石榴果实表面真菌的分离鉴定及致病性测定

doi:10.6048/j.issn.1001-4330.2024.01.016

新疆农业科学 ›› 2024, Vol. 61 ›› Issue (1): 148-155.DOI: 10.6048/j.issn.1001-4330.2024.01.016

• 农产品分析检测·林业·农业信息·植物保护 • 上一篇下一篇

石榴果实表面真菌的分离鉴定及致病性测定

于吕健¹^,², 阳瑾¹^,², 丁宇¹^,², 李晓曼³, 刘峰娟², 范盈盈², 梁红玉³, 焦子伟¹(), 王成²()

1.伊犁师范大学生物与地理科学学院,新疆伊宁 835000
2.新疆农业科学院农业质量标准与检测技术研究所/农业农村部荒漠绿洲生态区特色农产品功能营养与健康重点实验室(部省共建)/农业农村部农产品质量安全风险评估实验室(乌鲁木齐)/新疆农产品质量安全实验室,乌鲁木齐 830091
3.新疆农业大学食品科学与药学学院,乌鲁木齐 830052

收稿日期:2023-05-11 出版日期:2024-01-20 发布日期:2024-02-21
通信作者: 焦子伟(1973-),男,河南人,教授,博士,研究方向为微生物生态及绿色有机农业有害生物防控,(E-mail)jiaziwei123@163.com; 王成(1971-),男,陕西人,研究员,博士,研究方向为农产品质量安全,(E-mail)wangchengxi321@sina.com
作者简介:于吕健(1996-),男,黑龙江人,硕士研究生,研究方向为微生物病害防治,(E-mail)ylj2065@sina.com
基金资助:
新疆维吾尔自治区重大科技专项计划项目(2021A02002-4);伊犁师范大学“学实高层次人才岗位”项目(YLSDXSDTR22001)

Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit

YU Lyujian¹^,², YANG Jin¹^,², DING Yu¹^,², LI Xiaoman³, LIU Fengjuan², FAN Yingying², LIANG Hongyu³, JIAO Ziwei¹(), WANG Cheng²()

1. College of Biological and Geographical Sciences,Yili Normal University,Yining Xinjiang 835000,China
2. Institute of Quality Standards & Testing Technology for Agro-Products,Xinjiang Academy of Agricultural Sciences / Key Laboratory of Functional Nutrition and Health of Characteristic Agricultural Products in Desert Oasis Ecological Region(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs/ Laboratory of Quality and Safety Risk Assessment for Agro-Products(Urumqi),Ministry of Agriculture and Rural Affairs / Key Laboratory of Agro-products Quality and Safety of Xinjiang,Urumqi 830091,China
3. College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China

Received:2023-05-11 Online:2024-01-20 Published:2024-02-21
Correspondence author: JIAO Ziwei(1973-),male,born in Henan Province,professor,PhD,research direction:microbial ecology and pest control in green organic agriculture,(E-mail)jiaziwei123@163.com; WANG Cheng(1971-),male,born in Shanxi,researcher,PhD,research direction:safety and quality of agricultural products,(E-mail)wangchengxj321@sina.com
Supported by:
Major S & T Research project of Xinjiang Uygur Autonomous Region(2021A02002-4);Yili Normal University High-level Talent Program(YLSDXSDTR22001)

摘要/Abstract

摘要：

【目的】研究新疆喀什石榴果实表面真菌的种类,并测试其对石榴果实的致病能力。【方法】分离纯化石榴果实表面真菌,通过形态学和分子生物学对真菌进行鉴定分类,并通过刺伤接种试验测定真菌对石榴果实的致病性,为新疆石榴果实真菌病害的预防与治理提供数据参考。【结果】分离鉴定了7株不同的石榴表面真菌,其中4株可明显致石榴果实腐烂:琉球曲霉(Aspergillus luchuensis)和意大利青霉(Penicillium italicum)对石榴果皮和籽粒均表现出较高的侵染能力,水葫芦链格孢(Alternaria eichhorniae)仅对籽粒果肉具有较高侵染能力,阿氏青霉(Penicillium adametzioide)对石榴果皮和籽粒侵染能力较弱。红色篮状菌(Talaromyes rubrifaciens)、南极枝孢菌(Cladosporium antarcticum)和煤油霉菌(Amorphotheca resinae)未表现出对果皮和籽粒明显的致病能力。【结论】新疆南疆生态条件下石榴果实主要致病菌较其他适生地存在一定区别。

