基于加工用番茄果实及果肉组织主要性状的相关性分析

doi:10.6048/j.issn.1001-4330.2019.05.002

摘要/Abstract

摘要： 【目的】研究不同加工用番茄种质资源果肉外果皮细胞厚度和离体耐放性等性状间的相关性,为加工用番茄品种选育的田间耐贮运性改良奠定基础。【方法】比较12份加工用番茄高代纯合种质资源的果实主要性状,测定参试材料果实横切面情况,结合石蜡切片技术比较分析各试材的果肉组织外果皮厚度,研究不同材料自然室温放置35 d后的果实重量损失和腐烂率,并分析参试材料各调查性状间的相关性。【结果】主要果实性状差异显著,果肉组织外果皮厚度在6.75~10.30 μm变化,平均外果皮厚度为8.311 μm,12份材料的果实横切面面积存在一定的差异,常温放置35 d后的腐烂率在11.67%~98.33%变化,平均腐烂率为55.50%,失重率在17.36%~63.68%变化,平均失重率为31.96%,各调查性状间的相关系数介于-0.903^**~0.875^**,均呈不同程度的相关性,常温放置35 d后的腐烂率与果肉组织外果皮厚度呈显著的负相关。【结论】外果皮越厚果实常温放置若干天后的腐烂率越低,对于选育优异的田间耐放性好的加工用番茄品种有重要参考。

关键词: 加工番茄; 常温; 耐贮; 腐烂率; 相关性

Abstract: 【Objective】 The aim of his paper is to study the correlation and cluster of different characters in different pepper resources and lay a foundation for the further improvement of Xinjiang dry pepper varieties. 【Method】The 24 pepper advanced self-line germplasm resources were employed in this paper to evaluate the main fruit's traits, the main characteristic of the pulp cells structure and the time-consuming of the fruit losing 75% water. Thereafter, the correlation and cluster analysis was implemented to all the characteristic. By comparing the main characteristics of 12 processed tomato high-purity germplasm resources, the cross section of the fruit of the test material was determined. The thickness of the outer fruit skin of each test piece was compared and analyzed by the paraffin section technique. 【Result】The results showed that the positive and negative significance correlation existed among the characteristic, and the correlation coefficient ranged from -0.625^** to 0.976^**. Furthermore, 24 pepper germplasm resources could be divided into five groups by euclidean distance UPGMA method. The first groups included 8 lines, the second group included six lines, the third group included three lines, the fourth group included six lines, and the fifth group only included one line which implied the peculiarity of this line. 【Conclusion】The thicker the exocarp, the lower the decay rate of the fruit after being placed at room temperature for several days. This has important reference value for breeding excellent tomato varieties with good field tolerance

Key words: processing tomato; normal temperature; resistance to storage; rotting rate; correlation

杨涛, 唐亚萍, 张国儒, 李晓琴, 帕提古丽, 王柏柯, 李宁, 王娟, 余庆辉, 杨生保. 基于加工用番茄果实及果肉组织主要性状的相关性分析[J]. 新疆农业科学, 2019, 56(5): 797-807.

YANG Tao, TANG Ya-ping, ZANG Guo-ru, LI Xiao-qin, Patiguli, WANG Bai-ke, LI Ning, WANG Juan, YU Qing-hui, YANG Sheng-bao. Correlation Analysis of the Processing Tomato Based on the Main Characteristics of the Fruit and Pulp Tissue[J]. Xinjiang Agricultural Sciences, 2019, 56(5): 797-807.

