新疆农业科学 ›› 2023, Vol. 60 ›› Issue (9): 2109-2119.DOI: 10.6048/j.issn.1001-4330.2023.09.004
• 作物遗传育种·种质资源·分子遗传学·土壤肥料 • 上一篇 下一篇
王辉1(), 郭金成1, 宋佳1, 张庭军2(), 何良荣1()
收稿日期:
2022-11-12
出版日期:
2023-09-20
发布日期:
2023-09-19
通信作者:
何良荣(1970-),女,湖北人,副教授,博士,硕士生导师,研究方向为棉花种质资源,(E-mail)作者简介:
王辉(1993-),男,河南人,硕士研究生,研究方向为作物种质资源,(E-mail)347488940@qq.com
基金资助:
WANG Hui1(), GUO Jincheng1, SONG Jia1, ZHANG Tingjun2(), He Liangrong1()
Received:
2022-11-12
Online:
2023-09-20
Published:
2023-09-19
Correspondence author:
HE Liangrong(1970-), female, Hubei, master, research direction: research and innovation of crop germplasm resources,(E-mail)Supported by:
摘要:
【目的】研究高温胁迫条件下陆地棉GhCIPK6转基因后代幼苗体内光合特性指标、抗性指标、植物激素的变化,为陆地棉响应高温胁迫奠定基础。【方法】以构建GhCIPK6转基因后代超表达、RNAi和对照野生型为材料,采用温室培养的方法,在4、12、24、48 h测定生理生化指标。【结果】通过幼苗叶片比较,高温下超表达叶片已经发黄,出现大量斑点,野生型叶片发黄程度次之,RNAi叶片只轻微发黄;光合特性中,高温胁迫下随着时间延长净光合速率、气孔导度、蒸腾速率、细胞间CO2浓度均呈先下降后上升,超表达材料在光合指标中增幅较慢,RNAi和野生型增幅较快,叶绿素相对含量变化趋势缓慢;在抗性指标中,高温胁迫下,超表达随着时间延长MAD含量逐渐增加,SOD含量呈先增加后降低后上升,POD含量表现出较高水平,RNAi中MAD含量呈先增加后下降,SOD含量呈先上升后下降,POD含量缓慢增加,可溶性蛋白含量和Pro含量在整个时期相对呈现较高的水平;植物激素中,随着时间延长超表达材料IAA含量呈上升趋势,ABA含量较低,JA含量先缓慢增加后下降,SA先增加后下降,RNAi材料中IAA含量逐渐上升,ABA含量呈先增加后下降,JA含量先增加后下降,SA含量先下降后上升后下降,野生型中IAA含量先上升后下降,ABA含量呈先上升再下降后上升,JA含量先增加后下降,SA含量先下降后上升后下降。3种材料抗高温能力由强到弱为RNAi,野生型,超表达。【结论】在高温下可通过提高蒸腾速率来减弱逆境胁迫的危害,提高生长素含量来积极调控植物生长,提高细胞的渗透调节能力来减轻细胞遭受的损伤。
中图分类号:
王辉, 郭金成, 宋佳, 张庭军, 何良荣. 高温胁迫下陆地棉GhCIPK6转基因后代生理生化分析[J]. 新疆农业科学, 2023, 60(9): 2109-2119.
WANG Hui, GUO Jincheng, SONG Jia, ZHANG Tingjun, He Liangrong. Physiological and biochemical analysis of transgenic offspring of upland cotton GhCIPK6 under high temperature Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2109-2119.
图1 陆地棉叶片比较 注:A、B分别代表4 h正长温度和高温幼苗,C代表48 h幼苗叶片
Fig. 1 Comparison of upland cotton leaves Note:AB represent 4h positive growth temperature and high temperature seedlings respectively, and C represents 48h seedling leaves
因子 Factors | 成分 Principal components | |||
---|---|---|---|---|
F1 | F2 | F3 | F4 | |
丙二醛 MDA | -0.324 | 0.207 | 0.726 | -0.41 |
净光合速率 Pn | 0.798 | 0.264 | 0.452 | 0.178 |
气孔导度 Gs | 0.9 | -0.022 | 0.251 | 0.307 |
胞间CO2浓度 Ci | 0.843 | -0.05 | -0.086 | -0.125 |
蒸腾速率Tr | 0.857 | -0.025 | 0.313 | 0.375 |
叶绿素相对值SPAD | 0.816 | 0.074 | 0.057 | -0.465 |
超氧化物歧化酶SOD | 0.573 | 0.559 | -0.318 | 0.276 |
过氧化物酶POD | -0.637 | -0.251 | 0.619 | 0.179 |
可溶性蛋白Souble protein | -0.074 | -0.827 | 0.234 | -0.155 |
脯氨酸Pro | -0.635 | -0.434 | 0.113 | 0.449 |
脱落酸 ABA | 0.481 | -0.566 | -0.374 | -0.136 |
生长素IAA | 0.013 | 0.686 | 0.47 | -0.277 |
茉莉酸JA | -0.604 | 0.591 | -0.47 | -0.116 |
水杨酸SA | -0.476 | 0.666 | 0.08 | 0.333 |
方差贡献率 Variance contribution rate(%) | 40.182 | 21.09 | 14.601 | 8.714 |
累计贡献率 Cumulative contribution rate(%) | 40.182 | 61.272 | 75.873 | 84.587 |
