Effects of Supplemental Fermented Soybean Meal on Fecal Metabolites in Yili Foal Based on Metabolomics Analysis

doi:10.6048/j.issn.1001-4330.2022.11.026

Abstract

Abstract:

【Objective】 The purpose of this experiment is to study the effect of supplementing fermented soybean meal on fecal metabolites of Yili foal, so as to explore the application effect of fermented soybean meal in the research and development of special feed for Yili foal, and for the development and digestion of special feed for Yili foal. System health provides a theoretical basis.【Methods】 In this experiment, 15 12-month-old Yili foals with similar body condition and no history of digestive system diseases were selected and randomly divided into three groups with 5 horses in each group. The control group, the test group I and the test group II were supplemented with 5％ ordinary soybean meal, 2.5％ ordinary soybean meal ＋ 2.5％ fermented soybean meal and 5％ fermented soybean meal on the basis of hay and ordinary concentrated feed, respectively. The animals were pre-fed for 7 days, and the experimental period was 56 days.【Results】 A total of 9 metabolites with significant differences were screened out, and betaine, fructosylglycine, mannitol and niacin in the experimental group were significantly higher than those in the control group (P<0.05). KEGG pathway analysis, mainly enriched in niacin, glycine, serine and threonine metabolism and ABC transporters and other pathways.【Conclusion】 Supplemental feeding of fermented soybean meal can regulate the metabolism of carbohydrates, proteins, fats and amino acids in Yili foals, and has various biological functions such as antioxidant, anti-inflammatory, stress relief, and osmotic pressure regulation, which is of positive significance for horse health.

Key words: Yili foal; fermented soybean meal; metabolism of feces

摘要：

【目的】研究补喂发酵豆粕对伊犁马驹粪便代谢物的影响,分析发酵豆粕在伊犁马驹专用饲料研发中的应用效果,为伊犁马驹专用饲料研发以及消化系统健康提供理论依据。【方法】选取15匹体况相近、无消化系统疾病病史的12月龄伊犁马驹,随机分为3组,每组5匹马。对照组、试验I组和试验II组分别在干草和普通精饲料的基础上添加5％的普通豆粕、2.5％普通豆粕＋2.5％发酵豆粕和5％的发酵豆粕。预饲7 d,试验期为56 d。【结果】共筛选出9种显著性差异代谢物,试验组甜菜碱、果糖基甘氨酸、甘露醇和烟酸显著高于对照组(P<0.05)。主要富集到烟酸、甘氨酸、丝氨酸和苏氨酸代谢以及ABC转运蛋白等通路。【结论】补喂发酵豆粕能够调节伊犁马驹碳水化合物、蛋白质、脂肪和氨基酸代谢,并且具有抗氧化、抗炎、缓解应激、调节渗透压等多种生物学功能。

关键词: 伊犁马驹, 发酵豆粕, 粪便代谢物

CLC Number:

S821.9

ZHONG Haojie, YAO Xinkui, LUO Penghui, MENG Jun, YAO Yueyang, WANG Chuankun, REN Wanlu. Effects of Supplemental Fermented Soybean Meal on Fecal Metabolites in Yili Foal Based on Metabolomics Analysis[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2800-2807.

钟浩杰, 姚新奎, 罗鹏辉, 孟军, 姚岳扬, 王川坤, 任万路. 基于代谢组学分析补喂发酵豆粕对伊犁马驹粪便代谢物的影响[J]. 新疆农业科学, 2022, 59(11): 2800-2807.

