Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (9): 2310-2317.DOI: 10.6048/j.issn.1001-4330.2022.09.027
• Animal Husbandry Veterinarian·Agricultural Equipment Engineering and Mechanization·Agricultural Eeconomy • Previous Articles Next Articles
FAN Xue1,2(), SHAO Wei1, ZHAO Yankun2(), DU Xiaohui1, CHEN He2, WANG Fulan2, WANG Shuai2
Received:
2021-02-26
Online:
2022-09-20
Published:
2023-01-16
Correspondence author:
ZHAO Yankun
Supported by:
范雪1,2(), 邵伟1, 赵艳坤2(), 杜晓慧1, 陈贺2, 王富兰2, 王帅2
通讯作者:
赵艳坤
作者简介:
范雪(1995-),女,新疆奇台人,硕士研究生,研究方向为动物生产,(E-mail)1549418390@qq.com
基金资助:
CLC Number:
FAN Xue, SHAO Wei, ZHAO Yankun, DU Xiaohui, CHEN He, WANG Fulan, WANG Shuai. Differential Expression of Drug Resistance and Virulence Genes in Streptococcus agalactiae Isolated from Cattle[J]. Xinjiang Agricultural Sciences, 2022, 59(9): 2310-2317.
范雪, 邵伟, 赵艳坤, 杜晓慧, 陈贺, 王富兰, 王帅. 牛源无乳链球菌耐药性与毒力基因的表达量差异分析[J]. 新疆农业科学, 2022, 59(9): 2310-2317.
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基因名称 Gene name | 引物序列 Primer sequence | 目的片段 Target fragment(bp) |
---|---|---|
pavA | F:TTCCCATGATTTCAACAACAAG | 495 |
R:AACCTTTTGACCATGAATTGGTA | ||
scpB | F: AGTTGCTTCTTACAGCCCAGA | 567 |
R: GGCGCAGACATACTAGTTCCA | ||
rib | F:CAGGAAGTGCTGTTACGTTAAAC | 306 |
R: CGTCCCATTTAGGGTCTTCC | ||
bac | F:CTATTTTTGATATTGACAATGCAA | 592 |
R:GTCGTTACTTCCTTGAGATGTAAC | ||
cylE | F: TGACATTTACAAGTGACGAAG | 248 |
R: TTGCCAGGAGGAGAATAGGA | ||
bca | F:TAACAGTTATGATACTTCACAGAC | 320 |
R:ACGACTTTCTTCCGTCCACTTAGG | ||
cfb | F: GCTGTTTGAAGTGCTGCTTG | 288 |
R: GACTTCATTGCGTGCCAAC | ||
lmb | F: AGTCAGCAAACCCCAAACAG | 397 |
R: GCTTCCTCACCAGCTAAAACG | ||
hylB | F: CACCAATCCCCACTCTACTA | 346 |
R: TGTGTCAAACCATCTATCAG | ||
fbsA | F:AGAGCCAAGTAGGTCAACTTATAG | 290 |
R: TTCATTGCGTCTCAAACCG | ||
bibA | F:AACCAGAAGCCAAGCCAGCAACC | 127 |
R: AGTGGACTTGCGGCTTCACCC | ||
cspA | F: GGTCGCGATAGAGTTTCTTCCGC | 104 |
R: AACGCCTGGGGCTGATTTGGC | ||
sip | F: TCTCTCAATACAATTTCGGAAG | 867 |
R: GTGGTCATAGTGGTTGGCAGT | ||
iagA | F: CGGGATTGATCTAAGTCGCT | 459 |
R: CCATCAACATCAGTCGCTAA | ||
ermA | F: TCTAAAAAGCATGTAAAAGAA | 645 |
R: CTTCGATAGTTTATTAATATTAGT | ||
ermB | F: ATTGGAACAGGTAAAGGGC | 442 |
R: GAACATCTGTGGTATGGCG | ||
ermC | F: TCAAAACATAATATAGATAAA | 642 |
R: GCTAATATTGTTTAAATCGTCAAT | ||
sul1 | F: CATTGCCTGGTTGCTTCAT | 238 |
R: ATCCGACTCGCAGCATTT | ||
sul2 | F: CATCATTTTCGGCATCGTC | 793 |
R: TCTTGCGGTTTCTTTCAGC | ||
sul3 | F: AGATGTGATTGATTTGGGAGC | 443 |
R: TAGTTGTTTCTGGATTAGAGCCT | ||
gyrA | F: GACAAGTGAAATGAAAACGAG | 474 |
R: CGCTCCATTGACTAATAAATTAGG | ||
parC | F: CAAAACATGTCCCTTGAGGA | 568 |
R: CTAGCTTTGGGATGATCAATCAT |
Table 1 Primer sequence of virulence gene and drug resistance gene of Streptococcus agalactiae
基因名称 Gene name | 引物序列 Primer sequence | 目的片段 Target fragment(bp) |
---|---|---|
pavA | F:TTCCCATGATTTCAACAACAAG | 495 |
R:AACCTTTTGACCATGAATTGGTA | ||
scpB | F: AGTTGCTTCTTACAGCCCAGA | 567 |
R: GGCGCAGACATACTAGTTCCA | ||
