建立矮膜苞芹HPLC指纹图谱

doi:10.6048/j.issn.1001-4330.2019.02.012

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (2): 308-316.DOI: 10.6048/j.issn.1001-4330.2019.02.012

建立矮膜苞芹HPLC指纹图谱

胡江兰¹, 朱金芳^1,2, 帕尔哈提·多力坤¹

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052;
2.中国科学院新疆理化技术研究所,乌鲁木齐 830011

收稿日期:2018-12-11 出版日期:2019-02-20 发布日期:2019-05-22
通信作者: 朱金芳(1976-),女,新疆人,博士,副教授,研究方向为食药用天然产物,(E-mail)zjf7619@126.com
作者简介:胡江兰(1993-),女,新疆人,硕士研究生,研究方向为食药用天然产物,(E-mail)hjl0827@126.com
基金资助:
自治区重大专项“民族药创新与产业化示范”(2016A03005)

Study on HPLC Fingerprint of Hymenoleana nana

HU Jiang-lan¹, ZHU Jin-fang^1,2, Parhat Dolkun¹

1.College of Food Sciences and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China;
2.Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China

Received:2018-12-11 Online:2019-02-20 Published:2019-05-22
Correspondence author: Zhu Jin-fang (1976-), female,native place:Xinjiang,research direction:study of natural products of food and medicine, Ph.D., associate professor, (E-mail)zjf7619@126.com
Supported by:
Special National Project Innovation and Industrialization Demonstration" in Xinjiang Autonomous Region

摘要/Abstract

摘要： 【目的】建立矮膜苞芹药材高效液相指纹图谱,为矮膜苞芹药材的质量控制及开发利用提供科学依据。【方法】采用HPLC法,WondaCract ODS-2 C₁₈色谱柱(4.6 mm ×250 mm,5 μm),流动相乙腈: 0.1% 甲酸水梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长283 nm。测定10批药材的指纹图谱,运用《中药色谱指纹图谱相似度评价系统》和统计软件中聚类分析、主成分分析及偏最小二乘法-判别分析对指纹图谱进行分析。【结果】标定出共有色谱峰13个,10批药材中有7批指纹图谱的相似度 >0.9。【结论】建立了矮膜苞芹指纹图谱共有模式,所建立的方法具有良好的精密度、稳定性和重复性。

关键词: 矮膜苞芹; 高效液相色谱; 指纹图谱

Abstract: 【Objective】 To establish the HPLC Fingerprint of Hymenoleana nana and provide a scientific basis for the quality control, the development and utilization of medicinal plant resources.【Method】HPLC was performed using a WondaCract ODS-2 C₁₈column(4.6 mm×250 mm,5 μm), with of mobile phase acetonitrile: 0.1% formic acid water gradient elution, flow rate 1.0 mL/min, column temperature 30℃, detection wavelength 283 nm. The fingerprints of 10 batches of medicinal materials were determined and the fingerprints were analyzed by cluster analysis, principal component analysis and least squares-discriminant analysis using the Similarity Evaluation System for Chromatographic Fingerprint of TCM and statistical software.【Result】The chromatographic evaluation software calibrated 13 common chromatograms, and the similarity of 7 fingerprints in 10 batches was >0.9. 【Conclusion】 The established method has good precision, stability and repeatability, which lays a foundation for the total quality control of the medicinal materials of Hymenoleana nana.

Key words: Hymenoleana nana; HPLC; fingerprint

中图分类号:

S184

胡江兰, 朱金芳, 帕尔哈提·多力坤. 建立矮膜苞芹HPLC指纹图谱[J]. 新疆农业科学, 2019, 56(2): 308-316.

HU Jiang-lan, ZHU Jin-fang, Parhat Dolkun. Study on HPLC Fingerprint of Hymenoleana nana[J]. Xinjiang Agricultural Sciences, 2019, 56(2): 308-316.

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建立矮膜苞芹HPLC指纹图谱

Study on HPLC Fingerprint of Hymenoleana nana

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