Figures/Tables 10

References 22

[1]	Gibney M J, Walsh M, Brennan L, et al. Metabolomics in human nutrition: opportunities and challenges[J]. American Journal of Clinical Nutrition, 2005, 82(3): 497-503. PMID
[2]	Patti G J, Yanes O, Siuzdak G. Innovation: Metabolomics: the apogee of the omics trilogy[J]. Nature Reviews Molecular Cell Biology, 2012, 13(6): 263-269. DOI URL
[3]	Hotz C, Gibson R S. Traditional food-processing and preparation practices to enhancing the bioavailability of micronutrients in plant-based diets[J]. The Journal of Nutrition, 2007, 137:1097-1100. DOI URL
[4]	Egounlety M, Aworh O C. Effect of soaking, dehulling,cooking and fermentation with Rhizopus oligosporus on the oligosaccharides, trypsin inhibitor, phytic acid and tannins of soybean (Glycine max Merr.), cowpea (Vigna unguiculata L.Walp) and ground bean (Macrotyloma geocarpa Harms)[J]. Journal of Food Engineering, 2003, 56:249-254. DOI URL
[5]	Frias J Y, Song S, Martínez-Villaluenga C, et al. Immunoreactivity and amino acid content of fermented soybean products[J]. Journal of Agricultural and Food Chemistry, 2008, 56:99-105. PMID
[6]	Wang Y, Luwq, Lidf, et al. Energy and ilea digestible amino acid concentrations for growing pigs and performance of weanling pigs fed fermented or conventional soybean meal[J]. Asian-Australasian Journal of Animal Sciences, 2014, 27(5): 706-716.
[7]	Espinosa C D, Oliveira M S F, Lagos L V, et al. Nutritional value of a new source of fermented soybean meal fed to growing pigs[J]. Journal of Animal Science, 2020, 98(12): 357. DOI URL
[8]	吴丹丹, 滕乐邦, 栾正庆, 等. 小肽对奶牛瘤胃微生物蛋白产量、产奶性能和氮排泄的影响[J]. 动物营养学报, 2016, 28(4): 1019-1098.
	WU Dandan, TENG Lebang, LUAN Zhengqing, et al. Effects of small peptides on rumen microbial protein yield, milk performance and nitrogen excretion of dairy cows[J]. Journal of Animal Nutrition, 2016, 28(4): 1019-1098.
[9]	Feng J, Liu X, Xu Z R, et al. Effects of fermented soybean meal on digestive enzyme activities and intestinal morphology in broilers[J]. Poultry Science, 2007, 86(6): 1149-1154. PMID
[10]	Escalona EE, Leng J, Dona A C, et al. Dominant components of the thoroughbred metabolome characterised by 1H-nuclear magnetic resonance spectroscopy: a metabolite atlas of common biofluids[J]. Equine Veterinary Journal, 2015, 47:721-730. DOI PMID
[11]	Anderson JR, Phelan MM, Clegg PD, et al. Synovial fluid metabolites differentiate between septic and nonseptic joint pathologies[J]. Proteome Research, 2018, 17:2735-2743. DOI URL
[12]	Beachler T, Gracz H, Long NM, et al. Allantoic metabolites, progesterone, and estradiol-17β remain unchanged after infection in an experimental model of equine ascending placentitis[J]. Journal of Equine Veterinary Science, 2019, 73:95-105. DOI
[13]	Bazzano M, Laghi L, Zhu C, et al. Metabolomics of tracheal wash samples and exhaled breath condensates in healthy horses and horses affected by equine asthma[J]. Journal of Breath Research, 2018, 12(3):046015. DOI URL
[14]	郭雪峰, 边连全, 付亮亮. 甜菜碱在畜牧业上的应用研究[J]. 今日畜牧兽医, 2007, 2(3): 49-50.
	GUO Xuefeng, BIAN Lianquan, FU Liangliang, et al. Application of betaine in animal husbandry[J]. Today's Animal Husbandry and Veterinary, 2007, 2(3): 49-50.
[15]	岳萌萌, 卜也, 何丽华, 等. 甜菜碱的生物学功能及在肉牛生产中的应用研究[J]. 黑龙江畜牧兽医, 2021, 6(11): 44-47.
	YUE Mengmeng, BU Ye, HE Lihua, et al. Biological function of betaine and its application in beef cattle production[J]. Heilongjiang Animal Husbandry and Veterinary, 2021, 6(11): 44-47.
[16]	Craig S A. Betaine in human nutrition[J]. The American Journal of Clinical Nutrition, 2004, 3(80): 539-549.
[17]	Gao X, Zhang H, Guo X F, et al. Effect of Betaine on Reducing Body Fat-A Systematic Review and Meta-Analysis of Randomized Controlled Trials[J]. Nutrients, 2019, 11(10): 2480. DOI URL
[18]	贺绍君, 赵书景, 李静, 等. 甜菜碱对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响[J]. 动物营养学报, 2014, 26(12): 3731-3739.
	HE Shaojun, ZHAO Shujuan, LI Jing, et al. Effects of betaine on growth performance, duodenal digestive enzyme activity and cecal microflora in Heat Stressed Broilers[J]. Journal of Animal Nutrition, 2014, 26(12): 3731-3739.
[19]	龙凡, 梅文亮, 许兰娇, 等. 烟酸的生物学功能及其在畜禽生产中的应用[J]. 动物营养学报, 2014, 26(12): 3731-3739.
	LONG Fan, MEI Wenliang, XU Lanqiao, et al. Biological function of niacin and its application in livestock and poultry production[J]. Journal of Animal Nutrition, 2014, 26(12): 3731-3739.
[20]	Kim H S, Xiao C Y, Wang R H, et al. Hepatic-specific disruption of SIRT6 in mice results in fatty liver formation due to enhanced glycolysis and triglyceride synthesis[J]. Cell Metabolism, 2010, 3(12): 224-236.
[21]	Ding H Y, Li Y, Liu L H, et al. Sirtuin 1 is involved in oleic acid-induced calf hepatocyte steatosis via alterations in lipid metabolim-related proteins[J]. Journal of Animal Science, 2021, 10(99): 250.
[22]	Kamanna V S, Ganji S H, Kashyap M L. Recent advances in niacin and lipid metabolism[J]. Current Opinion in Lipidology, 2013, 3(24):239-245.