关键词: 石榴; 微生物; 真菌病害; 分离鉴定; 致病性

Abstract:

【Objective】 To preliminarily explore the fungal species on the surface of Kashgar pomegranate fruits and test their pathogenic ability.This finding has provided data reference for the prevention and control of fungal diseases in pomegranate fruit in Xinjiang. 【Methods】 The fungi on the surface of pomegranate fruit were isolated,purified,identified and classified by morphology and molecular biology,and then the pathogenicity of fungi to pomegranate fruit was determined by puncture inoculation test. 【Results】 Seven different fungi were isolated and identified,among which,the following four could obviously cause pomegranate fruit rot. Both of Aspergillus luchuensis and Penicillium italicum showed higher infection ability to pomegranate peel and grain; Alternaria eichhorniae only had higher infection ability to pomegranate pulp; Penicillium adametzioid had weaker infection ability to pomegranate peel and grain. In addition,Talaromyes rubrifaciens,Cladosporium antarctica and Amorphotheca resinae did not show obvious pathogenicity to peel and grain. 【Conclusion】 The main pathogenic bacteria of pomegranate fruit under the ecological conditions in southern Xinjiang are different from those in other suitable growing habitats in China.

Key words: pomegranate; microorganism; fungal diseases; isolation and identification; pathogenicity

中图分类号:

S665.4

于吕健, 阳瑾, 丁宇, 李晓曼, 刘峰娟, 范盈盈, 梁红玉, 焦子伟, 王成. 石榴果实表面真菌的分离鉴定及致病性测定[J]. 新疆农业科学, 2024, 61(1): 148-155.

YU Lyujian, YANG Jin, DING Yu, LI Xiaoman, LIU Fengjuan, FAN Yingying, LIANG Hongyu, JIAO Ziwei, WANG Cheng. Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 148-155.

图/表 3

图1 7种石榴表面分离真菌菌落及孢子形态

Fig 1 Colony and spore morphology of fungi isolated from the surface of 7 pomegranates

图2 7株石榴表面分离真菌rDNA-ITS序列系统发育树

Fig. 2 Phylogenetic tree of rDNA ITS sequence of 7 fungi isolated from pomegranate surface

图3 7株石榴表面分离真菌对石榴损伤部位致腐情况

Fig.3 Decay of pomegranate caused by 7 different fungi isolated from pomegranate surface

