Association Analysis of Verticillum Wilt Resistance Trait Using SSR Marker in Gossypium hirsutum L.

doi:10.6048/j.issn.1001-4330.2018.09.001

Abstract

Abstract: 【Objective】 This project aims to detect the genotypes of 186 upland cotton germplasm resources by 140 SSR (simple sequence repeat) markers distributed on 26 cotton chromosomes.【Method】Genome-wide association analysis between SSR loci and verticillum wilt resistance trait was performed using GLM (General line model P<0.001) and MLM (Mixed linear model P<0.01).【Result】The main results showed that: (1) The 186 cultivars could be divided into two subgroups: one containing 96 germplasm resources and the other containing 90 germplasm resources; (2) 140 pairs of SSR primers were used, and 355 alleles were detected. The average variation of each pair of primers was 2.54, the average value was 0.759, and the range of primer polymorphism information ranged from 0.503 to 0.995; (3) Correlation analysis showed that there were 22 SSR loci associated with Verticillium wilt resistance traits in cotton under two models, among which 2 loci, NAU998 and CGR5258, could be detected simultaneously in more than two environments. The interpretation rates of the observed phenotypic variation of them were 5.53% and 12.07%, respectively.【Conclusion】The results of this study provide reference information for the utilization of cotton breeding materials for resistance to verticillium wilt and molecular marker-assisted selection.

Key words: Gossypium hirsutum L.; verticillum wilt; association analysis; molecular marker ; ;

摘要： 【目的】对186份陆地棉种质资源的抗黄萎病性状进行关联分析,为抗黄萎病棉花育种材料的利用、分子标记辅助选择提供参考。【方法】采用分布于26条染色体上的140个SSR(Simple sequence repeat)标记,采用GLM(General line model)(P<0.001)模型和 MLM(Mixed linear model)(P<0.01)模型,检测186份陆地棉种质资源的基因型。【结果】(1)2个亚群一个含有96份种质资源,另一个含有90份种质资源;(2)平均每对引物的等位变异为2.54,平均值为0.76,引物多态性信息含量变化范围:0.50~0.99;(3)与棉花抗黄萎病性状相关的SSR位点22个中,能同时在两个以上环境出现的位点有2个,为NAU998和CGR5258,表型变异解释率分别为5.53%和12.07%。【结论】该群体结构,可以分为2个亚群,使用140对 SSR引物做分子标记,共检测出355个等位变异,存在于两个模型下且与棉花抗黄萎病性状相关的SSR位点有22个。

关键词: 陆地棉, 黄萎病, 关联分析, 分子标记

CLC Number:

S562
S188

WANG Long, LI Li-bei, MA Qi-feng, GENG Hong-wei, CHEN Quan-jia, QU Yan-ying, FAN Shu-li. Association Analysis of Verticillum Wilt Resistance Trait Using SSR Marker in Gossypium hirsutum L.[J]. Xinjiang Agricultural Sciences, 2018, 55(9): 1569-1578.

王龙, 李黎贝, 马启峰, 耿洪伟, 陈全家, 曲延英, 范术丽. 陆地棉抗黄萎病性状与SSR标记的关联分析[J]. 新疆农业科学, 2018, 55(9): 1569-1578.

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