甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化

doi:10.6048/j.issn.1001-4330.2023.07.026

摘要/Abstract

摘要：

【目的】分析常温贮藏期间(25℃)不同甜瓜果皮与果肉组织病程相关蛋白和苯丙烷代谢相关酶的差异,探究甜瓜在常温贮藏下的内源抗病性。【方法】甜瓜果实损伤接种链格孢,贮藏期间分离甜瓜果实外果皮、内果皮和果肉组织,测定不同贮藏天数时甜瓜不同组织的病程相关蛋白和苯丙烷代谢相关酶,分析其不同组织抗病能力的差异性,分析不同贮藏温度对甜瓜抗病性的影响。【结果】贮藏期间伽师瓜的病斑直径小于86-1甜瓜,染菌后16 d,86-1甜瓜的果皮和果肉病斑分别是伽师瓜的1.55倍和1.38倍;在此期间甜瓜内几丁质酶(Chitinase,CHT)、β-1,3-葡聚糖酶(β-1,3-Glucan,GLU)、甜瓜4-香豆酰辅酶A连接酶(Coumaroyl-CoA,4CL)、苯丙氨酸解氨酶(L-Phenylalanin Ammo-Nialyase,PAL)、肉桂酸-4-羟化酶(Cinnamic Acid 4-Hydroxylase,C4H)活性呈先升高后下降趋势,伽师瓜酶活性高于86-1甜瓜,两种甜瓜的酶活性均为外果皮>内果皮>果肉组织,伽师瓜比86-1甜瓜更快到达酶活性高峰。【结论】损伤接种链格孢温度对甜瓜果实病程相关蛋白和苯丙烷代谢酶的变化趋势无明显影响,酶活性均呈现先升高后下降的趋势;伽师瓜优于86-1甜瓜,果皮抗病能力强于果肉,伽师瓜比86-1甜瓜更积极抵御链格孢的侵染。

关键词: 链格孢菌; 甜瓜; 病程相关蛋白; 苯丙烷代谢

Abstract:

【Objective】 To analyze the differences of related proteins and enzymes involved in phenylpropanoid metabolism in pathogenesis from diverse melons’ peel and pulp tissues, which are stored in room temperature (25℃) and explore the endogenous disease resistance of melons stored at room temperature.【Methods】 Melon was were inoculated with Alternaria alternata, and their epricarp, endocarp and pulp tissues were separated during the storage. The pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in different tissues of muskmelons were measured in different storage days,and the disease resistance of different tissues of melon were analyzed. The indicators in these issues were measured every other day to deduce their the disease resistance. Compared with previous researches, the effects of different storage temperatures on disease resistance of muskmelon were figured out as well.【Results】 During storage, the lesion diameter of Jiashigua was smaller than 86-1 melon’s, the lesion size in the peel and pulp of 86-1 melons reached 1.55 and 1.375 times bigger than Jiashigua’s on the 16th day. Activities of Chitinase (CHT) and β-1,3-glucanase (GLU) in melon showed a trend of increasing first and then decreasing, and the enzyme activity of Jiashigua was higher than 86-1 melon’s, exocarp was higher than endocarp and pulp tissue. Compared to 86-1 melon, Jiashigua reached the peak of enzyme activity in a shorter time.【Conclusion】 It is found that temperature has no obvious effect on the change trend of the pathogenesis related proteins and enzymes involved in phenylpropanoid metabolism in melons, and enzyme activities all show a trend of increasing at first and then decreasing; In terms of disease resistance, Jiashigua is superior to 86-1 melon. In dealing with the infection of Alternaria alternata, peels have stronger disease resistance than pulps do, so Jiashigua is more active to resist than 86-1 melon facing the infection of Alternaria alternata.

Key words: Alternaria alternata; muskmelon; pathogenesis related proteins; phenylpropanoid metabolism

刘阳, 白羽嘉, 张郑啸, 楚晨俐, 王甜甜, 冯作山. 甜瓜抗链格孢侵染病程相关蛋白及苯丙烷代谢酶的变化[J]. 新疆农业科学, 2023, 60(7): 1780-1789.

LIU Yang, BAI Yujia, ZHANG Zhengxiao, CHU Chenli, WANG Tiantian, FENG Zuoshan. Changes of related proteins and phenylpropane metabolic enzymes in melon infected by Alternaria alternata[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1780-1789.

图/表 17

图1 链格孢侵染后甜瓜病斑直径的变化

Fig.1 Change in lesion diameter in two melon by A. alternaria

图2 不同天数甜瓜病斑注:a(伽师瓜6 d)、b(86-1甜瓜6 d)、c(伽师瓜10 d)、d(86-1甜瓜10 d)、e(伽师瓜16 d)、f(86-1甜瓜16 d)

Fig.2 Change in disease spot size of two melon in different days Note:a (Jiashigua 6 d), b(86-1 melon 6 d), c(Jiashigua 10 d), d(86-1 melon 10 d), e(Jiashigua 16 d), f(86-1 melon 16 d)

图3 链格孢侵染后甜瓜外果皮CHT变化

Fig.3 Changes in CHT activity in melon epricarp infected by A. alternaria

图4 链格孢侵染后甜瓜内果皮CHT变化

Fig.4 Changes in CHT activity in melon endocarp by A. alternaria

图5 链格孢侵染后甜瓜果肉CHT变化

Fig.5 Changes in CHT activity in melon pulp by A. alternaria

图6 链格孢侵染后甜瓜外果皮GLU变化

Fig.6 Changes in GLU activity in melon epricarp by A. alternaria

图7 链格孢侵染后甜瓜内果皮GLU变化

Fig.7 Changes in GLU activity in melon endocarp by A. alternaria

图8 链格孢侵染后甜瓜果肉GLU变化

Fig.8 Changes in GLU activity in melon pulp by A. alternaria

图9 链格孢侵染后甜瓜外果皮PAL变化

Fig.9 Changes in PAL activity in melon by A. alternaria pericarp

图10 链格孢侵染后甜瓜内果皮PAL变化

Fig.10 Changes in PAL activity in melon by A. alternaria endocarp

图11 链格孢侵染后甜瓜果肉PAL变化

Fig.11 Changes in PAL activity in melon pulp by A. alternaria

图12 链格孢侵染后甜瓜外果皮C4H变化

Fig.12 Changes in C4H activity in melon epricarp A. alternaria

图13 链格孢侵染后甜瓜内果皮C4H变化

Fig.13 Changes in C4H activity in melon endocarp A. alternaria

图14 链格孢侵染后甜瓜果肉C4H变化

Fig.14 Changes in C4H activity in melon pulp A. alternaria

图15 链格孢侵染后甜瓜外果皮4CL变化

Fig.15 Changes in 4CL activity in melon epricarp A. alternaria

图16 链格孢侵染后甜瓜内果皮4CL变化

Fig.16 Changes in 4CL activity in melon endocarp A. alternaria

图17 链格孢侵染后甜瓜果肉4CL变化

Fig.17 Changes in 4CL activity in melon pulp A. alternaria

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