Effect of Exogenous Salicylic Acid（SA）on the Leaf Anatomical Structure of Hami Melon under NaCl Stress

doi:10.6048/j.issn.1001-4330.2022.09.005

Abstract

Abstract:

【Objective】 To investigate the effect of exogenous salicylic acid （SA） on the leaf anatomical structure of Hami-melon under single salt stress （NaCl）. 【Methods】 In this experiment，the new Hami-melon variety Nasimi was used as the test material，and the optimum concentrations of NaCl and SA were screened by seed germination test. The changes of leaf thickness，upper and lower epidermal thickness，palisade and sponge thickness were analyzed，and the ratio of palisade thickness to sponge thickness，scattered rate （SR） and cell tightness rate（CTR） were calculated and correlation analysis was performed.【Results】 Under NaCl stress，the leaf thickness and upper and lower epidermal thickness of Hami-melon showed a trend of increasing，the palisade thickness were loosely arranged，and the leaf flesh gap was getting larger. After foliar spraying of SA，leaf upper and lower epidermal thickness，leaf thickness， palisade thickness，ratio of palisade thickness to sponge thickness increased，and spongy thickness and SR decreased. The palisade thickness and CTR were highly significantly positively correlated，and spongy thickness and SR were highly significantly positively correlated under the two treatments （P< 0.01）. 【Conclusion】 100 mmol/L NaCl was used as the stress concentration and 0.25 mmol/L SA was used as the mitigation concentration. SA treatment could alleviate the stress by regulating the indexes related to leaf epidermis and leaf flesh anatomical structure

Key words: Hami melon; salicylic acid; NaCl stress; leaf anatomical structure

摘要：

【目的】 研究外源水杨酸（SA）对单盐胁迫（NaCl）下哈密瓜叶片解剖结构的影响。【方法】 以哈密瓜新品种纳斯密为材料，采用种子发芽试验，筛选NaCl和SA最适浓度。运用石蜡切片技术，观察2个处理下哈密瓜幼苗叶片横切面，分析叶片厚度、上下表皮厚度及栅栏组织、海绵组织的变化，计算栅海比、叶片组织结构疏松度（SR）以及叶片组织结构紧密度（CTR），分析相关性。【结果】 NaCl胁迫下，哈密瓜叶片厚度、上下表皮厚度均出现了不断增加的趋势，栅栏组织排列疏松，叶肉间隙越来越大。而叶面喷施SA通过提高叶片上下表皮厚度、叶片厚度、栅栏组织厚度、栅海比，减小海绵组织厚度、SR，来缓解NaCl胁迫。2个处理下，栅栏组织厚度和CTR成极显著正相关，海绵组织厚度和SR成极显著正相关（P<0.01）。【结论】 100 mmol/LNaCl作为胁迫的浓度，0.25 mmol/LSA作为缓解浓度。SA处理可通过调节叶表皮和叶肉解剖结构的相关指标来缓解NaCl胁迫。

关键词: 哈密瓜, 水杨酸, 单盐胁迫, 叶片解剖结构

CLC Number:

S18
S652

YAN Miao, XIONG Tao, HUANG Quansheng, WU Ting, WU Haibo, ZHAO Zhun, HU Guozhi. Effect of Exogenous Salicylic Acid（SA）on the Leaf Anatomical Structure of Hami Melon under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2123-2129.

闫淼, 熊韬, 黄全生, 吴婷, 吴海波, 赵准, 胡国智. 外源水杨酸对单盐胁迫下哈密瓜叶片解剖结构的影响[J]. 新疆农业科学, 2022, 59(9): 2123-2129.

