-01-08
Supported by:
Tianshan Innovation Team of Xinjiang Uygur Autonomous Region(2022D14015);Project of National Natural Science Foundation of China(32060689);MOF and MARA“China Agriculture Research System”(CARS-25);Hainan Provincial Academician Innovation Platform Scientific Research Special Project (Academician WU Mingzhu Team Innovation Center);Hainan Provincial Academician Innovation Platform Scientific Research Special Project(YSPTZX202141);Xinjiang Uygur Autonomous Region Key Research and Development Program for Special Projects(2022B02002)

摘要/Abstract

摘要：

【目的】开发及验证与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,为甜瓜抗霜霉病分子标记辅助育种提供理论基础。【方法】以高抗霜霉病甜瓜品种“PI 438685”与高感霜霉病甜瓜品种“黄旦子”为亲本,构建F₂代分离群体。利用BSA-seq技术,对甜瓜霜霉病抗性基因进行QTL定位,根据SNP分布位置在定位区间内开发66对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,并使用F₂代分离群体进行筛选验证。【结果】霜霉病抗性基因定位到9号染色体末端区域,筛选出9对在双亲间具有多态性的KASP分子标记。获得1对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记(KASP_24832572),其基因分型准确率为79%,准确度较高。【结论】在9号染色体上定位到1个与甜瓜霜霉病抗性相关的区间,在此区间内开发并筛选出1对与甜瓜霜霉病抗性基因紧密连锁的KASP分子标记,该标记有助于甜瓜抗霜霉病分子标记辅助育种。

关键词: 甜瓜; 霜霉病; BSA-seq; KASP分子标记; 分子标记辅助育种

Abstract:

【Objective】 This study aims to develop and validate KASP molecular markers closely linked to melon downy mildew resistance genes, in the hope of providing a theoretical basis for downy mildew resistance molecular marker assisted breeding of downy mildew resistance in melons.【Methods】 The accession ‘PI 438685’ with high resistance of downy mildew, and the accession ‘Huangdanzi’ with high sensitivity of downy mildew were used as parents to construct a separate population.QTL mapping of downy mildew resistance genes in melon was drawn through a combination of BSA and whole genome sequencing.KASP molecular markers closely linked to downy mildew resistance genes were developed within this interval, and then screened and validated using F₂separate populations.【Results】 The downy mildew resistance gene was located on chromosome 9 through BSA analysis.We developed 66 pair of KASP molecular markers in the candidate region, screened 9 pairs of KASP molecular markers with high specificity.Subsequently, the 9 pairs of KASP molecular markers were validated, the KASP molecular marker (KASP-24832572), closely linked to downy mildew resistance genes was obtained, and its accuracy of distinguish genotype could reach 79%.【Conclusion】 One QTL related to downy mildew resistance has been located on chromosome 9 by BSA analysis, and a pair of KASP molecular markers closely linked to downy mildew resistance genes are developed in this interval, which has contributed to downy mildew resistance breeding in melons.

Key words: melon; downy mildew; BSA-seq; KASP molecular markers; molecular markers assisted breeding

中图分类号:

S436.5

沈悦, 凌悦铭, 段晓宇, 杨文莉, 李寐华, 王懿柔, 王惠林, 张学军. 甜瓜抗霜霉病KASP分子标记的开发与验证[J]. 新疆农业科学, 2024, 61(11): 2626-2634.

SHEN Yue, LING Yueming, DUAN Xiaoyu, YANG Wenli, LI Meihua, WANG Yirou, WANG Huilin, ZHANG Xuejun. Development and verification of KASP marker for resistance to downy mildew in muskmelon[J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2626-2634.

