Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

doi:10.6048/j.issn.1001-4330.2022.08.022

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 2007-2013.DOI: 10.6048/j.issn.1001-4330.2022.08.022

• Plant Protection·Agricultural Product Analysis and Detection·Agricultural Equipment Engineering and Mechanization • Previous Articles Next Articles

Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

WANG Xiaoli¹(), HAN Rui¹, ZHANG Quancheng¹, ZHAO Pengcheng², WANG Jungang¹()

1. College of Agriculture/Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization, Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi Xinjiang 832003, China
2. Xinjiang Tianhe Jiaxin Agricultural Technology Co., Ltd., Urumqi 830000,China

Received:2021-10-15 Online:2022-08-20 Published:2022-10-01
Correspondence author: WANG Jungang
Supported by:
Regional Science Foundation projects “The Cotton Defense Response Induced by Acyrthosiphom gossypii on Interspecific Competition between Acyrthosiphom gossypii and Aphis gossypii ”(31660519);Regional Science Foundation projects “Biochemical Mechanism of Cotton Resistant to Aphis gossypii by mepiquat chloride ”(31260435)

不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

王小丽¹(), 韩睿¹, 张全成¹, 赵鹏程², 王俊刚¹()

1.石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子 832003
2.新疆天禾嘉信农业科技有限公司,乌鲁木齐 830000

通讯作者: 王俊刚
作者简介:王小丽（1997-）,女,新疆昌吉人,硕士研究生,研究方向为植物保护,（E-mail） 1436647660@qq.com
基金资助:
国家自然科学基金“棉长管蚜诱导棉花防御反应对棉长管蚜和棉蚜种间竞争影响”(31660519);国家自然科学基金“缩节胺调控棉花防御棉蚜的生化机制研究”(31260435)

Abstract

Abstract:

【Objective】Objective to explore the difference of three enzyme activities and drug resistance of different geographical populations of Aphis gossypii in Xinjiang. 【Methods】 The activities of carboxylesterase, glutathione-S-transferase and acetylcholinesterase of Aphis gossypii and laboratory sensitive populations in Korla, Wujiaqu, Shihezi, Kuitun and Hami of Xinjiang were determined, and the relationship between the activities of the three enzymes and imidacloprid resistance was analyzed. 【Results】 The specific activity ratios of acetylcholinesterase and glutathione-S-transferase in Korla, Wujiaqu, Shihezi, Kuitun and Hami were 4.2, 2.7, 2.3, 2.5, 2.1 times and 2.3, 1.5, 1.3, 2.5, 2.1 times, respectively（P < 0.05）; the specific activity ratios of carboxylesterase were 6.5, 4.2, 3.4, 2.7, 1.2 times, except Hami, the other areas were significantly higher than 1.2 times There was a certain correlation between two key detoxification enzymes and one key neurotransmitter enzyme of Aphis gossypii and drug resistance.【Conclusion】 The results showed that the activities of three kinds of enzymes in different populations were significantly higher than those in sensitive populations（P <0.05）, and there were differences among them. Carboxylesterase may play an important role in the process of Aphis gossypii feeling imidacloprid stress.

Key words: Aphis gossypii; carboxylesterase; acetylcholinesterase; glutathione-S-transferase; drug resistance

摘要：

【目的】研究新疆不同地理种群棉蚜3种酶的活性及抗药性的差异。【方法】测定新疆库尔勒、五家渠、石河子、奎屯、哈密地区棉蚜和实验室敏感种群羧酸酯酶、谷胱甘肽-S-转移酶、乙酰胆碱酯酶活性,并分析3种酶活性与吡虫啉抗性之间的关系。【结果】库尔勒、五家渠、石河子、奎屯和哈密地区乙酰胆碱酯酶和谷胱甘肽-S-转移酶的比活力比值分别为4.2、2.7、2.3、2.5、2.1倍和2.3、1.5、1.3、2.5、2.1倍,差异显著（P<0.05）;羧酸酯酶比活力比值为6.5、4.2、3.4、2.7、1.2倍,除哈密地区相对接近外,其余地区均明显高于1.0,差异显著（P<0.05）,2种棉蚜关键解毒酶和1种神经传导关键性酶与抗药性之间存在一定相关性。【结论】各地理种群棉蚜3种酶活性均显著高于敏感种群（P<0.05）,且彼此之间存在差异性,羧酸酯酶在棉蚜感受到吡虫啉杀虫剂胁迫时极有可能起到重要作用。

关键词: 棉蚜, 羧酸酯酶, 乙酰胆碱酯酶, 谷胱甘肽-S-转移酶, 抗药性

CLC Number:

WANG Xiaoli, HAN Rui, ZHANG Quancheng, ZHAO Pengcheng, WANG Jungang. Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 2007-2013.