Figures/Tables 6

References 27

[1]	Albaladejo I, Meco V, Plasencia F, et al. Unravelling the strategies used by the wild tomato species Solanum prenelito confront salt stress;from leaf anatomical adaptations to molecular responses[J]. Environmental and Experimental Botany, 2017,(135):1-12.
[2]	Arif Y, Sami F, Siddiqui H, et al. Salicylic acid in relation to other phytohormones in plant:a study towards physiology and signal transduction under challenging environment[J]. Environmental and Experimental Botany, 2020,(175):104040.
[3]	Farahbakhsh H, Saiid M S. Effects of foliar application of salicylic acid on vegetative growth of maize under saline conditions[J]. African Journal of Plant Science, 2011, 5(10):575-578.
[4]	Shuang L, Dong Y, Xu L, et al. Effects of foliar applications of nitric oxide and salicylic acid on salt-induced changes in photosynthesis and antioxidative metabolism of cotton seedlings[J]. Plant Growth Regulation, 2014, 73(1):67-78. DOI URL
[5]	Dong C J, Wang X L, Shang Q M. Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings[J]. Scientia Horticulturae, 2011, 129(4):629-636. DOI URL
[6]	Horváth E, Csiszár J, Gallé A, et al. Hardening with salicylic acid induces concentration-dependent changes in abscisic acid biosynthesis of tomato under salt stress[J]. Journal of Plant Physiology, 2015,(183):54-63.
[7]	付乃鑫, 贺明荣, 诸葛玉平, 等. 外源SA对盐胁迫下冬小麦幼苗生长的缓解效应及其机理[J]. 中国农业大学学报, 2019, 24(3):10-17.
	FU Naixin, HE Mingrong, ZHUGE Yuping, et al. Effects and mechanisms of exogenous SA alleviating the growth of winter wheat seedlings under salt stress[J]. Journal of China Agricultural University, 2019, 24(3):10-17.
[8]	Mostofa M G, Fujita M, Tran L S P. Nitric oxide mediates hydrogen peroxide-and salicylic acid-induced salt tolerance in rice (Oryza sativa L.) seedlings[J]. Plant Growth Regulation, 2015, 77(3):265-277. DOI URL
[9]	UNIYAL R C, NAUTIYAL A R. Seed germination and seedling extension growth in Qugeinia dalbergioides Benth under water salinity stress[J]. New Forests, 1998,(16):265-272.
[10]	孟娜, 徐航, 魏明, 等. 叶面喷施烯效唑对盐胁迫下大豆幼苗生理及解剖结构的影响[J]. 西北植物学报, 2017, 37(10):1988-1995.
	MENG Na, XU Hang, WEI Ming, et al. Effect of Foliar Uniconazole Spraying under Salt Stress on Physiological and Anatomical Characteristics in Glycine max[J]. Acta Botanica Boreali-Occidentalia Sinica, 2017, 37(10):1988-1995.
[11]	孙彤彤, 武春成, 宋士清. 外源水杨酸(SA)、油菜素内酯(BR)浸种对Ca(NO₃)₂胁迫下黄瓜幼苗光合特性及叶片解剖结构的影响[J]. 江苏农业学报, 2019, 35(5):1184-1190.
	SUN Tongtong, WU Chun cheng, SONG Shiqing. Effects of exogenous salicylic acid(SA) and brassinosteroids(BR) soaking on photosynthetic characteristics and leaf anatomical structure of cucumber seedlings under Ca(NO₃)₂stress[J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(5):1184-1190.
[12]	王虹, 齐政, 张富春. 不同浓度盐胁迫下盐穗木叶片结构的比较观察[J]. 新疆农业学, 2016, 53(11):2098-2105.
	WANG Hong, QI Zheng, ZHANG Fuchun, et al. Effects of salt stress on leaf physiology and anatomical structure of two resistant apple rootstocks[J]. Xinjiang Agricultural Sciences, 2016, 53(11):2098-2105.
[13]	张霞, 孙旭春, 钟小仙, 等. 盐胁迫下象草叶片的显微结构[J]. 江苏农业学报, 2013, 29(2):278-282.
	ZHANG xia, SUN Xuchun, ZHONG Xiaoxian, et al. Microstructure of leaf of Pennisetum purpureum Schumach under salt stress[J]. Jiangsu Journal of Agricultural Sciences, 2013, 29(2):278-282.
[14]	曹栋栋, 陈珊宇, 秦叶波, 等. 水杨酸调控盐胁迫下羽衣甘蓝种子萌发的机理[J]. 植物学报, 2020, 55(1):49-61. DOI