图/表 8

参考文献 33

[1]	Tabara M, Nagashima Y, He K, et al. Frequent asymptomatic infection with tobacco ringspot virus on melon fruit[J]. Virus Research, 2021, 293: 198266.
[2]	Thomas A, Carbone I, Lebeda A, et al. Virulence structure within populations of Pseudoperonospora cubensis in the United States[J]. Phytopathology, 2017, 107(6): 777-785. DOI PMID
[3]	李晨光. 黄瓜、甜瓜霜霉病流行学初步研究[D]. 长春: 吉林农业大学, 2008.
	LI Chenguang. Primary Epidemicological Studies of Cucumber and Muskmelon Downy Milew[D]. Changchun: Jilin Agricultural University, 2008.
[4]	Hamza A M, Essa T A, Derbalah A S, et al. Performance of Some Fungicide Alternatives for Controlling Powdery Mildew on Cucumber under Greenhouse Conditions[J]. Egyptian journal of pest control, 2015, 25(3):647-654.
[5]	Wallace E C, D’Arcangelo K N, Quesada-Ocampo L M. Population analyses reveal two host-adapted clades of Pseudoperonospora cubensis, the causal agent of cucurbit downy mildew, on commercial and wild cucurbits[J]. Phytopathology, 2020, 110(9): 1578-1587. DOI PMID
[6]	张猛. 防治甜瓜霜霉病药剂筛选试验[J]. 新农业, 2019,(1): 39-40.
	ZHANG Meng. Screening test of chemicals for controlling melon downy mildew[J]. New Agriculture, 2019,(1): 39-40.
[7]	Innark P, Panyanitikoon H, Khanobdee C, et al. QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers[J]. Journal of Genetics, 2020, 99: 81.
[8]	Wallace E C, Quesada-Ocampo L M. Analysis of microsatellites from the transcriptome of downy mildew pathogens and their application for characterization of Pseudoperonospora populations[J]. PeerJ, 2017, 5: e3266.
[9]	张学军, 杨俊涛, 李寐华, 等. 甜瓜PI414723抗霜霉病基因SSR分子标记[J]. 华北农学报, 2016, 31(S1): 86-91. DOI
	ZHANG Xuejun, YANG Juntao, LI Meihua, et al. SSR molecular marker of downy mildew resistance gene of melon PI414723[J]. Acta Agriculturae Boreali-Sinica, 2016, 31(S1): 86-91.
[10]	贺玉花, 徐永阳, 徐志红, 等. 甜瓜霜霉病抗性基因的SSR标记[J]. 江苏农业科学, 2014, 42(7): 54-55.
	HE Yuhua, XU Yongyang, XU Zhihong, et al. SSR markers of resistance genes to downy mildew in melon[J]. Jiangsu Agricultural Sciences, 2014, 42(7): 54-55.
[11]	李丽, 王海岗, 张晓丽, 等. SSR分子标记在作物遗传育种中的应用[J]. 山西农业科学, 2008, 36(3): 15-18.
	LI Li, WANG Haigang, ZHANG Xiaoli, et al. SSR marker and its application to plant genetics and breeding[J]. Journal of Shanxi Agricultural Sciences, 2008, 36(3): 15-18.
[12]	Grover A, Sharma P C. Development and use of molecular markers: past and present[J]. Critical Reviews in Biotechnology, 2016, 36(2): 290-302. DOI PMID
[13]	Semagn K, Babu R, Hearne S, et al. Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement[J]. Molecular Breeding, 2014, 33(1): 1-14.
[14]	Yang X Q, Li Y, Zhang W W, et al. Fine mapping of the uniform immature fruit color gene u in cucumber (Cucumis sativus L.)[J]. Euphytica, 2014, 196(3): 341-348.
[15]	Cheng Z K, Liu Z G, Xu Y C, et al. Fine mapping and identification of the candidate gene BFS for fruit shape in wax gourd (Benincasa hispida)[J]. Theoretical and Applied Genetics, 2021, 134(12): 3983-3995.
[16]	许勇, 张春秋, 宫国义, 等. 用于鉴定甜瓜果肉颜色的高通量分子标记及其专用引物: CN105200144A[P].
	XU Yong, ZHANG Chunqiu, GONG Guoyi, et al. High throughput molecular markers and specific primers for identifying the color of melon flesh: CN 201510691195[P].
[17]	Branham S E, Daley J, Levi A, et al. QTL mapping and marker development for tolerance to sulfur phytotoxicity in melon (Cucumis melo)[J]. Frontiers in Plant Science, 2020, 11: 1097. DOI PMID
