Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (11): 2815-2821.DOI: 10.6048/j.issn.1001-4330.2022.11.028
• Equine Exercise Physiology and Healthy Farming • Previous Articles Next Articles
WU Jinqiu1,2(), ZHANG Chong1, MENG Jun1,2,3, ZENG Yaqi1,2,3, Jianaer Nuerdun4, WANG Chuankun1,2, YUAN Xinxin1,2, WANG Tongliang1,2, YAO Xinkui1,2,3()
Received:
2022-01-11
Online:
2022-11-20
Published:
2022-12-28
Correspondence author:
YAO Xinkui
Supported by:
吴金秋1,2(), 张冲1, 孟军1,2,3, 曾亚琦1,2,3, 加娜尔・努尔顿4, 王川坤1,2, 袁鑫鑫1,2, 王彤亮1,2, 姚新奎1,2,3()
通讯作者:
姚新奎
作者简介:
吴金秋(1993-),女,新疆乌鲁木齐人,硕士研究生,研究方向为动物生产学,(E-mail)1610637765@qq.com
基金资助:
CLC Number:
WU Jinqiu, ZHANG Chong, MENG Jun, ZENG Yaqi, Jianaer Nuerdun, WANG Chuankun, YUAN Xinxin, WANG Tongliang, YAO Xinkui. Correlation Analysis between MCT1 Gene Polymorphismsand Speed Performance in Yili Horses[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2815-2821.
吴金秋, 张冲, 孟军, 曾亚琦, 加娜尔・努尔顿, 王川坤, 袁鑫鑫, 王彤亮, 姚新奎. 伊犁马MCT1基因多态性与速度性能的关联性分析[J]. 新疆农业科学, 2022, 59(11): 2815-2821.
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名称 Name | 引物序列(5’→3’) Sequences(5’→3’) | 退火温度 Annealing temperature | 产物大小 Size (bp) |
---|---|---|---|
MCT1-1 | F: CAATTCAGTTACATGAGAGCTAGGT R: ACATTTCACTTACCTCCACCATAC | 65℃ | 371 bp |
MCT1-2 | F: ACCAAGGTGTTTTCAGCCTACC R: CCAGGGAATAGCAAATAGCCAT | 342 bp |
Table 1 Primer information of gene
名称 Name | 引物序列(5’→3’) Sequences(5’→3’) | 退火温度 Annealing temperature | 产物大小 Size (bp) |
---|---|---|---|
MCT1-1 | F: CAATTCAGTTACATGAGAGCTAGGT R: ACATTTCACTTACCTCCACCATAC | 65℃ | 371 bp |
MCT1-2 | F: ACCAAGGTGTTTTCAGCCTACC R: CCAGGGAATAGCAAATAGCCAT | 342 bp |
组分 Component | 浓度 Concentration | 体积 Volume(μL) |
---|---|---|
Buffer I | 10× | 2.0 |
dNTP | 2.5 mM | 1.6 |
上游引物 Forward primer | 5.0 μL/M | 0.6 |
和下游引物 Reverse primer | 5.0 μL/M | 0.6 |
HS Taq | 5.0 U/μL | 0.2 |
DNA模板 DNA template | 20~50 ng/μL | 2.0 |
ddH2O | 13.0 |
Table 2 PCR reaction system
组分 Component | 浓度 Concentration | 体积 Volume(μL) |
---|---|---|
Buffer I | 10× | 2.0 |
dNTP | 2.5 mM | 1.6 |
上游引物 Forward primer | 5.0 μL/M | 0.6 |
和下游引物 Reverse primer | 5.0 μL/M | 0.6 |
HS Taq | 5.0 U/μL | 0.2 |
DNA模板 DNA template | 20~50 ng/μL | 2.0 |
ddH2O | 13.0 |
温度 Temperature | 时间 Time | 循环 Cycle |
---|---|---|
95℃预变性 | 5 min | |
95℃变性 | 30 s | |
65℃退火 | 30 s | 10 |
72℃延伸 | 30 s | |
95℃变性 | 30 s | |
55℃退火 | 30 s | 30 |
72℃延伸 | 30 s | |
72℃复延伸 | 10 min | |
4℃保存 | ∞ |
Table 3 PCR reaction conditions
温度 Temperature | 时间 Time | 循环 Cycle |