	CAO Dongdong, CHEN Shanyu, QIN Yebo, et al. Mechanism of salicylic acid regulating seed germination of kale under Salt Stress[J]. Journal of Botany, 2020, 55(1):52-64.
[15]	金宁, 吕剑, 郁继华, 等. 外源硅对PEG渗透胁迫下黄瓜种子萌发及相关基因表达的影响[J]. 园艺学报, 2020, 47(1):41-52.
	JIN Ning, LV Jian, YU Jihua, et al. Effects of exogenous silicon on seed germination and expression of related genes in Cucumber under Osmotic stress[J]. Acta Horticultural Sinica, 2020, 47(1):41-52.
[16]	张德, 王双成, 张仲兴, 等. 盐胁迫对2种抗性苹果砧木叶片生理及解剖结构的影响[J]. 果树学报, 2021, 38(8):1275-1284.
	ZHANG De, WANG Shuangcheng, ZHANG Zhongxing, et al. Effects of salt stress on leaf physiology and anatomical structure of two resistant apple rootstocks[J]. Journal of Fruit Science, 2021, 38(8):1275-1284.
[17]	王喜涛, 周秀艳, 辛明, 等. 盐胁迫对甜瓜种子发芽的影响[J]. 北方园艺, 2014,(9):7-11.
	WANG Xitao, ZHOU Xiuyan, XIN Ming, et al. Effect of salt stress on Seed Germination of melon[J]. North Journal of Horticulture, 2014,(9):7-11.
[18]	张贝贝, 樊佳茹, 王景荣, 等. 模拟盐渍化对甜瓜种子萌发和幼苗生理特性的影响[J]. 热带作物学报, 2020, 41(5):912-920.
	ZHANG Beibei, FAN Jiaru, WANG Jingrong, et al. Effects of Simulated Salinization on Seed Germination and Physiological Characteristics of Muskmelon Seedlings[J]. Journal of Tropical Crops, 2020, 41(5):912-920.
[19]	LOICR, MAYAB, Romain H, CAROLINE R, et al. Proteomic Investigation of the Effect of Salicylic Acid on Arabidopsis Seed Germination and Establishment of Early Defense Mechanisms[J]. Plant Physiology, 2006, 141(3):910-923. DOI PMID
[20]	马广民, 赵孟如, 怀婷婷, 等. 水杨酸对盐胁迫下西瓜种子萌发及幼苗生长的影响[J]. 中国果树, 2020,(6):36-40.
	MA Guangming, ZHAO Mengru, HUAI Tingting et al. Effects of salicylic acid on seed germination and seedling growth of watermelon under salt stress[J]. Chinese journal of Fruit Trees, 2020,(6):36-40.
[21]	Castro-Diez P, Puyravaud J P, Cornelissen J H C. Leaf structure and anatomy as related to leaf mass per area variation in seedings of a wide range of woody plant species and types[J]. Oecologia, 2000, 124(4):476-486. DOI PMID
[22]	韦存虚, 张军, 王建军, 等. 星星草营养器官适应盐胁迫的结构特征[J]. 植物资源与环境学报, 2006,(1):51-56.
	WEI Cunxu, ZHANG jun, WANG Jianjun, et al. Observation on structural characters of vegetative organs of Puccinellia tenuiflora under salt stress[J]. Journal of Plant Resources and Environment, 2006,(1):51-56.
[23]	王斌, 巨波, 赵慧娟, 等. 不同盐梯度处理下沼泽小叶桦的生理特征及叶片结构[J]. 林业科学, 2011, 47(10):29-36.
	WANG Bin, JU Bo, ZHAO Huijuan, et al. Photosynthetic Performance and Variation in Leaf Anatomic Structure of Betula microphyla var.paludosa under Different Saline Conditions[J]. Scientia Silvae Sinicae, 2011, 47(10):29-36.
[24]	田晨霞, 张咏梅, 王凯, 等. 紫花苜蓿组织解剖结构对NaHCO₃盐碱胁迫的响应[J]. 草业学报, 2014, 23(5):133-142.
	TIAN Chenxia, ZHANG Yongmei, WANG Kai, et al. The anatomical structure responses in alfalfa to salinity-alkalinity stress of NaHCO₃[J]. Acta Prataculturae Sinica, 2014, 23(5):133-142.
[25]	Chartzoulakis K, Patakas A, Kofidis G, et al. Water stress affects leaf anatomy,gas exchange,water relationsand growth of two avocado cultivars[J]. Scientia Horticulturae, 2002, 95(1):39-50. DOI URL
[26]	武春霞, 陈兴华, 杨静慧, 等. 盐胁迫下两种樱桃叶片解剖结构变化研究[J]. 落叶果树, 2014, 43(5):89-91.
	WU Chunxia, CHEN Xinghua, YANG Jinghui, et al. Study on changes of leaf anatomical structure of two kinds of cherry under salt stress[J]. Luoye Guoshu, 2014, 43(5):89-91.
[27]	许盼云, 李春兰, 马萍, 等. 基于叶片显微结构筛选耐盐碱苹果砧木[J]. 分子植物育种:1-17.(网络首发)
	XU Panyun, LI Chunlan, MA Ping, et al. Screening of Salt-Alkali Tolerant Apple Rootstocks Based on Leaf Microstructure[J]. Molecular Plant Breeding:1-17.