rib | F:CAGGAAGTGCTGTTACGTTAAAC | 306 |
R: CGTCCCATTTAGGGTCTTCC | ||
bac | F:CTATTTTTGATATTGACAATGCAA | 592 |
R:GTCGTTACTTCCTTGAGATGTAAC | ||
cylE | F: TGACATTTACAAGTGACGAAG | 248 |
R: TTGCCAGGAGGAGAATAGGA | ||
bca | F:TAACAGTTATGATACTTCACAGAC | 320 |
R:ACGACTTTCTTCCGTCCACTTAGG | ||
cfb | F: GCTGTTTGAAGTGCTGCTTG | 288 |
R: GACTTCATTGCGTGCCAAC | ||
lmb | F: AGTCAGCAAACCCCAAACAG | 397 |
R: GCTTCCTCACCAGCTAAAACG | ||
hylB | F: CACCAATCCCCACTCTACTA | 346 |
R: TGTGTCAAACCATCTATCAG | ||
fbsA | F:AGAGCCAAGTAGGTCAACTTATAG | 290 |
R: TTCATTGCGTCTCAAACCG | ||
bibA | F:AACCAGAAGCCAAGCCAGCAACC | 127 |
R: AGTGGACTTGCGGCTTCACCC | ||
cspA | F: GGTCGCGATAGAGTTTCTTCCGC | 104 |
R: AACGCCTGGGGCTGATTTGGC | ||
sip | F: TCTCTCAATACAATTTCGGAAG | 867 |
R: GTGGTCATAGTGGTTGGCAGT | ||
iagA | F: CGGGATTGATCTAAGTCGCT | 459 |
R: CCATCAACATCAGTCGCTAA | ||
ermA | F: TCTAAAAAGCATGTAAAAGAA | 645 |
R: CTTCGATAGTTTATTAATATTAGT | ||
ermB | F: ATTGGAACAGGTAAAGGGC | 442 |
R: GAACATCTGTGGTATGGCG | ||
ermC | F: TCAAAACATAATATAGATAAA | 642 |
R: GCTAATATTGTTTAAATCGTCAAT | ||
sul1 | F: CATTGCCTGGTTGCTTCAT | 238 |
R: ATCCGACTCGCAGCATTT | ||
sul2 | F: CATCATTTTCGGCATCGTC | 793 |
R: TCTTGCGGTTTCTTTCAGC | ||
sul3 | F: AGATGTGATTGATTTGGGAGC | 443 |
R: TAGTTGTTTCTGGATTAGAGCCT | ||
gyrA | F: GACAAGTGAAATGAAAACGAG | 474 |
R: CGCTCCATTGACTAATAAATTAGG | ||
parC | F: CAAAACATGTCCCTTGAGGA | 568 |
R: CTAGCTTTGGGATGATCAATCAT |
基因名称 Gene name | 引物序列 Primer sequence | 目的片段 Target fragment(bp) |
---|---|---|
GAPDH | F:CGCCATCAGTAGCCCAGT | 219 |
R:CATTCCGTCGCATCCAAA | ||
bibA | F:AACCAGAAGCCAAGCCAGCAACC | 127 |
R:AGTGGACTTGCGGCTTCACCC | ||
cspA | F:AGTTAGGTGCCGTATCTGTAC | 177 |
R:CAAGGGCTGATGACGTGTTG | ||
fbsA | F:GTATCTCAGGTTACTAATGTAGC | 163 |
R:GTCTCAAACCGCAGCGACT | ||
hylB | F:AAGCCCTTGGTGGTAATCTAG | 178 |
R:GCAACATTCGTATGGTCGATG | ||
iagA | F:GGGTTATTCCTCGAAAGGTTAG | 145 |
R:TCTGTCTCTTCACGACTCGG | ||
pavA | F:ACTACTCCTATCTGCTCATCC | 134 |
R:GAATGGTTTCAATCACTGCACC | ||
cfb | F:GACTTCATTGCGTGCCAACC | 166 |
R:CAGCTTAGTTATCCCAAATCCC | ||
sip | F:AGCAAGCTGTTAGTCAAGCAG | 160 |
R:GTTTCAGCGGCAACAGAAGC |
Table 2 Sequence of fluorescent quantitative primers for virulence gene of Streptococcus agalactiae
基因名称 Gene name | 引物序列 Primer sequence | 目的片段 Target fragment(bp) |
---|---|---|
GAPDH | F:CGCCATCAGTAGCCCAGT | 219 |
R:CATTCCGTCGCATCCAAA | ||
bibA | F:AACCAGAAGCCAAGCCAGCAACC | 127 |
R:AGTGGACTTGCGGCTTCACCC | ||
cspA | F:AGTTAGGTGCCGTATCTGTAC | 177 |
R:CAAGGGCTGATGACGTGTTG | ||
fbsA | F:GTATCTCAGGTTACTAATGTAGC | 163 |
R:GTCTCAAACCGCAGCGACT | ||
hylB | F:AAGCCCTTGGTGGTAATCTAG | 178 |
R:GCAACATTCGTATGGTCGATG | ||
iagA | F:GGGTTATTCCTCGAAAGGTTAG | 145 |
R:TCTGTCTCTTCACGACTCGG | ||
pavA | F:ACTACTCCTATCTGCTCATCC | 134 |
R:GAATGGTTTCAATCACTGCACC | ||
cfb | F:GACTTCATTGCGTGCCAACC | 166 |
R:CAGCTTAGTTATCCCAAATCCC | ||
sip | F:AGCAAGCTGTTAGTCAAGCAG | 160 |
R:GTTTCAGCGGCAACAGAAGC |
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