参考文献 43

[1]	Bergougnoux, Véronique. The history of tomato: From domestication to biopharming [J]. Biotechnology Advances, 2014, 32(1):170-189.
[2]	余庆辉, 赵得提, 韩启新, 等. 加工番茄生产技术创新战略研究[J]. 新疆农业科学, 2006, 43(S1): 1-4.YU Qing-hui, ZHAO De-ti, HAN Qi-xin, et al. Strategic Study on Innovative Producing Technology of Processing Tomato[J]. Xinjiang Agricultural Sciences, 2006, 43(S1): 1-4. (in Chinese)
[3]	杨生保, 杨涛, 李宁, 等. 番茄ILs果实性状的主成分分析与聚类分析[J]. 植物遗传资源学报, 2015, 16(4): 907-913.YANG Sheng-bao, YANG Tao, LI Ning, et al. (2015). Principal Component and Cluster Analysis Based on Fruit Traits in ILs of Tomato [J]. Journal of Plant Genetic Resources, 2015,16(4): 907-913. (in Chinese)
[4]	杨生保, 唐亚萍, 杨涛, 等. 加工型番茄果实硬度特异材料的果实特性及果肉组织特征[J]. 农业工程学报, 2017, 33(18): 285-290.YANG Sheng-bao, TANG Ya-ping, YANG Tao, et al.(2017). Fruit characteristic and flesh tissue feature of special firmness type processing tomato cultivar [J]. Transactions of the Chinese Society of Agricultural Engineering, 33(18): 285-290. (in Chinese)
[5]	傅力, 刘彤, 徐立生, 等. 番茄酱中几种微生物指标检测方法的探讨[J]. 新疆农业大学学报, 2002, 25(4): 51-53.FU Li, LIU Tong, XU Li-sheng, et al. (2002).Studies on Examination Methods of Several Microbiological Indexes of Tomato Paste [J]. Journal of Xinjiang Agricultural University, 25(4): 51-53. (in Chinese)
[6]	杨涛, 杨生保, 唐亚萍, 等. 番茄果实耐压QTL遗传效应初步分析[J]. 园艺学报, 2017, 44(7): 1 371-1 378.YANG Tao, YANG Sheng-Bao, TANG Ya-Ping, et al. (2017).Genetic Effect Analysis of QTLs Conferring Fruit Firmness in Tomato[J]. Acta Horticulturae Sinica, 44(7): 1,371-1,378. (in Chinese)
[7]	刘磊, 宋燕, 郑峥, 等. 利用Solanum pennellii LA0716渐渗系群体初步定位番茄果实硬度QTL[J], 植物遗传资源学报, 2015, 16(2): 323-329.LIU Lei, SONG Yan, ZHENG Zheng, et al. (2015). QTL Mapping of Fruit Firmness with an Introgression Line Population Derived from the Wild Tomato Species Solanum pennellii LA0716 [J]. Journal of Plant Genetic Resources, 16(2): 323-329.(in Chinese)
[8]	Chapman N H, Bonnet J, Grivet L, et al. (2012).High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus[J]. Plant Physiology, 159(4):1,644-1,657.
[9]	Yang S , Yu Q , Wang B , et al. (2016).Identification of QTLs for red fruit firmness using the wild tomato species＼r, Solanum pennellii＼r, LA716 introgression lines[J]. Plant Breeding, 135(6): 728-734.
[10]	Uluisik S , Chapman N H , Smith R , et al. (2016).Genetic improvement of tomato by targeted control of fruit softening[J]. Nature Biotechnology, 34(10): 950-952.
[11]	Yu Q H, Wang B, Li N, et al. (2017).CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines[J]. Scientific Reports, 7(1):11,874.
[12]	曹玉鑫, 曹红霞, 王萍, 等. 营养液浓度对番茄生长、品质以及耐贮性的影响[J]. 食品科学, 2018, 39(7): 63-70.CAO Yu-xin, CAO Hong-xia, WANG Ping, et al. (2018).Effects of Nutrient Solution Concentration on Growth,Quality and Storability of Tomato[J]. Food Science, 39(7): 63-70. (in Chinese)
[13]	董啊娜, 汪炳良, 郑积荣, 等. 无限生长型番茄果实耐贮藏性研究[J]. 浙江农业学报, 2009, 21(4): 350-353.DONG A-na, WANG Bing-liang, ZHENG Ji-rong, et al. (2009).Fruit storage characteristics of various infinite-growth tomato cultivars [J]. Acta Agriculturae Zhejiangensis, 21(4): 350-353. (in Chinese)
[14]	张学杰, 王金玉, 郭科, 等. 不同加工番茄品种的加工特性评价[J]. 食品科学, 2009, 30(15): 33-35.ZHANG Xue-jie, WANG Jin-yu, GUO Ke, et al. (2009). Evaluation on Tomato Cultivars Suitable for Canned, Concentrate, and Juice Processing [J]. Food Science, 30(15): 33-35. (in Chinese)
