Cloning and Bioinformatics Analysis of Salt-Tolerance of a PsaH Gene from Suaeda salsa

doi:10.6048/j.issn.1001-4330.2018.07.005

Abstract

Abstract: 【Objective】 A novel PsaH gene was cloned from suaeda salsa,which was a protein subunit of photosystem I.【Method】This project aimed to study the structure and function of PsaH gene, the full-length cDNA sequence of PsaH gene was obtained from Suaeda salsa salt-stress cDNA library through sequencing, and it was validated by bioinformatics analysis, and named SsPsaH (GeneBank Accession Number:KC4048847).【Result】The full-length of SsPsaH gene was 770 bp, containing 438 bp open reda frame (ORF), and encoding 145 amino acids residues. Based on the predicted analysis of bioinformatics, the SsPsaH was located in chloroplast membrane with transmembrane region, and it was a hydrophilic and unstable protein without signal peptide. Its secondary structure elements were mainly random coil. The SsPsaH gene was highly conservative in different plant species. Phylogenetic tree analysis showed that the protein was closely related to the PsaH protein of Spinacia oleracea. The results of RT-PCR indicated that SsPsaH gene was expressed in root, stem and leaf, but it was higher in stem and leaf than in root. This study has provided a basis for the further study on the salt-tolerance function of the gene and its role in the mechanism of salt tolerance in halophytes, and provided a candidate gene for improving the molecular breeding of salt-tolerance in crops.【Conclusion】SsPsaH gene is a protein subunit H of photosystem I. This study would lay the foundation for future studying on the gene function and the role in salt-tolerance of Suaeda salsa.

Key words: Suaeda salsa; photosystem I reaction center subunit H; bioinformatics

摘要： 【目的】PsaH基因是编码光合反应光系统I(PSI)复合蛋白H亚基的基因。为深入开展该基因的耐盐功能研究及其在盐生植物耐盐机制中的作用提供了基础,并为改良作物耐盐性分子育种提供候选基因。【方法】研究从盐地碱蓬(Suaeda salsa)盐胁迫文库中筛选出一个与耐盐相关的PsaH基因,经测序获得全长cDNA序列,命名为SsPsaH(GeneBank Accession Number:KC4048847),对该基因进行生物信息学分析进行结构功能预测。【结果】该基因全长770 bp,开放阅读框(ORF)为438 bp,编码145个氨基酸;该基因所编码的蛋白定位于叶绿体膜系统,无信号肽,含有一个跨膜结构的亲水性不稳定蛋白,二级结构多为无规则卷曲。同时,该基因在不同物种间具有较强的保守性,含有4个高度保守的结构域,系统进化树分析表明,其与菠菜亲缘关系最近。RT-PCR分析表明,SsPsaH基因在根、茎、叶中均有表达,但在茎和叶中表达量高于根。【结论】SsPsaH基因是编码光合反应光系统I(PSI)复合蛋白H亚基的基因。本研究为以后深入开展该基因的耐盐功能研究及其在盐生植物耐盐机制中的作用提供了理论基础。

关键词: 盐地碱蓬, SsPsaH基因, 生物信息学

CLC Number:

S188

HAO Xiao-yan, LI Jian-ping, CHANG Xiao-chun, Zumuremu, GAO Sheng-qi, CHEN Guo, HUANG Quan-sheng. Cloning and Bioinformatics Analysis of Salt-Tolerance of a PsaH Gene from Suaeda salsa[J]. Xinjiang Agricultural Sciences, 2018, 55(7): 1209-1217.

郝晓燕, 李建平, 常晓春, 足木热木, 高升旗, 陈果, 黄全生. 盐地碱蓬PsaH基因的克隆及生物信息学分析[J]. 新疆农业科学, 2018, 55(7): 1209-1217.

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