Abstract:

【Objective】 Optimization of SE medium of a saline-alkali green algae,the purpose of this study is to provide a theoretical basis for the research on the molecular biology and ecological environment protection of saline-alkali green alga as well as fishery bait resources.【Methods】 A saline-alkali green alga isolated and purified from East Lake of Tarim University is screened for culture medium, morphological observation and molecular identification.Investigate the effects of CaCl₂·2H₂O, KH₂PO₄, NaCl, NaNO₃, MgSO₄·7H₂O and pH on its growth, and conduct phylogenetic analysis.【Results】 The morphological characteristics showed that the algae is ellipse.The optimum concentration of CaCl₂·2H₂O is 0.050 g/L, the soluble sugar is 24.34%, the soluble protein is 16.55%, and the total chlorophyll is 2.000 g/L.The optimum concentration of KH₂PO₄ is 0.175 g/L, the soluble sugar is 10.53%, the soluble protein is 7.7%, and the total chlorophyll is 3.607 g/L.The optimum concentration of NaCl is 0.100 g/L, the soluble sugar is 11.82%, the soluble protein is 8.57%, and the total chlorophyll is 3.385 g/L.The optimum concentration of NaNO₃ is 0.250 g/L, the soluble sugar is 10.64%, the soluble protein is 6.10%, and the total chlorophyll is 2.731 g/L.The optimum concentration of MgSO₄·7H₂O is 0.375 g/L, the soluble sugar is 12.32%, the soluble protein is 9.9%, and the total chlorophyll is 1.790 g/L.The optimum pH concentration is 9.0, the soluble sugar is 16.57%, the soluble protein is 9.46%, and the total chlorophyll is 1.985 g/L.The results showed that the sequence of No.1 algae is closely related to the novel Pseudochlorococcum sp.【Conclusion】 This study determined the classification status of the saline No.1 and the concentration of CaCl₂·2H₂O, KH₂PO₄, NaCl and NaNO₃ after SE medium optimization was 0.050 g/L, 0.175 g/L, 0.100 g/L, 0.250 g/L, respectively. The concentration of MgSO₄·7H₂O is 0.375 g/L and the pH is 9.0.

Key words: saline-alkali green alga; 18S rDNA; morphological properties; medium optimization

摘要：

【目的】 优化一株盐碱绿藻的SE培养基,为盐碱藻分子生物学资源的研究提供理论依据。【方法】 对塔里木大学校园内东湖中分离纯化的一株盐碱绿藻进行培养基筛选,通过形态观察及分子鉴定,研究CaCl₂·2H₂O、KH₂PO₄、NaCl、NaNO₃、MgSO₄·7H₂O以及pH对其生长的影响,并进行系统发育分析。【结果】 盐碱绿藻为椭圆形藻。CaCl₂·2H₂O最适浓度为0.050 g/L,可溶性糖为24.34%,可溶性蛋白为16.55%,总叶绿素为2.000 g/L。KH₂PO₄最适浓度为0.175 g/L,可溶性糖为10.53%,可溶性蛋白为7.7%,总叶绿素为3.607 g/L。NaCl最适浓度为0.100 g/L,可溶性糖为11.82%,可溶性蛋白为8.57%,总叶绿素为3.385 g/L。NaNO₃最适浓度为0.250 g/L,可溶性糖为10.64%,可溶性蛋白为6.10%,总叶绿素为2.731g/L。MgSO₄·7H₂O最适浓度为0.375 g/L,可溶性糖为12.32%,可溶性蛋白为9.9%,总叶绿素为1.790 g/L。pH最适浓度为9.0,可溶性糖为16.57%,可溶性蛋白为9.46%,总叶绿素为1.985 g/L。1号藻的序列与新颖拟绿球藻属(Pseudochlorococcum sp.)聚簇成一支亲缘关系很相近。【结论】 确定了1号盐碱绿藻的分类地位以及SE培养基优化后CaCl₂·2H₂O的浓度为0.050 g/L,KH₂PO₄的浓度为0.175 g/L,NaCl的浓度为0.100 g/L,NaNO₃的浓度为0.250 g/L,MgSO₄·7H₂O的浓度为0.375 g/L和pH为9.0。

关键词: 盐碱绿藻, 18S rDNA, 形态特性, 培养基优化