新疆农业科学 ›› 2023, Vol. 60 ›› Issue (7): 1671-1678.DOI: 10.6048/j.issn.1001-4330.2023.07.013

• 园艺特产·贮藏保鲜加工 • 上一篇    下一篇

26个鲜食枣品种SSR指纹图谱构建与遗传多样性分析

张雁飞1,2(), 苏比娜·肖克来提1, 杨磊1, 郝庆1, 靳娟1(), 樊丁宇1()   

  1. 1.新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测实验站,乌鲁木齐 830091
    2.新疆农业大学园艺学院,乌鲁木齐 830052
  • 收稿日期:2022-10-10 出版日期:2023-07-20 发布日期:2023-07-11
  • 通信作者: 靳娟(1990-),女,江苏徐州,助理研究员,研究方向果树品质调控及逆境分子生物学,(E-mail)1475063956@qq.com
    樊丁宇(1983-),男,山西忻州人,研究员,研究方向为果树栽培与生理,(E-mail)fandingyu2413@163.com
  • 作者简介:张雁飞(1997-),女,甘肃靖远人,硕士研究生,研究方向为果树栽培与生理,(E-mail)2453118791@qq.com
  • 基金资助:
    新疆维吾尔自治区科技支疆项目“优良鲜食枣‘京沧1号’引进及配套栽培技术集成示范”(2020E0212);中央财政林业改革发展资金项目“鲜食枣新品种优质高效栽培技术示范与推广”(新[2020]TG17)

SSR fingerprinting and genetic diversity analysis of 26 fresh jujube cultivars

ZHANG Yanfei1,2(), Subina Xaokelaiti1, YANG Lei1, HAO Qing1, JIN Juan1(), FAN Dingyu1()   

  1. 1. Scientific Observing and Experimental Station of Pomology (Xinjiang),Ministry of Agriculture and Rural Affairs of the P.R.C. / Research Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
    2. College of Horticulture, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2022-10-10 Online:2023-07-20 Published:2023-07-11
  • Correspondence author: JIN Juan (1990-), female, assistant researcher in Xuzhou, Jiangsu Province, research direction on fruit tree quality regulation and molecular biology of adversity, (E-mail)1475063956@qq.com
    FAN Dingyu (1983-), male, Xinzhou, Shanxi, associate researcher, research direction is reproductive development and hormone regulation of Chinese jujube, (E-mail)fandingyu2413@163.com
  • Supported by:
    The S & T Assisting Xinjiang Program Project "Excellent Fresh Jujube 'Jingcang 1' Introduction and Supporting Cultivation Technology Integration Demonstration"(2020E0212);The Central Financial Forestry Reform and Development Fund Project "Demonstration and Promotion of High-quality and Efficient Cultivation Technology of New Fresh Jujube Varieties"(Xin[2020]TG17)

摘要:

【目的】研究26份鲜食枣种质资源构建指纹图谱,分析遗传多样性,为鲜食枣品种鉴定和遗传多样性分析提供参考依据。【方法】利用22对SSR引物对26个鲜食枣品种进行分析,采用荧光M13毛细管电泳技术进行多态检测,共扩增出130条等位片段,平均5.909个。【结果】多态信息含量(PIC)变化范围为0.237 7~0.855 6,平均为0.59。22对SSR引物的杂合度介于0.500~1.000,平均为0.927。3对引物BFU0308,BFU1157和BFU0574组合能完全区分26个枣品种。【结论】26个品种遗传相似系数在0.65~1.00,相似系数0.65处可将26个品种分为两大类群。

关键词: 鲜食枣; SSR; 毛细管电泳; 指纹图谱

Abstract:

【Objective】 This experiment aims to construct the fingerprint map on 26 fresh jujube germplasm resources and analyze their genetic diversity in the hope of providing a reference for fresh jujube variety identification and genetic diversity analysis.【Methods】 A total of 26 fresh jujube varieties were analyzed using 22 SSR primers and polymorphic by fluorescent M13 capillary electrophoresis that amplified 130 allelic fragments with an average of 5.909.【Results】 The polymorphic information content (PIC) varied from 0.237,7 to 0.855,6, with an average of 0.59.The heterozygosity for 22 SSR primers ranged from 0.500 to 1.000, with an average of 0.927.The combination of three primer pairs, BFU0308, BFU1157 and BFU0574, could completely distinguish the 26 jujube varieties.【Conclusion】 The genetic similarity coefficient of the 26 varieties is between 0.65 and 1.00, and the 26 varieties can be divided into two major groups at 0.65.

Key words: fresh jujube; SSR; capillary electrophoresis; fingerprint

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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