Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (2): 425-432.DOI: 10.6048/j.issn.1001-4330.2022.02.020
• Horticultural Special Local Products·Forestry·Soil Fertilizer· Water Saving Irrigation·Agroecological Environment • Previous Articles Next Articles
ZHAO Zhiqiang(), ZHANG Luhui, ZHAO Quanxin, GUO Qingyuan()
Received:
2021-01-21
Online:
2022-02-20
Published:
2022-03-22
Correspondence author:
GUO Qingyuan
Supported by:
通讯作者:
郭庆元
作者简介:
赵志强(1994-),男,新疆伊宁人,硕士研究生,研究方向为作物病害及其防治,(E-mail) 1758385115@qq.com
基金资助:
CLC Number:
ZHAO Zhiqiang, ZHANG Luhui, ZHAO Quanxin, GUO Qingyuan. Identification of Pathogen Causing Sugar Beet Gibellulopsis Yellow Wilt Based on Multiple Gene Sequence Analysis[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 425-432.
赵志强, 张卢慧, 赵全新, 郭庆元. 新疆发现甜菜新病害甜菜黄萎病[J]. 新疆农业科学, 2022, 59(2): 425-432.
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位点名称 Clone name | 引物名称 Primer name | 引物类型 Primer type | 引物序列(5'-3') Primer Sequence | 引物长度 The length of primer (bp) | 文献来源 Reference |
---|---|---|---|---|---|
ITS | ITS1 | Forward | TCCGTAGGTGAACCTGCGG | 19 | [ |
ITS4 | Reverse | TCCTCCGCTTATTGATATGC | 20 | ||
LSU | LROR | Forward | ACCCGCTGAACTTAAGC | 17 | [ |
LR5 | Reverse | TCCTGAGGGAAACTTCG | 17 | ||
ACT | ACT 512F | Forward | ATGTGCAAGGCCGGTTTCGC | 20 | [ |
ACT 783R | Reverse | TACGAGTCCTTCTGGCCCAT | 20 |
Table 1 Primer sequence for PCR amplification
位点名称 Clone name | 引物名称 Primer name | 引物类型 Primer type | 引物序列(5'-3') Primer Sequence | 引物长度 The length of primer (bp) | 文献来源 Reference |
---|---|---|---|---|---|
ITS | ITS1 | Forward | TCCGTAGGTGAACCTGCGG | 19 | [ |
ITS4 | Reverse | TCCTCCGCTTATTGATATGC | 20 | ||
LSU | LROR | Forward | ACCCGCTGAACTTAAGC | 17 | [ |
LR5 | Reverse | TCCTGAGGGAAACTTCG | 17 | ||
ACT | ACT 512F | Forward | ATGTGCAAGGCCGGTTTCGC | 20 | [ |
ACT 783R | Reverse | TACGAGTCCTTCTGGCCCAT | 20 |
Fig.3 Morphology characteristics of the pathogen causing Gibellulopsis yellow wilt on sugar beet Note:A.Colony on PDA; B.Conidiophore ; C.Condium; D.Chlamydospore.Scale bars:B,C,D=20 μm
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