[18]	Branham S E, Kousik C, Mandal M K, et al. Quantitative trait loci mapping of resistance to powdery mildew race 1 in a recombinant inbred line population of melon[J]. Plant Disease, 2021, 105(12): 3809-3815.
[19]	Cao Y Y, Diao Q N, Chen Y Y, et al. Development of KASP markers and identification of a QTL underlying powdery mildew resistance in melon (Cucumis melo L.) by bulked segregant analysis and RNA-seq[J]. Frontiers in Plant Science, 2021, 11:593207.
[20]	Criswell A D, Wehner T C, Klosinska U, et al. Use of sporulation and other leaf and vine traits for evaluation of resistance to downy mildew in cucumber1[J]. Research Gate, 2008..
[21]	Épinat C, Pitrat M. Inheritance of resistance to downy mildew (Pseudoperonospora cubensis) in muskmelon (Cucumis melo).I.Analysis of a 8 x 8 diallel table[J]. Agronomie, 1994, 14(4): 239-248.
[22]	Thomas A, Carbone I, Choe K, et al. Resurgence of cucurbit downy mildew in the United States: Insights from comparative genomic analysis of Pseudoperonospora cubensis[J]. Ecology and Evolution, 2017, 7(16): 6231-6246. DOI PMID
[23]	Wang Kexin, Li Shumin, Meng Fanjun, et al. The occurrence patterns and comprehensive control methods of melon downy mildew in Jilin Province[J]. Agricultural Development and Equipment, 2017 (12): 1.
[24]	Zhang S P, Liu M M, Miao H, et al. Chromosomal mapping and QTL analysis of resistance to downy mildew in Cucumis sativus[J]. Plant Disease, 2013, 97(2): 245-251. DOI PMID
[25]	衣杰, 李晓红, 刘晓红, 等. 利用芽孢杆菌防治甜瓜霜霉病试验研究[J]. 河南农业科学, 2004,(2): 44-46. DOI
	YI Jie, LI Xiaohong, LIU Xiaohong, et al. Studies on the effect of Bacillus on muskmelon downy mildew[J]. Journal of Henan Agricultural Sciences, 2004,(2): 44-46.
[26]	Epinat C, Pitrat M, Bertrand F. Genetic analysis of resistance of five melon lines to powdery mildews[J]. Euphytica, 1992, 65(2): 135-144.
[27]	Thomas C E. Inheritance of resistance to downy mildew in Cucumis melo[J]. Plant Disease, 1988, 72(1): 33.
[28]	Ivanoff S S. Resistance of cantaloupes to downy mildew and the melon aphid[J]. Journal of Heredity, 1944, 35(2): 35-39.
[29]	MORE T A, SESHADRI V S. Studies on genetic divergence inmuskmelon (Cucumis melo L.)[J]. Journal of Maharashtra Agricultur-al Universities, 2002, 27(2),121-137.
[30]	Hayward A C, Tollenaere R, Dalton-Morgan J, et al. Molecular marker applications in plants[J]. Methods in Molecular Biology, 2015, 1245: 13-27. DOI PMID
[31]	Perchepied L, Dogimont C, Pitrat M. Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f.sp.melonis race 1.2 in a recombinant inbred line population of melon[J]. TAG Theoretical and Applied Genetics Theoretische Und Angewandte Genetik, 2005, 111(1): 65-74.
[32]	张学军, 宁雪飞, 杨永, 等. 甜瓜PI₃90452霜霉病抗性基因的QTL定位[J]. 新疆农业科学, 2016, 53(12): 2157-2165.
	ZHANG Xuejun, NING Xuefei, YANG Yong, et al. QTL Mapping of Resistance Genes to Downy Mildew in Cucumis melo ssp.melo PI₃90452[J]. Xinjiang Agricultural Sciences, 2016, 53(12): 2157-2165.
[33]	Toporek S M, Branham S E, Katawczik M L, et al. QTL mapping of resistance to Pseudoperonospora cubensis clade 1, mating type A2, in Cucumis melo[J]. TAG Theoretical and Applied Genetics Theoretische Und Angewandte Genetik, 2021, 134(8): 2577-2586.