NaCl （mmol/L）	发芽势 Germination energy（%）	发芽率 Germination rate（%）	芽苗全长 Seeding length（cm）	鲜质量 Fresh weight（g）
0（H₂O）	81.67±4.41^a	96.67±1.67^a	8.55±0.51^a	0.19±0.01^a
50	90.00±0.00^a	98.33±1.67^a	5.81±0.61^b	0.14±0.01^b
100	55.00±7.64^b	73.33±10.14^b	2.03±0.38^c	0.08±0.01^c
150	10.00±7.64^c	15.00±12.58^c	0.79±0.35^d	0.05±0.01^b
200	0.00±0.00^c	0.00±0.00^c	0.00±0.00^d	0.00±0.00 ^e

NaCl （mmol/L）	发芽势 Germination energy（%）	发芽率 Germination rate（%）	芽苗全长 Seeding length（cm）	鲜质量 Fresh weight（g）
0（H₂O）	81.67±4.41^a	96.67±1.67^a	8.55±0.51^a	0.19±0.01^a
50	90.00±0.00^a	98.33±1.67^a	5.81±0.61^b	0.14±0.01^b
100	55.00±7.64^b	73.33±10.14^b	2.03±0.38^c	0.08±0.01^c
150	10.00±7.64^c	15.00±12.58^c	0.79±0.35^d	0.05±0.01^b
200	0.00±0.00^c	0.00±0.00^c	0.00±0.00^d	0.00±0.00 ^e

NaCl （mmol/L）	SA （mmol/L）	发芽势 Germination energy（%）	发芽率 Germination rate（%）	芽苗全长 Seeding length（cm）	鲜质量 Fresh weight（g）
0	0	88.83±1.67^a	96.67±1.67^a	15.75±1.06^a	0.31±0.02^a
100	0	55.00±7.64^b	73.33±10.14^b	2.03±0.38^c	0.08±0.01^c
100	0.25	86.67±3.33^a	93.33±3.33^a	13.68±0.96^a	0.28±0.01^a
100	2.5	70.00±2.89^b	78.33±1.67^a	7.4±0.92^b	0.14±0.02^b
100	5	65.00±2.89^b	70.00±2.89^b	3.239±0.52^c	0.10±0.01^c
100	10	56.67±6.01^b	61.67±8.33^b	1.41±0.22^c	0.06±0.01^c

NaCl （mmol/L）	SA （mmol/L）	发芽势 Germination energy（%）	发芽率 Germination rate（%）	芽苗全长 Seeding length（cm）	鲜质量 Fresh weight（g）
0	0	88.83±1.67^a	96.67±1.67^a	15.75±1.06^a	0.31±0.02^a
100	0	55.00±7.64^b	73.33±10.14^b	2.03±0.38^c	0.08±0.01^c
100	0.25	86.67±3.33^a	93.33±3.33^a	13.68±0.96^a	0.28±0.01^a
100	2.5	70.00±2.89^b	78.33±1.67^a	7.4±0.92^b	0.14±0.02^b
100	5	65.00±2.89^b	70.00±2.89^b	3.239±0.52^c	0.10±0.01^c
100	10	56.67±6.01^b	61.67±8.33^b	1.41±0.22^c	0.06±0.01^c

胁迫时间 Stress time（d）	处理 Treatment	叶片厚度 Thickness of leaf/µm	上表皮厚度 Thickness of upper epidermis/µm	下表皮厚度 Thickness of lower epidermis/µm
4	水杨酸 SA	184.60±3.21^b	17.43±3.67^a	12.52±2.16^a
4	单盐胁迫 NaCl	175.96±4.09^b	10.50±4.83^b	5.62±0.70^c
6	水杨酸 SA	211.68±4.96^a	12.52±3.73^ab	8.82±1.80^b
6	单盐胁迫 NaCl	179.54±3.64^b	12.51±1.48^ab	7.54±0.99^bc