王小丽, 韩睿, 张全成, 赵鹏程, 王俊刚. 不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性[J]. 新疆农业科学, 2022, 59(8): 2007-2013.

Figures/Tables 4

References 30

[1]	帕提玛·乌木尔汗, 郭佩佩, 马少军, 等. 新疆地区棉蚜田间种群对10种杀虫剂的抗性[J]. 植物保护, 2019, 45(6): 273-278.
	Pattima Wumuerhan, GUO Pei, MA Shaojun, et al. Resistance of Aphis gossypii field population to 10 insecticides in Xinjiang[J]. Plant protection, 2019, 45(6): 273-278.
[2]	何玲, 王李斌. 新烟碱类杀虫剂吡虫啉的抗性研究进展[J]. 农药科学与管理, 2018, 39(1): 27-31.
	HE Ling, WHANG Libin. Research progress on the resistance of new nicotine insecticide imidacloprid[J]. Pesticide science and management, 2018, 39(1): 27-31.
[3]	刘刚. 河北省棉蚜对新烟碱类杀虫剂均产生了高至极高的抗药性水平[J]. 农药市场信息, 2018,(26):50.
	LIU Gang. Aphid gossypii had high to very high resistance to new nicotine insecticides in Hebei Province[J]. Pesticide market information, 2018(26): 50.
[4]	金永玲. 大垫尖翅蝗抗药性检测及其对丁烯氟虫腈代谢抗性机理研究[D]. 沈阳: 沈阳农业大学, 2016.
	JIN Yongling. Detection of insecticide resistance and metabolic resistance mechanism to butenfipronil[D]. Shenyang: Shenyang Agricultural University, 2016.
[5]	李永强, 安凤秋, 苏立德, 等. 棉铃虫酯酶两个位点突变对三种杀虫剂代谢活性的影响[J]. 植物保护学报, 2012, 39(3):271-276.
	LI Yongqiang, AN Fengqiu, Su Lide, et al. Effects of two site mutations of esterase from Helicoverpa armigera on the metabolic activities of three insecticides[J]. Acta botanical protection, 2012, 39(3): 271-276
[6]	郭晓军. 我国烟粉虱种群遗传分化及对杀虫剂的解毒代谢机制研究[D]. 北京: 中国农业大学, 2015.
	GUO Xiaojun. Study on genetic differentiation of Bemisia tabaci population and its detoxification metabolism mechanism to insecticides[D]. Beijing: China Agricultural University, 2015.
[7]	黄诚华, 姚洪渭, 叶恭银, 等. 氟虫腈亚致死剂量处理对二化螟和大螟幼虫体内解毒酶系活力的影响[J]. 中国水稻科学, 2006(4):447-450.
	HUANG Chenghua, YAO Hongwei, ye Gongyin, et al. Effect of sublethal fipronil on detoxification enzyme activities of Chilo suppressalis and Chilo suppressalis[J]. Rice science of China, 2006(4): 447-450.
[8]	胡珍娣. 小菜蛾对氯虫苯甲酰胺的抗药性及其解毒机理研究[D]. 北京: 中国农业大学, 2016.
	HU Zhendi. Study on resistance and detoxification mechanism of Plutella xylostella to chlorantraniliprole[D]. Beijing: China Agricultural University, 2016.
[9]	黄钰淞, 梁文涛, 谢佳燕. 麦二叉蚜吡虫啉抗性选育及其3种酶活性变化研究[J]. 武汉轻工大学学报, 2014, 33(3): 13-15+49.
	HUANG Yusong, LIANG Wentao, XIE Jiayan. Study on imidacloprid resistance breeding and changes of three enzyme activities of Schizaphisgraminum[J]. Journal of Wuhan University of light industry, 2014, 33(3): 13-15+49.
[10]	郭天凤, 史雪岩, 高希武, 等. 棉蚜吡虫啉、啶虫脒不同品系解毒酶活性测定和增效剂作用的研究[J]. 环境昆虫学报, 2014, 36(3): 388-394.
	GUO Tianfeng, SHI Xueyan, GAO Xiwu, et al. Determination of detoxification enzyme activity and synergist effect of imidacloprid and Acetamiprid in different strains of Aphis gossypii[J]. Acta entomology Sinica, 2014, 36(3): 388-394.
[11]	郑巨云, 王俊铎, 梁亚军, 等. 新疆棉花种业现状及展望[J]. 中国棉花, 2020, 47(6): 1-8.
	ZHENG juyun, WANG junduo, LIANG Yajun, et al. Status and Prospect of cotton seed industry in Xinjiang[J]. China cotton, 2020, 47(6): 1-8.
[12]	梁彦, 张帅, 邵振润, 等. 棉蚜抗药性及其化学防治[J]. 植物保护, 2013, 39(5):70-80.
	LIANG Yan, ZHANG Shuai, SHAO zhenrun, et al. Insecticide resistance of Aphis gossypii and its chemical control[J]. Plant protection, 2013, 39(5): 70-80.
[13]	Van Asperen K. A study of housefly esterases by means of a sensitive colorimetric method[J]. Journal of Insect Physiology, 1962, 8(4): 401-416. DOI URL
[14]	高希武, Gorun. 等. 改进的Ellman胆碱酯酶活性测定方法介绍[J]. 昆虫知识, 1987,(4): 245-246.
	GAO Xiwu, Gorun, et al. Improved Ellman cholinesterase activity determination method[J]. Entomology, 1987,(4): 245-246.
[15]	Habig WH. In:Willian BJ, ed. Method in Enzymology[M]. NewYork: Academic Press, 1981,(77) :398-405.
[16]	Bradford Marion M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72(1-2): 248-254. DOI URL
[17]	史晓斌, 石绪根, 王红艳, 等. 抗吡虫啉棉蚜对其他新烟碱类药剂的交互抗性及相关酶的活性变化[J]. 昆虫学报, 2011, 54(9):1027-1033.
