新疆农业科学 ›› 2023, Vol. 60 ›› Issue (4): 889-896.DOI: 10.6048/j.issn.1001-4330.2023.04.013
史智勇1(), 艾沙江·买买提2, 仙米斯娅·塔依甫3, 曹艺洁1, 玉苏甫·阿不力提甫1()
收稿日期:
2022-09-10
出版日期:
2023-04-20
发布日期:
2023-05-06
通信作者:
玉苏甫·阿不力提甫(1969-),男,新疆乌鲁木齐人,副教授,博士,研究方向为果树栽培与生理,(E-mail)作者简介:
史智勇(1998-),男,河南郑州人,硕士研究生,研究方向为果树生理及品质调控,(E-mail)272450912@qq.com
基金资助:
SHI Zhiyong1(), Aisajan Mamat2, Xianmisiya Tayifu3, CAO Yijie1, Yusuf Ablitif1()
Received:
2022-09-10
Online:
2023-04-20
Published:
2023-05-06
Correspondence author:
Yusuf Ablitif(1969-), male, Urumqi, Xinjiang, associate Professor, graduate supervisor.Research Fields: fruit tree cultivation and physiology,(E-mail)Supported by:
摘要:
【目的】研究库尔勒香梨上可能诱导粗皮果形成的胁迫因子。【方法】基于分析库尔勒香梨粗皮果形成的基础上,对生长期的库尔勒香梨枝条采用50%程度地摘叶、外源喷施250 mmol/L的H2O22种人为逆境处理,在成熟期统计处理枝条上粗皮果率,对不同处理的果实进行组织切片染色,检测生理指标。【结果】库尔勒香梨成熟期对照组的粗皮果比率为18.10%,H2O2喷施和摘叶处理的粗皮果率分别为51.93%、29.52%;3组果实中果皮中的木质素含量分别为3.4%、4.9%、3.9%,果肉中的木质素含量分别为1.5%、1.7%、2.0%,2个处理组均提高了库尔勒香梨的粗皮果率以及果实中的木质素含量。H2O2喷施后外果皮的丙二醛(MDA)和活性氧(H2O2和$O^{-}_{2}$)含量显著增加,分别为对照的1.7和2.9倍。与之相应,库尔勒香梨果皮可溶性糖、淀粉等渗透调节物质含量也显著增加,分别为对照的1.3和1.4倍。摘叶处理后库尔勒香梨果肉中可溶性糖、淀粉等渗透调节物质的含量也显著提高,分别为对照组果肉的1.2和2.0倍。【结论】2种人为逆境处理能有效提高粗皮果的比率,并且提高果实内部单位视野中的石细胞数量和木质素含量。其中H2O2喷施组主要对库尔勒香梨的果皮部分产生影响;摘叶组主要对库尔勒香梨的果肉部分产生影响。
中图分类号:
史智勇, 艾沙江·买买提, 仙米斯娅·塔依甫, 曹艺洁, 玉苏甫·阿不力提甫. 摘叶和喷施外源过氧化氢对库尔勒香梨粗皮果形成的影响[J]. 新疆农业科学, 2023, 60(4): 889-896.
SHI Zhiyong, Aisajan Mamat, Xianmisiya Tayifu, CAO Yijie, Yusuf Ablitif. Study on the induction of rough-skinned fruit formation in Korla fragrant pear[J]. Xinjiang Agricultural Sciences, 2023, 60(4): 889-896.
图2 库尔勒香梨生长地区1 d的温度和光照 注:A图为日光照变化;B图为日温度变化
Fig.2 Daily temperature and light conditions in Korla fragrant pear growing area Note: figure A shows the change of sunlight; Figure B shows the daily temperature change
图3 不同处理组库尔勒香梨果皮的活性氧含量变化 注:数据(平均值±标准差)后用不同小写字母表示在P<0.05 差异显著;无标注即代表没有显著性差异,下同
Fig.3 ROS content of Korla fragrant pear pericarp in different treatment groups Note: Values (Mean ± SE) are represented by different small letters with significant difference at P<0.05;And no annotation represents no significant difference,the same as below
图4 库尔勒香梨果实不同处理组丙二醛含量变化 注:数据(平均值±标准差)后用不同小写字母表示在P<0.05 差异显著
Fig.4 MDA content of different treatment groups of Korla fragrant pear Note: Values (Mean ± SE) are represented by different small letters with significant difference at P<0.05
实验组名称 Name of experimental group | 胁迫类型 Stress type | 粗皮果率 Rough skin fruits rate (%) |
---|---|---|
CK组 | 对照 | 18.10±6.94b |
H组 | 活性氧胁迫 | 51.93±13.87a |
Y组 | 光照胁迫 | 29.52±3.43ab |
表1 库尔勒香梨结果枝不同处理组的粗皮果率变化
Tab.1 Rough skin fruits percentage of Korla fragrant pear fruit bearing branches in different treatment groups
实验组名称 Name of experimental group | 胁迫类型 Stress type | 粗皮果率 Rough skin fruits rate (%) |
---|---|---|
CK组 | 对照 | 18.10±6.94b |
H组 | 活性氧胁迫 | 51.93±13.87a |
Y组 | 光照胁迫 | 29.52±3.43ab |
图6 库尔勒香梨不同处理组果实石细胞的显微变化 注:A~C. CK,H,Y组的果实果皮附近染色图,1、2、3分别为外果皮部位同一切片上的全景扫描图的3个连续片段3段不同部位;a~c.处理组CK,H,Y组的果实果肉部分染色图
Fig.6 Microscopic observation of the stone cell different treatment groups in Korla fragrant pear Note:A-C represents the staining diagram near the fruit peel of CK, H and Y groups. Figures1, 2 and 3 are the three different parts of the same piece respectively. a-c represents staining of fruit pulp in treatment group CK, H and Y, respectively
图7 库尔勒香梨不同处理组果实木质素、纤维素、半纤维素含量变化 注:A、B、C分别为果皮、果肉中木质素含量、纤维素含量、半纤维素含量;数据(平均值±标准差)后用不同小写字母表示在P<0.05 差异显著
Fig.7 Cellulose and hemicellulose content of fruits from different treatment groups of Korla fragrant pear Note: A. B and C are lignin content, cellulose content and hemicellulose content in peel and pulp, respectively; Values (Mean ± SE) are represented by different small letters with significant difference at P<0.05
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