Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (1): 171-177.DOI: 10.6048/j.issn.1001-4330.2023.01.019
• Plant Protection · Soil Fertilizer · Prataculture · Animal Husbandry Veterinarian • Previous Articles Next Articles
LI Chunyan(), LIU Fangting, ZHANG Wangbin()
Received:
2022-05-13
Online:
2023-01-20
Published:
2023-03-07
Correspondence author:
ZHANG Wangbin(1974-), Male, from Chengcheng, Shaanxi Province, associate professor, master's degree, majoring in plant pathology, (E-mail)zwbzky@163.com
Supported by:
通讯作者:
张王斌(1974-),男,陕西澄城人,教授,硕士,硕士生导师,研究方向为植物病理学,(E-mail)zwbzky@163.com
作者简介:
李春艳(1992-),女,河南驻马店人,硕士研究生,研究方向为植物病理学,(E-mail)387863197@qq.com
基金资助:
CLC Number:
LI Chunyan, LIU Fangting, ZHANG Wangbin. Identification of Pathogens Causing the Abnormal Appearance of Apples in Alar Based on High-Throughput Sequencing Technology[J]. Xinjiang Agricultural Sciences, 2023, 60(1): 171-177.
李春艳, 刘芳婷, 张王斌. 基于高通量测序对引起苹果外观异常病原的鉴定[J]. 新疆农业科学, 2023, 60(1): 171-177.
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URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2023.01.019
编号 Number | 寄主 Host | 品种 Variety | 采集部位 To collect parts | 症状表现 Symptoms |
---|---|---|---|---|
DZFS-1 | 苹果 | 短枝富士 | 果实 | 果实花脸 |
WL-1 | 苹果 | 王林 | 果实 | 果实畸形 |
XSJ-1 | 苹果 | 新世纪 | 果实 | 果实裂果 |
DZFS-2 | 苹果 | 短枝富士 | 枝条 | 无明显症状 |
WL-2 | 苹果 | 王林 | 枝条 | 无明显症状 |
XSJ-2 | 苹果 | 新世纪 | 枝条 | 无明显症状 |
Table 1 Basic sample information
编号 Number | 寄主 Host | 品种 Variety | 采集部位 To collect parts | 症状表现 Symptoms |
---|---|---|---|---|
DZFS-1 | 苹果 | 短枝富士 | 果实 | 果实花脸 |
WL-1 | 苹果 | 王林 | 果实 | 果实畸形 |
XSJ-1 | 苹果 | 新世纪 | 果实 | 果实裂果 |
DZFS-2 | 苹果 | 短枝富士 | 枝条 | 无明显症状 |
WL-2 | 苹果 | 王林 | 枝条 | 无明显症状 |
XSJ-2 | 苹果 | 新世纪 | 枝条 | 无明显症状 |
名称 Name | 面积 Area (667m2) | 果园 个数 Number of orchard | 果园品种 Variety |
---|---|---|---|
西一垄 West One ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西二垄 West Second ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西三垄 West three ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西四垄 West Fourth ridge | 200 | 5 | 短枝富士、王林、新世纪 |
Table 2 Basic information of the survey site
名称 Name | 面积 Area (667m2) | 果园 个数 Number of orchard | 果园品种 Variety |
---|---|---|---|
西一垄 West One ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西二垄 West Second ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西三垄 West three ridge | 200 | 5 | 短枝富士、王林、新世纪 |
西四垄 West Fourth ridge | 200 | 5 | 短枝富士、王林、新世纪 |
分离株 Isolate | GenBank 登录号 GenBank accession | 来源 Source | 全长 Overall length(nt) |
---|---|---|---|
AgrCV | HE963831 | 加拿大 | 9 266 |
AgrCV | LC476839 | 韩国 | 5 573 |
AgrCV | KT835289 | 意大利 | 9 266 |
ASGV | KJ579253 | 中国 | 6 495 |
ASGV | MN131071 | 美国 | 5 738 |
ASGV | LC475148 | 韩国 | 6 480 |
ASGV | MK929792 | 巴西 | 6 475 |
ASGV | MK599422 | 中国 | 6 496 |
ASGV | KX668488 | 巴西 | 6 495 |
ASGV | LT160740 | 印度 | 6 458 |
ASGV | KV947036 | 中国 | 6 509 |
ASGV | JQ308181 | 中国 | 6 495 |
ASPV | AB045371 | 日本 | 9 293 |
ASPV | KF319056 | 德国 | 9 284 |
ASPV | KU308398 | 中国 | 9 270 |
ASPV | FR694186 | 印度 | 9 267 |