	SHI Xiaobin, SHI Xugen, WANG Hongyan, et al. Cross resistance of imidacloprid resistant Aphis gossypii to other neonicotinoids and changes of related enzyme activities[J]. Acta Entomologica Sinica, 2011, 54(9): 1027-1033.
[18]	赵鹏程, 李焱, 闫文静, 等. 新疆棉蚜不同地理种群对杀虫剂的敏感性[J]. 石河子大学学报(自然科学版), 2018, 36(2): 159-163.
	ZHAO Pengcheng, LI Yan, YAN Wenjing, et al. Sensitivity of different geographic populations of Aphis gossypii to insecticides in Xinjiang[J]. Journal of Shihezi University(Natural Science Edition), 2018, 36(2): 159-163.
[19]	张国福, 李琳琳, 李本杰, 等. 山东省不同地区棉蚜对新烟碱类杀虫剂的抗药性检测及酶抑制剂的增效作用研究[J]. 农药学学报, 2014, 16(6):673-680.
	ZHANG Guofu, LI Linlin, LI Benjie, et al. Resistance detection of cotton aphid to neonicotinoids and synergistic effect of enzyme inhibitors in different areas of Shandong Province[J]. Acta agrologica Sinica, 2014, 16(6): 673-680.
[20]	杨柳, 伍绍龙, 柳傲, 等. 湖南宁乡烟区烟蚜的抗性监测及其相关酶的活性测定[J]. 环境昆虫学报, 2018, 40(6): 1404-1412.
	YANG Liu, WU Shaolong, LIU Ao, et al. Resistance monitoring and enzyme activity determination of Myzus persicae in Ningxiang tobacco growing area of Hunan Province[J]. Acta entomology Sinica, 2018, 40(6): 1404-1412.
[21]	史晓斌, 石绪根, 王红艳, 等. 抗吡虫啉棉蚜对其他新烟碱类药剂的交互抗性及相关酶的活性变化[J]. 昆虫学报, 2011, 54(9): 1027-1033.
	SHI Xiaobin, SHI Xugen, WANG Hongyan, et al. Cross resistance of imidacloprid resistant Aphis gossypii to other new nicotine insecticides and changes of related enzyme activities[J]. Acta Entomology, 2011, 54(9): 1027-1033.
[22]	王晓冰. 绿盲蝽抗呋虫胺品系的汰选与其解毒酶的研究[D]. 河南: 河南农业大学, 2018.
	WANG Xiaobing. Study on the selection and detoxification enzyme of furadimer resistant strains of Apolygus lucorμm[D]. Henan: Henan Agricultural University, 2018.
[23]	杨焕青, 王开运, 王红艳, 等. 抗吡虫啉棉蚜种群对吡蚜酮等药剂的交互抗性及施药对其生物学特性的影响[J]. 昆虫学报, 2009, 52(2): 175-182.
	YANG Huanqing, WANG Kaiyun, WANG Hongyan, et al. Cross resistance of imidacloprid resistant Aphis gossypii population to insecticides such as Pymetrozine and the effect of pesticide application on its biological characteristics[J]. Acta Entomologica Sinica, 2009, 52(02): 175-182.
[24]	潘文亮, 党志红, 高占林. 棉蚜抗吡虫啉品系和敏感品系主要解毒酶活性比较[J]. 昆虫学报, 2003,(6): 793-796.
	PAN Wenliang, DANG Zhihong, GAO Zhanlin. Comparison of main detoxification enzyme activities between imidacloprid resistant and Susceptible Aphis gossypii strains[J]. Acta Entomologica, 2003,(6): 793-796.
[25]	崔丽, 张靖, 齐浩亮, 等. 我国棉花主产区棉蚜对吡虫啉的抗性监测及抗性机理[J]. 昆虫学报, 2016, 59(11): 1246-1253.
	CUI Li, ZHANG Jing, QI Haoliang, et al. Resistance monitoring and resistance mech Aphis gossypii to imidacloprid in main cotton producing areas of China[J]. Acta Entomology, 2016, 59(11): 1246-1253.
[26]	党志红, 安静杰, 高占林, 等. 河北省不同地区棉蚜种群对6种杀虫剂抗药性与种群适合度[J]. 植物保护, 2019, 45(3): 111-114.
	DANG Zhihong, JINGJING Jie, GAO Zhanlin, et al. Resistance and population suitability of Aphis gossypii populations to six insecticides in different regions of Hebei Province[J]. Plant protection, 2019, 45(3): 111-114.
[27]	LI Cui, Haoliang Qi, Daibin Yang, et al. Cycloxaprid: A novel cis -nitromethylene neonicotinoid insecticide to control imidacloprid-resistant cotton aphid(Aphis gossypii)[J]. Pesticide Biochemistry and Physiology, 2016, 132.
[28]	Grant A Herron, Lewis J Wilson. Can resistance management strategies recover insecticide susceptibility in pests?: a case study with cotton aphid Aphis gossypii(Aphididae: Hemiptera) in Australian cotton[J]. Austral Entomology, 2017, 56(1).
[29]	Akira Matsuura, Masakazu Nakamura. Development of neonicotinoid resistance in the cotton aphid Aphis gossypii(Hemiptera: Aphididae) in Japan[J]. Applied Entomology and Zoology, 2014, 49(4).
[30]	Grant A Herron, Brendan J Langfield, Daniel R Bogema, et al. Baseline susceptibility and cross‐resistance in Aphis gossypii G lover(Aphididae: Hemiptera) to phorate and sulfoxaflor[J]. Austral Entomology, 2014, 53(1).

