枣瘿蚊微卫星标记筛选及微卫星多态性分析

doi:10.6048/j.issn.1001-4330.2020.05.012

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (5): 888-894.DOI: 10.6048/j.issn.1001-4330.2020.05.012

枣瘿蚊微卫星标记筛选及微卫星多态性分析

马光皇^1,2, 康淑媛^1,2, 李莉^1,2, 肖海兵^1,2,3, 熊仁次^2,3, 杨明禄^1,2,3

1.塔里木大学植物科学学院,新疆阿拉尔 843300;
2.南疆农业有害生物综合治理兵团重点实验室,新疆阿拉尔 843300;
3.农业部阿拉尔作物有害生物科学观测实验站,新疆阿拉尔 843300

收稿日期:2019-10-20 发布日期:2020-04-23
通信作者: 杨明禄(1976-),男,副教授,硕士,硕士生导师,研究方向为农业昆虫与害虫防治,(E-mail)ymlzkytd@163.com
作者简介:马光皇(1997-),男,安徽人,硕士研究生,研究方向为农业转化与害虫防治,(E-mail)1615405113@qq.com
基金资助:
新疆生产建设兵团国际科技合作计划(2017BC004);中国农业大学-塔里木大学高校联合基金(2019TC159)

Microsatellite Markers Dressing by Screening and Polymorphism Analysis of Contarinia datifolia

MA Guanghuang^1,2, KANG Shuyuan^1,2, LI Li^1,2, XIAO Haibing^1,2,3, XIONG Renci^2,3, YANG Minglu^1,2,3

1.College of Plant Science, Tarim University,Aral Xinjiang 843300, China;
2.Southern Xinjiang Key Laboratory of IPM of Tarim University, Aral Xinjiang 843300, China;
3.Scientific Observing and Experimental Station of Crop Pests in Aral, Ministry of Agriculture, P. R China, Aral Xinjiang 843300, China

Received:2019-10-20 Published:2020-04-23
Correspondence author: Yang Minglu(1976-),male, research field: Agricultural Entomology and Pest Control,(E-mail)ymlzkytd@163.com
Supported by:
The International Science and Technology Cooperation Program of XinJiang Construction Corps (Grant number 2017BC004)and United fund China Agricultural University and Tarim University(Grant number2019TC159)

摘要/Abstract

摘要： 【目的】研究枣瘿蚊基因遗传变异程度,分析基因多态性。【方法】从枣瘿蚊部分基因组序列中搜索SSR并设计相应引物,挑选引物48对进行有效筛选。枣瘿蚊样本来自新疆阿拉尔市、昆玉市、且末县等地区,以枣瘿蚊DNA为模板进行PCR扩增,筛选特异性微卫星引物;通过琼脂糖凝胶电泳进行定性检测,再以毛细管电泳检测其多态性。【结果】有42对引物能扩增出条带,选择其中20对特异性较好的引物进行多态性分析。观测杂合度(Observed heterozygosity,Ho)、期望杂合度(Expected heterozygosity,He)和多态信息含量(PIC)平均值分别是0.358、0.649和0.635,其中18对引物PIC值＞0.5,具有较高的多态性。【结论】开发的微卫星标记多态性较高,可用于枣瘿蚊种群遗传多样性及遗传结构研究。

关键词: 枣瘿蚊; 微卫星(SSR); 多态性; 遗传多样性

Abstract: 【Objective】 The goal was to study the genetic variability of Dasineura jujubifolia genes and analyze its genetic polymorphisms.【Method】 In this experiment, SSR was searched from genome sequences of Dasineura jujubifolia and corresponding primers were designed, from which 48 pairs of primers were selected for effective screening. The Dasineura jujubifolia samples came from Aral, Kunyu and Qiemo and they were amplified by PCR using Dasineura jujubifolia DNA as template. Qualitative detection was performed by agarose gel electrophoresis, and the polymorphism was detected by capillary electrophoresis.【Result】The results show that:There were 42 pairs of primers that could amplify the bands, and 20 with good specificity of them were selected for polymorphism analysis. The average values of observed heterozygosity (Ho), expected heterozygosity (He) and polymorphic information content(PIC)were 0.358, 0.694 and 0.635. Among them, the PIC of 18 pairs of primers was more than 0.5, which was highly polymorphic.【Conclusion】SSR that we developed have high polymorphisms, which can be used to study the genetic diversity and genetic structure of Dasineura jujubifolia population.

Key words: Dasineura jujubifolia; microsatellties; polymorphism; genetic diversity

中图分类号:

S436.65

马光皇, 康淑媛, 李莉, 肖海兵, 熊仁次, 杨明禄. 枣瘿蚊微卫星标记筛选及微卫星多态性分析[J]. 新疆农业科学, 2020, 57(5): 888-894.

MA Guanghuang, KANG Shuyuan, LI Li, XIAO Haibing, XIONG Renci, YANG Minglu. Microsatellite Markers Dressing by Screening and Polymorphism Analysis of Contarinia datifolia[J]. Xinjiang Agricultural Sciences, 2020, 57(5): 888-894.

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枣瘿蚊微卫星标记筛选及微卫星多态性分析

Microsatellite Markers Dressing by Screening and Polymorphism Analysis of Contarinia datifolia

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