参考文献 20

[1]	尹燕雷, 任建辉, 何天明, 等. 新疆的石榴栽培状况[J]. 落叶果树, 2006, 38(6):15-16.
	YIN Yanlei, REN Jianhui, HE Tianming, et al. Cultivation of Pomegranate in Xinjiang[J]. Deciduous Fruits, 2006, 38(6):15-16.
[2]	Viuda-Martos M, Fernández-López J, Pérez-Álvarez J A. Pomegranate and its Many Functional Components as Related to Human Health:A Review[J]. Comprehensive Reviews in Food Science and Food Safety, 2010, 9(6):635-654. DOI URL
[3]	Pooja S, Sarah M C, Farrukh A. Pomegranate for Prevention and Treatment of Cancer:An Update[J]. Molecules, 2017, 22(1):177. DOI URL
[4]	陈芸, 詹建立, 阿依西古丽·外力, 等. 南疆石榴主栽品种调查及果实品质初步鉴定[J]. 黑龙江农业科学, 2016,(1):83-86.
	CHEN Yun, ZHAN Jianli, Ayixiguli Waili, et al. Main Cultivars Investigation and Fruit Quality Identification of Punica granatum L. in South Xinjiang[J]. Heilongjiang Agricultural Sciences, 2016,(1):83-86.
[5]	Munhuweyi K, Lennox C L, Meitz-Hopkins J C, et al. Major diseases of pomegranate(Punica granatum L.),their causes and management—A review[J]. Scientia Horticulturae, 2016, 211:126-139. DOI URL
[6]	Mincuzzi A, Sanzani S M, Paloul, et al. Postharvest Rot of Pomegranate Fruit in Southern Italy:Characterization of the Main Pathogens[J]. Journal of Fungi, 2022, 8(5):475. DOI URL
[7]	郭建伟, 郭娟, 杨建, 等. 石榴病原菌与病害研究综述[J]. 安徽农业科学, 2013, 41(25):10301-10303.
	GUO Jianwei, GUO Juan, YANG Jian, et al. Review of Pomegranate Pathogens and Their Corresponding Disease[J]. Journal of Anhui Agricultural Sciences, 2013, 41(25):10301-10303.
[8]	魏景超遗. 真菌鉴定手册[M]. 上海: 科学技术出版社,1979.
	WEI Jingchaoyi. Manual for Fungal Identification[M]. Shanghai: Science and Technology Press,1979.
[9]	H.L.Barnett, B.B Hunter, 沈崇尧. 半知菌属图解[M]. 北京: 科学出版社,1977.
	Barnett H. L, Hunter B B, SHEN Chongyao. lllustrations of Banzhibacteria. Illustrated Genera of Imperfect Fungi[M]. Beijing: Science Press,1977.
[10]	Guo M J, Wang Q T, Cheng Y H, et al. Identification of Aspergillus tubingensis causing pomegranate fruit rot in China[J]. Australasian Plant Pathology, 2021, 50(2):233-240. DOI
[11]	Yehia H M. Heart rot caused by Aspergillus Niger through splitting in leathery skin of pomegranate fruit[J]. African Journal of Microbiology Research, 2013, 7(9):834-837.
[12]	Thomas T. Fruit rots of pomegranate(cv. wonderful) in Greece[J]. Australasian Plant Pathology, 2014, 43(5):583-588. DOI URL
[13]	Palou L, MA Del Rio. Assessment of fungal pathogens causing postharvest decay of pomegranate in southeast Spain[J]. Acta Horticulturae, 2009,(818):305-312.
[14]	Frisvad J C, Neriman Y, Ulf T, et al. Talaromyces atroroseus,a new species efficiently producing industrially relevant red pigments[J]. PLOS ONE, 2013, 8(12):e84102.
[15]	Mekala Venkatachalam, Hélène Magalon, Laurent Dufossé, et al. Production of pigments from the tropical marine-derived fungi Talaromyces albobiverticillius:New resources for natural red-colored metabolites[J]. Journal of Food Composition and Analysis, 2018, 70:35-48. DOI URL
[16]	Tedford E C, Adaskaveg J E, Ott A J. Impact of Scholar(a new post -harvest fungicide)on the California pomegranates industry[J]. Plant Health Progress, 2005, 10(1):94.
[17]	孙劲, 何子文, 郑晓慧. 凉山州石榴贮藏期病害病原菌的鉴定[C]. 见:王志强,第二届中国石榴博览会暨第七届全国石榴生产与科研研讨会论文集,第二届中国石榴博览会暨第七届全国石榴生产与科研研讨会,安徽怀远, 2017:163-169.
	SUN Jin, HEZiwen,ZHENG Xiaohui. Identification of disease pathogens in Liangshan Prefecture Pomegranate during storage[C]. Proceedings of the 2nd China Pomegranate Expo and the 7th National Pomegranate Production and Scientific Research Symposium,the 2nd China Pomegranate Expo and the 7th National Pomegranate Production and Scientific Research Symposium,Huaiyuan,Anhui, 2017:163-169.
[18]	畅引东, 贾俊, 解宇洁, 等. 石榴果腐病病原的分离鉴定[J]. 山西农业科学, 2020, 48(5):806-811.
	CHANG Yindong, JIA Jun, XIE Yujie, et al. Isolation and Identification of Pathogen of Pomegranate Fruit Rot[J]. Journal of Shanxi Agricultural Sciences, 2020, 48(5):806-811.
[19]	付娟妮, 刘兴华, 蔡福带, 等. 石榴采后腐烂病病原菌的分子鉴定[J]. 园艺学报, 2007, 34(4):877-882.
	FU Juanni, LIU Xinghua, CAI Fudai, et al. Identification of Pathogenic Fungus Causing a Decay of Stored Pomegranate Fruits Using Molecular Biology Technique[J]. Acta Horticulturae Sinica, 2007, 34(4):877-882.
[20]	张润光, 张有林, 赵蕾丹. 石榴贮藏期间褐腐病病原菌的分离、鉴定及不同理化因素对其生长繁殖的影响[J]. 食品工业科技, 2012, 33(22):338-341.
	ZHANG Runguang, ZHANG Youlin, ZHAO Leidan. Separation and identification the pathogens of browning rotten disease of pomegranate during storage and effect of different physical and chemical factors on its reproduction[J]. Science and Technology of Food Industry, 2012, 33(22):338-341.

石榴果实表面真菌的分离鉴定及致病性测定

Isolation,identification and pathogenicity of fungi on the surface of pomegranate fruit

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