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	CarE比活力比值 Dynamic ratio of CarE
SS	0.236±0.006 ^d	1.0
奎屯 Kurtun	0.647±0.116 ^c	2.7
石河子 SHi hezi	0.808±0.002 ^bc	3.4
哈密 Ha mi	0.284±0.002 ^d	1.2
五家渠 Wujaqu	0.992±0.153 ^b	4.2
库尔勒 Kuerle	1.359±0.119 ^a	6.5

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	CarE比活力比值 Dynamic ratio of CarE
SS	0.236±0.006 ^d	1.0
奎屯 Kurtun	0.647±0.116 ^c	2.7
石河子 SHi hezi	0.808±0.002 ^bc	3.4
哈密 Ha mi	0.284±0.002 ^d	1.2
五家渠 Wujaqu	0.992±0.153 ^b	4.2
库尔勒 Kuerle	1.359±0.119 ^a	6.5

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	AchE比活力比值 Dynamic ratio of AchE
SS	2.997±0.034 ^f	1.0
奎屯 Kurtun	7.346±0.030 ^c	2.5
石河子 SHi hezi	6.950±0.028 ^d	2.3
哈密 Ha mi	6.146±0.016 ^e	2.1
五家渠 Wujaqu	8.196±0.044 ^b	2.7
库尔勒 Kuerle	12.570±0.051 ^a	4.2

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	AchE比活力比值 Dynamic ratio of AchE
SS	2.997±0.034 ^f	1.0
奎屯 Kurtun	7.346±0.030 ^c	2.5
石河子 SHi hezi	6.950±0.028 ^d	2.3
哈密 Ha mi	6.146±0.016 ^e	2.1
五家渠 Wujaqu	8.196±0.044 ^b	2.7
库尔勒 Kuerle	12.570±0.051 ^a	4.2

棉蚜种群 Population	CarE比活力 Specific activity of CarE （U /mgpro-min）	GST比活力比值 Dynamic ratio of GST
SS	3.986±0.035 ^f	1.0
奎屯 Kurtun	10.080±0.343 ^a	2.5
石河子 SHi hezi	5.242±0.035 ^e	1.3
哈密 Ha mi	8.445±0.323 ^c	2.1
五家渠 Wujaqu	6.143±0.097 ^d	1.5
库尔勒 Kuerle	9.150±0.106 ^b	2.3

Study on the Differences of Three key Enzyme Activities of Different Geographical Populations of Aphis gossypii in Xinjiang and Their Correlation with Resistance

不同地理种群棉蚜三种关键性酶活差异及其与抗性相关性

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