Abstract: 【Objective】 The project aims to optimize the fermentation media of antagonistic bacteria JK1 against jujube black rot by response surface methodology in the hope of improving the concentration of spores in flask culture conditions.【Method】After determining the carbon and nitrogen source of the medium by single factor experiment, the most important factors affecting the fermentation of JK1 in the fermentation medium were analyzed by the Plackett Burman test and then the steepest slope method was used to test the main factors and the optimum range of the main factors was obtained. Finally, the optimal level of the main factors was ascertained by response surface analysis.【Result】The optimal formulation of fermentation medium was: malt extract 25.00 g/L, glucose 0.57 g/L, soya peptone 12.5 g/L, NaCl 2.7 g/L, K₂HPO₄ 0.25 g/L and MgSO₄ 0.62g/L.【Conclusion】JK1 was cultured by the optimized fermentation medium, and the count of spore increased 3.4 times and reached 4.92 × 10⁹ CFU/mL., which had provided data for the further application of Bacillus polymyxides.

Key words: jujube black rot; Paenibacillus polymyxa; spore production; fermentation medium optimization; response surface test

摘要： 【目的】 采用响应面法对红枣黑斑病的拮抗细菌JK1培养基进行优化,提高其发酵产物中芽孢的浓度。为该菌应用提供数据。【方法】单因素实验确定培养基碳氮源物质,Plackett-Burman试验分析发酵培养基中对JK1发酵影响最重要的主要因素;运用最陡爬坡法对主要因素进行试验,获得主要因素的最适范围。通过响应面分析得到主要因素的最优水平。【结果】优化后的培养基组成为:麦芽浸粉 25.00 g/L、葡萄糖 0.57 g/L、大豆蛋白胨 12.5 g/L、NaCl 2.7 g/L、K₂HPO₄ 0.25 g/L 、MgSO₄ 0.625 g/L。【结论】在最优发酵培养基培养下,多粘类芽孢杆菌的芽孢数提高了3.4倍,达到4.92×10⁹CFU/mL。

关键词: 红枣黑斑病, 多粘类芽孢杆菌, 芽孢产量, 培养基优化,响应面试验