Xinjiang Agricultural Sciences

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (1): 143-150.DOI: 10.6048/j.issn.1001-4330.2021.01.017

• Plant Protection ∙ Soil Fertilizer ∙ Storage and Preservation Processing ∙ Agricultural Water and Soil Engineering ∙ Microbes ∙ Agricultural Product Analysis and Detection • Previous Articles     Next Articles

Analysis of Culture Characteristics and Genetic Diversity of Wild Pleurotus Ostreatus Strains

Nurziya Yalimaimaiti1, JIA Wenjie1, HAO Jingzhe1, LUO Ying1, JIA Peisong1, WEI Peng1, Wemqiemu Abuliz2   

  1. 1. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China, MOARA, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
    2. Altay City Basket Engineering Office, Altay Xinjiang 836500, China
  • Received:2020-06-23 Online:2021-01-20 Published:2021-01-20
  • Correspondence author: JIA Wenjie(1969-), Agronomist, Research area: edible fungus research, (E-mail)1612367472@qq.com
  • Supported by:
    Special Training Program of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2019D03020)and The Earmarked Fund for Modern Agriculture Research System(CARS-20)

野生平菇菌株的培养特征及遗传多样性分析

努尔孜亚·亚力买买提1, 贾文捷1, 郝敬喆1, 罗影1, 贾培松1, 魏鹏1, 温切木·阿布列孜2   

  1. 1.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室,乌鲁木齐 830091;
    2.阿勒泰市菜篮子工程办公室,新疆阿勒泰 836500
  • 通讯作者: 贾文捷(1969-),男,河北人,农艺师,研究方向为食用菌栽培,(E-mail)1612367472@qq.com
  • 作者简介:努尔孜亚·亚力买买提(1977-),女,新疆人,副研究员,研究方向为食用菌资源,(E-mail)nrzy729@163.com
  • 基金资助:
    自治区自然科学基金计划特培项目(2019D03020); 现代农业产业技术体系建设专项资金( CARS－20)

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Abstract: 【Objective】 To evaluate the diversity level of the tested strains by biological culture characteristics and genetic diversity analysis of Pleurotus ostreatus strains from different sources in the hope of providing creative materials and basic data support for the enrichment and development of the germplasm resources of pleurotus ostreatus and the breeding of new varieties.【Methods】 Traditional biological methods and ISSR marker technique were used to carry out the genetic diversity analysis of 15 wild pleurotus ostreatus strains.【Results】 In the aspect of mycelia culture characteristics, the color of each strain was basically white, and there was no obvious difference between strains; There were significant differences in many aspects including colony morphology and mycelium growth, among which the strain 04131 from Yunnan Province had the fastest growth rate, while the strain 00440 from New Zealand had the slowest growth rate; 5 ISSR primers amplified a total of 62 clear DNA bands, including 59 polymorphic bands with a polymorphism ratio of 95.16%; The results of cluster analysis showed that the genetic similarity coefficient of each strain ranged from 0.58 to 0.84, and the 15 tested strains were divided into 5 groups at the genetic similarity coefficient of 0.60, showing obvious regional characteristics. 【Conclusion】 There are significant differences in colony morphology and mycelium growth of 15 pleurotus ostreatus strains from different sources. The genetic diversity is related to its geographical origin, but not to its biological characters. The tested strains have rich genetic diversity and good domestication breeding potential.

Key words: Pleurotus ostreatus; characteristics of mycelium culture; molecular marker; genetic distance

摘要： 【目的】 通过对不同来源的野生平菇菌株进行生物学培养特征测定和遗传多样性分析,评价供试菌株的多样性水平,为丰富和开发平菇种质资源及新品种选育提供创制材料及基础数据支撑。【方法】 利用生物学方法和ISSR标记技术对15株野生平菇菌株进行遗传多样性分析。【结果】 在菌丝培养特征方面,各平菇菌株菌丝颜色基本为白色,菌株间无明显差异;而菌落形态、菌丝长势等方面存在明显差异,其中来自中国云南菌株04131菌丝长速最快,来自新西兰的菌株00440长速最慢;5条ISSR引物共扩增出62条清晰的DNA条带,其中多态性条带59条,多态比率为 95.16%;各菌株间遗传相似系数范围在 0.58~0.84,在遗传相似系数0.60水平上15个供试菌株分为5个类群,表现出较明显的地域性。【结论】 15个不同来源的野生平菇菌株菌落形态、菌丝长势等方面存在较大差异,平菇菌株的遗传多样性与地理来源较为相关,而与生物学性状无明显相关,供试菌株遗传多样性丰富,具有较好的驯化育种潜力。

关键词: 野生平菇, 菌丝培养特征, 分子标记, 遗传距离

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