关键词: 棉花; SSR分子标记; DNA指纹图谱; 纯度

Abstract: 【Objective】 This project aims to construct fingerprinting map and analyze the purity of four main cotton cultivars in Xinjiang in the hope of providing a basis for molecular identification of cotton varieties.【Method】Typically representative early-mature cotton varieties in northern Xinjiang were used as materials, 25 pairs of primers with high polymorphism, good stability selected from 1,000 pairs of SSR primers as the main extension backbone varieties to construct fingerprinting map, and 10 primers were selected to analyze the purity of 4 varieties of cotton.【Result】The number of alleles detected was 70 and the number of alleles detected by each marker ranged from 2 to 7, with an average of 2.8; The polymorphism information content ranged from 0.169,0 to 0.872,9, with an average of 0.688,3; 4 specific primers could completely differentiate the 4 cotton varieties from each other and their construct fingerprinting map. 10 pairs of primers were used to detect the purity of 4 varieties, and the results showed that the variety with highest purity was Xi 62, with the purity of 99.5% and the variety with lowest purity was Xinluzao 6, whose purity was 92.0%.【Conclusion】The fingerprint and the identification purity of the four cotton varieties were constructed by the SSR molecular marker technique, and the theoretical basis for the construction and the purity identification of the fingerprint of the subsequent main varieties was established.

Key words: cotton; SSR molecular markers; DNA fingerprinting map; purity