19个自育葡萄品种(系)指纹图谱构建

doi:10.6048/j.issn.1001-4330.2020.12.010

新疆农业科学 ›› 2020, Vol. 57 ›› Issue (12): 2238-2249.DOI: 10.6048/j.issn.1001-4330.2020.12.010

• 园艺特产·贮藏保鲜加工·植物保护 • 上一篇下一篇

19个自育葡萄品种(系)指纹图谱构建

王勇, 孙锋, 苏来曼·艾则孜, 李玉玲, 伍国红, 骆强伟, 郭平峰

新疆维吾尔自治区葡萄瓜果研究所,新疆鄯善 838200

收稿日期:2019-07-15 出版日期:2020-12-20 发布日期:2020-12-24
通信作者: 骆强伟(1960-),男,陕西长安人,研究员,研究方向为葡萄资源、育种及栽培,(E-mail)luoqwtq@sohu.com
作者简介:王勇(1982-),男,河南方城人,副研究员,硕士,研究方向为葡萄育种,(E-mail)wangyong3711100@163.com
基金资助:
现代农业产业技术体系建设专项基金(CARS-29-yc-7);新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2019066);新疆维吾尔自治区重点研发任务专项(2016B01005-4)

Construction of the Fingerprints of 19 Self-bred Grape (Vitis vinfera) Varieties (Lines)

WANG Yong, SUN Feng, Sulaiman Aizezi, LI Yuling, WU Guohong, LUO Qiangwei, GUO Pingfeng

Research Institute of Grapes and Melons of Xinjiang Uygur Autonomous Region, Shanshan Xinjiang 838200, China

Received:2019-07-15 Online:2020-12-20 Published:2020-12-24
Correspondence author: LUO Qiangwei(1960-), male, Chang'an, Shaanxi, Fellow, Undergraduate,grape resources, breeding research and cultivation techniques promotion,(E-mail)luoqwtq@sohu.com.
Supported by:
Supported by China Agriculture Research System (CARS-29-yc-7)and Supported by Basic Scientific R &D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region of China(KY2019066)

摘要/Abstract

摘要： 【目的】 利用SSR标记技术开展19个葡萄品种(系)的指纹图谱构建,为种苗认证、品种登记和植物新品种保护提供依据。【方法】 以19个葡萄品种(系)为试材,利用16对SSR标记荧光引物,对其进行PCR扩增和毛细管电泳,获得指纹条带,构建指纹图谱,并筛选可以将19个品种(系)区分的引物组合,绘制种质分子身份证数字码。【结果】 从16对SSR标记引物选择了VRZAG67、VVS2、VVMD7、VCHR4a、VVMD5等5对多态性信息量相对较高的SSR标记,完成了品种(系)的指纹图谱构建,并对其进行了赋值编码,形成了身份识别码。【结论】 构建的指纹图谱和分子身份证可以鉴定和区别19个品种(系),但由于葡萄种质较多,如果继续区别更多的品种,需要增加的 SSR 引物数量。

关键词: 葡萄品种; SSR标记; 指纹图谱

Abstract: 【Objective】 To construct the fingerprints of 19 grape varieties (lines) by SSR technology in the hope of providing basis for seedling certification, variety registration and new plant variety protection. 【Method】 In this project, 19 grape varieties (lines) were used as test materials, 16 pairs of SSR labeled silver primers were used for PCR amplification and capillary electrophoresis to obtain the fingerprint bands, construct the fingerprint map, and select the primer combinations that could distinguish the 19 varieties (lines), encode the alleles and establish the germplasm molecular identity card. 【Result】 In this study, VRZAG67, VVS2, VVMD7, VCHR4a and VVMD5 were selected from 16 pairs of SSR markers, and 5 pairs of SSR markers with relatively high amount of polymorphism information were constructed. The fingerprints of varieties (lines) were constructed, and their assigned values were coded to form an identification code. 【Conclusion】 The fingerprints and molecular ID cards constructed can identify and distinguish 19 varieties (lines), but because there are many grape germplasms, if we continue to distinguish more varieties, we needed to increase the number of SSR primers.

Key words: grape (Vitis vinfera); SSR marker; fingerprint

中图分类号:

S663.1
S188

王勇, 孙锋, 苏来曼·艾则孜, 李玉玲, 伍国红, 骆强伟, 郭平峰. 19个自育葡萄品种(系)指纹图谱构建[J]. 新疆农业科学, 2020, 57(12): 2238-2249.

WANG Yong, SUN Feng, Sulaiman Aizezi, LI Yuling, WU Guohong, LUO Qiangwei, GUO Pingfeng. Construction of the Fingerprints of 19 Self-bred Grape (Vitis vinfera) Varieties (Lines)[J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2238-2249.

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19个自育葡萄品种(系)指纹图谱构建

Construction of the Fingerprints of 19 Self-bred Grape (Vitis vinfera) Varieties (Lines)

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