病情级别 Disease level	病情分级标准 Disease grading standards
0	无任何症状
1	病斑面积占整个叶片面积的1/10以下
2	病斑面积占整个叶片面积的1/10 ~ 1/4
3	病斑面积占整个叶片面积的1/4 ~ 2/4
4	病斑面积占整个叶片面积的2/4 ~ 3/4
5	病斑面积占整个叶片面积的3/4 以上或者叶片干枯

病情级别 Disease level	病情分级标准 Disease grading standards
0	无任何症状
1	病斑面积占整个叶片面积的1/10以下
2	病斑面积占整个叶片面积的1/10 ~ 1/4
3	病斑面积占整个叶片面积的1/4 ~ 2/4
4	病斑面积占整个叶片面积的2/4 ~ 3/4
5	病斑面积占整个叶片面积的3/4 以上或者叶片干枯

样品 Sample	原始数据量 Raw Base(bp)	有效数据量 Clean Base(bp)	Q20(%)	Q30(%)	GC总含量 GC Content(%)	比对率 Mapping rate(%)
huangdanzi	5 300 455 200	5 166 262 500	96.4	90.7	36.81	94.32
PI 438685	5 010 953 100	4 957 239 900	96.81	91.45	37.14	93.18
3-0	10 016 053 800	9 928 662 900	97.41	93.02	36.88	95.66
3-5	9 512 647 800	9 396 773 400	97.12	92.44	37.45	95.86

样品 Sample	原始数据量 Raw Base(bp)	有效数据量 Clean Base(bp)	Q20(%)	Q30(%)	GC总含量 GC Content(%)	比对率 Mapping rate(%)
huangdanzi	5 300 455 200	5 166 262 500	96.4	90.7	36.81	94.32
PI 438685	5 010 953 100	4 957 239 900	96.81	91.45	37.14	93.18
3-0	10 016 053 800	9 928 662 900	97.41	93.02	36.88	95.66
3-5	9 512 647 800	9 396 773 400	97.12	92.44	37.45	95.86

