Effects of Sheep Myostatin Propeptide on Myoblast Differentiation

doi:10.6048/j.issn.1001-4330.2021.03.022

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (3): 581-588.DOI: 10.6048/j.issn.1001-4330.2021.03.022

• Horticultural Special Local Products·Plant Protection·Animal Husbandry Veterinarian·Microbes·Agroecological Environment • Previous Articles

Effects of Sheep Myostatin Propeptide on Myoblast Differentiation

DU Wei, XIA Jun, LIU Mingjun, ZHANG Yang

Research Insititute of Animal Sciences,Xinjiang Academy of Animal Sciences,Urumqi 830000,China

Received:2020-04-24 Online:2021-03-20 Published:2021-03-30
Correspondence author: ZHANG Yang(1963-),male,native place:Hebei,researcher,research field:Animal Genetics and breeding,(E-mail)zhyang@mail.xj.cninfo.net
Supported by:
Outstanding Young Science and Technology Personnel Training Program "Construction of Myostatin mature peptide nucleic acid vaccine and its effect on skeletal muscle growth" the Tianshan Elite Project of Xinjiang Uygur Autonomous Region and Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region "The Integrated Technology of Health Breeding of Brown Cattle in Xinjiang"(XY2019115)

绵羊Myostatin前肽对成肌细胞的分化作用

杜玮, 夏俊, 刘明军, 张杨

新疆畜牧科学院畜牧研究所,乌鲁木齐 830000

通讯作者: 张杨(1962-),男,河北人,研究员,研究方向为遗传育种和畜牧业产业化,(E-mail)zhyang@mail.xj.cninfo.net
作者简介:杜玮(1981-),女,新疆人,副研究员,研究方向为动物遗传育种,(E-mail)12680624@qq.com
基金资助:
杰出青年科技人才培养项目“Myostatin成熟肽核酸疫苗的构建及对骨骼肌生长的影响”;自治区天山英才工程;自治区公益性科研院所基本科研业务经费“新疆褐牛健康养殖技术集成研究”(XY2019115)

Abstract

Abstract: 【Objective】 To study the effect of sheep myostatin propeptide on myoblast differentiation.【Method】 Myostatin cDNA Lentivirus vector plasmid was constructed and expressed in C2C12 cell and the ability of myostatin prepeptide to infect C2C12 cells was studied.【Result】 The LV-CMV -GFP infection group showed obvious long strips of myotubes, while LV-CMV-MSTN-flag-GFP group saw only a small amount of myotubes formed. To further determine the differentiation of myoblasts, myotube fusion rate was determined. The myotube fusion rates of were 5.53%, 6.62% and 9.38% respectively in the untreated group, the control group and the test group. The fusion rate of myotubes in the test group was significantly higher than that in the control group and the untreated group (P<0.05).【Conclusion】 The overexpression of Myostatin pro-peptide gene inhibited the expression of Myostatin gene, thus affecting the formation of myotubes and inhibiting the process of differentiation.

Key words: myostatin propeptide; C2C12 Cells; myotube fusion rate

摘要： 【目的】 研究绵羊Myostatin前肽对C2C12细胞分化的影响。【方法】 构建并包装Myostatin前肽基因过表达慢病毒质粒,包装慢病毒,分别用LV-CMV-MSTN-flag-GFP和LV-CMV -GFP慢病毒感染C2C12细胞,检测重组慢病毒对C2C12细胞的感染能力。【结果】 LV-CMV -GFP感染组可见明显的长条状肌管,LV-CMV-MSTN-flag-GFP组只见少量肌管的形成,肌管融合率的测定。未处理组,对照组,试验组肌管融合率分别为5.53%、6.62%、9.38%,试验组即转染Myostatin前肽的肌管融合率显著高于对照组和未处理组(P＜0.05)。【结论】 Myostatin前肽基因的过表达抑制了Myostatin基因的表达,影响肌管的形成,抑制了分化的进程。

关键词: Myostatin前肽, C2C12细胞, 肌管融合率

CLC Number:

S826
S188

DU Wei, XIA Jun, LIU Mingjun, ZHANG Yang. Effects of Sheep Myostatin Propeptide on Myoblast Differentiation[J]. Xinjiang Agricultural Sciences, 2021, 58(3): 581-588.

杜玮, 夏俊, 刘明军, 张杨. 绵羊Myostatin前肽对成肌细胞的分化作用[J]. 新疆农业科学, 2021, 58(3): 581-588.

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Effects of Sheep Myostatin Propeptide on Myoblast Differentiation

绵羊Myostatin前肽对成肌细胞的分化作用

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