新疆农业科学 ›› 2022, Vol. 59 ›› Issue (7): 1625-1631.DOI: 10.6048/j.issn.1001-4330.2022.07.008

• 作物遗传育种·耕作栽培·种质资源 • 上一篇    下一篇

紫色甘薯资源的形态学和ISSR荧光标记分析

吴巧玉(), 何天久()   

  1. 贵州省农业科学院生物技术研究所,贵阳 550006
  • 收稿日期:2021-10-07 出版日期:2022-07-20 发布日期:2022-08-04
  • 通讯作者: 何天久
  • 作者简介:吴巧玉(1982-),女,四川仁寿人,助理研究员,硕士,研究方向为薯类作物生理生化与育种,(E-mail) wuqiaoyu1@163.com
  • 基金资助:
    贵州省科技计划(黔科合支撑[2020]1Y093号)

Morphological and ISSR Analysis of Purple Sweet Potato Resources

WU Qiaoyu(), HE Tianjiu()   

  1. Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
  • Received:2021-10-07 Online:2022-07-20 Published:2022-08-04
  • Contact: HE Tianjiu
  • Supported by:
    Science and Technology Program of Guizhou Province (2020-1Y093)

摘要:

【目的】分析紫色甘薯品种的遗传多样性,其遗传差异,为紫色甘薯遗传育种亲本选择提供理论依据。【方法】以引进的10份紫色甘薯为材料,利用形态学性状和ISSR 荧光标记毛细管电泳技术分析品种遗传多样性。【结果】基于20项形态学性状的聚类图,在欧式距离为9.97处,可分为两大类群,第一大类为宁紫1号、山东紫薯、渝紫2号、徐紫8号、越南紫薯 ;第二大类为鄂12、宁紫4号、渝紫3号、渝紫11、南紫018 。紫色甘薯种质资源的观测等位基因为(Na)为2个,平均有效等位基因数(Ne)为1.919 0,期望杂合度(He)为0.237 5,香农指数(I)为0.433 3,在遗传距离为0.96时,10份供试紫色甘薯材料可分为两大类。【结论】部分供试材料的ISSR标记聚类结果与形态学标记在遗传背景和类群划分上具有一致性,但是两种鉴定分类方法的聚类分析结果也存在一定差异。紫色甘薯种质材料遗传多样性丰富,将形态学及ISSR标记结合能有效提高品种间特异性的鉴定,更能客观、准确的确定种质资源的亲缘关系。

关键词: 紫色甘薯, 形态学, 聚类分析, ISSR荧光标记, 遗传多样性

Abstract:

【Objective】 To analyze the genetic diversity of purple sweet potato varieties collected from China, clarify their genetic differences in the hope of providing a theoretical basis for the selection of purple sweet potato genetic breeding parents. 【Methods】 Morphological characters and ISSR fluorescent labeling capillary electrophoresis were carried out to analyze the genetic diversity of varieties of 10 purple sweet potatoes imported from other provinces. 【Results】 The results showed that, based on the cluster map of 20 morphological traits, the tested materials could be divided into two major groups at the Euclidean distance of 9.97. The first major category included Ningzi-1, Shandongzi, Yuzi-2, Xuzi-8, Yuenanzi; the second major category consisted of E-12, Ningzi-4, Yuzi-3, Yuzi-11, Nanzi-018. 6 pairs of primers were used in ISSR molecular marker analysis, the results showed that, the observed alleles (Na) of the purple sweet potato germplasm resources were 2, the average effective allele number (Ne) was 1.9190, and the expected heterozygosity (He) was 0.2375 and Shannon's index (I) was 0.4333. Cluster analysis performed with NTSYSpc 2.1 software demonstrated that when the genetic distance was 0.96, the 10 tested purple sweet potato materials could be divided into two categories. 【Conclusion】 The clustering results of SSR markers and morphological markers of several tested materials were consistent in genetic background and group classification, but the cluster analysis results of the two identification and classification methods also had certain differences. The purple sweet potato resources presented very rich genetic diversity. The combination of morphology and ISSR markers could effectively improve the identification of specific varieties and determine the genetic relationship of germplasm resources objectively and accurately.

Key words: purple sweet potato, morphology, cluster analysis, fluorescent ISSR markers, genetic diversity

中图分类号: 


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

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