引物名称 Primer name	序列(5’-3’) Sequence (5’-3’)			是否具有特异性 Is it polymorphic
KASP_23055782	GAAGGTGACCAAGTTCATGCTAAATTATTCAAGTTGCCAAACTCCTTGATA			是
KASP_23063365	GAAGGTGACCAAGTTCATGCTCTTAGACAAAACTCTCCCACCTC			否
KASP_23067310	GAAGGTGACCAAGTTCATGCTGAAAATTTTGGGGAGATTGAGAAGAGA			是
KASP_23070054	GAAGGTGACCAAGTTCATGCTATTTCCTTAGTTTTACAACGGACCATG			是
KASP_23071460	GAAGGTGACCAAGTTCATGCTGGAAAGGATCTTTTAATATACTTTAGACCT			是
KASP_23080398	GAAGGTGACCAAGTTCATGCTAATAAAGAAAAGAAAGAGGAAGAGGATTGA			是
KASP_23092852	GAAGGTGACCAAGTTCATGCTAGTGTTTTGTGACAAACATCATACCGA			否
KASP_23093210	GAAGGTGACCAAGTTCATGCTGGCAACATAGTCCTACTCTAACTCA			是
KASP_23094651	GAAGGTGACCAAGTTCATGCTACAATTGGGGTAATAAGGAAGTGGA			否
KASP_23112033	GAAGGTGACCAAGTTCATGCTCACACACACACACGGTTTAGAGAA			是
KASP_23120772	GAAGGTGACCAAGTTCATGCTCATCGATACCAAATTTGTAAGCAATCATAAT			是
KASP_23247274	GAAGGTGACCAAGTTCATGCTGACGAGCAAAATCCAGGGTGG			是
KASP_23299420	GAAGGTGACCAAGTTCATGCTTTGTCTACCTCTTCTCCTCT			否
KASP_23308722	GAAGGTGACCAAGTTCATGCTCATGGAAACGTGATCAATGAAGCGA			是
KASP_23399433	GAAGGTGACCAAGTTCATGCTGTGTTCATTATGCTTTAAGGCTATTGC			是
KASP_23399886	GAAGGTGACCAAGTTCATGCTGAAAGTTCTATGGTTCTCTGAGCC			是
KASP_23401162	GAAGGTGACCAAGTTCATGCTGGTAGAAATGCTTAACAATCATCTTCCA			是
KASP_23401360	GAAGGTGACCAAGTTCATGCTAAGCACAGCAAAGAGTTCAGATTCC			否
KASP_23402938	GAAGGTGACCAAGTTCATGCTGCAATGCAATTCCTTAAAGGTGCTG			否
KASP_23404381	GAAGGTGACCAAGTTCATGCTATGTCTTCCGCTTTTATGAGTTGAAAC			否
KASP_23406473	GAAGGTGACCAAGTTCATGCTAATTTAATTTCACTTCCCTTTTATTTCACCAT			是
KASP_23408092	GAAGGTGACCAAGTTCATGCTATTTAGTACCTTCTAAAGATGGTGCATTAT			是
KASP_23420662	GAAGGTGACCAAGTTCATGCTATGAATCTACTTCGCATAAGTTCCATC			是
KASP_23470437	GAAGGTGACCAAGTTCATGCTCGAAGAAATCTGCACCGTCCTG			是
KASP_23470767	GAAGGTGACCAAGTTCATGCTTTGCCTACGTTTGTATGGAAGCTG			否
KASP_23470860	GAAGGTGACCAAGTTCATGCTCGAAGCTATTCTCTGGCAACTCA			否
KASP_23473896	GAAGGTGACCAAGTTCATGCTATTTCATGGGAAATGGAAGTCAAATGC			是
KASP_23476195	GAAGGTGACCAAGTTCATGCTAACCTGCAACCCATTTCAACATTGTA			是
KASP_23517447	GAAGGTGACCAAGTTCATGCTCCAAAAATAGGTCCTTGTTAGTGCAC			否
KASP_23518588	GAAGGTGACCAAGTTCATGCTCAAATTTCAATTTAAGTGATTCTAGCAAATAC			否
KASP_23518973	GAAGGTGACCAAGTTCATGCTGACATTGCGACTACTACCAATGGTT			否
KASP_23526537	GAAGGTGACCAAGTTCATGCTGGAGGCAAGAACTCCTAGACACA			否