[15]	王丽娟, 宋思圆, 姜鹏, 等. 不同去皮方法对番茄去皮效果和品质的影响[J]. 食品科学, 2017, 38(5): 26-31.WANG Li-Juan, SONG Si-Yuan, JIANG Peng, et al. (2017). Effect of Different Peeling Methods on the Peeling Efficiency and Quality of Tomatoes [J]. Food Science, 38(5): 26-31. (in Chinese)
[16]	Hiwasa-Tanase, K., & Ezura, H. (2014).Fruit Ripening: 1 Climacteric and Non-climacteric Ripening [M]. New Delhi: CAB International, 1-14.
[17]	Vrebalov, J., Ruezinsky, D. , Padmanabhan, V, White, R. , Medrano, D., & Drake, R., et al. (2002).A MADS-Box gene necessary for fruit ripening at the tomato ripening-inhibitor (Rin) locus[J]. Science, 296(5566): 343-346.
[18]	Martel, C., Vrebalov, J., Tafelmeyer, P. , & Giovannoni, J. J. (2001). The tomato MADS-Box transcription factor RIPENING INHIBITOR interacts with promoters involved in numerous ripening processes in a COLORLESS NONRIPENING-Dependent manner [J]. Plant Physiology, 157(3): 1,568-1,579.
[19]	Li L , Zhu B , Fu D, et al. (2011). RIN transcription factor plays an important role in ethylene biosynthesis of tomato fruit ripening[J]. Journal of the Science of Food & Agriculture, 91(13): 2,308-2,314.
[20]	Li L, Zhu B , Yang P, et al.( 2011).The regulation mode of RIN transcription factor involved in ethylene biosynthesis in tomato fruit[J]. Journal of the Science of Food & Agriculture, 91(10): 1,822-1,828.
[21]	Mattoo A K, Vickery R S. (1977).Subcellular Distributions of Isoenzymes in Fruits of a Normal Cultivar of Tomato and of the RIN Mutant at Two Stages of Development[J]. Plant Physiology, 60(4): 496-498.
[22]	Kumar R , Sharma M K , Kapoor S , et al. (2012). Transcriptome analysis ofrinmutant fruit and in silico analysis of promoters of differentially regulated genes provides insight into LeMADS-RIN-regulated ethylene-dependent as well as ethylene-independent aspects of ripening in tomato[J]. Molecular Genetics & Genomics Mgg, 287(3):189-203.
[23]	Fujisawa M , Nakano T , Shima Y , et al. (2013).A large-scale identification of direct targets of the tomato MADS box transcription factor RIPENING INHIBITOR reveals the regulation of fruit ripening [J]. Plant Cell, 25(2):371-386.
[24]	Gao C, Ju Z, Cao D, et al. (2015).MicroRNA profiling analysis throughout tomato fruit development and ripening reveals potential regulatory role of RIN on microRNAs accumulation[J]. Plant Biotechnology Journal, 13(3):370-382.
[25]	Kitagawa M , Nakamura N , Usuda H , et al. (2006).Ethylene Biosynthesis Regulation in Tomato Fruit from the F1 Hybrid of the ripening inhibitor (rin) Mutant[J]. Journal of the Agricultural Chemical Society of Japan, 70(7): 1,769-1,772.
[26]	Kitagawa M , Moriyama T , Ito H , et al. (2006).Reduction of Allergenic Proteins by the Effect of the ripening inhibitor (rin) Mutant Gene in an F1 Hybrid of the rin Mutant Tomato[J]. Journal of the Agricultural Chemical Society of Japan, 70(5): 1,227-1,233.
[27]	Kalenahalli, Y. N., & Ramanjini, G. P. H. (2013). Phenotypic and molecular characterization of a tomato (Solanum lycopersicum L.) F-2 population segregation for improving shelf life[J]. Genetics and Molecular Research, 12(1): 506-518.
[28]	Thompson, A. J . (1999).Molecular and Genetic Characterization of a Novel Pleiotropic Tomato-Ripening Mutant [J]. PLANT PHYSIOLOGY, 120(2):383-390.
[29]	Fraser P D, Bramley P, Seymour G B. (2001).Effect of the Cnr mutation on carotenoid formation during tomato fruit ripening.[J]. Phytochemistry, 58(1):75-79.