---|---|---|
95℃预变性 | 5 min | |
95℃变性 | 30 s | |
65℃退火 | 30 s | 10 |
72℃延伸 | 30 s | |
95℃变性 | 30 s | |
55℃退火 | 30 s | 30 |
72℃延伸 | 30 s | |
72℃复延伸 | 10 min | |
4℃保存 | ∞ |
编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号I.D. | 速度 Speed (m/s) |
---|---|---|---|---|---|---|---|---|---|
A1 | 12.71 | A11 | 10.80 | A21 | 12.45 | A31 | 10.80 | A41 | 13.00 |
A2 | 12.69 | A12 | 12.22 | A22 | 11.42 | A32 | 10.79 | A42 | 10.39 |
A3 | 12.67 | A13 | 11.99 | A23 | 11.44 | A33 | 10.49 | A43 | 13.06 |
A4 | 12.43 | A14 | 12.85 | A24 | 13.15 | A34 | 10.22 | A44 | 12.00 |
A5 | 12.10 | A15 | 12.56 | A25 | 12.93 | A35 | 10.30 | A45 | 12.85 |
A6 | 12.59 | A16 | 10.49 | A26 | 11.60 | A36 | 12.34 | A46 | 13.22 |
A7 | 11.27 | A17 | 12.00 | A27 | 10.24 | A37 | 12.12 | A47 | 11.93 |
A8 | 10.52 | A18 | 12.62 | A28 | 10.23 | A38 | 11.85 | A48 | 12.13 |
A9 | 12.17 | A19 | 11.34 | A29 | 10.33 | A39 | 12.23 | A49 | 12.55 |
A10 | 11.81 | A20 | 12.49 | A30 | 10.12 | A40 | 12.99 | A50 | 13.63 |
Table 4 Yili Horse 2000 m Race Speed
编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号 I.D. | 速度 Speed (m/s) | 编号I.D. | 速度 Speed (m/s) |
---|---|---|---|---|---|---|---|---|---|
A1 | 12.71 | A11 | 10.80 | A21 | 12.45 | A31 | 10.80 | A41 | 13.00 |
A2 | 12.69 | A12 | 12.22 | A22 | 11.42 | A32 | 10.79 | A42 | 10.39 |
A3 | 12.67 | A13 | 11.99 | A23 | 11.44 | A33 | 10.49 | A43 | 13.06 |
A4 | 12.43 | A14 | 12.85 | A24 | 13.15 | A34 | 10.22 | A44 | 12.00 |
A5 | 12.10 | A15 | 12.56 | A25 | 12.93 | A35 | 10.30 | A45 | 12.85 |
A6 | 12.59 | A16 | 10.49 | A26 | 11.60 | A36 | 12.34 | A46 | 13.22 |
A7 | 11.27 | A17 | 12.00 | A27 | 10.24 | A37 | 12.12 | A47 | 11.93 |
A8 | 10.52 | A18 | 12.62 | A28 | 10.23 | A38 | 11.85 | A48 | 12.13 |
A9 | 12.17 | A19 | 11.34 | A29 | 10.33 | A39 | 12.23 | A49 | 12.55 |
A10 | 11.81 | A20 | 12.49 | A30 | 10.12 | A40 | 12.99 | A50 | 13.63 |
β折叠(%) Beta turn | α螺旋 Alpha helix (%) | 无规 卷曲度 Random coil (%) | 伸展度 Extended strand (%) | |
---|---|---|---|---|
突变前 Before mutatin | 10.48 | 27.54 | 28.25 | 33.73 |
突变后 After mutatin | 10.79 | 28.57 | 27.30 | 33.33 |
Table 5 Prediction of secondary protein structure between before and after
β折叠(%) Beta turn | α螺旋 Alpha helix (%) | 无规 卷曲度 Random coil (%) | 伸展度 Extended strand (%) | |
---|---|---|---|---|
突变前 Before mutatin | 10.48 | 27.54 | 28.25 | 33.73 |
突变后 After mutatin | 10.79 | 28.57 | 27.30 | 33.33 |
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