KASP_23527871	GAAGGTGACCAAGTTCATGCTTAGACTTCTCAAAACCAACGGCA			否
KASP_23530097	GAAGGTGACCAAGTTCATGCTCGTGAATGTGAAAAAGAAAGACAAAAAAG			否
KASP_23530714	GAAGGTGACCAAGTTCATGCTACCAACTTTTAGAGACACTTCACGC			是
KASP_23554586	GAAGGTGACCAAGTTCATGCTAGACTAGTTGAATTATGCTCGAGTTCA			是
KASP_23592188	GAAGGTGACCAAGTTCATGCTTTAATGTGATGAACAACAACCTCCAC			否
KASP_23623803	GAAGGTGACCAAGTTCATGCTAGAAGTCTAAGCAACACATACAGAG			否
KASP_23630472	GAAGGTGACCAAGTTCATGCTGTTTGTTGAAATAGACACCAAAGGAAG			是
KASP_23693029	GAAGGTGACCAAGTTCATGCTGAACTTTGGAATTGTAATTGCTTGCTTAATT			否
KASP_23757315	GAAGGTGACCAAGTTCATGCTAGGTGAGGAATAAGATGTGCTAGC			是
KASP_23774894	GAAGGTGACCAAGTTCATGCTCAGGACTCTGTAATTAGCAAGAGAAC			否
KASP_23779883	GAAGGTGACCAAGTTCATGCTGGTGGGGAAGTGGGTTAGGC			否
KASP_23789334		GAAGGTGACCAAGTTCATGCTAAACGCTCCCCTAATAAATAGACCC	否
KASP_23789363		GAAGGTGACCAAGTTCATGCTTGTAGACAGGTAGACAAGATTCAACA	是
KASP_23789561		GAAGGTGACCAAGTTCATGCTTCACCCCTTGGAGTCATTGGG	是
KASP_23794137		GAAGGTGACCAAGTTCATGCTATCTTTCAGTGTTCAAAAGGACTGGAA	否
KASP_23796506		GAAGGTGACCAAGTTCATGCTCTCCTTAACCTTTTTCCCAAATTTTCT	是
KASP_23797202		GAAGGTGACCAAGTTCATGCTCTCTTTTATGATGTTCGTTCTCTA	否
KASP_23797386		GAAGGTGACCAAGTTCATGCTCCTCGCAATGAGTCAAAACAGATG	否
KASP_23797740		GAAGGTGACCAAGTTCATGCTGGAAAGGAAGATAACGATTCAGATAAGTT	是
KASP_23798623		GAAGGTGACCAAGTTCATGCTAAGCCTAGACATATTGGTACAATAACG	是
KASP_23799658		GAAGGTGACCAAGTTCATGCTAACGATAATGCAGAAGATCATGTTGAC	是
KASP_23855524		GAAGGTGACCAAGTTCATGCTTAGAAGAGAATTAAATGTTGGCGGCA	否
KASP_23891190		GAAGGTGACCAAGTTCATGCTAAATGAATAAAATACTCCACGTTTAGAGG	否
KASP_23898936		GAAGGTGACCAAGTTCATGCTAAATGTGCTCTAAATATGTTTCTTCACATG	否
KASP_23903385		GAAGGTGACCAAGTTCATGCTAAATTGACGAGTTTTTGATCATTTTATGGG	否
KASP_23907245		GAAGGTGACCAAGTTCATGCTAAGAAAATGGCGAGGAAGAAAGAGAAA	否
KASP_23934209		GAAGGTGACCAAGTTCATGCTCATACCCTCTATAGCCACAAGTACAA	是
KASP_23940394		GAAGGTGACCAAGTTCATGCTAAGAAAAGAAATGAATGGACACAC	是
KASP_23999679		GAAGGTGACCAAGTTCATGCTCAAATTTTTGTGTATAGGAACTGCGC	否
KASP_24032929		GAAGGTGACCAAGTTCATGCTATGCATTTCTCTTTCATATGTAATCTCTTG	否
KASP_24060858		GAAGGTGACCAAGTTCATGCTGTTCAGCCCAAAAGTCCATCAAGTA	是
KASP_24065711		GAAGGTGACCAAGTTCATGCTCTCCTTGATGTTCTTTCTCACCA	否
KASP_24066737		GAAGGTGACCAAGTTCATGCTCATGTTATGAATGTGGAGGGAGCT	否
KASP_24832572		GAAGGTGACCAAGTTCATGCT CTCCACTGGTTTAGGAGGAAGA	是