[30]	Orfila C , Huisman M M H , Willats W G T , et al. (2002). Altered cell wall disassembly during ripening of Cnr tomato fruit: implications for cell adhesion and fruit softening[J]. Planta, 215(3):440-447.
[31]	Manning, K., T R, M., Poole, M, et al. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening [J]. Nature Genetics, 38(8): 948-952.
[32]	Klee, H. J., & Giovannoni, J. J. (2011).Genetics and control of tomato fruit ripening and quality attributes[J]. Annual Review of Genetics, 45(1): 41-59.
[33]	Lanahan, M. B., Yen, H. C., Giovannoni, J. J., & Klee, H. J. (1994).The never ripe mutation blocks ethylene perception in tomato[J]. The Plant Cell, 6(4): 521-530.
[34]	Saladie, M., Matas, A. J., Isaacson, T. , Jenks, M. A., Goodwin S. M., & Niklas K.J., et al. (2007).A reevaluation of the key factors that influence tomato fruit softening and integrity [J]. Plant Physiology, 144(2): 1,012-1,028.
[35]	Lytovchenko, A. , Beleggia, R., Schauer, N. , Isaacson, T. , Fernie, A. R. , & Hellmann, H. , et al.(2009).Application of GC-MS for the detection of lipophilic compounds in diverse plant tissues[J]. Plant Methods, 5(1): 1-11.
[36]	王立浩,张正海,曹亚从, 等. "十二五"我国辣椒遗传育种研究进展及其展望[J]. 中国蔬菜, 2016, (1): 1-7.WANG Li-Hao, ZHANG Zheng-Hai, Cao Ya-Cong, et al. (2016).Progress and prospect of pepper genetic breeding in China during the 12th five-year plan [J]. China Vegetables, (1): 1-7. (in Chinese)
[37]	芮文婧, 王晓敏, 张倩男, 等. 番茄353份种质资源表型性状遗传多样性分析[J]. 园艺学报, 2018, 45(3): 561-570.RUI Wen-jing, WANG Xiao-min, ZHANG Qian-nan, et al. (2018). Genetic Diversity Analysis of 353 Tomato Germplasm Resources by Phenotypic Traits [J]. Acta Horticulturae Sinica, 45(3): 561-570. (in Chinese)
[38]	刘希艳, 郑峥, 邓学斌, 等. 中国加工番茄资源遗传多样性分析, 园艺学报, 2016, 43(3): 485-495.LIU Xi-yan, ZHENG Zhen, DENG Xue-bin, et al. (2016).Analysis the Genetic Diversity of Processing Tomato in China [J]. Acta Horticulturae Sinica, 43(3): 485-495. (in Chinese)
[39]	Yeats, T. H., Buda, G. J., Wang, Z. H., Chehanovsky, N. , Moyle, L. C., & Jetter, R., et al. (2012).The fruit cuticles of wild tomato species exhibit architectural and chemical diversity, providing a new model for studying the evolution of cuticle funct(in Chinese)ion[J]. Plant Journal, 69(4): 655-666.
[40]	Fernandez-Moreno, J. P., Levy-Samoha, D., Malitsky, S., Monforte, A. J., Orzaez, D., & Aharoni, A., et al.( 2017).Uncovering quantitative trait loci and candidate genes for tomato fruit cuticular lipid composition using the solanum pennellii introgression line population [J]. Journal of Experimental Botany, 68(11): 2,703-2,716.
[41]	李三培, 华德平, 高星, 等. 不同类型甜瓜成熟过程中果肉质地及其细胞显微结构的变化[J]. 西北植物学报, 2017, 37(6): 1 118-1 125.LI San-pei, HUA De-ping., GAO Xing, et al.( 2017). Variation Characteristics of Flesh Texture and Cell Microstructure of Different Types of Melon during Ripening [J]. Acta Botanica Boreali-Occidentalia Sinica, 37(6): 1,118-1,125. (in Chinese)
[42]	卢纹岱, 朱红兵. SPSS统计分析(第5版) [M]. 北京:电子工业出版社, 2015.LU Wen-dai, ZHU Hong-bing. (2015). SPSS statistical analysis (the 5th Ed.) [M]. Beijing: Electronic Industry Press. (in Chinese)
[43]	Kissinger, M., Tuvia-Alkalai, S., Shalom, Y., Fallik, E., Fallik, E., & Jenks, M. A., et al. (2005).Characterization of physiological and biochemical factors associated with postharvest water loss in ripe pepper fruit during storage [J]. Journal of the American Society for Horticultural Science, 130(5): 735-741

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