项目 Items		对照组 Control group	试验组 Experimental group
原料 Ingredients (％)	玉米	25.0	25.0
	豆粕	5.00	0.00
	发酵豆粕	0.00	5.00
	燕麦	26.00	26.00
	大麦	33.0	33.0
	清油	3.00	3.00
	糖蜜	3.00	3.00
	预混料¹	5.00	5.00
	合计	100.00	100.00
营养水平 Nutrient levels	消化能DE/(MJ/kg)	14.62	14.00
	粗蛋白质	10.96	11.21
	粗脂肪	6.29	6.19
	钙	0.49	0.50
	磷	0.26	0.27

项目 Items		对照组 Control group	试验组 Experimental group
原料 Ingredients (％)	玉米	25.0	25.0
	豆粕	5.00	0.00
	发酵豆粕	0.00	5.00
	燕麦	26.00	26.00
	大麦	33.0	33.0
	清油	3.00	3.00
	糖蜜	3.00	3.00
	预混料¹	5.00	5.00
	合计	100.00	100.00
营养水平 Nutrient levels	消化能DE/(MJ/kg)	14.62	14.00
	粗蛋白质	10.96	11.21
	粗脂肪	6.29	6.19
	钙	0.49	0.50
	磷	0.26	0.27

电离模式 Ionization	项目 Items	组别 GroupB-A
POS	R²Y Q²	0.931 0.479
NEG	R²Y Q²	0.936 0.472

电离模式 Ionization	项目 Items	组别 GroupB-A
POS	R²Y Q²	0.931 0.479
NEG	R²Y Q²	0.936 0.472

代谢物名称 Metabolite name	变量投影重要度 VIP	P值 P-Value	趋势 Trent
高香草酸Homovanillic acid	1.74	<0.01	↓
丙酮酸Pyruvic acid	1.83	<0.02	↓
果糖基甘氨酸Fructosylglycine	1.36	<0.03	↑
甜菜碱Betaine	1.70	<0.02	↑
甘露醇Mannitol	1.68	<0.03	↑
苯乙醇Phenylethyl alcohol	1.52	<0.03	↓
烟酸Nicotinic acid	1.59	<0.03	↓
对羟基苯乙醇Tyrosol	1.72	<0.02	↓
烟酸Nicotinic acid	1.59	<0.03	↑