ACLSV | M58152 | - | 7 555 |
ACLSV | AJ243438 | 德国 | 7 545 |
ACLSV | AB326224 | 日本 | 7 553 |
ACLSV | MK929794 | 巴西 | 7 511 |
ACLSV | MK929796 | 巴西 | 7 521 |
ApMV | AF174584 | 美国 | 3 476 |
ApMV | HE574162 | 印度 | 3 476 |
ApMV | KY883318 | 澳大利亚 | 3 435 |
ApMV | KY965060 | 加拿大 | 3 328 |
ApMV | KY965059 | 加拿大 | 3 477 |
Table 3 GenBank has logged into the isolates of the full length apple genome
分离株 Isolate | GenBank 登录号 GenBank accession | 来源 Source | 全长 Overall length(nt) |
---|---|---|---|
AgrCV | HE963831 | 加拿大 | 9 266 |
AgrCV | LC476839 | 韩国 | 5 573 |
AgrCV | KT835289 | 意大利 | 9 266 |
ASGV | KJ579253 | 中国 | 6 495 |
ASGV | MN131071 | 美国 | 5 738 |
ASGV | LC475148 | 韩国 | 6 480 |
ASGV | MK929792 | 巴西 | 6 475 |
ASGV | MK599422 | 中国 | 6 496 |
ASGV | KX668488 | 巴西 | 6 495 |
ASGV | LT160740 | 印度 | 6 458 |
ASGV | KV947036 | 中国 | 6 509 |
ASGV | JQ308181 | 中国 | 6 495 |
ASPV | AB045371 | 日本 | 9 293 |
ASPV | KF319056 | 德国 | 9 284 |
ASPV | KU308398 | 中国 | 9 270 |
ASPV | FR694186 | 印度 | 9 267 |
ACLSV | M58152 | - | 7 555 |
ACLSV | AJ243438 | 德国 | 7 545 |
ACLSV | AB326224 | 日本 | 7 553 |
ACLSV | MK929794 | 巴西 | 7 511 |
ACLSV | MK929796 | 巴西 | 7 521 |
ApMV | AF174584 | 美国 | 3 476 |
ApMV | HE574162 | 印度 | 3 476 |
ApMV | KY883318 | 澳大利亚 | 3 435 |
ApMV | KY965060 | 加拿大 | 3 328 |
ApMV | KY965059 | 加拿大 | 3 477 |
品种 Variety | 症状 Symptoms | 发病率 Incidence rate(%) |
---|---|---|
王林 Wang Lin | 畸形 | (74.50±5.97)a |
短枝富士 Short Branch Fuji | 着色异常、裂果 | (70.50±7.98)ab |
新世纪 New Century | 裂果 | (63.50±8.40)b |
Table 4 Comparison of the incidence of apple appearance abnormality among different varieties
品种 Variety | 症状 Symptoms | 发病率 Incidence rate(%) |
---|---|---|
王林 Wang Lin | 畸形 | (74.50±5.97)a |
短枝富士 Short Branch Fuji | 着色异常、裂果 | (70.50±7.98)ab |
新世纪 New Century | 裂果 | (63.50±8.40)b |
果园编号 Orchard number | 发病率 Incidence rate(%) |
---|---|
西一垄 West One ridge | (83.20±5.40)a |
西二垄 West Second ridge | (78.20±3.16)ab |
西三垄 West three ridge | (73.20±4.14)b |
西四垄 West Fourth ridge | (47.20±8.67)c |
Table 5 Comparison of the incidence of abnormal apple appearance in different orchards
果园编号 Orchard number | 发病率 Incidence rate(%) |
---|---|
西一垄 West One ridge | (83.20±5.40)a |
西二垄 West Second ridge | (78.20±3.16)ab |
西三垄 West three ridge | (73.20±4.14)b |
西四垄 West Fourth ridge | (47.20±8.67)c |
品种 Variety | 病毒种类(种) Virus species (species) |
---|---|
王林 Wang Lin | 苹果茎沟病毒(ASGV)、苹果褪绿叶斑病毒(ACLSV)、苹果茎痘病毒(ASPV)、苹果绿皱果病毒(AgrCV)、苹果花叶病毒(ApMV) |
短枝富士 Short Branch Fuji | 苹果绿皱果病毒(AgrCV)、苹果茎痘病毒(ASPV)、苹果茎沟病毒(ASGV) |
新世纪 New Century | 苹果茎沟病毒(ASGV)、苹果褪绿叶斑病毒(ACLSV)、苹果茎痘病毒(ASPV)、苹果绿皱果病毒(AgrCV) |
Table 6 The apple appears to carry an abnormal virus type
品种 Variety | 病毒种类(种) Virus species (species) |
---|---|
王林 Wang Lin | 苹果茎沟病毒(ASGV)、苹果褪绿叶斑病毒(ACLSV)、苹果茎痘病毒(ASPV)、苹果绿皱果病毒(AgrCV)、苹果花叶病毒(ApMV) |
短枝富士 Short Branch Fuji | 苹果绿皱果病毒(AgrCV)、苹果茎痘病毒(ASPV)、苹果茎沟病毒(ASGV) |
新世纪 New Century | 苹果茎沟病毒(ASGV)、苹果褪绿叶斑病毒(ACLSV)、苹果茎痘病毒(ASPV)、苹果绿皱果病毒(AgrCV) |
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