表1 主成分因子载荷矩阵及主成分特征根
Tab.1 Factor loading matrix of main composition and principal component eigenvalues
因子 Factors | 成分 Principal components | |||
---|---|---|---|---|
F1 | F2 | F3 | F4 | |
丙二醛 MDA | -0.324 | 0.207 | 0.726 | -0.41 |
净光合速率 Pn | 0.798 | 0.264 | 0.452 | 0.178 |
气孔导度 Gs | 0.9 | -0.022 | 0.251 | 0.307 |
胞间CO2浓度 Ci | 0.843 | -0.05 | -0.086 | -0.125 |
蒸腾速率Tr | 0.857 | -0.025 | 0.313 | 0.375 |
叶绿素相对值SPAD | 0.816 | 0.074 | 0.057 | -0.465 |
超氧化物歧化酶SOD | 0.573 | 0.559 | -0.318 | 0.276 |
过氧化物酶POD | -0.637 | -0.251 | 0.619 | 0.179 |
可溶性蛋白Souble protein | -0.074 | -0.827 | 0.234 | -0.155 |
脯氨酸Pro | -0.635 | -0.434 | 0.113 | 0.449 |
脱落酸 ABA | 0.481 | -0.566 | -0.374 | -0.136 |
生长素IAA | 0.013 | 0.686 | 0.47 | -0.277 |
茉莉酸JA | -0.604 | 0.591 | -0.47 | -0.116 |
水杨酸SA | -0.476 | 0.666 | 0.08 | 0.333 |
方差贡献率 Variance contribution rate(%) | 40.182 | 21.09 | 14.601 | 8.714 |
累计贡献率 Cumulative contribution rate(%) | 40.182 | 61.272 | 75.873 | 84.587 |
材料 Materials | 时间 Time | F1 | F2 | F3 | F4 | 综合得分 Comprehensive score | 综合排序 Comprehensive ranking |
---|---|---|---|---|---|---|---|
WT | 4 | 2.912 | -0.330 | 0.406 | -1.114 | 0.149 | 3 |
12 | -4.233 | 1.175 | -1.983 | -0.778 | -0.253 | 12 | |
24 | 0.178 | 0.960 | 2.404 | 0.207 | 0.090 | 5 | |
48 | 2.309 | 1.532 | -1.393 | 0.275 | 0.150 | 2 | |
Ri | 4 | 2.837 | -0.822 | -1.246 | -1.425 | 0.092 | 4 |
12 | -2.519 | 1.746 | -0.114 | -1.211 | -0.107 | 10 | |
24 | -1.572 | 1.307 | 2.498 | 0.276 | 0.005 | 6 | |
48 | 2.482 | 2.150 | -0.224 | 1.069 | 0.212 | 1 | |
OE | 4 | 0.808 | -2.588 | 0.587 | -0.524 | -0.025 | 8 |
12 | -2.471 | -2.673 | -0.263 | 0.366 | -0.219 | 11 | |
24 | -0.569 | -1.863 | 0.708 | 0.372 | -0.068 | 9 | |
48 | -0.162 | -0.593 | -1.378 | 2.488 | -0.024 | 7 |
表2 主成分得分及综合评价
Tab.2 Principal scores and comprehensive evaluation results
材料 Materials | 时间 Time | F1 | F2 | F3 | F4 | 综合得分 Comprehensive score | 综合排序 Comprehensive ranking |
---|---|---|---|---|---|---|---|
WT | 4 | 2.912 | -0.330 | 0.406 | -1.114 | 0.149 | 3 |
12 | -4.233 | 1.175 | -1.983 | -0.778 | -0.253 | 12 | |
24 | 0.178 | 0.960 | 2.404 | 0.207 | 0.090 | 5 | |
48 | 2.309 | 1.532 | -1.393 | 0.275 | 0.150 | 2 | |
Ri | 4 | 2.837 | -0.822 | -1.246 | -1.425 | 0.092 | 4 |
12 | -2.519 | 1.746 | -0.114 | -1.211 | -0.107 | 10 | |
24 | -1.572 | 1.307 | 2.498 | 0.276 | 0.005 | 6 | |
48 | 2.482 | 2.150 | -0.224 | 1.069 | 0.212 | 1 | |
OE | 4 | 0.808 | -2.588 | 0.587 | -0.524 | -0.025 | 8 |
12 | -2.471 | -2.673 | -0.263 | 0.366 | -0.219 | 11 | |
24 | -0.569 | -1.863 | 0.708 | 0.372 | -0.068 | 9 | |
48 | -0.162 | -0.593 | -1.378 | 2.488 | -0.024 | 7 |
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