甜瓜抗霜霉病KASP分子标记的开发与验证

Development and verification of KASP marker for resistance to downy mildew in muskmelon

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[1]	张浩, 梁其干, 张学军, 符小发, 陈积豪, 周勃, 黄远. 刺角瓜砧木抗性分析及其嫁接对甜瓜品质的影响[J]. 新疆农业科学, 2024, 61(8): 1963-1968.
[2]	陈积豪, 张浩, 梁其干, 符小发, 张学军, 毛建才. 减施化肥增施蚯蚓粪有机肥对连作甜瓜的影响[J]. 新疆农业科学, 2024, 61(8): 1969-1975.
[3]	卡地尔阿依·买买提, 周婷婷, 韩盛, 梅丽克汗·热西提, 玉山江·麦麦提. 不同甜瓜品种遗传转化再生体系的建立与基因编辑植株的快速获取[J]. 新疆农业科学, 2024, 61(7): 1666-1672.
[4]	杨君妍, 闫淼, 吴海波, 杨文莉, 王豪杰, 毛建才, 翟文强, 李俊华. 高温对不同厚皮甜瓜品种种子萌发的影响及其耐热性综合评价[J]. 新疆农业科学, 2024, 61(6): 1386-1396.
[5]	刘阳, 张郑啸, 白羽嘉, 冯作山. 链格孢菌侵染对甜瓜不同组织活性氧代谢的影响[J]. 新疆农业科学, 2024, 61(6): 1397-1406.
[6]	马凌, 沈琦, 康琪, 张忠祥, 贾宏涛, 王成. 甜瓜不同生长阶段5种重金属在植株中富集分配的变化及关联分析[J]. 新疆农业科学, 2024, 61(4): 892-899.
[7]	杨文莉, 许丽蓉, 刘斌, 凌悦铭, 李寐华, 杨永, 范蓉, 黎玉顺, 张永兵, 张学军. 盐胁迫对薄皮甜瓜‘灰鼠’离子平衡、膜脂过氧化及渗透调节物质积累的影响[J]. 新疆农业科学, 2024, 61(4): 900-907.
[8]	梁其干, 张浩, 胡国智, 陈积豪, 冯烔鑫, 曹庆, 王敏, 符小发, 闫淼, 高强, 张学军, 周勃, 王豪杰. 施肥调控对设施甜瓜生长与产量、品质的影响[J]. 新疆农业科学, 2024, 61(3): 599-606.
[9]	李超, 杨英, 郑贺云, 杨建丽, 陈伟, 杨咪, 孙玉萍. 基于SSR荧光标记分析新疆甜瓜种质资源遗传多样性与群体结构[J]. 新疆农业科学, 2024, 61(11): 2614-2625.
[10]	姚军, 秦勇, 郑贺云, 张翠环, 再吐娜·买买提, 汪志伟, 耿新丽. 无花果叶提取液复合保鲜膜的研制及其在甜瓜保鲜上的应用[J]. 新疆农业科学, 2024, 61(1): 118-126.
[11]	张立国, 郭春贵, 常立春, 郭新磊, 张涛, 武剑, 梁建丽, 高杰, 王晓武. 白菜抗根肿病分子标记开发与种质资源鉴定[J]. 新疆农业科学, 2023, 60(9): 2281-2290.
[12]	郑贺云, 姚军, 李超, 张翠环, 耿新丽. 不同贮藏温度对甜瓜品质的影响及预测模型建立[J]. 新疆农业科学, 2023, 60(8): 1950-1957.
[13]	姚军, 郑贺云, 张翠环, 再吐娜·买买提, 耿新丽. LED持续光照处理对甜瓜贮藏特性及糖代谢的影响[J]. 新疆农业科学, 2023, 60(7): 1689-1697.
[14]	李自芹, 李文绮, 贾文婷, 李宇辉, 刘成江. 氯化钙与1-MCP对西州蜜甜瓜采后贮藏品质的影响[J]. 新疆农业科学, 2023, 60(7): 1698-1704.
[15]	刘阳, 白羽嘉, 张郑啸, 楚晨俐, 王甜甜, 冯作山. 甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化[J]. 新疆农业科学, 2023, 60(7): 1780-1789.