Effects of Seed Dressing with Thiamethoxam on Detoxifying Enzymes of Aphis gossypii and Acyrthosiphom gossypii

doi:10.6048/j.issn.1001-4330.2021.09.015

Abstract

Abstract: 【Objective】 To ascertain the effect of detoxification enzyme of Acyrthosiphom gossypii and Aphisgossypii after feeding with cotton seed dressed by thiamethoxam, and further analyze their resistance mechanism to the drug.【Methods】 Cotton seed was dressed with thiamethoxam by 2 g/kg and 4 g/kg, and the changes of detoxifying enzyme activities in Aphis gossypii and Acyrthosiphongossypii were determined by biochemical methods after inoculating 0 h, 2 h, 6 h, 12 h and 24 h.【Result】 Except acetyl cholinesterase (AChE) in Acyrthosiphon gossypii , at low dose, the activities of AChE, carboxylesterase (CarE), cytochrome P450 (CYP450) and glutathione-S-transferase (GSTs) in Aphis gossypii and Acyrthosiphon gossypii were induced first and then inhibited. In the test, the activities of AChE, CarE, CYP450, GSTs of Aphis gossypii were 4.62-fold, 2.51-fold, 1.08-fold and 1.51-fold, respectively compared with the Acyrthosiphon gossypi. The relative activities of AChE, CYP450 and GSTs between Aphis gossypii and Acyrthosiphon gossypii were increased in a short time, and then decreased with time, while CarE showed a decrease → increase → decrease. At high dose, the relative activity ratio of CYP450 and CarE were increased → decreased → increased.【Conclusion】 Low dose of thiamethoxam seed dressing can induce CarE and CYP450 of aphids for a short time, and high dose can inhibit each detoxification enzyme. CarE and CYP450 may be the key enzymes for detoxification metabolism of thiamethoxam by aphids, and Aphis gossypii has a better suitability than Acyrthosiphon gossypi to seed dressing cotton.

Key words: Thiamethoxam; Acyrthosiphom gossypii; Aphis gossypii; detoxifying enzyme

摘要： 【目的】研究棉蚜和棉长管蚜取食噻虫嗪包衣棉花后解毒酶活性的变化,分析二者的抗药性机制。【方法】使用2、4 g/kg噻虫嗪包衣棉花,三叶期时采用生化方法测定取食0、2、6、12、24 h后棉蚜和棉长管蚜体内解毒酶的活性变化。【结果】取食噻虫嗪包衣棉花叶片后,低剂量处理组的棉蚜和棉长管蚜体内乙酰胆碱酯酶(AChE)、羧酸酯酶(CarE)、细胞色素P450(CYP450)、谷胱甘肽-S-转移酶(GSTs)的活性除棉长管蚜AChE外均先被诱导,而后被抑制,不同酶受影响程度不同。高剂量处理组棉蚜和棉长管蚜体内各解毒酶均被抑制;供试棉蚜AChE、CarE、CYP450、GSTs的相对活力分别为棉长管蚜的4.62、2.51、1.08、1.51倍。低剂量处理后棉蚜和棉长管蚜间AChE、CYP450、GSTs相对活力比短时内均升高,随时间延续又有所下降,而CarE则表现为降低→升高→降低的趋势。高剂量处理后CYP450与CarE的相对活力比均表现为升高→降低→升高的变化趋势。【结论】噻虫嗪低剂量种子包衣对蚜虫CarE和CYP450有短时的诱导作用,高剂量对各解毒酶均表现出抑制作用,CarE与CYP450可能是蚜虫对噻虫嗪解毒代谢的关键酶,棉蚜较棉长管蚜对药剂包衣棉花适宜能力更强。

关键词: 噻虫嗪, 棉蚜, 棉长管蚜, 解毒酶

CLC Number:

S435.622

MA Zhenghui, SU Yue, MA Xue, YANG He, HOU Guohui, CHEN Bingrun, HAN Xu, YAO Yongsheng. Effects of Seed Dressing with Thiamethoxam on Detoxifying Enzymes of Aphis gossypii and Acyrthosiphom gossypii[J]. Xinjiang Agricultural Sciences, 2021, 58(9): 1685-1693.

麻正辉, 苏悦, 马雪, 羊河, 侯国辉, 陈柄润, 韩旭, 姚永生. 噻虫嗪种子包衣棉花对棉蚜和棉长管蚜解毒酶活性的影响[J]. 新疆农业科学, 2021, 58(9): 1685-1693.

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